Restriction map for
pF1KB4388
(Circular) (Six-base) MAPSORT of: pF1KB4388.seq Check: 7314 from: 1 to: 4160
pF1KB4388 4160 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1198 1198
Size: 4160
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 786 1985 2417 2770 3432 463
Size: 323 1199 432 353 662 1191
Fragments arranged by size:
1199 1191 662 432 353 323
AfeI AGC'GCT
Cuts at: 853 853
Size: 4160
AflIII A'CryG_T
Cuts at: 1512 3312 3593 1512
Size: 1800 281 2079
Fragments arranged by size:
2079 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1001 1465 2903 1001
Size: 464 1438 2258
Fragments arranged by size:
2258 1438 464
ApaLI G'TGCA_C
Cuts at: 272 2998 272
Size: 2726 1434
ApoI r'AATT_y
Cuts at: 1164 3513 3769 1164
Size: 2349 256 1555
Fragments arranged by size:
2349 1555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 1180 1180
Size: 4160
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 1174 2204 1174
Size: 1030 3130
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 579 579
Size: 4160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 939 973 939
Size: 34 4126
BciVI GTATCCnnnnn_n'
Cuts at: 2058 3114 4160 2058
Size: 1056 1046 2058
Fragments arranged by size:
2058 1056 1046
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 276 1785 1878 3002 276
Size: 1509 93 1124 1434
Fragments arranged by size:
1509 1434 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 618 833 1372 1453 1788 3932 618
Size: 215 539 81 335 2144 846
Fragments arranged by size:
2144 846 539 335 215 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 558
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2929 3227 1336
Size: 879 714 298 2269
Fragments arranged by size:
2269 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 128 1674 3711 6
Size: 122 1546 2037 455
Fragments arranged by size:
2037 1546 455 122
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1870 2548 2959 3106 3380 1349
Size: 521 678 411 147 274 2129
Fragments arranged by size:
2129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 655
Size: 30 4130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 412 364
Size: 48 4112
BseYI C'CCAG_C
Cuts at: 711 1585 2343 3008 3416 711
Size: 874 758 665 408 1455
Fragments arranged by size:
1455 874 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 853
Size: 4160
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2978 104
Size: 1598 46 1230 1286
Fragments arranged by size:
1598 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 276 1174 1952 2142 3002 276
Size: 898 778 190 860 1434
Fragments arranged by size:
1434 898 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 456 456
Size: 4160
Bsp1286I G_dGCh'C
Cuts at: 276 1174 1785 1878 1952 2142 2204 3002 276
Size: 898 611 93 74 190 62 798 1434
Fragments arranged by size:
1434 898 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2592 2592
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1424 1726 2107 1210
Size: 214 302 381 3263
Fragments arranged by size:
3263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 491 2072 491
Size: 1581 2579
BsrFI r'CCGG_y
Cuts at: 2158 2339 2158
Size: 181 3979
BssSI C'ACGA_G
Cuts at: 2431 3139 2431
Size: 708 3452
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 873 2521 873
Size: 1648 2512
BstXI CCAn_nnnn'nTGG
Cuts at: 421 421
Size: 4160
BstYI r'GATC_y
Cuts at: 1 1185 1652 1675 2010 2256 2562 2574 2660
Size: 1184 467 23 335 246 306 12 86
Cuts at: 2660 3712 1
Size: 1052 449
Fragments arranged by size:
1184 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 455 860 455
Size: 405 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 130 165 963 998 130
Size: 35 798 35 3292
Fragments arranged by size:
3292 798 35 35
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 3210 1866
Size: 1344 2816
EaeI y'GGCC_r
Cuts at: 942 1745 1919 2310 2337 3682 942
Size: 803 174 391 27 1345 1420
Fragments arranged by size:
1420 1345 803 391 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 439 2183 2393 439
Size: 1744 210 2206
Fragments arranged by size:
2206 1744 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 3110 3256 3970 2368
Size: 742 146 714 2558
Fragments arranged by size:
2558 742 714 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 558 786 1985 2417 2770 3432 463
Size: 95 228 1199 432 353 662 1191
Fragments arranged by size:
1199 1191 662 432 353 228 95
EcoO109I rG'GnC_Cy
Cuts at: 1132 1299 1132
Size: 167 3993
EcoRI G'AATT_C
Cuts at: 1164 1164
Size: 4160
EcoRV GAT'ATC
Cuts at: 625 780 625
Size: 155 4005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1153 2179 1153
Size: 1026 3134
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 855 1059 1594 1842 3072 3843 855
Size: 204 535 248 1230 771 1172
Fragments arranged by size:
1230 1172 771 535 248 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1136 1168 2002 2032 2034 2064 1136
Size: 32 834 30 2 30 3232
Fragments arranged by size:
3232 834 32 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 908 1052 2919 3103 908
Size: 144 1867 184 1965
Fragments arranged by size:
1965 1867 184 144
MscI TGG'CCA
Cuts at: 944 1921 944
Size: 977 3183
MslI CAynn'nnrTG
Cuts at: 250 632 694 2149 2276 250
Size: 382 62 1455 127 2134
Fragments arranged by size:
2134 1455 382 127 62
MspA1I CmG'CkG
Cuts at: 1271 1585 1945 2729 2974 1271
Size: 314 360 784 245 2457
Fragments arranged by size:
2457 784 360 314 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 455 860 455
Size: 405 3755
NdeI CA'TA_TG
Cuts at: 324 324
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NspI r_CATG'y
Cuts at: 1213 1713 3316 1213
Size: 500 1603 2057
Fragments arranged by size:
2057 1603 500
PciI A'CATG_T
Cuts at: 3312 3312
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 318 318
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 4128
PshAI GACnn'nnGTC
Cuts at: 583 583
Size: 4160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 422 454 422
Size: 32 4128
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 1585 1945 1585
Size: 360 3800
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 439 2183 2393 439
Size: 1744 210 2206
Fragments arranged by size:
2206 1744 210
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 1097 1203 1888 2856 3047 33
Size: 1064 106 685 968 191 1146
Fragments arranged by size:
1146 1064 968 685 191 106
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1182 1182
Size: 4160
SmlI C'TyrA_G
Cuts at: 1315 2230 2944 3206 1315
Size: 915 714 262 2269
Fragments arranged by size:
2269 915 714 262
SpeI A'CTAG_T
Cuts at: 67 880 67
Size: 813 3347
SphI G_CATG'C
Cuts at: 1213 1713 1213
Size: 500 3660
StuI AGG'CCT
Cuts at: 838 838
Size: 4160
StyI C'CwwG_G
Cuts at: 119 455 860 1294 1648 119
Size: 336 405 434 354 2631
Fragments arranged by size:
2631 434 405 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1743 1876 2335 2544 1743
Size: 133 459 209 3359
Fragments arranged by size:
3359 459 209 133
TatI w'GTAC_w
Cuts at: 1075 3675 1075
Size: 2600 1560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 3677 3709 655
Size: 32 2990 32 1106
Fragments arranged by size:
2990 1106 32 32
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 462 1655 462
Size: 1193 2967
XmaI C'CCGG_G
Cuts at: 1180 1180
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PshAI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI