Restriction map for
pF1KB4360
(Circular) (Six-base) MAPSORT of: pF1KB4360.seq Check: 685 from: 1 to: 5498
pF1KB4360 5498 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1604 1604
Size: 5498
AatII G_ACGT'C
Cuts at: 1436 1436
Size: 5498
AccI GT'mk_AC
Cuts at: 1153 2168 2536 1153
Size: 1015 368 4115
Fragments arranged by size:
4115 1015 368
Acc65I G'GTAC_C
Cuts at: 2514 2514
Size: 5498
AclI AA'CG_TT
Cuts at: 5338 5338
Size: 5498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 3323 3755 4108 4770 2425
Size: 898 432 353 662 3153
Fragments arranged by size:
3153 898 662 432 353
AfeI AGC'GCT
Cuts at: 704 704
Size: 5498
AflIII A'CryG_T
Cuts at: 2850 4650 4931 2850
Size: 1800 281 3417
Fragments arranged by size:
3417 1800 281
AleI CACnn'nnGTG
Cuts at: 1312 1312
Size: 5498
AlwNI CAG_nnn'CTG
Cuts at: 556 2803 4241 556
Size: 2247 1438 1813
Fragments arranged by size:
2247 1813 1438
ApaI G_GGCC'C
Cuts at: 2296 2296
Size: 5498
ApaLI G'TGCA_C
Cuts at: 4336 4336
Size: 5498
ApoI r'AATT_y
Cuts at: 630 829 1004 2502 4851 5107 630
Size: 199 175 1498 2349 256 1021
Fragments arranged by size:
2349 1498 1021 256 199 175
AseI AT'TA_AT
Cuts at: 20 20
Size: 5498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 290 862 905 1835 1890 1939 2287 2518 290
Size: 572 43 930 55 49 348 231 3270
Fragments arranged by size:
3270 930 572 348 231 55 49 43
BamHI G'GATC_C
Cuts at: 2523 2523
Size: 5498
BanI G'GyrC_C
Cuts at: 858 1546 1812 1830 2283 2326 2514 2928 3176
Size: 688 266 18 453 43 188 414 248
Cuts at: 3176 3211 858
Size: 35 3145
Fragments arranged by size:
3145 688 453 414 266 248 188 43
35 18
BanII G_rGCy'C
Cuts at: 329 731 765 1940 2296 2512 3542 329
Size: 402 34 1175 356 216 1030 2285
Fragments arranged by size:
2285 1175 1030 402 356 216 34
BbeI G_GCGC'C
Cuts at: 1816 2932 3180 1816
Size: 1116 248 4134
Fragments arranged by size:
4134 1116 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2171 2205 2171
Size: 34 5464
BciVI GTATCCnnnnn_n'
Cuts at: 1802 3396 4452 5498 1802
Size: 1594 1056 1046 1802
Fragments arranged by size:
1802 1594 1056 1046
BclI T'GATC_A
Cuts at: 3018 3018
Size: 5498
BfrBI ATG'CAT
Cuts at: 306 306
Size: 5498
BglI GCCn_nnn'nGGC
Cuts at: 250 1678 250
Size: 1428 4070
BglII A'GATC_T
Cuts at: 3013 5050 3013
Size: 2037 3461
BlpI GC'TnA_GC
Cuts at: 730 1726 1993 2610 730
Size: 996 267 617 3618
Fragments arranged by size:
3618 996 617 267
Bme1580I G_kGCm'C
Cuts at: 863 1835 2288 2296 3123 3216 4340 863
Size: 972 453 8 827 93 1124 2021
Fragments arranged by size:
2021 1124 972 827 453 93 8
BmgBI CAC'GTC
Cuts at: 2319 2319
Size: 5498
BmrI ACTGGGnnnn_n'
Cuts at: 2710 2791 3126 5270 2710
Size: 81 335 2144 2938
Fragments arranged by size:
2938 2144 335 81
BmtI G_CTAG'C
Cuts at: 124 124
Size: 5498
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 204 3865 204
Size: 3661 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1146 1146
Size: 5498
Bpu10I CC'TnA_GC
Cuts at: 725 725
Size: 5498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1909 2674 3553 4267 4565 1909
Size: 765 879 714 298 2842
Fragments arranged by size:
2842 879 765 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1431 1620 5202 1431
Size: 189 3582 1727
Fragments arranged by size:
3582 1727 189
BsaAI yAC'GTr
Cuts at: 2082 2851 2082
Size: 769 4729
BsaBI GATnn'nnATC
Cuts at: 6 3012 5049 6
Size: 3006 2037 455
Fragments arranged by size:
3006 2037 455
BsaHI Gr'CG_yC
Cuts at: 1433 1763 1813 2929 3177 3879 5375 1433
Size: 330 50 1116 248 702 1496 1556
Fragments arranged by size:
1556 1496 1116 702 330 248 50
BsaWI w'CCGG_w
Cuts at: 2371 2471 2687 3208 3886 4297 4444 4718 2371
Size: 100 216 521 678 411 147 274 3151
Fragments arranged by size:
3151 678 521 411 274 216 147 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 1699 1729 2086 2116 376
Size: 30 1293 30 357 30 3758
Fragments arranged by size:
3758 1293 357 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 730 2265 730
Size: 1535 3963
BseYI C'CCAG_C
Cuts at: 545 2105 2923 3681 4346 4754 545
Size: 1560 818 758 665 408 1289
Fragments arranged by size:
1560 1289 818 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 731 1492 2062 2265 469
Size: 262 761 570 203 3702
Fragments arranged by size:
3702 761 570 262 203
BsiEI CG_ry'CG
Cuts at: 104 1844 2270 2274 3040 3086 4316 104
Size: 1740 426 4 766 46 1230 1286
Fragments arranged by size:
1740 1286 1230 766 426 46 4
BsiHKAI G_wGCw'C
Cuts at: 329 544 731 1409 2087 2222 2512 3290 3480
Size: 215 187 678 678 135 290 778 190
Cuts at: 3480 4340 329
Size: 860 1487
Fragments arranged by size:
1487 860 778 678 678 290 215 190
187 135
BsmI GAATG_Cn'
Cuts at: 308 308
Size: 5498
Bsp1286I G_dGCh'C
Cuts at: 329 544 731 765 863 1409 1835 1940 2087
Size: 215 187 34 98 546 426 105 147
Cuts at: 2087 2222 2288 2296 2512 3123 3216 3290 3480
Size: 135 66 8 216 611 93 74 190
Cuts at: 3480 3542 4340 329
Size: 62 798 1487
Fragments arranged by size:
1487 798 611 546 426 216 215 190
187 147 135 105 98 93 74 66
62 34 8
BspEI T'CCGG_A
Cuts at: 2471 2471
Size: 5498
BspHI T'CATG_A
Cuts at: 3930 3930
Size: 5498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1604 2123 2548 2762 3064 3445 1604
Size: 519 425 214 302 381 3657
Fragments arranged by size:
3657 519 425 381 302 214
BsrBI CCG'CTC
Cuts at: 50 720 904 2324 3790 3844 5328 5493 50
Size: 670 184 1420 1466 54 1484 165 55
Fragments arranged by size:
1484 1466 1420 670 184 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3410 3410
Size: 5498
BsrFI r'CCGG_y
Cuts at: 349 2386 3496 3677 349
Size: 2037 1110 181 2170
Fragments arranged by size:
2170 2037 1110 181
BssHII G'CGCG_C
Cuts at: 296 296
Size: 5498
BssSI C'ACGA_G
Cuts at: 3769 4477 3769
Size: 708 4790
BstAPI GCAn_nnn'nTGC
Cuts at: 2875 2875
Size: 5498
BstBI TT'CG_AA
Cuts at: 3859 3859
Size: 5498
BstEII G'GTnAC_C
Cuts at: 1560 1860 1560
Size: 300 5198
BstXI CCAn_nnnn'nTGG
Cuts at: 1872 2440 1872
Size: 568 4930
BstYI r'GATC_y
Cuts at: 1 2523 2990 3013 3348 3594 3900 3912 3998
Size: 2522 467 23 335 246 306 12 86
Cuts at: 3998 5050 1
Size: 1052 449
Fragments arranged by size:
2522 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1651 1651
Size: 5498
BtgI C'CryG_G
Cuts at: 107 1710 1931 107
Size: 1603 221 3674
Fragments arranged by size:
3674 1603 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 974 3448 3629 3792 974
Size: 2474 181 163 2680
Fragments arranged by size:
2680 2474 181 163
BtsI GCAGTG_nn'
Cuts at: 743 1524 2240 2741 743
Size: 781 716 501 3500
Fragments arranged by size:
3500 781 716 501
DraI TTT'AAA
Cuts at: 2497 2497
Size: 5498
DrdI GACnn_nn'nnGTC
Cuts at: 3204 4548 3204
Size: 1344 4154
EaeI y'GGCC_r
Cuts at: 242 284 1635 1841 2009 2063 3083 3257 3648
Size: 42 1351 206 168 54 1020 174 391
Cuts at: 3648 3675 5020 242
Size: 27 1345 720
Fragments arranged by size:
1351 1345 1020 720 391 206 174 168
54 42 27
EagI C'GGCC_G
Cuts at: 1841 3083 1841
Size: 1242 4256
EarI CTCTTCn'nnn_
Cuts at: 2209 3521 3731 2209
Size: 1312 210 3976
Fragments arranged by size:
3976 1312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 755 3706 4448 4594 5308 755
Size: 2951 742 146 714 945
Fragments arranged by size:
2951 945 742 714 146
EcoICRI GAG'CTC
Cuts at: 327 729 2510 327
Size: 402 1781 3315
Fragments arranged by size:
3315 1781 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1146 2425 3323 3755 4108 4770 1146
Size: 1279 898 432 353 662 1874
Fragments arranged by size:
1874 1279 898 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1080 1671 2292 2293 2333 2637 1080
Size: 591 621 1 40 304 3941
Fragments arranged by size:
3941 621 591 304 40 1
EcoRI G'AATT_C
Cuts at: 2502 2502
Size: 5498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 5498
FspI TGC'GCA
Cuts at: 1978 3279 1978
Size: 1301 4197
FspAI rTGC'GCAy
Cuts at: 1978 1978
Size: 5498
HaeII r_GCGC'y
Cuts at: 706 1529 1726 1741 1816 2441 2932 3180 4410
Size: 823 197 15 75 625 491 248 1230
Cuts at: 4410 5181 706
Size: 771 1023
Fragments arranged by size:
1230 1023 823 771 625 491 248 197
75 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1210 1242 1525 1557 1699 1731 1873 1905 2086
Size: 32 283 32 142 32 142 32 181
Cuts at: 2086 2118 3340 3370 3372 3402 1210
Size: 32 1222 30 2 30 3306
Fragments arranged by size:
3306 1222 283 181 142 142 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2537 2537
Size: 5498
KasI G'GCGC_C
Cuts at: 1812 2928 3176 1812
Size: 1116 248 4134
Fragments arranged by size:
4134 1116 248
KpnI G_GTAC'C
Cuts at: 2518 2518
Size: 5498
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1454 4257 4441 1454
Size: 2803 184 2511
Fragments arranged by size:
2803 2511 184
MscI TGG'CCA
Cuts at: 244 286 3259 244
Size: 42 2973 2483
Fragments arranged by size:
2973 2483 42
MslI CAynn'nnrTG
Cuts at: 641 1312 1378 3487 3614 641
Size: 671 66 2109 127 2525
Fragments arranged by size:
2525 2109 671 127 66
MspA1I CmG'CkG
Cuts at: 1712 1797 1920 2121 2609 2923 3283 4067 4312
Size: 85 123 201 488 314 360 784 245
Cuts at: 4312 1712
Size: 2898
Fragments arranged by size:
2898 784 488 360 314 245 201 123
85
NaeI GCC'GGC
Cuts at: 2388 3679 2388
Size: 1291 4207
NarI GG'CG_CC
Cuts at: 1813 2929 3177 1813
Size: 1116 248 4134
Fragments arranged by size:
4134 1116 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5498
NdeI CA'TA_TG
Cuts at: 927 927
Size: 5498
NgoMIV G'CCGG_C
Cuts at: 2386 3677 2386
Size: 1291 4207
NheI G'CTAG_C
Cuts at: 120 120
Size: 5498
NsiI A_TGCA'T
Cuts at: 308 308
Size: 5498
NspI r_CATG'y
Cuts at: 1956 2551 3051 4654 1956
Size: 595 500 1603 2800
Fragments arranged by size:
2800 1603 595 500
PasI CC'CwG_GG
Cuts at: 2433 2433
Size: 5498
PciI A'CATG_T
Cuts at: 4650 4650
Size: 5498
PfoI T'CCnGG_A
Cuts at: 775 775
Size: 5498
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PmlI CAC'GTG
Cuts at: 2082 2082
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2415 3944 3976 2383
Size: 32 1529 32 3905
Fragments arranged by size:
3905 1529 32 32
PpuMI rG'GwC_Cy
Cuts at: 2333 2333
Size: 5498
PshAI GACnn'nnGTC
Cuts at: 263 263
Size: 5498
PsiI TTA'TAA
Cuts at: 1049 1049
Size: 5498
PspOMI G'GGCC_C
Cuts at: 2292 2292
Size: 5498
PstI C_TGCA'G
Cuts at: 473 1679 1802 2545 3230 473
Size: 1206 123 743 685 2741
Fragments arranged by size:
2741 1206 743 685 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 1920 2923 3283 1920
Size: 1003 360 4135
Fragments arranged by size:
4135 1003 360
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 329 731 2512 329
Size: 402 1781 3315
Fragments arranged by size:
3315 1781 402
SacII CC_GC'GG
Cuts at: 1713 1713
Size: 5498
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SanDI GG'GwC_CC
Cuts at: 2333 2333
Size: 5498
SapI GCTCTTCn'nnn_
Cuts at: 2209 3521 3731 2209
Size: 1312 210 3976
Fragments arranged by size:
3976 1312 210
SbfI CC_TGCA'GG
Cuts at: 1679 2545 1679
Size: 866 4632
ScaI AGT'ACT
Cuts at: 5015 5015
Size: 5498
SexAI A'CCwGG_T
Cuts at: 1856 2171 1856
Size: 315 5183
SfcI C'TryA_G
Cuts at: 33 469 1106 1193 1675 1798 2541 3226 4194
Size: 436 637 87 482 123 743 685 968
Cuts at: 4194 4385 33
Size: 191 1146
Fragments arranged by size:
1146 968 743 685 637 482 436 191
123 87
SfoI GGC'GCC
Cuts at: 1814 2930 3178 1814
Size: 1116 248 4134
Fragments arranged by size:
4134 1116 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SmaI CCC'GGG
Cuts at: 1837 1941 2520 1837
Size: 104 579 4815
Fragments arranged by size:
4815 579 104
SmlI C'TyrA_G
Cuts at: 905 1924 2653 3568 4282 4544 905
Size: 1019 729 915 714 262 1859
Fragments arranged by size:
1859 1019 915 729 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 2551 3051 2551
Size: 500 4998
SrfI GCCC'GGGC
Cuts at: 1941 1941
Size: 5498
StyI C'CwwG_G
Cuts at: 107 954 1865 2632 2986 107
Size: 847 911 767 354 2619
Fragments arranged by size:
2619 911 847 767 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 1404 1474 2260 3081 3214 3673 3882 559
Size: 845 70 786 821 133 459 209 2175
Fragments arranged by size:
2175 845 821 786 459 209 133 70
TatI w'GTAC_w
Cuts at: 1352 5013 1352
Size: 3661 1837
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 5015 5047 376
Size: 32 4607 32 827
Fragments arranged by size:
4607 827 32 32
Tth111I GACn'n_nGTC
Cuts at: 3295 3295
Size: 5498
XbaI T'CTAG_A
Cuts at: 2529 2529
Size: 5498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2019 2993 2019
Size: 974 4524
XhoI C'TCGA_G
Cuts at: 905 905
Size: 5498
XmaI C'CCGG_G
Cuts at: 1835 1939 2518 1835
Size: 104 579 4815
Fragments arranged by size:
4815 579 104
ZraI GAC'GTC
Cuts at: 1434 1434
Size: 5498
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BsiWI BsmBI BsrGI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NotI
NruI PacI PflMI PspXI PsrI SfiI SgrAI SnaBI
SspI StuI SwaI XmnI