Restriction map for pF1KB4384
 (Circular) (Six-base) MAPSORT of: pF1KB4384.seq  Check: 976  from: 1  to: 5723



pF1KB4384  5723 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:    710    2761     710 
   Size:       2051    3672
 
Acc65I G'GTAC_C

Cuts at:   2739    2739 
   Size:       5723
 
AclI AA'CG_TT

Cuts at:   5563    5563 
   Size:       5723
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1583    1615    1782    2245    2251    3548    3980    4333    4995 
   Size:         32     167     463       6    1297     432     353     662

Cuts at:   4995    1583 
   Size:       2311

  Fragments arranged by size: 

                2311    1297     662     463     432     353     167      32
                   6
 
AflIII A'CryG_T

Cuts at:    590    1055    3075    4875    5156     590 
   Size:        465    2020    1800     281    1157

  Fragments arranged by size: 

                2020    1800    1157     465     281
 
AhdI GACnn_n'nnGTC

Cuts at:    980     980 
   Size:       5723
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    850     882    1762    1794     850 
   Size:         32     880      32    4779

  Fragments arranged by size: 

                4779     880      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1541    1827    1881    2271    3028    4466    1541 
   Size:        286      54     390     757    1438    2798

  Fragments arranged by size: 

                2798    1438     757     390     286      54
 
ApaI G_GGCC'C

Cuts at:   2054    2114    2594    2054 
   Size:         60     480    5183

  Fragments arranged by size: 

                5183     480      60
 
ApaLI G'TGCA_C

Cuts at:    627     996    4561     627 
   Size:        369    3565    1789

  Fragments arranged by size: 

                3565    1789     369
 
ApoI r'AATT_y

Cuts at:    943    2727    5076    5332     943 
   Size:       1784    2349     256    1334

  Fragments arranged by size: 

                2349    1784    1334     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5723
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5723
 
AvaI C'yCGr_G

Cuts at:    164    1297    1407    1500    1747    1903    2174    2479    2587 
   Size:       1133     110      93     247     156     271     305     108

Cuts at:   2587    2743     164 
   Size:        156    3144

  Fragments arranged by size: 

                3144    1133     305     271     247     156     156     110
                 108      93
 
BamHI G'GATC_C

Cuts at:   2748    2748 
   Size:       5723
 
BanI G'GyrC_C

Cuts at:    179    1695    2739    3153    3401    3436     179 
   Size:       1516    1044     414     248      35    2466

  Fragments arranged by size: 

                2466    1516    1044     414     248      35
 
BanII G_rGCy'C

Cuts at:    253     758    1298    1904    2054    2114    2175    2594    2703 
   Size:        505     540     606     150      60      61     419     109

Cuts at:   2703    2737    3767     253 
   Size:         34    1030    2209

  Fragments arranged by size: 

                2209    1030     606     540     505     419     150     109
                  61      60      34
 
BbeI G_GCGC'C

Cuts at:   3157    3405    3157 
   Size:        248    5475
 
BbsI GAAGACnn'nnnn_

Cuts at:   1571    1727    1900    1571 
   Size:        156     173    5394

  Fragments arranged by size: 

                5394     173     156
 
BbvCI CC'TCA_GC

Cuts at:   1380    1380 
   Size:       5723
 
BciVI GTATCCnnnnn_n'

Cuts at:   3621    4677    5723    3621 
   Size:       1056    1046    3621

  Fragments arranged by size: 

                3621    1056    1046
 
BclI T'GATC_A

Cuts at:   3243    3243 
   Size:       5723
 
BglI GCCn_nnn'nGGC

Cuts at:   1315    2109    1315 
   Size:        794    4929
 
BglII A'GATC_T

Cuts at:   3238    5275    3238 
   Size:       2037    3686
 
BlpI GC'TnA_GC

Cuts at:    933    1732    2835     933 
   Size:        799    1103    3821

  Fragments arranged by size: 

                3821    1103     799
 
Bme1580I G_kGCm'C

Cuts at:    631    1000    1487    2054    2114    2594    2654    3348    3441 
   Size:        369     487     567      60     480      60     694      93

Cuts at:   3441    4565     631 
   Size:       1124    1789

  Fragments arranged by size: 

                1789    1124     694     567     487     480     369      93
                  60      60
 
BmgBI CAC'GTC

Cuts at:   1161    2187    1161 
   Size:       1026    4697
 
BmrI ACTGGGnnnn_n'

Cuts at:   1104    1511    2013    2520    2935    3016    3351    5495    1104 
   Size:        407     502     507     415      81     335    2144    1332

  Fragments arranged by size: 

                2144    1332     507     502     415     407     335      81
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1395    2707    4090    1395 
   Size:       1312    1383    3028

  Fragments arranged by size: 

                3028    1383    1312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1084    1903    2263    1084 
   Size:        819     360    4544

  Fragments arranged by size: 

                4544     819     360
 
Bpu10I CC'TnA_GC

Cuts at:    841    1380    2272     841 
   Size:        539     892    4292

  Fragments arranged by size: 

                4292     892     539
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    646    2899    3778    4492    4790     646 
   Size:       2253     879     714     298    1579

  Fragments arranged by size: 

                2253    1579     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1060    5427    1060 
   Size:       4367    1356
 
BsaAI yAC'GTr

Cuts at:   1056    3076    1056 
   Size:       2020    3703
 
BsaBI GATnn'nnATC

Cuts at:      6    3237    5274       6 
   Size:       3231    2037     455

  Fragments arranged by size: 

                3231    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1334    3154    3402    4104    5600    1334 
   Size:       1820     248     702    1496    1457

  Fragments arranged by size: 

                1820    1496    1457     702     248
 
BsaWI w'CCGG_w

Cuts at:   1028    2912    3433    4111    4522    4669    4943    1028 
   Size:       1884     521     678     411     147     274    1808

  Fragments arranged by size: 

                1884    1808     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    253     283     296     326     253 
   Size:         30      13      30    5650

  Fragments arranged by size: 

                5650      30      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    162     304     309     544     716     931    1306    1315    1387 
   Size:        142       5     235     172     215     375       9      72

Cuts at:   1387    1449    1765    2683     162 
   Size:         62     316     918    3202

  Fragments arranged by size: 

                3202     918     375     316     235     215     172     142
                  72      62       9       5
 
BseYI C'CCAG_C

Cuts at:   1310    1459    1787    2658    3148    3906    4571    4979    1310 
   Size:        149     328     871     490     758     665     408    2054

  Fragments arranged by size: 

                2054     871     758     665     490     408     328     149
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    675     675 
   Size:       5723
 
BsiEI CG_ry'CG

Cuts at:    104    3265    3311    4541     104 
   Size:       3161      46    1230    1286

  Fragments arranged by size: 

                3161    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    253     631    1000    1910    2737    3515    3705    4565     253 
   Size:        378     369     910     827     778     190     860    1411

  Fragments arranged by size: 

                1411     910     860     827     778     378     369     190
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    190    1325     190 
   Size:       1135    4588
 
Bsp1286I G_dGCh'C

Cuts at:    253     631     758    1000    1298    1487    1904    1910    2054 
   Size:        378     127     242     298     189     417       6     144

Cuts at:   2054    2114    2175    2594    2654    2703    2737    3348    3441 
   Size:         60      61     419      60      49      34     611      93

Cuts at:   3441    3515    3705    3767    4565     253 
   Size:         74     190      62     798    1411

  Fragments arranged by size: 

                1411     798     611     419     417     378     298     242
                 190     189     144     127      93      74      62      61
                  60      60      49      34       6
 
BspHI T'CATG_A

Cuts at:    614    4155     614 
   Size:       3541    2182
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1109    2489    2618    2773    2987    3289    3670    1109 
   Size:       1380     129     155     214     302     381    3162

  Fragments arranged by size: 

                3162    1380     381     302     214     155     129
 
BsrBI CCG'CTC

Cuts at:     50     603     932    1755    2276    4015    4069    5553    5718 
   Size:        553     329     823     521    1739      54    1484     165

Cuts at:   5718      50 
   Size:         55

  Fragments arranged by size: 

                1739    1484     823     553     521     329     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:    401    3635     401 
   Size:       3234    2489
 
BsrFI r'CCGG_y

Cuts at:    182    1910    2579    3721    3902     182 
   Size:       1728     669    1142     181    2003

  Fragments arranged by size: 

                2003    1728    1142     669     181
 
BssSI C'ACGA_G

Cuts at:   3994    4702    3994 
   Size:        708    5015
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1541    3100    1541 
   Size:       1559    4164
 
BstBI TT'CG_AA

Cuts at:   4084    4084 
   Size:       5723
 
BstEII G'GTnAC_C

Cuts at:    411     633     904     411 
   Size:        222     271    5230

  Fragments arranged by size: 

                5230     271     222
 
BstYI r'GATC_y

Cuts at:      1    2008    2031    2748    3215    3238    3573    3819    4125 
   Size:       2007      23     717     467      23     335     246     306

Cuts at:   4125    4137    4223    5275       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                2007    1052     717     467     449     335     306     246
                  86      23      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    914    2335     914 
   Size:       1421    4302
 
BtgI C'CryG_G

Cuts at:    407    1172    1417    1424    1625    1709    1997    2201     407 
   Size:        765     245       7     201      84     288     204    3929

  Fragments arranged by size: 

                3929     765     288     245     204     201      84       7
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3673    3854    4017    3673 
   Size:        181     163    5379

  Fragments arranged by size: 

                5379     181     163
 
BtsI GCAGTG_nn'

Cuts at:    687    1242    1791    2966     687 
   Size:        555     549    1175    3444

  Fragments arranged by size: 

                3444    1175     555     549
 
DraI TTT'AAA

Cuts at:   2722    2722 
   Size:       5723
 
DraIII CAC_nnn'GTG

Cuts at:    246    2060    2293    2562     246 
   Size:       1814     233     269    3407

  Fragments arranged by size: 

                3407    1814     269     233
 
DrdI GACnn_nn'nnGTC

Cuts at:   3429    4773    3429 
   Size:       1344    4379
 
EaeI y'GGCC_r

Cuts at:    927    1020    1211    1971    2198    3308    3482    3873    3900 
   Size:         93     191     760     227    1110     174     391      27

Cuts at:   3900    5245     927 
   Size:       1345    1405

  Fragments arranged by size: 

                1405    1345    1110     760     391     227     191     174
                  93      27
 
EagI C'GGCC_G

Cuts at:   3308    3308 
   Size:       5723
 
EarI CTCTTCn'nnn_

Cuts at:   1255    1624    1998    2246    3746    3956    1255 
   Size:        369     374     248    1500     210    3022

  Fragments arranged by size: 

                3022    1500     374     369     248     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    201    1780    3931    4673    4819    5533     201 
   Size:       1579    2151     742     146     714     391

  Fragments arranged by size: 

                2151    1579     742     714     391     146
 
EcoICRI GAG'CTC

Cuts at:    251    2735     251 
   Size:       2484    3239
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1084    1583    1615    1782    1903    2245    2251    2263    3548 
   Size:        499      32     167     121     342       6      12    1285

Cuts at:   3548    3980    4333    4995    1084 
   Size:        432     353     662    1812

  Fragments arranged by size: 

                1812    1285     662     499     432     353     342     167
                 121      32      12       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2707    2707 
   Size:       5723
 
EcoO109I rG'GnC_Cy

Cuts at:   2110    2111    2268    2283    2421    2591    2688    2862    2110 
   Size:          1     157      15     138     170      97     174    4971

  Fragments arranged by size: 

                4971     174     170     157     138      97      15       1
            
 
EcoRI G'AATT_C

Cuts at:    943    2727     943 
   Size:       1784    3939
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1284    3742    1284 
   Size:       2458    3265
 
FspI TGC'GCA

Cuts at:   3504    3504 
   Size:       5723
 
HaeII r_GCGC'y

Cuts at:   2104    3157    3405    4635    5406    2104 
   Size:       1053     248    1230     771    2421

  Fragments arranged by size: 

                2421    1230    1053     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    240     253     272     285     304     482     514     966     998 
   Size:         13      19      13      19     178      32     452      32

Cuts at:    998    1322    1354    3565    3595    3597    3627     240 
   Size:        324      32    2211      30       2      30    2336

  Fragments arranged by size: 

                2336    2211     452     324     178      32      32      32
                  30      30      19      19      13      13       2
 
HincII GTy'rAC

Cuts at:    657    1128    2762     657 
   Size:        471    1634    3618

  Fragments arranged by size: 

                3618    1634     471
 
KasI G'GCGC_C

Cuts at:   3153    3401    3153 
   Size:        248    5475
 
KpnI G_GTAC'C

Cuts at:   2743    2743 
   Size:       5723
 
MluI A'CGCG_T

Cuts at:   5156    5156 
   Size:       5723
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2240    4482    4666    2240 
   Size:       2242     184    3297

  Fragments arranged by size: 

                3297    2242     184
 
MscI TGG'CCA

Cuts at:   1022    1213    1973    3484    1022 
   Size:        191     760    1511    3261

  Fragments arranged by size: 

                3261    1511     760     191
 
MslI CAynn'nnrTG

Cuts at:    406     619     778     859    3712    3839     406 
   Size:        213     159      81    2853     127    2290

  Fragments arranged by size: 

                2853    2290     213     159     127      81
 
MspA1I CmG'CkG

Cuts at:    115    1314    1495    1541    1881    2241    2451    2834    3148 
   Size:       1199     181      46     340     360     210     383     314

Cuts at:   3148    3508    4292    4537     115 
   Size:        360     784     245    1301

  Fragments arranged by size: 

                1301    1199     784     383     360     360     340     314
                 245     210     181      46
 
NaeI GCC'GGC

Cuts at:    184    2581    3904     184 
   Size:       2397    1323    2003

  Fragments arranged by size: 

                2397    2003    1323
 
NarI GG'CG_CC

Cuts at:   3154    3402    3154 
   Size:        248    5475
 
NcoI C'CATG_G

Cuts at:   1172    1417    1625    1709    1997    2201    1172 
   Size:        245     208      84     288     204    4694

  Fragments arranged by size: 

                4694     288     245     208     204      84
 
NgoMIV G'CCGG_C

Cuts at:    182    2579    3902     182 
   Size:       2397    1323    2003

  Fragments arranged by size: 

                2397    2003    1323
 
NspI r_CATG'y

Cuts at:    594    2776    3276    4879     594 
   Size:       2182     500    1603    1438

  Fragments arranged by size: 

                2182    1603    1438     500
 
PasI CC'CwG_GG

Cuts at:   1071    1071 
   Size:       5723
 
PciI A'CATG_T

Cuts at:    590    4875     590 
   Size:       4285    1438
 
PflMI CCAn_nnn'nTGG

Cuts at:    366     855    1209    1827    2060    2293     366 
   Size:        489     354     618     233     233    3796

  Fragments arranged by size: 

                3796     618     489     354     233     233
 
PfoI T'CCnGG_A

Cuts at:   1859    2264    1859 
   Size:        405    5318
 
PmeI GTTT'AAAC

Cuts at:   2722    2722 
   Size:       5723
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4169    4201    4169 
   Size:         32    5691
 
PpuMI rG'GwC_Cy

Cuts at:   2268    2283    2688    2268 
   Size:         15     405    5303

  Fragments arranged by size: 

                5303     405      15
 
PspOMI G'GGCC_C

Cuts at:   2050    2110    2590    2050 
   Size:         60     480    5183

  Fragments arranged by size: 

                5183     480      60
 
PspXI vC'TCGA_Gb

Cuts at:   2174    2174 
   Size:       5723
 
PstI C_TGCA'G

Cuts at:   1106    2770    3455    1106 
   Size:       1664     685    3374

  Fragments arranged by size: 

                3374    1664     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5723
 
PvuII CAG'CTG

Cuts at:    115    1314    1495    1541    1881    2241    3148    3508     115 
   Size:       1199     181      46     340     360     907     360    2330

  Fragments arranged by size: 

                2330    1199     907     360     360     340     181      46
            
 
RsrII CG'GwC_CG

Cuts at:   3918    3918 
   Size:       5723
 
SacI G_AGCT'C

Cuts at:    253    2737     253 
   Size:       2484    3239
 
SalI G'TCGA_C

Cuts at:   2760    2760 
   Size:       5723
 
SanDI GG'GwC_CC

Cuts at:   2283    2283 
   Size:       5723
 
SapI GCTCTTCn'nnn_

Cuts at:   1624    2246    3746    3956    1624 
   Size:        622    1500     210    3391

  Fragments arranged by size: 

                3391    1500     622     210
 
SbfI CC_TGCA'GG

Cuts at:   2770    2770 
   Size:       5723
 
ScaI AGT'ACT

Cuts at:   5240    5240 
   Size:       5723
 
SexAI A'CCwGG_T

Cuts at:   2289    2289 
   Size:       5723
 
SfcI C'TryA_G

Cuts at:     33    1102    1835    2538    2766    3451    4419    4610      33 
   Size:       1069     733     703     228     685     968     191    1146

  Fragments arranged by size: 

                1146    1069     968     733     703     685     228     191
            
 
SfoI GGC'GCC

Cuts at:   3155    3403    3155 
   Size:        248    5475
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5723
 
SmaI CCC'GGG

Cuts at:    166    1502    2481    2745     166 
   Size:       1336     979     264    3144

  Fragments arranged by size: 

                3144    1336     979     264
 
SmlI C'TyrA_G

Cuts at:    661    2174    2878    3793    4507    4769     661 
   Size:       1513     704     915     714     262    1615

  Fragments arranged by size: 

                1615    1513     915     714     704     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5723
 
SphI G_CATG'C

Cuts at:   2776    3276    2776 
   Size:        500    5223
 
StuI AGG'CCT

Cuts at:    268     268 
   Size:       5723
 
StyI C'CwwG_G

Cuts at:    389    1001    1172    1417    1625    1640    1709    1997    2201 
   Size:        612     171     245     208      15      69     288     204

Cuts at:   2201    2857    3211     389 
   Size:        656     354    2901

  Fragments arranged by size: 

                2901     656     612     354     288     245     208     204
                 171      69      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    815    1462    2419    2539    3306    3439    3898    4107     815 
   Size:        647     957     120     767     133     459     209    2431

  Fragments arranged by size: 

                2431     957     767     647     459     209     133     120
            
 
TatI w'GTAC_w

Cuts at:   5238    5238 
   Size:       5723
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    296     328    1688    1720    5240    5272     296 
   Size:         32    1360      32    3520      32     747

  Fragments arranged by size: 

                3520    1360     747      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2151    3520    2151 
   Size:       1369    4354
 
XbaI T'CTAG_A

Cuts at:   2754    2754 
   Size:       5723
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    279    1344    1456    2421    3218     279 
   Size:       1065     112     965     797    2784

  Fragments arranged by size: 

                2784    1065     965     797     112
 
XhoI C'TCGA_G

Cuts at:   2174    2174 
   Size:       5723
 
XmaI C'CCGG_G

Cuts at:    164    1500    2479    2743     164 
   Size:       1336     979     264    3144

  Fragments arranged by size: 

                3144    1336     979     264
 
XmnI GAAnn'nnTTC

Cuts at:   1618    1618 
   Size:       5723
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AleI     AlfI     AscI
    AvrII     BaeI     BcgI    BfrBI     BmtI    BsiWI     BsmI    BspEI
    BsrGI   BssHII    BstXI  BstZ17I     ClaI    CspCI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmlI    PshAI     PsiI     PsrI    SacII     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     ZraI
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