Restriction map for
pF1KB4387
(Circular) (Six-base) MAPSORT of: pF1KB4387.seq Check: 1789 from: 1 to: 5687
pF1KB4387 5687 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 316 592 1204 316
Size: 276 612 4799
Fragments arranged by size:
4799 612 276
AccI GT'mk_AC
Cuts at: 2725 2725
Size: 5687
Acc65I G'GTAC_C
Cuts at: 2703 2703
Size: 5687
AclI AA'CG_TT
Cuts at: 1636 5527 1636
Size: 3891 1796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 3512 3944 4297 4959 309
Size: 3203 432 353 662 1037
Fragments arranged by size:
3203 1037 662 432 353
AfeI AGC'GCT
Cuts at: 255 655 255
Size: 400 5287
AflII C'TTAA_G
Cuts at: 1102 2551 1102
Size: 1449 4238
AflIII A'CryG_T
Cuts at: 671 3039 4839 5120 671
Size: 2368 1800 281 1238
Fragments arranged by size:
2368 1800 1238 281
AleI CACnn'nnGTG
Cuts at: 1411 2389 1411
Size: 978 4709
AlwNI CAG_nnn'CTG
Cuts at: 2145 2251 2992 4430 2145
Size: 106 741 1438 3402
Fragments arranged by size:
3402 1438 741 106
ApaI G_GGCC'C
Cuts at: 116 116
Size: 5687
ApaLI G'TGCA_C
Cuts at: 4525 4525
Size: 5687
ApoI r'AATT_y
Cuts at: 826 2691 5040 5296 826
Size: 1865 2349 256 1217
Fragments arranged by size:
2349 1865 1217 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
AvaI C'yCGr_G
Cuts at: 2707 2707
Size: 5687
BamHI G'GATC_C
Cuts at: 1185 1556 1680 2712 1185
Size: 371 124 1032 4160
Fragments arranged by size:
4160 1032 371 124
BanI G'GyrC_C
Cuts at: 2703 3117 3365 3400 2703
Size: 414 248 35 4990
Fragments arranged by size:
4990 414 248 35
BanII G_rGCy'C
Cuts at: 116 1297 1485 2384 2475 2701 3731 116
Size: 1181 188 899 91 226 1030 2072
Fragments arranged by size:
2072 1181 1030 899 226 188 91
BbeI G_GCGC'C
Cuts at: 3121 3369 3121
Size: 248 5439
BbsI GAAGACnn'nnnn_
Cuts at: 1900 2499 2517 2602 1900
Size: 599 18 85 4985
Fragments arranged by size:
4985 599 85 18
BbvCI CC'TCA_GC
Cuts at: 2252 2252
Size: 5687
BciVI GTATCCnnnnn_n'
Cuts at: 1605 3585 4641 5687 1605
Size: 1980 1056 1046 1605
Fragments arranged by size:
1980 1605 1056 1046
BclI T'GATC_A
Cuts at: 3207 3207
Size: 5687
BfrBI ATG'CAT
Cuts at: 224 974 224
Size: 750 4937
BglI GCCn_nnn'nGGC
Cuts at: 269 725 1133 1285 2207 269
Size: 456 408 152 922 3749
Fragments arranged by size:
3749 922 456 408 152
BglII A'GATC_T
Cuts at: 1812 3202 5239 1812
Size: 1390 2037 2260
Fragments arranged by size:
2260 2037 1390
BlpI GC'TnA_GC
Cuts at: 2799 2799
Size: 5687
Bme1580I G_kGCm'C
Cuts at: 116 1129 1597 3312 3405 4529 116
Size: 1013 468 1715 93 1124 1274
Fragments arranged by size:
1715 1274 1124 1013 468 93
BmgBI CAC'GTC
Cuts at: 293 431 1089 2676 293
Size: 138 658 1587 3304
Fragments arranged by size:
3304 1587 658 138
BmrI ACTGGGnnnn_n'
Cuts at: 1722 1845 2453 2899 2980 3315 5459 1722
Size: 123 608 446 81 335 2144 1950
Fragments arranged by size:
2144 1950 608 446 335 123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1671 4054 1671
Size: 2383 3304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 726 1240 2686 387
Size: 339 514 1446 3388
Fragments arranged by size:
3388 1446 514 339
Bpu10I CC'TnA_GC
Cuts at: 2122 2252 2122
Size: 130 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1848 2863 3742 4456 4754 1848
Size: 1015 879 714 298 2781
Fragments arranged by size:
2781 1015 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5391 5391
Size: 5687
BsaAI yAC'GTr
Cuts at: 672 3040 672
Size: 2368 3319
BsaBI GATnn'nnATC
Cuts at: 6 3201 5238 6
Size: 3195 2037 455
Fragments arranged by size:
3195 2037 455
BsaHI Gr'CG_yC
Cuts at: 1490 3118 3366 4068 5564 1490
Size: 1628 248 702 1496 1613
Fragments arranged by size:
1628 1613 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1889 2876 3397 4075 4486 4633 4907 1352
Size: 537 987 521 678 411 147 274 2132
Fragments arranged by size:
2132 987 678 537 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 514 544 1918 1948 514
Size: 30 1374 30 4253
Fragments arranged by size:
4253 1374 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 1807 2108 2247 2491 142
Size: 1665 301 139 244 3338
Fragments arranged by size:
3338 1665 301 244 139
BseYI C'CCAG_C
Cuts at: 593 1151 1469 2084 3112 3870 4535 4943 593
Size: 558 318 615 1028 758 665 408 1337
Fragments arranged by size:
1337 1028 758 665 615 558 408 318
BsiEI CG_ry'CG
Cuts at: 104 3229 3275 4505 104
Size: 3125 46 1230 1286
Fragments arranged by size:
3125 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 436 545 838 2501 2701 3479 3669 4529 436
Size: 109 293 1663 200 778 190 860 1594
Fragments arranged by size:
1663 1594 860 778 293 200 190 109
BsmI GAATG_Cn'
Cuts at: 353 976 1063 353
Size: 623 87 4977
Fragments arranged by size:
4977 623 87
BsmBI CGTCTCn'nnnn_
Cuts at: 283 1497 283
Size: 1214 4473
Bsp1286I G_dGCh'C
Cuts at: 116 436 545 838 1129 1297 1485 1597 2384
Size: 320 109 293 291 168 188 112 787
Cuts at: 2384 2475 2501 2701 3312 3405 3479 3669 3731
Size: 91 26 200 611 93 74 190 62
Cuts at: 3731 4529 116
Size: 798 1274
Fragments arranged by size:
1274 798 787 611 320 293 291 200
190 188 168 112 109 93 91 74
62 26
BspHI T'CATG_A
Cuts at: 4119 4119
Size: 5687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 316 592 1204 2200 2737 2951 3253 3634 316
Size: 276 612 996 537 214 302 381 2369
Fragments arranged by size:
2369 996 612 537 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 3979 4033 5517 5682 50
Size: 3929 54 1484 165 55
Fragments arranged by size:
3929 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1101 2133 3599 1101
Size: 1032 1466 3189
Fragments arranged by size:
3189 1466 1032
BsrFI r'CCGG_y
Cuts at: 863 1322 3685 3866 863
Size: 459 2363 181 2684
Fragments arranged by size:
2684 2363 459 181
BssSI C'ACGA_G
Cuts at: 1327 3958 4666 1327
Size: 2631 708 2348
Fragments arranged by size:
2631 2348 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3064 3064
Size: 5687
BstBI TT'CG_AA
Cuts at: 4048 4048
Size: 5687
BstXI CCAn_nnnn'nTGG
Cuts at: 1677 1677
Size: 5687
BstYI r'GATC_y
Cuts at: 1 1185 1556 1680 1812 2712 3179 3202 3537
Size: 1184 371 124 132 900 467 23 335
Cuts at: 3537 3783 4089 4101 4187 5239 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1184 1052 900 467 449 371 335 306
246 132 124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1384 1384
Size: 5687
BtgI C'CryG_G
Cuts at: 107 158 855 1485 2387 107
Size: 51 697 630 902 3407
Fragments arranged by size:
3407 902 697 630 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 260 389 917 1284 3637 3818 3981 260
Size: 129 528 367 2353 181 163 1966
Fragments arranged by size:
2353 1966 528 367 181 163 129
BtsI GCAGTG_nn'
Cuts at: 179 714 874 2267 2930 179
Size: 535 160 1393 663 2936
Fragments arranged by size:
2936 1393 663 535 160
ClaI AT'CG_AT
Cuts at: 1172 1871 1172
Size: 699 4988
DraI TTT'AAA
Cuts at: 2686 2686
Size: 5687
DrdI GACnn_nn'nnGTC
Cuts at: 297 2584 3393 4737 297
Size: 2287 809 1344 1247
Fragments arranged by size:
2287 1344 1247 809
EaeI y'GGCC_r
Cuts at: 378 646 806 1415 1824 1940 2219 2264 3272
Size: 268 160 609 409 116 279 45 1008
Cuts at: 3272 3446 3837 3864 5209 378
Size: 174 391 27 1345 856
Fragments arranged by size:
1345 1008 856 609 409 391 279 268
174 160 116 45 27
EagI C'GGCC_G
Cuts at: 3272 3272
Size: 5687
EarI CTCTTCn'nnn_
Cuts at: 1315 1500 1621 2074 3710 3920 1315
Size: 185 121 453 1636 210 3082
Fragments arranged by size:
3082 1636 453 210 185 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3895 4637 4783 5497 3895
Size: 742 146 714 4085
Fragments arranged by size:
4085 742 714 146
EcoICRI GAG'CTC
Cuts at: 2699 2699
Size: 5687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 387 726 1240 2686 3512 3944 4297 4959
Size: 78 339 514 1446 826 432 353 662
Cuts at: 4959 309
Size: 1037
Fragments arranged by size:
1446 1037 826 662 514 432 353 339
78
EcoNI CCTnn'n_nnAGG
Cuts at: 2245 2245
Size: 5687
EcoO109I rG'GnC_Cy
Cuts at: 112 113 940 1530 1743 2826 112
Size: 1 827 590 213 1083 2973
Fragments arranged by size:
2973 1083 827 590 213 1
EcoRI G'AATT_C
Cuts at: 826 2691 826
Size: 1865 3822
EcoRV GAT'ATC
Cuts at: 1170 1974 1170
Size: 804 4883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1917 3706 1917
Size: 1789 3898
FspI TGC'GCA
Cuts at: 3468 3468
Size: 5687
HaeII r_GCGC'y
Cuts at: 257 657 753 3121 3369 4599 5370 257
Size: 400 96 2368 248 1230 771 574
Fragments arranged by size:
2368 1230 771 574 400 248 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 514 546 1750 1782 2394 2426 2646
Size: 32 97 32 1204 32 612 32 220
Cuts at: 2646 2678 3529 3559 3561 3591 385
Size: 32 851 30 2 30 2481
Fragments arranged by size:
2481 1204 851 612 220 97 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 345 1842 1878 2025 2726 345
Size: 1497 36 147 701 3306
Fragments arranged by size:
3306 1497 701 147 36
KasI G'GCGC_C
Cuts at: 3117 3365 3117
Size: 248 5439
KpnI G_GTAC'C
Cuts at: 2707 2707
Size: 5687
MluI A'CGCG_T
Cuts at: 5120 5120
Size: 5687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4446 4630 4446
Size: 184 5503
MscI TGG'CCA
Cuts at: 380 808 1417 2221 2266 3448 380
Size: 428 609 804 45 1182 2619
Fragments arranged by size:
2619 1182 804 609 428 45
MslI CAynn'nnrTG
Cuts at: 1411 1675 2389 3676 3803 1411
Size: 264 714 1287 127 3295
Fragments arranged by size:
3295 1287 714 264 127
MspA1I CmG'CkG
Cuts at: 160 329 350 597 746 965 1143 1260 2798
Size: 169 21 247 149 219 178 117 1538
Cuts at: 2798 3112 3472 4256 4501 160
Size: 314 360 784 245 1346
Fragments arranged by size:
1538 1346 784 360 314 247 245 219
178 169 149 117 21
NaeI GCC'GGC
Cuts at: 865 3868 865
Size: 3003 2684
NarI GG'CG_CC
Cuts at: 3118 3366 3118
Size: 248 5439
NcoI C'CATG_G
Cuts at: 107 855 107
Size: 748 4939
NgoMIV G'CCGG_C
Cuts at: 863 3866 863
Size: 3003 2684
NsiI A_TGCA'T
Cuts at: 226 976 226
Size: 750 4937
NspI r_CATG'y
Cuts at: 2214 2740 3240 4843 2214
Size: 526 500 1603 3058
Fragments arranged by size:
3058 1603 526 500
PasI CC'CwG_GG
Cuts at: 116 2377 116
Size: 2261 3426
PciI A'CATG_T
Cuts at: 4839 4839
Size: 5687
PflMI CCAn_nnn'nTGG
Cuts at: 1146 2421 1146
Size: 1275 4412
PmeI GTTT'AAAC
Cuts at: 2686 2686
Size: 5687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 4133
Size: 32 5655
PpuMI rG'GwC_Cy
Cuts at: 940 940
Size: 5687
PspOMI G'GGCC_C
Cuts at: 112 112
Size: 5687
PstI C_TGCA'G
Cuts at: 174 1201 1465 2262 2734 3419 174
Size: 1027 264 797 472 685 2442
Fragments arranged by size:
2442 1027 797 685 472 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5687
PvuII CAG'CTG
Cuts at: 329 597 746 1143 1260 3112 3472 329
Size: 268 149 397 117 1852 360 2544
Fragments arranged by size:
2544 1852 397 360 268 149 117
RsrII CG'GwC_CG
Cuts at: 3882 3882
Size: 5687
SacI G_AGCT'C
Cuts at: 2701 2701
Size: 5687
SacII CC_GC'GG
Cuts at: 161 161
Size: 5687
SalI G'TCGA_C
Cuts at: 2724 2724
Size: 5687
SanDI GG'GwC_CC
Cuts at: 940 940
Size: 5687
SapI GCTCTTCn'nnn_
Cuts at: 3710 3920 3710
Size: 210 5477
SbfI CC_TGCA'GG
Cuts at: 2734 2734
Size: 5687
ScaI AGT'ACT
Cuts at: 5204 5204
Size: 5687
SfcI C'TryA_G
Cuts at: 33 170 657 1197 1461 2258 2640 2730 3415
Size: 137 487 540 264 797 382 90 685
Cuts at: 3415 4383 4574 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 797 685 540 487 382 264
191 137 90
SfoI GGC'GCC
Cuts at: 3119 3367 3119
Size: 248 5439
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5687
SmaI CCC'GGG
Cuts at: 2709 2709
Size: 5687
SmlI C'TyrA_G
Cuts at: 1102 1863 2551 2842 3757 4471 4733 1102
Size: 761 688 291 915 714 262 2056
Fragments arranged by size:
2056 915 761 714 688 291 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5687
SphI G_CATG'C
Cuts at: 2214 2740 3240 2214
Size: 526 500 4661
Fragments arranged by size:
4661 526 500
StuI AGG'CCT
Cuts at: 529 1279 529
Size: 750 4937
StyI C'CwwG_G
Cuts at: 107 678 855 1616 1663 1937 2821 3175 107
Size: 571 177 761 47 274 884 354 2619
Fragments arranged by size:
2619 884 761 571 354 274 177 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1483 1594 2098 2284 3270 3403 3862 4071 1483
Size: 111 504 186 986 133 459 209 3099
Fragments arranged by size:
3099 986 504 459 209 186 133 111
TatI w'GTAC_w
Cuts at: 1361 1700 5202 1361
Size: 339 3502 1846
Fragments arranged by size:
3502 1846 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 537 569 1918 1950 5204 5236 537
Size: 32 1349 32 3254 32 988
Fragments arranged by size:
3254 1349 988 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 988 1507 1585 2406 3484 988
Size: 519 78 821 1078 3191
Fragments arranged by size:
3191 1078 821 519 78
XbaI T'CTAG_A
Cuts at: 2718 2718
Size: 5687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 721 838 1059 3182 721
Size: 117 221 2123 3226
Fragments arranged by size:
3226 2123 221 117
XmaI C'CCGG_G
Cuts at: 2707 2707
Size: 5687
XmnI GAAnn'nnTTC
Cuts at: 1784 1784
Size: 5687
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AhdI AlfI AloI AscI AvrII BaeI
BcgI BmtI BsgI BsiWI BspEI BsrGI BssHII BstEII
BstZ17I CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI