Restriction map for
pF1KB4384
(Circular) (Six-base) MAPSORT of: pF1KB4384.seq Check: 976 from: 1 to: 5723
pF1KB4384 5723 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 710 2761 710
Size: 2051 3672
Acc65I G'GTAC_C
Cuts at: 2739 2739
Size: 5723
AclI AA'CG_TT
Cuts at: 5563 5563
Size: 5723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1583 1615 1782 2245 2251 3548 3980 4333 4995
Size: 32 167 463 6 1297 432 353 662
Cuts at: 4995 1583
Size: 2311
Fragments arranged by size:
2311 1297 662 463 432 353 167 32
6
AflIII A'CryG_T
Cuts at: 590 1055 3075 4875 5156 590
Size: 465 2020 1800 281 1157
Fragments arranged by size:
2020 1800 1157 465 281
AhdI GACnn_n'nnGTC
Cuts at: 980 980
Size: 5723
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 850 882 1762 1794 850
Size: 32 880 32 4779
Fragments arranged by size:
4779 880 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1541 1827 1881 2271 3028 4466 1541
Size: 286 54 390 757 1438 2798
Fragments arranged by size:
2798 1438 757 390 286 54
ApaI G_GGCC'C
Cuts at: 2054 2114 2594 2054
Size: 60 480 5183
Fragments arranged by size:
5183 480 60
ApaLI G'TGCA_C
Cuts at: 627 996 4561 627
Size: 369 3565 1789
Fragments arranged by size:
3565 1789 369
ApoI r'AATT_y
Cuts at: 943 2727 5076 5332 943
Size: 1784 2349 256 1334
Fragments arranged by size:
2349 1784 1334 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5723
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
AvaI C'yCGr_G
Cuts at: 164 1297 1407 1500 1747 1903 2174 2479 2587
Size: 1133 110 93 247 156 271 305 108
Cuts at: 2587 2743 164
Size: 156 3144
Fragments arranged by size:
3144 1133 305 271 247 156 156 110
108 93
BamHI G'GATC_C
Cuts at: 2748 2748
Size: 5723
BanI G'GyrC_C
Cuts at: 179 1695 2739 3153 3401 3436 179
Size: 1516 1044 414 248 35 2466
Fragments arranged by size:
2466 1516 1044 414 248 35
BanII G_rGCy'C
Cuts at: 253 758 1298 1904 2054 2114 2175 2594 2703
Size: 505 540 606 150 60 61 419 109
Cuts at: 2703 2737 3767 253
Size: 34 1030 2209
Fragments arranged by size:
2209 1030 606 540 505 419 150 109
61 60 34
BbeI G_GCGC'C
Cuts at: 3157 3405 3157
Size: 248 5475
BbsI GAAGACnn'nnnn_
Cuts at: 1571 1727 1900 1571
Size: 156 173 5394
Fragments arranged by size:
5394 173 156
BbvCI CC'TCA_GC
Cuts at: 1380 1380
Size: 5723
BciVI GTATCCnnnnn_n'
Cuts at: 3621 4677 5723 3621
Size: 1056 1046 3621
Fragments arranged by size:
3621 1056 1046
BclI T'GATC_A
Cuts at: 3243 3243
Size: 5723
BglI GCCn_nnn'nGGC
Cuts at: 1315 2109 1315
Size: 794 4929
BglII A'GATC_T
Cuts at: 3238 5275 3238
Size: 2037 3686
BlpI GC'TnA_GC
Cuts at: 933 1732 2835 933
Size: 799 1103 3821
Fragments arranged by size:
3821 1103 799
Bme1580I G_kGCm'C
Cuts at: 631 1000 1487 2054 2114 2594 2654 3348 3441
Size: 369 487 567 60 480 60 694 93
Cuts at: 3441 4565 631
Size: 1124 1789
Fragments arranged by size:
1789 1124 694 567 487 480 369 93
60 60
BmgBI CAC'GTC
Cuts at: 1161 2187 1161
Size: 1026 4697
BmrI ACTGGGnnnn_n'
Cuts at: 1104 1511 2013 2520 2935 3016 3351 5495 1104
Size: 407 502 507 415 81 335 2144 1332
Fragments arranged by size:
2144 1332 507 502 415 407 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1395 2707 4090 1395
Size: 1312 1383 3028
Fragments arranged by size:
3028 1383 1312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1903 2263 1084
Size: 819 360 4544
Fragments arranged by size:
4544 819 360
Bpu10I CC'TnA_GC
Cuts at: 841 1380 2272 841
Size: 539 892 4292
Fragments arranged by size:
4292 892 539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 2899 3778 4492 4790 646
Size: 2253 879 714 298 1579
Fragments arranged by size:
2253 1579 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1060 5427 1060
Size: 4367 1356
BsaAI yAC'GTr
Cuts at: 1056 3076 1056
Size: 2020 3703
BsaBI GATnn'nnATC
Cuts at: 6 3237 5274 6
Size: 3231 2037 455
Fragments arranged by size:
3231 2037 455
BsaHI Gr'CG_yC
Cuts at: 1334 3154 3402 4104 5600 1334
Size: 1820 248 702 1496 1457
Fragments arranged by size:
1820 1496 1457 702 248
BsaWI w'CCGG_w
Cuts at: 1028 2912 3433 4111 4522 4669 4943 1028
Size: 1884 521 678 411 147 274 1808
Fragments arranged by size:
1884 1808 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 253 283 296 326 253
Size: 30 13 30 5650
Fragments arranged by size:
5650 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 304 309 544 716 931 1306 1315 1387
Size: 142 5 235 172 215 375 9 72
Cuts at: 1387 1449 1765 2683 162
Size: 62 316 918 3202
Fragments arranged by size:
3202 918 375 316 235 215 172 142
72 62 9 5
BseYI C'CCAG_C
Cuts at: 1310 1459 1787 2658 3148 3906 4571 4979 1310
Size: 149 328 871 490 758 665 408 2054
Fragments arranged by size:
2054 871 758 665 490 408 328 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 675
Size: 5723
BsiEI CG_ry'CG
Cuts at: 104 3265 3311 4541 104
Size: 3161 46 1230 1286
Fragments arranged by size:
3161 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 253 631 1000 1910 2737 3515 3705 4565 253
Size: 378 369 910 827 778 190 860 1411
Fragments arranged by size:
1411 910 860 827 778 378 369 190
BsmBI CGTCTCn'nnnn_
Cuts at: 190 1325 190
Size: 1135 4588
Bsp1286I G_dGCh'C
Cuts at: 253 631 758 1000 1298 1487 1904 1910 2054
Size: 378 127 242 298 189 417 6 144
Cuts at: 2054 2114 2175 2594 2654 2703 2737 3348 3441
Size: 60 61 419 60 49 34 611 93
Cuts at: 3441 3515 3705 3767 4565 253
Size: 74 190 62 798 1411
Fragments arranged by size:
1411 798 611 419 417 378 298 242
190 189 144 127 93 74 62 61
60 60 49 34 6
BspHI T'CATG_A
Cuts at: 614 4155 614
Size: 3541 2182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1109 2489 2618 2773 2987 3289 3670 1109
Size: 1380 129 155 214 302 381 3162
Fragments arranged by size:
3162 1380 381 302 214 155 129
BsrBI CCG'CTC
Cuts at: 50 603 932 1755 2276 4015 4069 5553 5718
Size: 553 329 823 521 1739 54 1484 165
Cuts at: 5718 50
Size: 55
Fragments arranged by size:
1739 1484 823 553 521 329 165 55
54
BsrDI GCAATG_nn'
Cuts at: 401 3635 401
Size: 3234 2489
BsrFI r'CCGG_y
Cuts at: 182 1910 2579 3721 3902 182
Size: 1728 669 1142 181 2003
Fragments arranged by size:
2003 1728 1142 669 181
BssSI C'ACGA_G
Cuts at: 3994 4702 3994
Size: 708 5015
BstAPI GCAn_nnn'nTGC
Cuts at: 1541 3100 1541
Size: 1559 4164
BstBI TT'CG_AA
Cuts at: 4084 4084
Size: 5723
BstEII G'GTnAC_C
Cuts at: 411 633 904 411
Size: 222 271 5230
Fragments arranged by size:
5230 271 222
BstYI r'GATC_y
Cuts at: 1 2008 2031 2748 3215 3238 3573 3819 4125
Size: 2007 23 717 467 23 335 246 306
Cuts at: 4125 4137 4223 5275 1
Size: 12 86 1052 449
Fragments arranged by size:
2007 1052 717 467 449 335 306 246
86 23 23 12
Bsu36I CC'TnA_GG
Cuts at: 914 2335 914
Size: 1421 4302
BtgI C'CryG_G
Cuts at: 407 1172 1417 1424 1625 1709 1997 2201 407
Size: 765 245 7 201 84 288 204 3929
Fragments arranged by size:
3929 765 288 245 204 201 84 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3673 3854 4017 3673
Size: 181 163 5379
Fragments arranged by size:
5379 181 163
BtsI GCAGTG_nn'
Cuts at: 687 1242 1791 2966 687
Size: 555 549 1175 3444
Fragments arranged by size:
3444 1175 555 549
DraI TTT'AAA
Cuts at: 2722 2722
Size: 5723
DraIII CAC_nnn'GTG
Cuts at: 246 2060 2293 2562 246
Size: 1814 233 269 3407
Fragments arranged by size:
3407 1814 269 233
DrdI GACnn_nn'nnGTC
Cuts at: 3429 4773 3429
Size: 1344 4379
EaeI y'GGCC_r
Cuts at: 927 1020 1211 1971 2198 3308 3482 3873 3900
Size: 93 191 760 227 1110 174 391 27
Cuts at: 3900 5245 927
Size: 1345 1405
Fragments arranged by size:
1405 1345 1110 760 391 227 191 174
93 27
EagI C'GGCC_G
Cuts at: 3308 3308
Size: 5723
EarI CTCTTCn'nnn_
Cuts at: 1255 1624 1998 2246 3746 3956 1255
Size: 369 374 248 1500 210 3022
Fragments arranged by size:
3022 1500 374 369 248 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 201 1780 3931 4673 4819 5533 201
Size: 1579 2151 742 146 714 391
Fragments arranged by size:
2151 1579 742 714 391 146
EcoICRI GAG'CTC
Cuts at: 251 2735 251
Size: 2484 3239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1583 1615 1782 1903 2245 2251 2263 3548
Size: 499 32 167 121 342 6 12 1285
Cuts at: 3548 3980 4333 4995 1084
Size: 432 353 662 1812
Fragments arranged by size:
1812 1285 662 499 432 353 342 167
121 32 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2707 2707
Size: 5723
EcoO109I rG'GnC_Cy
Cuts at: 2110 2111 2268 2283 2421 2591 2688 2862 2110
Size: 1 157 15 138 170 97 174 4971
Fragments arranged by size:
4971 174 170 157 138 97 15 1
EcoRI G'AATT_C
Cuts at: 943 2727 943
Size: 1784 3939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1284 3742 1284
Size: 2458 3265
FspI TGC'GCA
Cuts at: 3504 3504
Size: 5723
HaeII r_GCGC'y
Cuts at: 2104 3157 3405 4635 5406 2104
Size: 1053 248 1230 771 2421
Fragments arranged by size:
2421 1230 1053 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 240 253 272 285 304 482 514 966 998
Size: 13 19 13 19 178 32 452 32
Cuts at: 998 1322 1354 3565 3595 3597 3627 240
Size: 324 32 2211 30 2 30 2336
Fragments arranged by size:
2336 2211 452 324 178 32 32 32
30 30 19 19 13 13 2
HincII GTy'rAC
Cuts at: 657 1128 2762 657
Size: 471 1634 3618
Fragments arranged by size:
3618 1634 471
KasI G'GCGC_C
Cuts at: 3153 3401 3153
Size: 248 5475
KpnI G_GTAC'C
Cuts at: 2743 2743
Size: 5723
MluI A'CGCG_T
Cuts at: 5156 5156
Size: 5723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4482 4666 2240
Size: 2242 184 3297
Fragments arranged by size:
3297 2242 184
MscI TGG'CCA
Cuts at: 1022 1213 1973 3484 1022
Size: 191 760 1511 3261
Fragments arranged by size:
3261 1511 760 191
MslI CAynn'nnrTG
Cuts at: 406 619 778 859 3712 3839 406
Size: 213 159 81 2853 127 2290
Fragments arranged by size:
2853 2290 213 159 127 81
MspA1I CmG'CkG
Cuts at: 115 1314 1495 1541 1881 2241 2451 2834 3148
Size: 1199 181 46 340 360 210 383 314
Cuts at: 3148 3508 4292 4537 115
Size: 360 784 245 1301
Fragments arranged by size:
1301 1199 784 383 360 360 340 314
245 210 181 46
NaeI GCC'GGC
Cuts at: 184 2581 3904 184
Size: 2397 1323 2003
Fragments arranged by size:
2397 2003 1323
NarI GG'CG_CC
Cuts at: 3154 3402 3154
Size: 248 5475
NcoI C'CATG_G
Cuts at: 1172 1417 1625 1709 1997 2201 1172
Size: 245 208 84 288 204 4694
Fragments arranged by size:
4694 288 245 208 204 84
NgoMIV G'CCGG_C
Cuts at: 182 2579 3902 182
Size: 2397 1323 2003
Fragments arranged by size:
2397 2003 1323
NspI r_CATG'y
Cuts at: 594 2776 3276 4879 594
Size: 2182 500 1603 1438
Fragments arranged by size:
2182 1603 1438 500
PasI CC'CwG_GG
Cuts at: 1071 1071
Size: 5723
PciI A'CATG_T
Cuts at: 590 4875 590
Size: 4285 1438
PflMI CCAn_nnn'nTGG
Cuts at: 366 855 1209 1827 2060 2293 366
Size: 489 354 618 233 233 3796
Fragments arranged by size:
3796 618 489 354 233 233
PfoI T'CCnGG_A
Cuts at: 1859 2264 1859
Size: 405 5318
PmeI GTTT'AAAC
Cuts at: 2722 2722
Size: 5723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 5691
PpuMI rG'GwC_Cy
Cuts at: 2268 2283 2688 2268
Size: 15 405 5303
Fragments arranged by size:
5303 405 15
PspOMI G'GGCC_C
Cuts at: 2050 2110 2590 2050
Size: 60 480 5183
Fragments arranged by size:
5183 480 60
PspXI vC'TCGA_Gb
Cuts at: 2174 2174
Size: 5723
PstI C_TGCA'G
Cuts at: 1106 2770 3455 1106
Size: 1664 685 3374
Fragments arranged by size:
3374 1664 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5723
PvuII CAG'CTG
Cuts at: 115 1314 1495 1541 1881 2241 3148 3508 115
Size: 1199 181 46 340 360 907 360 2330
Fragments arranged by size:
2330 1199 907 360 360 340 181 46
RsrII CG'GwC_CG
Cuts at: 3918 3918
Size: 5723
SacI G_AGCT'C
Cuts at: 253 2737 253
Size: 2484 3239
SalI G'TCGA_C
Cuts at: 2760 2760
Size: 5723
SanDI GG'GwC_CC
Cuts at: 2283 2283
Size: 5723
SapI GCTCTTCn'nnn_
Cuts at: 1624 2246 3746 3956 1624
Size: 622 1500 210 3391
Fragments arranged by size:
3391 1500 622 210
SbfI CC_TGCA'GG
Cuts at: 2770 2770
Size: 5723
ScaI AGT'ACT
Cuts at: 5240 5240
Size: 5723
SexAI A'CCwGG_T
Cuts at: 2289 2289
Size: 5723
SfcI C'TryA_G
Cuts at: 33 1102 1835 2538 2766 3451 4419 4610 33
Size: 1069 733 703 228 685 968 191 1146
Fragments arranged by size:
1146 1069 968 733 703 685 228 191
SfoI GGC'GCC
Cuts at: 3155 3403 3155
Size: 248 5475
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
SmaI CCC'GGG
Cuts at: 166 1502 2481 2745 166
Size: 1336 979 264 3144
Fragments arranged by size:
3144 1336 979 264
SmlI C'TyrA_G
Cuts at: 661 2174 2878 3793 4507 4769 661
Size: 1513 704 915 714 262 1615
Fragments arranged by size:
1615 1513 915 714 704 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5723
SphI G_CATG'C
Cuts at: 2776 3276 2776
Size: 500 5223
StuI AGG'CCT
Cuts at: 268 268
Size: 5723
StyI C'CwwG_G
Cuts at: 389 1001 1172 1417 1625 1640 1709 1997 2201
Size: 612 171 245 208 15 69 288 204
Cuts at: 2201 2857 3211 389
Size: 656 354 2901
Fragments arranged by size:
2901 656 612 354 288 245 208 204
171 69 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 815 1462 2419 2539 3306 3439 3898 4107 815
Size: 647 957 120 767 133 459 209 2431
Fragments arranged by size:
2431 957 767 647 459 209 133 120
TatI w'GTAC_w
Cuts at: 5238 5238
Size: 5723
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 296 328 1688 1720 5240 5272 296
Size: 32 1360 32 3520 32 747
Fragments arranged by size:
3520 1360 747 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2151 3520 2151
Size: 1369 4354
XbaI T'CTAG_A
Cuts at: 2754 2754
Size: 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 1344 1456 2421 3218 279
Size: 1065 112 965 797 2784
Fragments arranged by size:
2784 1065 965 797 112
XhoI C'TCGA_G
Cuts at: 2174 2174
Size: 5723
XmaI C'CCGG_G
Cuts at: 164 1500 2479 2743 164
Size: 1336 979 264 3144
Fragments arranged by size:
3144 1336 979 264
XmnI GAAnn'nnTTC
Cuts at: 1618 1618
Size: 5723
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BspEI
BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI