Restriction map for
pF1KB4394
(Circular) (Six-base) MAPSORT of: pF1KB4394.seq Check: 7932 from: 1 to: 5615
pF1KB4394 5615 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2270 2653 2270
Size: 383 5232
Acc65I G'GTAC_C
Cuts at: 1019 2631 1019
Size: 1612 4003
AclI AA'CG_TT
Cuts at: 5455 5455
Size: 5615
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1884 1910 2262 2443 3440 3872 4225 4887
Size: 1666 26 352 181 997 432 353 662
Cuts at: 4887 218
Size: 946
Fragments arranged by size:
1666 997 946 662 432 353 352 181
26
AflIII A'CryG_T
Cuts at: 1626 2967 4767 5048 1626
Size: 1341 1800 281 2193
Fragments arranged by size:
2193 1800 1341 281
AleI CACnn'nnGTG
Cuts at: 573 2402 573
Size: 1829 3786
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 878 2312 878
Size: 1434 4181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 1852 1884 244
Size: 32 1576 32 3975
Fragments arranged by size:
3975 1576 32 32
AlwNI CAG_nnn'CTG
Cuts at: 270 846 1206 1324 2313 2499 2920 4358 270
Size: 576 360 118 989 186 421 1438 1527
Fragments arranged by size:
1527 1438 989 576 421 360 186 118
ApaI G_GGCC'C
Cuts at: 2034 2251 2034
Size: 217 5398
ApaLI G'TGCA_C
Cuts at: 492 2393 4453 492
Size: 1901 2060 1654
Fragments arranged by size:
2060 1901 1654
ApoI r'AATT_y
Cuts at: 2059 2619 4968 5224 2059
Size: 560 2349 256 2450
Fragments arranged by size:
2450 2349 560 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5615
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
AvaI C'yCGr_G
Cuts at: 184 1679 2076 2119 2233 2244 2336 2635 184
Size: 1495 397 43 114 11 92 299 3164
Fragments arranged by size:
3164 1495 397 299 114 92 43 11
AvrII C'CTAG_G
Cuts at: 396 396
Size: 5615
BamHI G'GATC_C
Cuts at: 2640 2640
Size: 5615
BanI G'GyrC_C
Cuts at: 384 449 646 1019 1606 2503 2631 3045 3293
Size: 65 197 373 587 897 128 414 248
Cuts at: 3293 3328 384
Size: 35 2671
Fragments arranged by size:
2671 897 587 414 373 248 197 128
65 35
BanII G_rGCy'C
Cuts at: 170 243 782 984 1199 1477 1555 1966 2034
Size: 73 539 202 215 278 78 411 68
Cuts at: 2034 2251 2629 3659 170
Size: 217 378 1030 2126
Fragments arranged by size:
2126 1030 539 411 378 278 217 215
202 78 73 68
BbeI G_GCGC'C
Cuts at: 388 453 1610 3049 3297 388
Size: 65 1157 1439 248 2706
Fragments arranged by size:
2706 1439 1157 248 65
BbsI GAAGACnn'nnnn_
Cuts at: 1566 1658 1787 1566
Size: 92 129 5394
Fragments arranged by size:
5394 129 92
BbvCI CC'TCA_GC
Cuts at: 1264 1951 2342 1264
Size: 687 391 4537
Fragments arranged by size:
4537 687 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 297 331 1744 1778 297
Size: 34 1413 34 4134
Fragments arranged by size:
4134 1413 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1311 3513 4569 5615 1311
Size: 2202 1056 1046 1311
Fragments arranged by size:
2202 1311 1056 1046
BclI T'GATC_A
Cuts at: 1637 3135 1637
Size: 1498 4117
BglI GCCn_nnn'nGGC
Cuts at: 911 1615 2206 911
Size: 704 591 4320
Fragments arranged by size:
4320 704 591
BglII A'GATC_T
Cuts at: 3130 5167 3130
Size: 2037 3578
BlpI GC'TnA_GC
Cuts at: 2727 2727
Size: 5615
Bme1580I G_kGCm'C
Cuts at: 307 373 496 1417 2034 2120 2251 2397 2506
Size: 66 123 921 617 86 131 146 109
Cuts at: 2506 3240 3333 4457 307
Size: 734 93 1124 1465
Fragments arranged by size:
1465 1124 921 734 617 146 131 123
109 93 86 66
BmrI ACTGGGnnnn_n'
Cuts at: 1404 2827 2908 3243 5387 1404
Size: 1423 81 335 2144 1632
Fragments arranged by size:
2144 1632 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 210 2091 2613 3982 210
Size: 1881 522 1369 1843
Fragments arranged by size:
1881 1843 1369 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1021 1702 1948 2056 370
Size: 651 681 246 108 3929
Fragments arranged by size:
3929 681 651 246 108
Bpu10I CC'TnA_GC
Cuts at: 339 983 1264 1951 2342 339
Size: 644 281 687 391 3612
Fragments arranged by size:
3612 687 644 391 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 766 1792 2125 2791 3670 4384 4682 568
Size: 198 1026 333 666 879 714 298 1501
Fragments arranged by size:
1501 1026 879 714 666 333 298 198
BsaI GGTCTCn'nnnn_
Cuts at: 181 922 2244 5319 181
Size: 741 1322 3075 477
Fragments arranged by size:
3075 1322 741 477
BsaAI yAC'GTr
Cuts at: 975 1596 2968 975
Size: 621 1372 3622
Fragments arranged by size:
3622 1372 621
BsaBI GATnn'nnATC
Cuts at: 6 3129 5166 6
Size: 3123 2037 455
Fragments arranged by size:
3123 2037 455
BsaHI Gr'CG_yC
Cuts at: 355 385 450 830 1607 3046 3294 3996 5492
Size: 30 65 380 777 1439 248 702 1496
Cuts at: 5492 355
Size: 478
Fragments arranged by size:
1496 1439 777 702 478 380 248 65
30
BsaWI w'CCGG_w
Cuts at: 2804 3325 4003 4414 4561 4835 2804
Size: 521 678 411 147 274 3584
Fragments arranged by size:
3584 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 162 192 244 250 274 280 1447 1477 1660
Size: 30 52 6 24 6 1167 30 183
Cuts at: 1660 1690 162
Size: 30 4087
Fragments arranged by size:
4087 1167 183 52 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 271 535 835 949 1051 1207 1459 2221
Size: 3 264 300 114 102 156 252 762
Cuts at: 2221 268
Size: 3662
Fragments arranged by size:
3662 762 300 264 252 156 114 102
3
BseYI C'CCAG_C
Cuts at: 140 211 217 223 909 1053 1495 1732 2252
Size: 71 6 6 686 144 442 237 520
Cuts at: 2252 2261 2474 2600 3040 3798 4463 4871 140
Size: 9 213 126 440 758 665 408 884
Fragments arranged by size:
884 758 686 665 520 442 440 408
237 213 144 126 71 9 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 310 422 1126 1419 2338 101
Size: 209 112 704 293 919 3378
Fragments arranged by size:
3378 919 704 293 209 112
BsiEI CG_ry'CG
Cuts at: 104 515 899 3157 3203 4433 104
Size: 411 384 2258 46 1230 1286
Fragments arranged by size:
2258 1286 1230 411 384 46
BsiHKAI G_wGCw'C
Cuts at: 340 496 782 1477 1966 2397 2629 3407 3597
Size: 156 286 695 489 431 232 778 190
Cuts at: 3597 4457 340
Size: 860 1498
Fragments arranged by size:
1498 860 778 695 489 431 286 232
190 156
Bsp1286I G_dGCh'C
Cuts at: 170 243 307 340 373 496 782 984 1199
Size: 73 64 33 33 123 286 202 215
Cuts at: 1199 1417 1477 1555 1966 2034 2120 2251 2397
Size: 218 60 78 411 68 86 131 146
Cuts at: 2397 2506 2629 3240 3333 3407 3597 3659 4457
Size: 109 123 611 93 74 190 62 798
Cuts at: 4457 170
Size: 1328
Fragments arranged by size:
1328 798 611 411 286 218 215 202
190 146 131 123 123 109 93 86
78 74 73 68 64 62 60 33
33
BspHI T'CATG_A
Cuts at: 4047 4047
Size: 5615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2498 2665 2879 3181 3562 2498
Size: 167 214 302 381 4551
Fragments arranged by size:
4551 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 3907 3961 5445 5610 50
Size: 3857 54 1484 165 55
Fragments arranged by size:
3857 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 289 3527 289
Size: 3238 2377
BsrFI r'CCGG_y
Cuts at: 464 2408 3613 3794 464
Size: 1944 1205 181 2285
Fragments arranged by size:
2285 1944 1205 181
BssHII G'CGCG_C
Cuts at: 752 917 1097 752
Size: 165 180 5270
Fragments arranged by size:
5270 180 165
BssSI C'ACGA_G
Cuts at: 589 747 3886 4594 589
Size: 158 3139 708 1610
Fragments arranged by size:
3139 1610 708 158
BstAPI GCAn_nnn'nTGC
Cuts at: 368 500 716 846 2992 368
Size: 132 216 130 2146 2991
Fragments arranged by size:
2991 2146 216 132 130
BstBI TT'CG_AA
Cuts at: 3976 3976
Size: 5615
BstXI CCAn_nnnn'nTGG
Cuts at: 1053 1531 1053
Size: 478 5137
BstYI r'GATC_y
Cuts at: 1 803 2640 3107 3130 3465 3711 4017 4029
Size: 802 1837 467 23 335 246 306 12
Cuts at: 4029 4115 5167 1
Size: 86 1052 449
Fragments arranged by size:
1837 1052 802 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1741 1741
Size: 5615
BtgI C'CryG_G
Cuts at: 107 2192 2577 107
Size: 2085 385 3145
Fragments arranged by size:
3145 2085 385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3565 3746 3909 3565
Size: 181 163 5271
Fragments arranged by size:
5271 181 163
BtsI GCAGTG_nn'
Cuts at: 373 1707 2296 2858 373
Size: 1334 589 562 3130
Fragments arranged by size:
3130 1334 589 562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2550 2585 2550
Size: 35 5580
DraI TTT'AAA
Cuts at: 2614 2614
Size: 5615
DraIII CAC_nnn'GTG
Cuts at: 335 2370 335
Size: 2035 3580
DrdI GACnn_nn'nnGTC
Cuts at: 1363 3321 4665 1363
Size: 1958 1344 2313
Fragments arranged by size:
2313 1958 1344
EaeI y'GGCC_r
Cuts at: 512 896 903 1043 1598 2207 2574 3200 3374
Size: 384 7 140 555 609 367 626 174
Cuts at: 3374 3765 3792 5137 512
Size: 391 27 1345 990
Fragments arranged by size:
1345 990 626 609 555 391 384 367
174 140 27 7
EagI C'GGCC_G
Cuts at: 512 896 3200 512
Size: 384 2304 2927
Fragments arranged by size:
2927 2304 384
EarI CTCTTCn'nnn_
Cuts at: 1384 3638 3848 1384
Size: 2254 210 3151
Fragments arranged by size:
3151 2254 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 307 3823 4565 4711 5425 307
Size: 3516 742 146 714 497
Fragments arranged by size:
3516 742 714 497 146
EcoICRI GAG'CTC
Cuts at: 780 1475 1964 2627 780
Size: 695 489 663 3768
Fragments arranged by size:
3768 695 663 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 370 1021 1702 1884 1910 1948 2056 2262
Size: 152 651 681 182 26 38 108 206
Cuts at: 2262 2443 3440 3872 4225 4887 218
Size: 181 997 432 353 662 946
Fragments arranged by size:
997 946 681 662 651 432 353 206
182 181 152 108 38 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1165 1186 2233 2317 2524 1165
Size: 21 1047 84 207 4256
Fragments arranged by size:
4256 1047 207 84 21
EcoO109I rG'GnC_Cy
Cuts at: 137 147 1260 1341 1729 1746 2005 2031 2248
Size: 10 1113 81 388 17 259 26 217
Cuts at: 2248 2418 2594 2754 137
Size: 170 176 160 2998
Fragments arranged by size:
2998 1113 388 259 217 176 170 160
81 26 17 10
EcoRI G'AATT_C
Cuts at: 2059 2619 2059
Size: 560 5055
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 5615
FspI TGC'GCA
Cuts at: 482 616 1222 3396 482
Size: 134 606 2174 2701
Fragments arranged by size:
2701 2174 606 134
FspAI rTGC'GCAy
Cuts at: 1222 1222
Size: 5615
HaeII r_GCGC'y
Cuts at: 388 453 847 1174 1610 2192 2539 3049 3297
Size: 65 394 327 436 582 347 510 248
Cuts at: 3297 4527 5298 388
Size: 1230 771 705
Fragments arranged by size:
1230 771 705 582 510 436 394 347
327 248 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1032 1064 1252 1284 3457 3487 3489 3519 1032
Size: 32 188 32 2173 30 2 30 3128
Fragments arranged by size:
3128 2173 188 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1356 2654 1356
Size: 1298 4317
KasI G'GCGC_C
Cuts at: 384 449 1606 3045 3293 384
Size: 65 1157 1439 248 2706
Fragments arranged by size:
2706 1439 1157 248 65
KpnI G_GTAC'C
Cuts at: 1023 2635 1023
Size: 1612 4003
MluI A'CGCG_T
Cuts at: 1626 5048 1626
Size: 3422 2193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2504 4374 4558 701
Size: 1803 1870 184 1758
Fragments arranged by size:
1870 1803 1758 184
MscI TGG'CCA
Cuts at: 1045 1600 2209 3376 1045
Size: 555 609 1167 3284
Fragments arranged by size:
3284 1167 609 555
MslI CAynn'nnrTG
Cuts at: 573 1432 1529 2402 3604 3731 573
Size: 859 97 873 1202 127 2457
Fragments arranged by size:
2457 1202 873 859 127 97
MspA1I CmG'CkG
Cuts at: 447 500 738 909 1297 1722 1842 2256 2326
Size: 53 238 171 388 425 120 414 70
Cuts at: 2326 2726 3040 3400 4184 4429 447
Size: 400 314 360 784 245 1633
Fragments arranged by size:
1633 784 425 414 400 388 360 314
245 238 171 120 70 53
NaeI GCC'GGC
Cuts at: 3796 3796
Size: 5615
NarI GG'CG_CC
Cuts at: 385 450 1607 3046 3294 385
Size: 65 1157 1439 248 2706
Fragments arranged by size:
2706 1439 1157 248 65
NcoI C'CATG_G
Cuts at: 107 2192 107
Size: 2085 3530
NgoMIV G'CCGG_C
Cuts at: 3794 3794
Size: 5615
NspI r_CATG'y
Cuts at: 366 1152 2269 2668 3168 4771 366
Size: 786 1117 399 500 1603 1210
Fragments arranged by size:
1603 1210 1117 786 500 399
PasI CC'CwG_GG
Cuts at: 1281 1337 1281
Size: 56 5559
PciI A'CATG_T
Cuts at: 4767 4767
Size: 5615
PflMI CCAn_nnn'nTGG
Cuts at: 2572 2572
Size: 5615
PfoI T'CCnGG_A
Cuts at: 638 1748 2063 2105 638
Size: 1110 315 42 4148
Fragments arranged by size:
4148 1110 315 42
PmeI GTTT'AAAC
Cuts at: 2614 2614
Size: 5615
PmlI CAC'GTG
Cuts at: 975 1596 975
Size: 621 4994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 4061 4093 244
Size: 32 3785 32 1766
Fragments arranged by size:
3785 1766 32 32
PpuMI rG'GwC_Cy
Cuts at: 147 1260 1341 1746 147
Size: 1113 81 405 4016
Fragments arranged by size:
4016 1113 405 81
PspOMI G'GGCC_C
Cuts at: 2030 2247 2030
Size: 217 5398
PspXI vC'TCGA_Gb
Cuts at: 2233 2233
Size: 5615
PstI C_TGCA'G
Cuts at: 446 851 1079 1259 1943 2291 2309 2453 2495
Size: 405 228 180 684 348 18 144 42
Cuts at: 2495 2662 3347 446
Size: 167 685 2714
Fragments arranged by size:
2714 685 684 405 348 228 180 167
144 42 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5615
PvuII CAG'CTG
Cuts at: 909 1297 1722 1842 2256 2326 3040 3400 909
Size: 388 425 120 414 70 714 360 3124
Fragments arranged by size:
3124 714 425 414 388 360 120 70
RsrII CG'GwC_CG
Cuts at: 3810 3810
Size: 5615
SacI G_AGCT'C
Cuts at: 782 1477 1966 2629 782
Size: 695 489 663 3768
Fragments arranged by size:
3768 695 663 489
SalI G'TCGA_C
Cuts at: 2652 2652
Size: 5615
SanDI GG'GwC_CC
Cuts at: 1341 1341
Size: 5615
SapI GCTCTTCn'nnn_
Cuts at: 3638 3848 3638
Size: 210 5405
SbfI CC_TGCA'GG
Cuts at: 1259 2495 2662 1259
Size: 1236 167 4212
Fragments arranged by size:
4212 1236 167
ScaI AGT'ACT
Cuts at: 5132 5132
Size: 5615
SfcI C'TryA_G
Cuts at: 33 230 442 847 1075 1255 1939 2287 2305
Size: 197 212 405 228 180 684 348 18
Cuts at: 2305 2449 2491 2658 3343 4311 4502 33
Size: 144 42 167 685 968 191 1146
Fragments arranged by size:
1146 968 685 684 405 348 228 212
197 191 180 167 144 42 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 911 911
Size: 5615
SfoI GGC'GCC
Cuts at: 386 451 1608 3047 3295 386
Size: 65 1157 1439 248 2706
Fragments arranged by size:
2706 1439 1157 248 65
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
SmaI CCC'GGG
Cuts at: 2121 2246 2338 2637 2121
Size: 125 92 299 5099
Fragments arranged by size:
5099 299 125 92
SmlI C'TyrA_G
Cuts at: 583 781 1807 2140 2233 2770 3685 4399 4661
Size: 198 1026 333 93 537 915 714 262
Cuts at: 4661 583
Size: 1537
Fragments arranged by size:
1537 1026 915 714 537 333 262 198
93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5615
SphI G_CATG'C
Cuts at: 366 2668 3168 366
Size: 2302 500 2813
Fragments arranged by size:
2813 2302 500
StuI AGG'CCT
Cuts at: 2519 2519
Size: 5615
StyI C'CwwG_G
Cuts at: 107 396 1505 1913 2192 2749 3103 107
Size: 289 1109 408 279 557 354 2619
Fragments arranged by size:
2619 1109 557 408 354 289 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 946 1294 3198 3331 3790 3999 946
Size: 348 1904 133 459 209 2562
Fragments arranged by size:
2562 1904 459 348 209 133
TatI w'GTAC_w
Cuts at: 2429 5130 2429
Size: 2701 2914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 162 194 286 318 5132 5164 162
Size: 32 92 32 4814 32 613
Fragments arranged by size:
4814 613 92 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3412 3412
Size: 5615
XbaI T'CTAG_A
Cuts at: 2646 2646
Size: 5615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1600 3110 1600
Size: 1510 4105
XhoI C'TCGA_G
Cuts at: 2233 2233
Size: 5615
XmaI C'CCGG_G
Cuts at: 2119 2244 2336 2635 2119
Size: 125 92 299 5099
Fragments arranged by size:
5099 299 125 92
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AscI BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BstEII BstZ17I ClaI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI