(Circular) (Six-base) MAPSORT of: pF1KB4384.seq Check: 976 from: 1 to: 5723 pF1KB4384 5723 bp With 182 enzymes: SgfI * June 20, 2008 10:24 .. AccI GT'mk_AC Cuts at: 710 2761 710 Size: 2051 3672 Acc65I G'GTAC_C Cuts at: 2739 2739 Size: 5723 AclI AA'CG_TT Cuts at: 5563 5563 Size: 5723 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1583 1615 1782 2245 2251 3548 3980 4333 4995 Size: 32 167 463 6 1297 432 353 662 Cuts at: 4995 1583 Size: 2311 Fragments arranged by size: 2311 1297 662 463 432 353 167 32 6 AflIII A'CryG_T Cuts at: 590 1055 3075 4875 5156 590 Size: 465 2020 1800 281 1157 Fragments arranged by size: 2020 1800 1157 465 281 AhdI GACnn_n'nnGTC Cuts at: 980 980 Size: 5723 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 850 882 1762 1794 850 Size: 32 880 32 4779 Fragments arranged by size: 4779 880 32 32 AlwNI CAG_nnn'CTG Cuts at: 1541 1827 1881 2271 3028 4466 1541 Size: 286 54 390 757 1438 2798 Fragments arranged by size: 2798 1438 757 390 286 54 ApaI G_GGCC'C Cuts at: 2054 2114 2594 2054 Size: 60 480 5183 Fragments arranged by size: 5183 480 60 ApaLI G'TGCA_C Cuts at: 627 996 4561 627 Size: 369 3565 1789 Fragments arranged by size: 3565 1789 369 ApoI r'AATT_y Cuts at: 943 2727 5076 5332 943 Size: 1784 2349 256 1334 Fragments arranged by size: 2349 1784 1334 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5723 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5723 AvaI C'yCGr_G Cuts at: 164 1297 1407 1500 1747 1903 2174 2479 2587 Size: 1133 110 93 247 156 271 305 108 Cuts at: 2587 2743 164 Size: 156 3144 Fragments arranged by size: 3144 1133 305 271 247 156 156 110 108 93 BamHI G'GATC_C Cuts at: 2748 2748 Size: 5723 BanI G'GyrC_C Cuts at: 179 1695 2739 3153 3401 3436 179 Size: 1516 1044 414 248 35 2466 Fragments arranged by size: 2466 1516 1044 414 248 35 BanII G_rGCy'C Cuts at: 253 758 1298 1904 2054 2114 2175 2594 2703 Size: 505 540 606 150 60 61 419 109 Cuts at: 2703 2737 3767 253 Size: 34 1030 2209 Fragments arranged by size: 2209 1030 606 540 505 419 150 109 61 60 34 BbeI G_GCGC'C Cuts at: 3157 3405 3157 Size: 248 5475 BbsI GAAGACnn'nnnn_ Cuts at: 1571 1727 1900 1571 Size: 156 173 5394 Fragments arranged by size: 5394 173 156 BbvCI CC'TCA_GC Cuts at: 1380 1380 Size: 5723 BciVI GTATCCnnnnn_n' Cuts at: 3621 4677 5723 3621 Size: 1056 1046 3621 Fragments arranged by size: 3621 1056 1046 BclI T'GATC_A Cuts at: 3243 3243 Size: 5723 BglI GCCn_nnn'nGGC Cuts at: 1315 2109 1315 Size: 794 4929 BglII A'GATC_T Cuts at: 3238 5275 3238 Size: 2037 3686 BlpI GC'TnA_GC Cuts at: 933 1732 2835 933 Size: 799 1103 3821 Fragments arranged by size: 3821 1103 799 Bme1580I G_kGCm'C Cuts at: 631 1000 1487 2054 2114 2594 2654 3348 3441 Size: 369 487 567 60 480 60 694 93 Cuts at: 3441 4565 631 Size: 1124 1789 Fragments arranged by size: 1789 1124 694 567 487 480 369 93 60 60 BmgBI CAC'GTC Cuts at: 1161 2187 1161 Size: 1026 4697 BmrI ACTGGGnnnn_n' Cuts at: 1104 1511 2013 2520 2935 3016 3351 5495 1104 Size: 407 502 507 415 81 335 2144 1332 Fragments arranged by size: 2144 1332 507 502 415 407 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1395 2707 4090 1395 Size: 1312 1383 3028 Fragments arranged by size: 3028 1383 1312 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1084 1903 2263 1084 Size: 819 360 4544 Fragments arranged by size: 4544 819 360 Bpu10I CC'TnA_GC Cuts at: 841 1380 2272 841 Size: 539 892 4292 Fragments arranged by size: 4292 892 539 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 646 2899 3778 4492 4790 646 Size: 2253 879 714 298 1579 Fragments arranged by size: 2253 1579 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 1060 5427 1060 Size: 4367 1356 BsaAI yAC'GTr Cuts at: 1056 3076 1056 Size: 2020 3703 BsaBI GATnn'nnATC Cuts at: 6 3237 5274 6 Size: 3231 2037 455 Fragments arranged by size: 3231 2037 455 BsaHI Gr'CG_yC Cuts at: 1334 3154 3402 4104 5600 1334 Size: 1820 248 702 1496 1457 Fragments arranged by size: 1820 1496 1457 702 248 BsaWI w'CCGG_w Cuts at: 1028 2912 3433 4111 4522 4669 4943 1028 Size: 1884 521 678 411 147 274 1808 Fragments arranged by size: 1884 1808 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 253 283 296 326 253 Size: 30 13 30 5650 Fragments arranged by size: 5650 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 162 304 309 544 716 931 1306 1315 1387 Size: 142 5 235 172 215 375 9 72 Cuts at: 1387 1449 1765 2683 162 Size: 62 316 918 3202 Fragments arranged by size: 3202 918 375 316 235 215 172 142 72 62 9 5 BseYI C'CCAG_C Cuts at: 1310 1459 1787 2658 3148 3906 4571 4979 1310 Size: 149 328 871 490 758 665 408 2054 Fragments arranged by size: 2054 871 758 665 490 408 328 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 675 675 Size: 5723 BsiEI CG_ry'CG Cuts at: 104 3265 3311 4541 104 Size: 3161 46 1230 1286 Fragments arranged by size: 3161 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 253 631 1000 1910 2737 3515 3705 4565 253 Size: 378 369 910 827 778 190 860 1411 Fragments arranged by size: 1411 910 860 827 778 378 369 190 BsmBI CGTCTCn'nnnn_ Cuts at: 190 1325 190 Size: 1135 4588 Bsp1286I G_dGCh'C Cuts at: 253 631 758 1000 1298 1487 1904 1910 2054 Size: 378 127 242 298 189 417 6 144 Cuts at: 2054 2114 2175 2594 2654 2703 2737 3348 3441 Size: 60 61 419 60 49 34 611 93 Cuts at: 3441 3515 3705 3767 4565 253 Size: 74 190 62 798 1411 Fragments arranged by size: 1411 798 611 419 417 378 298 242 190 189 144 127 93 74 62 61 60 60 49 34 6 BspHI T'CATG_A Cuts at: 614 4155 614 Size: 3541 2182 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1109 2489 2618 2773 2987 3289 3670 1109 Size: 1380 129 155 214 302 381 3162 Fragments arranged by size: 3162 1380 381 302 214 155 129 BsrBI CCG'CTC Cuts at: 50 603 932 1755 2276 4015 4069 5553 5718 Size: 553 329 823 521 1739 54 1484 165 Cuts at: 5718 50 Size: 55 Fragments arranged by size: 1739 1484 823 553 521 329 165 55 54 BsrDI GCAATG_nn' Cuts at: 401 3635 401 Size: 3234 2489 BsrFI r'CCGG_y Cuts at: 182 1910 2579 3721 3902 182 Size: 1728 669 1142 181 2003 Fragments arranged by size: 2003 1728 1142 669 181 BssSI C'ACGA_G Cuts at: 3994 4702 3994 Size: 708 5015 BstAPI GCAn_nnn'nTGC Cuts at: 1541 3100 1541 Size: 1559 4164 BstBI TT'CG_AA Cuts at: 4084 4084 Size: 5723 BstEII G'GTnAC_C Cuts at: 411 633 904 411 Size: 222 271 5230 Fragments arranged by size: 5230 271 222 BstYI r'GATC_y Cuts at: 1 2008 2031 2748 3215 3238 3573 3819 4125 Size: 2007 23 717 467 23 335 246 306 Cuts at: 4125 4137 4223 5275 1 Size: 12 86 1052 449 Fragments arranged by size: 2007 1052 717 467 449 335 306 246 86 23 23 12 Bsu36I CC'TnA_GG Cuts at: 914 2335 914 Size: 1421 4302 BtgI C'CryG_G Cuts at: 407 1172 1417 1424 1625 1709 1997 2201 407 Size: 765 245 7 201 84 288 204 3929 Fragments arranged by size: 3929 765 288 245 204 201 84 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3673 3854 4017 3673 Size: 181 163 5379 Fragments arranged by size: 5379 181 163 BtsI GCAGTG_nn' Cuts at: 687 1242 1791 2966 687 Size: 555 549 1175 3444 Fragments arranged by size: 3444 1175 555 549 DraI TTT'AAA Cuts at: 2722 2722 Size: 5723 DraIII CAC_nnn'GTG Cuts at: 246 2060 2293 2562 246 Size: 1814 233 269 3407 Fragments arranged by size: 3407 1814 269 233 DrdI GACnn_nn'nnGTC Cuts at: 3429 4773 3429 Size: 1344 4379 EaeI y'GGCC_r Cuts at: 927 1020 1211 1971 2198 3308 3482 3873 3900 Size: 93 191 760 227 1110 174 391 27 Cuts at: 3900 5245 927 Size: 1345 1405 Fragments arranged by size: 1405 1345 1110 760 391 227 191 174 93 27 EagI C'GGCC_G Cuts at: 3308 3308 Size: 5723 EarI CTCTTCn'nnn_ Cuts at: 1255 1624 1998 2246 3746 3956 1255 Size: 369 374 248 1500 210 3022 Fragments arranged by size: 3022 1500 374 369 248 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 201 1780 3931 4673 4819 5533 201 Size: 1579 2151 742 146 714 391 Fragments arranged by size: 2151 1579 742 714 391 146 EcoICRI GAG'CTC Cuts at: 251 2735 251 Size: 2484 3239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1084 1583 1615 1782 1903 2245 2251 2263 3548 Size: 499 32 167 121 342 6 12 1285 Cuts at: 3548 3980 4333 4995 1084 Size: 432 353 662 1812 Fragments arranged by size: 1812 1285 662 499 432 353 342 167 121 32 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2707 2707 Size: 5723 EcoO109I rG'GnC_Cy Cuts at: 2110 2111 2268 2283 2421 2591 2688 2862 2110 Size: 1 157 15 138 170 97 174 4971 Fragments arranged by size: 4971 174 170 157 138 97 15 1 EcoRI G'AATT_C Cuts at: 943 2727 943 Size: 1784 3939 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1284 3742 1284 Size: 2458 3265 FspI TGC'GCA Cuts at: 3504 3504 Size: 5723 HaeII r_GCGC'y Cuts at: 2104 3157 3405 4635 5406 2104 Size: 1053 248 1230 771 2421 Fragments arranged by size: 2421 1230 1053 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 240 253 272 285 304 482 514 966 998 Size: 13 19 13 19 178 32 452 32 Cuts at: 998 1322 1354 3565 3595 3597 3627 240 Size: 324 32 2211 30 2 30 2336 Fragments arranged by size: 2336 2211 452 324 178 32 32 32 30 30 19 19 13 13 2 HincII GTy'rAC Cuts at: 657 1128 2762 657 Size: 471 1634 3618 Fragments arranged by size: 3618 1634 471 KasI G'GCGC_C Cuts at: 3153 3401 3153 Size: 248 5475 KpnI G_GTAC'C Cuts at: 2743 2743 Size: 5723 MluI A'CGCG_T Cuts at: 5156 5156 Size: 5723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2240 4482 4666 2240 Size: 2242 184 3297 Fragments arranged by size: 3297 2242 184 MscI TGG'CCA Cuts at: 1022 1213 1973 3484 1022 Size: 191 760 1511 3261 Fragments arranged by size: 3261 1511 760 191 MslI CAynn'nnrTG Cuts at: 406 619 778 859 3712 3839 406 Size: 213 159 81 2853 127 2290 Fragments arranged by size: 2853 2290 213 159 127 81 MspA1I CmG'CkG Cuts at: 115 1314 1495 1541 1881 2241 2451 2834 3148 Size: 1199 181 46 340 360 210 383 314 Cuts at: 3148 3508 4292 4537 115 Size: 360 784 245 1301 Fragments arranged by size: 1301 1199 784 383 360 360 340 314 245 210 181 46 NaeI GCC'GGC Cuts at: 184 2581 3904 184 Size: 2397 1323 2003 Fragments arranged by size: 2397 2003 1323 NarI GG'CG_CC Cuts at: 3154 3402 3154 Size: 248 5475 NcoI C'CATG_G Cuts at: 1172 1417 1625 1709 1997 2201 1172 Size: 245 208 84 288 204 4694 Fragments arranged by size: 4694 288 245 208 204 84 NgoMIV G'CCGG_C Cuts at: 182 2579 3902 182 Size: 2397 1323 2003 Fragments arranged by size: 2397 2003 1323 NspI r_CATG'y Cuts at: 594 2776 3276 4879 594 Size: 2182 500 1603 1438 Fragments arranged by size: 2182 1603 1438 500 PasI CC'CwG_GG Cuts at: 1071 1071 Size: 5723 PciI A'CATG_T Cuts at: 590 4875 590 Size: 4285 1438 PflMI CCAn_nnn'nTGG Cuts at: 366 855 1209 1827 2060 2293 366 Size: 489 354 618 233 233 3796 Fragments arranged by size: 3796 618 489 354 233 233 PfoI T'CCnGG_A Cuts at: 1859 2264 1859 Size: 405 5318 PmeI GTTT'AAAC Cuts at: 2722 2722 Size: 5723 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 4169 Size: 32 5691 PpuMI rG'GwC_Cy Cuts at: 2268 2283 2688 2268 Size: 15 405 5303 Fragments arranged by size: 5303 405 15 PspOMI G'GGCC_C Cuts at: 2050 2110 2590 2050 Size: 60 480 5183 Fragments arranged by size: 5183 480 60 PspXI vC'TCGA_Gb Cuts at: 2174 2174 Size: 5723 PstI C_TGCA'G Cuts at: 1106 2770 3455 1106 Size: 1664 685 3374 Fragments arranged by size: 3374 1664 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5723 PvuII CAG'CTG Cuts at: 115 1314 1495 1541 1881 2241 3148 3508 115 Size: 1199 181 46 340 360 907 360 2330 Fragments arranged by size: 2330 1199 907 360 360 340 181 46 RsrII CG'GwC_CG Cuts at: 3918 3918 Size: 5723 SacI G_AGCT'C Cuts at: 253 2737 253 Size: 2484 3239 SalI G'TCGA_C Cuts at: 2760 2760 Size: 5723 SanDI GG'GwC_CC Cuts at: 2283 2283 Size: 5723 SapI GCTCTTCn'nnn_ Cuts at: 1624 2246 3746 3956 1624 Size: 622 1500 210 3391 Fragments arranged by size: 3391 1500 622 210 SbfI CC_TGCA'GG Cuts at: 2770 2770 Size: 5723 ScaI AGT'ACT Cuts at: 5240 5240 Size: 5723 SexAI A'CCwGG_T Cuts at: 2289 2289 Size: 5723 SfcI C'TryA_G Cuts at: 33 1102 1835 2538 2766 3451 4419 4610 33 Size: 1069 733 703 228 685 968 191 1146 Fragments arranged by size: 1146 1069 968 733 703 685 228 191 SfoI GGC'GCC Cuts at: 3155 3403 3155 Size: 248 5475 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5723 SmaI CCC'GGG Cuts at: 166 1502 2481 2745 166 Size: 1336 979 264 3144 Fragments arranged by size: 3144 1336 979 264 SmlI C'TyrA_G Cuts at: 661 2174 2878 3793 4507 4769 661 Size: 1513 704 915 714 262 1615 Fragments arranged by size: 1615 1513 915 714 704 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5723 SphI G_CATG'C Cuts at: 2776 3276 2776 Size: 500 5223 StuI AGG'CCT Cuts at: 268 268 Size: 5723 StyI C'CwwG_G Cuts at: 389 1001 1172 1417 1625 1640 1709 1997 2201 Size: 612 171 245 208 15 69 288 204 Cuts at: 2201 2857 3211 389 Size: 656 354 2901 Fragments arranged by size: 2901 656 612 354 288 245 208 204 171 69 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 815 1462 2419 2539 3306 3439 3898 4107 815 Size: 647 957 120 767 133 459 209 2431 Fragments arranged by size: 2431 957 767 647 459 209 133 120 TatI w'GTAC_w Cuts at: 5238 5238 Size: 5723 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 296 328 1688 1720 5240 5272 296 Size: 32 1360 32 3520 32 747 Fragments arranged by size: 3520 1360 747 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2151 3520 2151 Size: 1369 4354 XbaI T'CTAG_A Cuts at: 2754 2754 Size: 5723 XcmI CCAnnnn_n'nnnnTGG Cuts at: 279 1344 1456 2421 3218 279 Size: 1065 112 965 797 2784 Fragments arranged by size: 2784 1065 965 797 112 XhoI C'TCGA_G Cuts at: 2174 2174 Size: 5723 XmaI C'CCGG_G Cuts at: 164 1500 2479 2743 164 Size: 1336 979 264 3144 Fragments arranged by size: 3144 1336 979 264 XmnI GAAnn'nnTTC Cuts at: 1618 1618 Size: 5723 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AleI AlfI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BspEI BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI ZraI