Restriction map for
pF1KB4321
(Circular) (Six-base) MAPSORT of: pF1KB4321.seq Check: 1967 from: 1 to: 6125
pF1KB4321 6125 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1303 2149 1303
Size: 846 5279
AccI GT'mk_AC
Cuts at: 791 3055 3163 791
Size: 2264 108 3753
Fragments arranged by size:
3753 2264 108
Acc65I G'GTAC_C
Cuts at: 1346 3141 1346
Size: 1795 4330
AclI AA'CG_TT
Cuts at: 5965 5965
Size: 6125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1691 1743 2956 3950 4382 4735 5397 1691
Size: 52 1213 994 432 353 662 2419
Fragments arranged by size:
2419 1213 994 662 432 353 52
AflIII A'CryG_T
Cuts at: 1271 2900 3477 5277 5558 1271
Size: 1629 577 1800 281 1838
Fragments arranged by size:
1838 1800 1629 577 281
AleI CACnn'nnGTG
Cuts at: 1528 1789 1528
Size: 261 5864
AlwNI CAG_nnn'CTG
Cuts at: 3430 4868 3430
Size: 1438 4687
ApaI G_GGCC'C
Cuts at: 125 125
Size: 6125
ApaLI G'TGCA_C
Cuts at: 4963 4963
Size: 6125
ApoI r'AATT_y
Cuts at: 291 499 1724 2104 2164 2627 3129 5478 5734
Size: 208 1225 380 60 463 502 2349 256
Cuts at: 5734 291
Size: 682
Fragments arranged by size:
2349 1225 682 502 463 380 256 208
60
AseI AT'TA_AT
Cuts at: 20 20
Size: 6125
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
AvaI C'yCGr_G
Cuts at: 117 125 2576 2596 3145 117
Size: 8 2451 20 549 3097
Fragments arranged by size:
3097 2451 549 20 8
AvrII C'CTAG_G
Cuts at: 2274 2274
Size: 6125
BamHI G'GATC_C
Cuts at: 2082 3150 2082
Size: 1068 5057
BanI G'GyrC_C
Cuts at: 957 1006 1346 3141 3555 3803 3838 957
Size: 49 340 1795 414 248 35 3244
Fragments arranged by size:
3244 1795 414 340 248 49 35
BanII G_rGCy'C
Cuts at: 118 125 222 1462 1470 3139 4169 118
Size: 7 97 1240 8 1669 1030 2074
Fragments arranged by size:
2074 1669 1240 1030 97 8 7
BbeI G_GCGC'C
Cuts at: 3559 3807 3559
Size: 248 5877
BbsI GAAGACnn'nnnn_
Cuts at: 825 1710 1749 2944 825
Size: 885 39 1195 4006
Fragments arranged by size:
4006 1195 885 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6091
BciVI GTATCCnnnnn_n'
Cuts at: 3073 4023 5079 6125 3073
Size: 950 1056 1046 3073
Fragments arranged by size:
3073 1056 1046 950
BclI T'GATC_A
Cuts at: 585 2304 3645 585
Size: 1719 1341 3065
Fragments arranged by size:
3065 1719 1341
BfrBI ATG'CAT
Cuts at: 974 2016 2953 974
Size: 1042 937 4146
Fragments arranged by size:
4146 1042 937
BglII A'GATC_T
Cuts at: 2508 3640 5677 2508
Size: 1132 2037 2956
Fragments arranged by size:
2956 2037 1132
BlpI GC'TnA_GC
Cuts at: 3237 3237
Size: 6125
Bme1580I G_kGCm'C
Cuts at: 125 962 2033 3750 3843 4967 125
Size: 837 1071 1717 93 1124 1283
Fragments arranged by size:
1717 1283 1124 1071 837 93
BmgBI CAC'GTC
Cuts at: 233 1272 233
Size: 1039 5086
BmrI ACTGGGnnnn_n'
Cuts at: 547 1783 2757 3337 3418 3753 5897 547
Size: 1236 974 580 81 335 2144 775
Fragments arranged by size:
2144 1236 974 775 580 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2403 4492 2403
Size: 2089 4036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 872 1502 2715 3023 551
Size: 321 630 1213 308 3653
Fragments arranged by size:
3653 1213 630 321 308
Bpu10I CC'TnA_GC
Cuts at: 2111 3104 2111
Size: 993 5132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1476 3301 4180 4894 5192 1476
Size: 1825 879 714 298 2409
Fragments arranged by size:
2409 1825 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1636 2733 5829 1636
Size: 1097 3096 1932
Fragments arranged by size:
3096 1932 1097
BsaAI yAC'GTr
Cuts at: 1735 2920 3478 1735
Size: 1185 558 4382
Fragments arranged by size:
4382 1185 558
BsaBI GATnn'nnATC
Cuts at: 6 2087 3639 5676 6
Size: 2081 1552 2037 455
Fragments arranged by size:
2081 2037 1552 455
BsaHI Gr'CG_yC
Cuts at: 138 1883 3556 3804 4506 6002 138
Size: 1745 1673 248 702 1496 261
Fragments arranged by size:
1745 1673 1496 702 261 248
BsaWI w'CCGG_w
Cuts at: 257 3314 3835 4513 4924 5071 5345 257
Size: 3057 521 678 411 147 274 1037
Fragments arranged by size:
3057 1037 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1178 1339 1879 1178
Size: 161 540 5424
Fragments arranged by size:
5424 540 161
BseYI C'CCAG_C
Cuts at: 180 277 3550 4308 4973 5381 180
Size: 97 3273 758 665 408 924
Fragments arranged by size:
3273 924 758 665 408 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 1041 191
Size: 850 5275
BsiEI CG_ry'CG
Cuts at: 104 3667 3713 4943 104
Size: 3563 46 1230 1286
Fragments arranged by size:
3563 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1734 3139 3917 4107 4967 1734
Size: 1405 778 190 860 2892
Fragments arranged by size:
2892 1405 860 778 190
BsmI GAATG_Cn'
Cuts at: 1200 1200
Size: 6125
Bsp1286I G_dGCh'C
Cuts at: 118 125 222 962 1462 1470 1734 2033 3139
Size: 7 97 740 500 8 264 299 1106
Cuts at: 3139 3750 3843 3917 4107 4169 4967 118
Size: 611 93 74 190 62 798 1276
Fragments arranged by size:
1276 1106 798 740 611 500 299 264
190 97 93 74 62 8 7
BspEI T'CCGG_A
Cuts at: 257 257
Size: 6125
BspHI T'CATG_A
Cuts at: 1385 4557 1385
Size: 3172 2953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1303 2149 3175 3389 3691 4072 1303
Size: 846 1026 214 302 381 3356
Fragments arranged by size:
3356 1026 846 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4417 4471 5955 6120 50
Size: 4367 54 1484 165 55
Fragments arranged by size:
4367 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 288 635 767 4037 288
Size: 347 132 3270 2376
Fragments arranged by size:
3270 2376 347 132
BsrFI r'CCGG_y
Cuts at: 140 193 3050 4123 4304 140
Size: 53 2857 1073 181 1961
Fragments arranged by size:
2857 1961 1073 181 53
BssSI C'ACGA_G
Cuts at: 4396 5104 4396
Size: 708 5417
BstAPI GCAn_nnn'nTGC
Cuts at: 1298 3502 1298
Size: 2204 3921
BstBI TT'CG_AA
Cuts at: 4486 4486
Size: 6125
BstEII G'GTnAC_C
Cuts at: 673 2337 3108 673
Size: 1664 771 3690
Fragments arranged by size:
3690 1664 771
BstXI CCAn_nnnn'nTGG
Cuts at: 2639 2639
Size: 6125
BstYI r'GATC_y
Cuts at: 1 458 1395 1995 2082 2508 2998 3150 3617
Size: 457 937 600 87 426 490 152 467
Cuts at: 3617 3640 3975 4221 4527 4539 4625 5677 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 937 600 490 467 457 449 426
335 306 246 152 87 86 23 12
BstZ17I GTA'TAC
Cuts at: 792 3056 792
Size: 2264 3861
BtgI C'CryG_G
Cuts at: 2810 2810
Size: 6125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4075 4256 4419 4075
Size: 181 163 5781
Fragments arranged by size:
5781 181 163
BtsI GCAGTG_nn'
Cuts at: 930 1442 1877 3368 930
Size: 512 435 1491 3687
Fragments arranged by size:
3687 1491 512 435
DraI TTT'AAA
Cuts at: 3124 3124
Size: 6125
DrdI GACnn_nn'nnGTC
Cuts at: 3831 5175 3831
Size: 1344 4781
EaeI y'GGCC_r
Cuts at: 191 269 3710 3884 4275 4302 5647 191
Size: 78 3441 174 391 27 1345 669
Fragments arranged by size:
3441 1345 669 391 174 78 27
EagI C'GGCC_G
Cuts at: 3710 3710
Size: 6125
EarI CTCTTCn'nnn_
Cuts at: 353 989 1997 4148 4358 353
Size: 636 1008 2151 210 2120
Fragments arranged by size:
2151 2120 1008 636 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1232 4333 5075 5221 5935 1232
Size: 3101 742 146 714 1422
Fragments arranged by size:
3101 1422 742 714 146
EcoICRI GAG'CTC
Cuts at: 3137 3137
Size: 6125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 872 1502 1691 1743 2715 2956 3023 3950
Size: 321 630 189 52 972 241 67 927
Cuts at: 3950 4382 4735 5397 551
Size: 432 353 662 1279
Fragments arranged by size:
1279 972 927 662 630 432 353 321
241 189 67 52
EcoO109I rG'GnC_Cy
Cuts at: 121 122 321 542 715 3264 121
Size: 1 199 221 173 2549 2982
Fragments arranged by size:
2982 2549 221 199 173 1
EcoRI G'AATT_C
Cuts at: 3129 3129
Size: 6125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 573 4144 573
Size: 3571 2554
FspI TGC'GCA
Cuts at: 3906 3906
Size: 6125
HaeII r_GCGC'y
Cuts at: 941 3559 3807 5037 5808 941
Size: 2618 248 1230 771 1258
Fragments arranged by size:
2618 1258 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1380 1412 2745 2777 3967 3997 3999 4029 1380
Size: 32 1333 32 1190 30 2 30 3476
Fragments arranged by size:
3476 1333 1190 32 32 30 30 2
HincII GTy'rAC
Cuts at: 897 2898 3164 897
Size: 2001 266 3858
Fragments arranged by size:
3858 2001 266
HindIII A'AGCT_T
Cuts at: 651 1100 651
Size: 449 5676
KasI G'GCGC_C
Cuts at: 3555 3803 3555
Size: 248 5877
KpnI G_GTAC'C
Cuts at: 1350 3145 1350
Size: 1795 4330
MfeI C'AATT_G
Cuts at: 868 868
Size: 6125
MluI A'CGCG_T
Cuts at: 5558 5558
Size: 6125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 436 1475 2885 4884 5068 436
Size: 1039 1410 1999 184 1493
Fragments arranged by size:
1999 1493 1410 1039 184
MscI TGG'CCA
Cuts at: 271 3886 271
Size: 3615 2510
MslI CAynn'nnrTG
Cuts at: 313 1313 1390 1528 1789 2075 4114 4241 313
Size: 1000 77 138 261 286 2039 127 2197
Fragments arranged by size:
2197 2039 1000 286 261 138 127 77
MspA1I CmG'CkG
Cuts at: 3236 3550 3910 4694 4939 3236
Size: 314 360 784 245 4422
Fragments arranged by size:
4422 784 360 314 245
NaeI GCC'GGC
Cuts at: 142 195 4306 142
Size: 53 4111 1961
Fragments arranged by size:
4111 1961 53
NarI GG'CG_CC
Cuts at: 3556 3804 3556
Size: 248 5877
NcoI C'CATG_G
Cuts at: 2810 2810
Size: 6125
NgoMIV G'CCGG_C
Cuts at: 140 193 4304 140
Size: 53 4111 1961
Fragments arranged by size:
4111 1961 53
NsiI A_TGCA'T
Cuts at: 976 2018 2955 976
Size: 1042 937 4146
Fragments arranged by size:
4146 1042 937
NspI r_CATG'y
Cuts at: 978 1169 1292 2451 2904 3178 3678 5281 978
Size: 191 123 1159 453 274 500 1603 1822
Fragments arranged by size:
1822 1603 1159 500 453 274 191 123
PciI A'CATG_T
Cuts at: 2900 5277 2900
Size: 2377 3748
PflMI CCAn_nnn'nTGG
Cuts at: 349 349
Size: 6125
PfoI T'CCnGG_A
Cuts at: 528 528
Size: 6125
PmeI GTTT'AAAC
Cuts at: 3124 3124
Size: 6125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 4571
Size: 32 6093
PpuMI rG'GwC_Cy
Cuts at: 321 542 715 321
Size: 221 173 5731
Fragments arranged by size:
5731 221 173
PspOMI G'GGCC_C
Cuts at: 121 121
Size: 6125
PstI C_TGCA'G
Cuts at: 937 1689 2469 2962 3172 3857 937
Size: 752 780 493 210 685 3205
Fragments arranged by size:
3205 780 752 685 493 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6125
PvuII CAG'CTG
Cuts at: 3550 3910 3550
Size: 360 5765
RsrII CG'GwC_CG
Cuts at: 4320 4320
Size: 6125
SacI G_AGCT'C
Cuts at: 3139 3139
Size: 6125
SalI G'TCGA_C
Cuts at: 3162 3162
Size: 6125
SapI GCTCTTCn'nnn_
Cuts at: 4148 4358 4148
Size: 210 5915
SbfI CC_TGCA'GG
Cuts at: 3172 3172
Size: 6125
ScaI AGT'ACT
Cuts at: 5642 5642
Size: 6125
SfcI C'TryA_G
Cuts at: 33 933 1685 1977 2465 2958 3009 3168 3853
Size: 900 752 292 488 493 51 159 685
Cuts at: 3853 4821 5012 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 900 752 685 493 488 292
191 159 51
SfoI GGC'GCC
Cuts at: 3557 3805 3557
Size: 248 5877
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6125
SgrAI Cr'CCGG_yG
Cuts at: 140 140
Size: 6125
SmaI CCC'GGG
Cuts at: 127 2578 3147 127
Size: 2451 569 3105
Fragments arranged by size:
3105 2451 569
SmlI C'TyrA_G
Cuts at: 1455 3280 4195 4909 5171 1455
Size: 1825 915 714 262 2409
Fragments arranged by size:
2409 1825 915 714 262
SnaBI TAC'GTA
Cuts at: 2920 2920
Size: 6125
SpeI A'CTAG_T
Cuts at: 67 1219 67
Size: 1152 4973
SphI G_CATG'C
Cuts at: 1169 1292 2451 3178 3678 1169
Size: 123 1159 727 500 3616
Fragments arranged by size:
3616 1159 727 500 123
StyI C'CwwG_G
Cuts at: 2274 2810 3113 3259 3613 2274
Size: 536 303 146 354 4786
Fragments arranged by size:
4786 536 354 303 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 341 925 1170 3708 3841 4300 4509 341
Size: 584 245 2538 133 459 209 1957
Fragments arranged by size:
2538 1957 584 459 245 209 133
TatI w'GTAC_w
Cuts at: 5640 5640
Size: 6125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1037 1069 5642 5674 1037
Size: 32 4573 32 1488
Fragments arranged by size:
4573 1488 32 32
Tth111I GACn'n_nGTC
Cuts at: 3922 3922
Size: 6125
XbaI T'CTAG_A
Cuts at: 3156 3156
Size: 6125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 350 1961 3620 350
Size: 1611 1659 2855
Fragments arranged by size:
2855 1659 1611
XmaI C'CCGG_G
Cuts at: 125 2576 3145 125
Size: 2451 569 3105
Fragments arranged by size:
3105 2451 569
XmnI GAAnn'nnTTC
Cuts at: 1238 1238
Size: 6125
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
BaeI BbvCI BglI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SrfI SspI StuI SwaI XhoI ZraI