Restriction map for
pF1KB4351
(Circular) (Six-base) MAPSORT of: pF1KB4351.seq Check: 1707 from: 1 to: 4193
pF1KB4351 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 147 147
Size: 4193
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 539 4033 539
Size: 3494 699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 2018 2450 2803 3465 324
Size: 1694 432 353 662 1052
Fragments arranged by size:
1694 1052 662 432 353
AflIII A'CryG_T
Cuts at: 676 1545 3345 3626 676
Size: 869 1800 281 1243
Fragments arranged by size:
1800 1243 869 281
AlwNI CAG_nnn'CTG
Cuts at: 1029 1498 2936 1029
Size: 469 1438 2286
Fragments arranged by size:
2286 1438 469
ApaI G_GGCC'C
Cuts at: 139 139
Size: 4193
ApaLI G'TGCA_C
Cuts at: 3031 3031
Size: 4193
ApoI r'AATT_y
Cuts at: 708 876 1197 3546 3802 708
Size: 168 321 2349 256 1099
Fragments arranged by size:
2349 1099 321 256 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1062 1213 1062
Size: 151 4042
BamHI G'GATC_C
Cuts at: 841 1218 841
Size: 377 3816
BanI G'GyrC_C
Cuts at: 208 924 1209 1623 1871 1906 208
Size: 716 285 414 248 35 2495
Fragments arranged by size:
2495 716 414 285 248 35
BanII G_rGCy'C
Cuts at: 139 693 1076 1207 2237 139
Size: 554 383 131 1030 2095
Fragments arranged by size:
2095 1030 554 383 131
BbeI G_GCGC'C
Cuts at: 212 1627 1875 212
Size: 1415 248 2530
Fragments arranged by size:
2530 1415 248
BbsI GAAGACnn'nnnn_
Cuts at: 463 863 463
Size: 400 3793
BbvCI CC'TCA_GC
Cuts at: 469 469
Size: 4193
BciVI GTATCCnnnnn_n'
Cuts at: 1171 2091 3147 4193 1171
Size: 920 1056 1046 1171
Fragments arranged by size:
1171 1056 1046 920
BclI T'GATC_A
Cuts at: 642 1713 642
Size: 1071 3122
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4193
BglII A'GATC_T
Cuts at: 438 599 1183 1708 3745 438
Size: 161 584 525 2037 886
Fragments arranged by size:
2037 886 584 525 161
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 139 1818 1911 3035 139
Size: 1679 93 1124 1297
Fragments arranged by size:
1679 1297 1124 93
BmgBI CAC'GTC
Cuts at: 168 168
Size: 4193
BmrI ACTGGGnnnn_n'
Cuts at: 1405 1486 1821 3965 1405
Size: 81 335 2144 1633
Fragments arranged by size:
2144 1633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 1066 677
Size: 389 3804
Bpu10I CC'TnA_GC
Cuts at: 469 469
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 580 1369 2248 2962 3260 541
Size: 39 789 879 714 298 1474
Fragments arranged by size:
1474 879 789 714 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 325 846 1182 1707 3744 6
Size: 319 521 336 525 2037 455
Fragments arranged by size:
2037 525 521 455 336 319
BsaHI Gr'CG_yC
Cuts at: 209 1624 1872 2574 4070 209
Size: 1415 248 702 1496 332
Fragments arranged by size:
1496 1415 702 332 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2992 3139 3413 1382
Size: 521 678 411 147 274 2162
Fragments arranged by size:
2162 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1618 2376 3041 3449 1618
Size: 758 665 408 2362
Fragments arranged by size:
2362 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 529
Size: 4193
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 3011 104
Size: 1631 46 1230 1286
Fragments arranged by size:
1631 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 229 290 384 1207 1985 2175 3035 229
Size: 61 94 823 778 190 860 1387
Fragments arranged by size:
1387 860 823 778 190 94 61
BsmI GAATG_Cn'
Cuts at: 755 755
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 139 229 290 384 693 1076 1207 1818 1911
Size: 90 61 94 309 383 131 611 93
Cuts at: 1911 1985 2175 2237 3035 139
Size: 74 190 62 798 1297
Fragments arranged by size:
1297 798 611 383 309 190 131 94
93 90 74 62 61
BspHI T'CATG_A
Cuts at: 2625 2625
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 147 578 1243 1457 1759 2140 147
Size: 431 665 214 302 381 2200
Fragments arranged by size:
2200 665 431 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2485 2539 4023 4188 50
Size: 2435 54 1484 165 55
Fragments arranged by size:
2435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 480 2105 480
Size: 1625 2568
BsrFI r'CCGG_y
Cuts at: 2191 2372 2191
Size: 181 4012
BssSI C'ACGA_G
Cuts at: 2464 3172 2464
Size: 708 3485
BstAPI GCAn_nnn'nTGC
Cuts at: 224 1570 224
Size: 1346 2847
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 966 966
Size: 4193
BstYI r'GATC_y
Cuts at: 1 438 503 599 630 841 1183 1218 1685
Size: 437 65 96 31 211 342 35 467
Cuts at: 1685 1708 2043 2289 2595 2607 2693 3745 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 467 449 437 342 335 306 246
211 96 86 65 35 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 313 1124 313
Size: 811 3382
BtgI C'CryG_G
Cuts at: 107 150 928 107
Size: 43 778 3372
Fragments arranged by size:
3372 778 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1062 2143 2324 2487 1062
Size: 1081 181 163 2768
Fragments arranged by size:
2768 1081 181 163
BtsI GCAGTG_nn'
Cuts at: 1436 1436
Size: 4193
DraI TTT'AAA
Cuts at: 881 1192 881
Size: 311 3882
DrdI GACnn_nn'nnGTC
Cuts at: 1899 3243 1899
Size: 1344 2849
EaeI y'GGCC_r
Cuts at: 671 1778 1952 2343 2370 3715 671
Size: 1107 174 391 27 1345 1149
Fragments arranged by size:
1345 1149 1107 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 911 1148 2216 2426 911
Size: 237 1068 210 2678
Fragments arranged by size:
2678 1068 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 3143 3289 4003 2401
Size: 742 146 714 2591
Fragments arranged by size:
2591 742 714 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 677 1066 2018 2450 2803 3465 324
Size: 353 389 952 432 353 662 1052
Fragments arranged by size:
1052 952 662 432 389 353 353
EcoO109I rG'GnC_Cy
Cuts at: 423 1332 423
Size: 909 3284
EcoRI G'AATT_C
Cuts at: 708 1197 708
Size: 489 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 4193
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 212 1627 1875 3105 3876 212
Size: 1415 248 1230 771 529
Fragments arranged by size:
1415 1230 771 529 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 727 759 2035 2065 2067 2097 248
Size: 32 447 32 1276 30 2 30 2344
Fragments arranged by size:
2344 1276 447 32 32 30 30 2
HincII GTy'rAC
Cuts at: 246 989 1232 246
Size: 743 243 3207
Fragments arranged by size:
3207 743 243
KasI G'GCGC_C
Cuts at: 208 1623 1871 208
Size: 1415 248 2530
Fragments arranged by size:
2530 1415 248
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 2952 3136 590
Size: 2362 184 1647
Fragments arranged by size:
2362 1647 184
MscI TGG'CCA
Cuts at: 673 1954 673
Size: 1281 2912
MslI CAynn'nnrTG
Cuts at: 2182 2309 2182
Size: 127 4066
MspA1I CmG'CkG
Cuts at: 152 816 1304 1618 1978 2762 3007 152
Size: 664 488 314 360 784 245 1338
Fragments arranged by size:
1338 784 664 488 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 209 1624 1872 209
Size: 1415 248 2530
Fragments arranged by size:
2530 1415 248
NcoI C'CATG_G
Cuts at: 107 928 107
Size: 821 3372
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 3349 1246
Size: 500 1603 2090
Fragments arranged by size:
2090 1603 500
PciI A'CATG_T
Cuts at: 3345 3345
Size: 4193
PflMI CCAn_nnn'nTGG
Cuts at: 680 828 680
Size: 148 4045
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 423 423
Size: 4193
PspOMI G'GGCC_C
Cuts at: 135 135
Size: 4193
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 816 1618 1978 816
Size: 802 360 3031
Fragments arranged by size:
3031 802 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SacII CC_GC'GG
Cuts at: 153 153
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 746 1236 1921 2889 3080 33
Size: 713 490 685 968 191 1146
Fragments arranged by size:
1146 968 713 685 490 191
SfoI GGC'GCC
Cuts at: 210 1625 1873 210
Size: 1415 248 2530
Fragments arranged by size:
2530 1415 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 556 595 1348 2263 2977 3239 556
Size: 39 753 915 714 262 1510
Fragments arranged by size:
1510 915 753 714 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 107 713 928 1327 1681 107
Size: 606 215 399 354 2619
Fragments arranged by size:
2619 606 399 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1052 1776 1909 2368 2577 1052
Size: 724 133 459 209 2668
Fragments arranged by size:
2668 724 459 209 133
TatI w'GTAC_w
Cuts at: 417 3708 417
Size: 3291 902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BmtI BsaXI BseRI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI