Restriction map for
pF1KB4391
(Circular) (Six-base) MAPSORT of: pF1KB4391.seq Check: 3232 from: 1 to: 4673
pF1KB4391 4673 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 919 2498 2930 3283 3945 613
Size: 306 1579 432 353 662 1341
Fragments arranged by size:
1579 1341 662 432 353 306
AfeI AGC'GCT
Cuts at: 1162 1162
Size: 4673
AflIII A'CryG_T
Cuts at: 607 2025 3825 4106 607
Size: 1418 1800 281 1174
Fragments arranged by size:
1800 1418 1174 281
AlwNI CAG_nnn'CTG
Cuts at: 137 1978 3416 137
Size: 1841 1438 1394
Fragments arranged by size:
1841 1438 1394
ApaLI G'TGCA_C
Cuts at: 1489 3511 1489
Size: 2022 2651
ApoI r'AATT_y
Cuts at: 594 895 981 1393 1677 4026 4282 594
Size: 301 86 412 284 2349 256 985
Fragments arranged by size:
2349 985 412 301 284 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1373 1406 1373
Size: 33 4640
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1689 2103 2351 2386 1689
Size: 414 248 35 3976
Fragments arranged by size:
3976 414 248 35
BanII G_rGCy'C
Cuts at: 130 1687 2717 130
Size: 1557 1030 2086
Fragments arranged by size:
2086 1557 1030
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1201 1201
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3627 4673 2571
Size: 1056 1046 2571
Fragments arranged by size:
2571 1056 1046
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 963 1785 963
Size: 822 3851
Bme1580I G_kGCm'C
Cuts at: 1493 2298 2391 3515 1493
Size: 805 93 1124 2651
Fragments arranged by size:
2651 1124 805 93
BmrI ACTGGGnnnn_n'
Cuts at: 1885 1966 2301 4445 1885
Size: 81 335 2144 2113
Fragments arranged by size:
2144 2113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1075 3040 1075
Size: 1965 2708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 114
Size: 4673
Bpu10I CC'TnA_GC
Cuts at: 124 124
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2728 3442 3740 1849
Size: 879 714 298 2782
Fragments arranged by size:
2782 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 1389 1632 2026 1389
Size: 243 394 4036
Fragments arranged by size:
4036 394 243
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 887 2104 2352 3054 4550 887
Size: 1217 248 702 1496 1010
Fragments arranged by size:
1496 1217 1010 702 248
BsaWI w'CCGG_w
Cuts at: 147 1862 2383 3061 3472 3619 3893 147
Size: 1715 521 678 411 147 274 927
Fragments arranged by size:
1715 927 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 112
Size: 30 4643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1606 1606
Size: 4673
BseYI C'CCAG_C
Cuts at: 429 2098 2856 3521 3929 429
Size: 1669 758 665 408 1173
Fragments arranged by size:
1669 1173 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3491 104
Size: 2111 46 1230 1286
Fragments arranged by size:
2111 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 130 1493 1687 2465 2655 3515 130
Size: 1363 194 778 190 860 1288
Fragments arranged by size:
1363 1288 860 778 194 190
BsmI GAATG_Cn'
Cuts at: 624 1055 624
Size: 431 4242
Bsp1286I G_dGCh'C
Cuts at: 130 1493 1687 2298 2391 2465 2655 2717 3515
Size: 1363 194 611 93 74 190 62 798
Cuts at: 3515 130
Size: 1288
Fragments arranged by size:
1363 1288 798 611 194 190 93 74
62
BspEI T'CCGG_A
Cuts at: 147 147
Size: 4673
BspHI T'CATG_A
Cuts at: 614 3105 614
Size: 2491 2182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1723 1937 2239 2620 1723
Size: 214 302 381 3776
Fragments arranged by size:
3776 381 302 214
BsrBI CCG'CTC
Cuts at: 50 962 2965 3019 4503 4668 50
Size: 912 2003 54 1484 165 55
Fragments arranged by size:
2003 1484 912 165 55 54
BsrDI GCAATG_nn'
Cuts at: 140 2585 140
Size: 2445 2228
BsrFI r'CCGG_y
Cuts at: 203 2671 2852 203
Size: 2468 181 2024
Fragments arranged by size:
2468 2024 181
BssSI C'ACGA_G
Cuts at: 2944 3652 2944
Size: 708 3965
BstAPI GCAn_nnn'nTGC
Cuts at: 200 2050 200
Size: 1850 2823
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstYI r'GATC_y
Cuts at: 1 1698 2165 2188 2523 2769 3075 3087 3173
Size: 1697 467 23 335 246 306 12 86
Cuts at: 3173 4225 1
Size: 1052 449
Fragments arranged by size:
1697 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 683 1494 107
Size: 576 811 3286
Fragments arranged by size:
3286 811 576
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 566 1916 566
Size: 1350 3323
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DraIII CAC_nnn'GTG
Cuts at: 787 787
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3723 2379
Size: 1344 3329
EaeI y'GGCC_r
Cuts at: 270 2258 2432 2823 2850 4195 270
Size: 1988 174 391 27 1345 748
Fragments arranged by size:
1988 1345 748 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 761 2696 2906 761
Size: 1935 210 2528
Fragments arranged by size:
2528 1935 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 732 2881 3623 3769 4483 732
Size: 2149 742 146 714 922
Fragments arranged by size:
2149 922 742 714 146
EcoICRI GAG'CTC
Cuts at: 128 1685 128
Size: 1557 3116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 613 919 2498 2930 3283 3945 114
Size: 499 306 1579 432 353 662 842
Fragments arranged by size:
1579 842 662 499 432 353 306
EcoO109I rG'GnC_Cy
Cuts at: 1812 1812
Size: 4673
EcoRI G'AATT_C
Cuts at: 895 981 1677 895
Size: 86 696 3891
Fragments arranged by size:
3891 696 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 1164 2107 2355 3585 4356 1164
Size: 943 248 1230 771 1481
Fragments arranged by size:
1481 1230 943 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2515 2545 2547 2577 2515
Size: 30 2 30 4611
Fragments arranged by size:
4611 30 30 2
HincII GTy'rAC
Cuts at: 1074 1712 1074
Size: 638 4035
HpaI GTT'AAC
Cuts at: 1074 1074
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 3432 3616 233
Size: 3199 184 1290
Fragments arranged by size:
3199 1290 184
MscI TGG'CCA
Cuts at: 272 2434 272
Size: 2162 2511
MslI CAynn'nnrTG
Cuts at: 1562 2662 2789 1562
Size: 1100 127 3446
Fragments arranged by size:
3446 1100 127
MspA1I CmG'CkG
Cuts at: 490 1480 1550 1784 2098 2458 3242 3487 490
Size: 990 70 234 314 360 784 245 1676
Fragments arranged by size:
1676 990 784 360 314 245 234 70
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 1494 107
Size: 1387 3286
NdeI CA'TA_TG
Cuts at: 1084 1084
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NspI r_CATG'y
Cuts at: 611 1726 2226 3829 611
Size: 1115 500 1603 1455
Fragments arranged by size:
1603 1455 1115 500
PciI A'CATG_T
Cuts at: 607 3825 607
Size: 3218 1455
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 4641
PstI C_TGCA'G
Cuts at: 196 977 1720 2405 196
Size: 781 743 685 2464
Fragments arranged by size:
2464 781 743 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 490 2098 2458 490
Size: 1608 360 2705
Fragments arranged by size:
2705 1608 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 130 1687 130
Size: 1557 3116
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 1572 4190 1572
Size: 2618 2055
SfcI C'TryA_G
Cuts at: 33 192 378 973 1716 2401 3369 3560 33
Size: 159 186 595 743 685 968 191 1146
Fragments arranged by size:
1146 968 743 685 595 191 186 159
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 1828 2743 3457 3719 1828
Size: 915 714 262 2782
Fragments arranged by size:
2782 915 714 262
SnaBI TAC'GTA
Cuts at: 1389 1389
Size: 4673
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StuI AGG'CCT
Cuts at: 587 1409 1615 587
Size: 822 206 3645
Fragments arranged by size:
3645 822 206
StyI C'CwwG_G
Cuts at: 107 220 738 1319 1494 1807 2161 107
Size: 113 518 581 175 313 354 2619
Fragments arranged by size:
2619 581 518 354 313 175 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2256 2389 2848 3057 2256
Size: 133 459 209 3872
Fragments arranged by size:
3872 459 209 133
TatI w'GTAC_w
Cuts at: 382 1570 4188 382
Size: 1188 2618 867
Fragments arranged by size:
2618 1188 867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 112 144 248 280 1483 1515 4190 4222 112
Size: 32 104 32 1203 32 2675 32 563
Fragments arranged by size:
2675 1203 563 104 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1099 1704 1099
Size: 605 4068
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 1648 2168 432
Size: 1216 520 2937
Fragments arranged by size:
2937 1216 520
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI