Restriction map for
pF1KB4336
(Circular) (Six-base) MAPSORT of: pF1KB4336.seq Check: 3399 from: 1 to: 6392
pF1KB4336 6392 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 176 543 3008 3430 176
Size: 367 2465 422 3138
Fragments arranged by size:
3138 2465 422 367
Acc65I G'GTAC_C
Cuts at: 3130 3408 3130
Size: 278 6114
AclI AA'CG_TT
Cuts at: 6232 6232
Size: 6392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 1223 1516 2296 2911 4217 4649 5396 5664
Size: 590 293 780 615 1306 432 747 268
Cuts at: 5664 633
Size: 1361
Fragments arranged by size:
1361 1306 780 747 615 590 432 293
268
AflII C'TTAA_G
Cuts at: 850 850
Size: 6392
AflIII A'CryG_T
Cuts at: 191 360 3744 4848 5825 191
Size: 169 3384 1104 977 758
Fragments arranged by size:
3384 1104 977 758 169
AgeI A'CCGG_T
Cuts at: 4780 4780
Size: 6392
AhdI GACnn_n'nnGTC
Cuts at: 1389 3271 1389
Size: 1882 4510
AleI CACnn'nnGTG
Cuts at: 1462 1939 1462
Size: 477 5915
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2654 2654
Size: 6392
AlwNI CAG_nnn'CTG
Cuts at: 2161 2226 2313 2328 2967 3054 3157 3235 3697
Size: 65 87 15 639 87 103 78 462
Cuts at: 3697 5264 2161
Size: 1567 3289
Fragments arranged by size:
3289 1567 639 462 103 87 87 78
65 15
ApaI G_GGCC'C
Cuts at: 1130 1322 1365 1585 1853 2441 2670 2868 1130
Size: 192 43 220 268 588 229 198 4654
Fragments arranged by size:
4654 588 268 229 220 198 192 43
ApaLI G'TGCA_C
Cuts at: 5162 5162
Size: 6392
ApoI r'AATT_y
Cuts at: 3396 5745 6001 3396
Size: 2349 256 3787
Fragments arranged by size:
3787 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6392
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6392
AvaI C'yCGr_G
Cuts at: 385 507 1264 1364 2568 2716 3412 385
Size: 122 757 100 1204 148 696 3365
Fragments arranged by size:
3365 1204 757 696 148 122 100
BamHI G'GATC_C
Cuts at: 3417 3417
Size: 6392
BanI G'GyrC_C
Cuts at: 237 1368 1426 2005 2712 3130 3408 3822 4070
Size: 1131 58 579 707 418 278 414 248
Cuts at: 4070 4105 237
Size: 35 2524
Fragments arranged by size:
2524 1131 707 579 418 414 278 248
58 35
BanII G_rGCy'C
Cuts at: 422 1130 1162 1271 1322 1365 1585 1853 2441
Size: 708 32 109 51 43 220 268 588
Cuts at: 2441 2497 2638 2670 2804 2868 3073 3186 3406
Size: 56 141 32 134 64 205 113 220
Cuts at: 3406 4436 422
Size: 1030 2378
Fragments arranged by size:
2378 1030 708 588 268 220 220 205
141 134 113 109 64 56 51 43
32 32
BbeI G_GCGC'C
Cuts at: 1372 3826 4074 1372
Size: 2454 248 3690
Fragments arranged by size:
3690 2454 248
BbsI GAAGACnn'nnnn_
Cuts at: 667 1007 1296 2102 667
Size: 340 289 806 4957
Fragments arranged by size:
4957 806 340 289
BbvCI CC'TCA_GC
Cuts at: 1257 1879 2750 3301 3366 1257
Size: 622 871 551 65 4283
Fragments arranged by size:
4283 871 622 551 65
BciVI GTATCCnnnnn_n'
Cuts at: 4290 5051 6392 4290
Size: 761 1341 4290
Fragments arranged by size:
4290 1341 761
BclI T'GATC_A
Cuts at: 3912 3912
Size: 6392
BglI GCCn_nnn'nGGC
Cuts at: 574 1615 2858 3190 574
Size: 1041 1243 332 3776
Fragments arranged by size:
3776 1243 1041 332
BglII A'GATC_T
Cuts at: 3907 5944 3907
Size: 2037 4355
BlpI GC'TnA_GC
Cuts at: 3504 3504
Size: 6392
Bme1580I G_kGCm'C
Cuts at: 191 313 360 375 570 1130 1322 1365 1576
Size: 122 47 15 195 560 192 43 211
Cuts at: 1576 1585 1611 1853 2387 2441 2670 2715 2868
Size: 9 26 242 534 54 229 45 153
Cuts at: 2868 4017 4110 5166 191
Size: 1149 93 1056 1417
Fragments arranged by size:
1417 1149 1056 560 534 242 229 211
195 192 153 122 93 54 47 45
43 26 15 9
BmgBI CAC'GTC
Cuts at: 558 1401 558
Size: 843 5549
BmrI ACTGGGnnnn_n'
Cuts at: 1586 1598 3604 3685 4020 6164 1586
Size: 12 2006 81 335 2144 1814
Fragments arranged by size:
2144 2006 1814 335 81 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4759 4759
Size: 6392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 722 1210 1332 1673 1756 1821 2080 2248
Size: 97 488 122 341 83 65 259 168
Cuts at: 2248 2611 2932 2941 3200 3254 625
Size: 363 321 9 259 54 3763
Fragments arranged by size:
3763 488 363 341 321 259 259 168
122 97 83 65 54 9
Bpu10I CC'TnA_GC
Cuts at: 1257 1353 1879 2329 2750 3301 3366 1257
Size: 96 526 450 421 551 65 4283
Fragments arranged by size:
4283 551 526 450 421 96 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1749 2499 2868 3568 4447 4939 5237 1749
Size: 750 369 700 879 492 298 2904
Fragments arranged by size:
2904 879 750 700 492 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 933 970 1471 6096 933
Size: 37 501 4625 1229
Fragments arranged by size:
4625 1229 501 37
BsaAI yAC'GTr
Cuts at: 363 438 3745 363
Size: 75 3307 3010
Fragments arranged by size:
3307 3010 75
BsaBI GATnn'nnATC
Cuts at: 6 3906 5943 6
Size: 3900 2037 455
Fragments arranged by size:
3900 2037 455
BsaHI Gr'CG_yC
Cuts at: 1369 2255 3823 4071 4773 6269 1369
Size: 886 1568 248 702 1496 1492
Fragments arranged by size:
1568 1496 1492 886 702 248
BsaWI w'CCGG_w
Cuts at: 2075 3581 4102 4780 5054 5201 5612 2075
Size: 1506 521 678 274 147 411 2855
Fragments arranged by size:
2855 1506 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 913 943 1195 1198 1225 1228 1771 1801 2092
Size: 30 252 3 27 3 543 30 291
Cuts at: 2092 2122 3173 3203 913
Size: 30 1051 30 4102
Fragments arranged by size:
4102 1051 543 291 252 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 493 707 719 722 768 921 924 1080 1092
Size: 214 12 3 46 153 3 156 12
Cuts at: 1092 1095 1104 1107 1216 1539 1824 1902 2011
Size: 3 9 3 109 323 285 78 109
Cuts at: 2011 2614 493
Size: 603 4271
Fragments arranged by size:
4271 603 323 285 214 156 153 109
109 78 46 12 12 9 3 3
3 3
BseYI C'CCAG_C
Cuts at: 155 353 886 1584 1610 1723 2687 2730 3064
Size: 198 533 698 26 113 964 43 334
Cuts at: 3064 3074 3817 4575 5152 5648 155
Size: 10 743 758 577 496 899
Fragments arranged by size:
964 899 758 743 698 577 533 496
334 198 113 43 26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 2147 2269 2392 216
Size: 1931 122 123 4216
Fragments arranged by size:
4216 1931 123 122
BsiEI CG_ry'CG
Cuts at: 104 3934 3980 5188 104
Size: 3830 46 1208 1308
Fragments arranged by size:
3830 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 410 1162 2485 3406 4184 4374 5166 410
Size: 752 1323 921 778 190 792 1636
Fragments arranged by size:
1636 1323 921 792 778 752 190
BsmI GAATG_Cn'
Cuts at: 1177 1177
Size: 6392
Bsp1286I G_dGCh'C
Cuts at: 191 313 360 375 410 422 570 1130 1162
Size: 122 47 15 35 12 148 560 32
Cuts at: 1162 1271 1322 1365 1576 1585 1611 1853 2387
Size: 109 51 43 211 9 26 242 534
Cuts at: 2387 2441 2485 2497 2638 2670 2715 2804 2868
Size: 54 44 12 141 32 45 89 64
Cuts at: 2868 3073 3186 3406 4017 4110 4184 4374 4436
Size: 205 113 220 611 93 74 190 62
Cuts at: 4436 5166 191
Size: 730 1417
Fragments arranged by size:
1417 730 611 560 534 242 220 211
205 190 148 141 122 113 109 93
89 74 64 62 54 51 47 45
44 43 35 32 32 26 15 12
12 9
BspEI T'CCGG_A
Cuts at: 5612 5612
Size: 6392
BspHI T'CATG_A
Cuts at: 444 5568 444
Size: 5124 1268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3442 3656 3958 4339 3442
Size: 214 302 381 5495
Fragments arranged by size:
5495 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1886 2352 4684 4738 6222 6387 50
Size: 1836 466 2332 54 1484 165 55
Fragments arranged by size:
2332 1836 1484 466 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4304 4304
Size: 6392
BsrFI r'CCGG_y
Cuts at: 761 2251 2461 4390 4571 4780 761
Size: 1490 210 1929 181 209 2373
Fragments arranged by size:
2373 1929 1490 210 209 181
BssSI C'ACGA_G
Cuts at: 808 958 4663 5021 808
Size: 150 3705 358 2179
Fragments arranged by size:
3705 2179 358 150
BstAPI GCAn_nnn'nTGC
Cuts at: 438 1453 2292 3054 3235 3769 438
Size: 1015 839 762 181 534 3061
Fragments arranged by size:
3061 1015 839 762 534 181
BstBI TT'CG_AA
Cuts at: 4753 4753
Size: 6392
BstXI CCAn_nnnn'nTGG
Cuts at: 1618 1845 1990 2745 3096 1618
Size: 227 145 755 351 4914
Fragments arranged by size:
4914 755 351 227 145
BstYI r'GATC_y
Cuts at: 1 2324 3026 3417 3884 3907 4242 4488 5500
Size: 2323 702 391 467 23 335 246 1012
Cuts at: 5500 5586 5598 5944 1
Size: 86 12 346 449
Fragments arranged by size:
2323 1012 702 467 449 391 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2096 2096
Size: 6392
BtgI C'CryG_G
Cuts at: 413 534 905 1533 2000 2744 3083 413
Size: 121 371 628 467 744 339 3722
Fragments arranged by size:
3722 744 628 467 371 339 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 197 4342 4523 4686 197
Size: 4145 181 163 1903
Fragments arranged by size:
4145 1903 181 163
BtsI GCAGTG_nn'
Cuts at: 741 914 1062 1631 2784 3278 3330 3635 741
Size: 173 148 569 1153 494 52 305 3498
Fragments arranged by size:
3498 1153 569 494 305 173 148 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 199 234 199
Size: 35 6357
DraI TTT'AAA
Cuts at: 3391 3391
Size: 6392
DraIII CAC_nnn'GTG
Cuts at: 366 1453 2170 366
Size: 1087 717 4588
Fragments arranged by size:
4588 1087 717
DrdI GACnn_nn'nnGTC
Cuts at: 4098 4956 4098
Size: 858 5534
EaeI y'GGCC_r
Cuts at: 203 2178 2339 3086 3224 3977 4151 4542 4569
Size: 1975 161 747 138 753 174 391 27
Cuts at: 4569 5914 203
Size: 1345 681
Fragments arranged by size:
1975 1345 753 747 681 391 174 161
138 27
EagI C'GGCC_G
Cuts at: 3977 3977
Size: 6392
EarI CTCTTCn'nnn_
Cuts at: 328 820 951 1098 1113 1920 2149 4415 4625
Size: 492 131 147 15 807 229 2266 210
Cuts at: 4625 328
Size: 2095
Fragments arranged by size:
2266 2095 807 492 229 210 147 131
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 704 1074 3278 4600 4910 5056 6202 704
Size: 370 2204 1322 310 146 1146 894
Fragments arranged by size:
2204 1322 1146 894 370 310 146
EcoICRI GAG'CTC
Cuts at: 1160 3404 1160
Size: 2244 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 633 722 1210 1223 1332 1516 1673 1756
Size: 8 89 488 13 109 184 157 83
Cuts at: 1756 1821 2080 2248 2296 2611 2911 2932 2941
Size: 65 259 168 48 315 300 21 9
Cuts at: 2941 3200 3254 4217 4649 5396 5664 625
Size: 259 54 963 432 747 268 1353
Fragments arranged by size:
1353 963 747 488 432 315 300 268
259 259 184 168 157 109 89 83
65 54 48 21 13 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1487 1487
Size: 6392
EcoO109I rG'GnC_Cy
Cuts at: 247 1126 1127 1415 1581 1779 1849 1850 1859
Size: 879 1 288 166 198 70 1 9
Cuts at: 1859 2087 2438 2550 2666 2667 2784 2864 2865
Size: 228 351 112 116 1 117 80 1
Cuts at: 2865 3531 247
Size: 666 3108
Fragments arranged by size:
3108 879 666 351 288 228 198 166
117 116 112 80 70 9 1 1
1 1
EcoRI G'AATT_C
Cuts at: 3396 3396
Size: 6392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 865 1320 4411 865
Size: 455 3091 2846
Fragments arranged by size:
3091 2846 455
FspI TGC'GCA
Cuts at: 4173 4173
Size: 6392
HaeII r_GCGC'y
Cuts at: 701 1372 2284 3054 3826 4074 5096 6075 701
Size: 671 912 770 772 248 1022 979 1018
Fragments arranged by size:
1022 1018 979 912 772 770 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 913 945 1198 1230 1375 1407 1771 1803 2079
Size: 32 253 32 145 32 364 32 276
Cuts at: 2079 2092 2111 2124 2542 2574 3257 3289 4234
Size: 13 19 13 418 32 683 32 945
Cuts at: 4234 4264 4266 4296 913
Size: 30 2 30 3009
Fragments arranged by size:
3009 945 683 418 364 276 253 145
32 32 32 32 32 32 30 30
19 13 13 2
HincII GTy'rAC
Cuts at: 544 2259 3431 544
Size: 1715 1172 3505
Fragments arranged by size:
3505 1715 1172
HindIII A'AGCT_T
Cuts at: 215 548 2844 215
Size: 333 2296 3763
Fragments arranged by size:
3763 2296 333
KasI G'GCGC_C
Cuts at: 1368 3822 4070 1368
Size: 2454 248 3690
Fragments arranged by size:
3690 2454 248
KpnI G_GTAC'C
Cuts at: 3134 3412 3134
Size: 278 6114
MluI A'CGCG_T
Cuts at: 191 5825 191
Size: 5634 758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5063 5247 5063
Size: 184 6208
MscI TGG'CCA
Cuts at: 3088 4153 3088
Size: 1065 5327
MslI CAynn'nnrTG
Cuts at: 1462 1939 2743 4381 4508 1462
Size: 477 804 1638 127 3346
Fragments arranged by size:
3346 1638 804 477 127
MspA1I CmG'CkG
Cuts at: 171 680 1588 1614 2310 2616 2725 2827 2880
Size: 509 908 26 696 306 109 102 53
Cuts at: 2880 2982 3078 3117 3178 3235 3241 3352 3503
Size: 102 96 39 61 57 6 111 151
Cuts at: 3503 3817 4177 5190 5435 171
Size: 314 360 1013 245 1128
Fragments arranged by size:
1128 1013 908 696 509 360 314 306
245 151 111 109 102 102 96 61
57 53 39 26 6
NaeI GCC'GGC
Cuts at: 2253 2463 4573 2253
Size: 210 2110 4072
Fragments arranged by size:
4072 2110 210
NarI GG'CG_CC
Cuts at: 1369 3823 4071 1369
Size: 2454 248 3690
Fragments arranged by size:
3690 2454 248
NcoI C'CATG_G
Cuts at: 413 534 1533 2000 2744 3083 413
Size: 121 999 467 744 339 3722
Fragments arranged by size:
3722 999 744 467 339 121
NgoMIV G'CCGG_C
Cuts at: 2251 2461 4571 2251
Size: 210 2110 4072
Fragments arranged by size:
4072 2110 210
NspI r_CATG'y
Cuts at: 3445 3945 4852 3445
Size: 500 907 4985
Fragments arranged by size:
4985 907 500
PasI CC'CwG_GG
Cuts at: 1378 1855 2200 2434 1378
Size: 477 345 234 5336
Fragments arranged by size:
5336 477 345 234
PciI A'CATG_T
Cuts at: 4848 4848
Size: 6392
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 6392
PmeI GTTT'AAAC
Cuts at: 3391 3391
Size: 6392
PmlI CAC'GTG
Cuts at: 363 363
Size: 6392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5531 5563 5531
Size: 32 6360
PpuMI rG'GwC_Cy
Cuts at: 247 1779 1859 2087 2550 247
Size: 1532 80 228 463 4089
Fragments arranged by size:
4089 1532 463 228 80
PshAI GACnn'nnGTC
Cuts at: 3004 3004
Size: 6392
PspOMI G'GGCC_C
Cuts at: 1126 1318 1361 1581 1849 2437 2666 2864 1126
Size: 192 43 220 268 588 229 198 4654
Fragments arranged by size:
4654 588 268 229 220 198 192 43
PstI C_TGCA'G
Cuts at: 434 1143 1157 1822 2071 2297 2360 2879 3050
Size: 709 14 665 249 226 63 519 171
Cuts at: 3050 3059 3240 3246 3439 4124 434
Size: 9 181 6 193 685 2702
Fragments arranged by size:
2702 709 685 665 519 249 226 193
181 171 63 14 9 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6392
PvuII CAG'CTG
Cuts at: 1588 1614 2880 2982 3078 3235 3241 3817 4177
Size: 26 1266 102 96 157 6 576 360
Cuts at: 4177 1588
Size: 3803
Fragments arranged by size:
3803 1266 576 360 157 102 96 26
6
RsrII CG'GwC_CG
Cuts at: 4587 4587
Size: 6392
SacI G_AGCT'C
Cuts at: 1162 3406 1162
Size: 2244 4148
SalI G'TCGA_C
Cuts at: 542 3429 542
Size: 2887 3505
SanDI GG'GwC_CC
Cuts at: 247 247
Size: 6392
SapI GCTCTTCn'nnn_
Cuts at: 4415 4625 4415
Size: 210 6182
SbfI CC_TGCA'GG
Cuts at: 3439 3439
Size: 6392
ScaI AGT'ACT
Cuts at: 625 1213 5909 625
Size: 588 4696 1108
Fragments arranged by size:
4696 1108 588
SfcI C'TryA_G
Cuts at: 33 430 1139 1153 1818 2067 2293 2356 2875
Size: 397 709 14 665 249 226 63 519
Cuts at: 2875 3046 3055 3236 3242 3435 4120 5113 5304
Size: 171 9 181 6 193 685 993 191
Cuts at: 5304 33
Size: 1121
Fragments arranged by size:
1121 993 709 685 665 519 397 249
226 193 191 181 171 63 14 9
6
SfoI GGC'GCC
Cuts at: 1370 3824 4072 1370
Size: 2454 248 3690
Fragments arranged by size:
3690 2454 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6392
SmaI CCC'GGG
Cuts at: 1366 2718 3414 1366
Size: 1352 696 4344
Fragments arranged by size:
4344 1352 696
SmlI C'TyrA_G
Cuts at: 850 1728 2478 2847 3547 4462 4954 5216 850
Size: 878 750 369 700 915 492 262 2026
Fragments arranged by size:
2026 915 878 750 700 492 369 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6392
SphI G_CATG'C
Cuts at: 3445 3945 3445
Size: 500 5892
SrfI GCCC'GGGC
Cuts at: 1366 1366
Size: 6392
StuI AGG'CCT
Cuts at: 1493 1493
Size: 6392
StyI C'CwwG_G
Cuts at: 413 534 1274 1533 1550 2000 2744 2805 3083
Size: 121 740 259 17 450 744 61 278
Cuts at: 3083 3526 3880 413
Size: 443 354 2925
Fragments arranged by size:
2925 744 740 450 443 354 278 259
121 61 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 817 1444 1993 3975 4108 4567 4776 817
Size: 627 549 1982 133 459 209 2433
Fragments arranged by size:
2433 1982 627 549 459 209 133
TatI w'GTAC_w
Cuts at: 623 1211 5907 623
Size: 588 4696 1108
Fragments arranged by size:
4696 1108 588
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 5909 5941 3173
Size: 32 2704 32 3624
Fragments arranged by size:
3624 2704 32 32
Tth111I GACn'n_nGTC
Cuts at: 616 925 4189 616
Size: 309 3264 2819
Fragments arranged by size:
3264 2819 309
XbaI T'CTAG_A
Cuts at: 3423 3423
Size: 6392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 220 248 1611 1731 2649 3887 114
Size: 106 28 1363 120 918 1238 2619
Fragments arranged by size:
2619 1363 1238 918 120 106 28
XmaI C'CCGG_G
Cuts at: 1364 2716 3412 1364
Size: 1352 696 4344
Fragments arranged by size:
4344 1352 696
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI