Restriction map for
pF1KB4399
(Circular) (Six-base) MAPSORT of: pF1KB4399.seq Check: 5014 from: 1 to: 10097
pF1KB4399 10097 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4799 5654 6054 4799
Size: 855 400 8842
Fragments arranged by size:
8842 855 400
AccI GT'mk_AC
Cuts at: 7135 7135
Size: 10097
Acc65I G'GTAC_C
Cuts at: 1904 7113 1904
Size: 5209 4888
AclI AA'CG_TT
Cuts at: 9937 9937
Size: 10097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1122 1903 2311 2392 3668 4390 4996 5083
Size: 320 781 408 81 1276 722 606 87
Cuts at: 5083 5152 5817 5967 7922 8354 8707 9369 802
Size: 69 665 150 1955 432 353 662 1530
Fragments arranged by size:
1955 1530 1276 781 722 665 662 606
432 408 353 320 150 87 81 69
AfeI AGC'GCT
Cuts at: 6298 6298
Size: 10097
AflIII A'CryG_T
Cuts at: 5171 7449 9249 9530 5171
Size: 2278 1800 281 5738
Fragments arranged by size:
5738 2278 1800 281
AleI CACnn'nnGTG
Cuts at: 913 913
Size: 10097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3431 4286 4651 5015 5497 3431
Size: 855 365 364 482 8031
Fragments arranged by size:
8031 855 482 365 364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 493 525 493
Size: 32 10065
AlwNI CAG_nnn'CTG
Cuts at: 1128 1292 1569 1653 2583 2637 2931 3168 3243
Size: 164 277 84 930 54 294 237 75
Cuts at: 3243 3315 3394 3405 3534 3721 4674 4737 4845
Size: 72 79 11 129 187 953 63 108
Cuts at: 4845 5055 5484 5769 5990 6483 6713 7005 7402
Size: 210 429 285 221 493 230 292 397
Cuts at: 7402 8840 1128
Size: 1438 2385
Fragments arranged by size:
2385 1438 953 930 493 429 397 294
292 285 277 237 230 221 210 187
164 129 108 84 79 75 72 63
54 11
ApaI G_GGCC'C
Cuts at: 2448 5049 6689 2448
Size: 2601 1640 5856
Fragments arranged by size:
5856 2601 1640
ApaLI G'TGCA_C
Cuts at: 2974 5617 8935 2974
Size: 2643 3318 4136
Fragments arranged by size:
4136 3318 2643
ApoI r'AATT_y
Cuts at: 5821 7101 9450 9706 5821
Size: 1280 2349 256 6212
Fragments arranged by size:
6212 2349 1280 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10097
AvaI C'yCGr_G
Cuts at: 314 1678 4326 5315 5569 6150 6450 7117 314
Size: 1364 2648 989 254 581 300 667 3294
Fragments arranged by size:
3294 2648 1364 989 667 581 300 254
AvrII C'CTAG_G
Cuts at: 4506 4506
Size: 10097
BamHI G'GATC_C
Cuts at: 3725 7122 3725
Size: 3397 6700
BanI G'GyrC_C
Cuts at: 1667 1904 2237 3443 4431 4787 4960 5524 5789
Size: 237 333 1206 988 356 173 564 265
Cuts at: 5789 7113 7527 7775 7810 1667
Size: 1324 414 248 35 3954
Fragments arranged by size:
3954 1324 1206 988 564 414 356 333
265 248 237 173 35
BanII G_rGCy'C
Cuts at: 511 1349 1548 2165 2448 2594 3695 3965 4249
Size: 838 199 617 283 146 1101 270 284
Cuts at: 4249 4801 4922 5049 5462 5642 6197 6560 6605
Size: 552 121 127 413 180 555 363 45
Cuts at: 6605 6689 7111 8141 511
Size: 84 422 1030 2467
Fragments arranged by size:
2467 1101 1030 838 617 555 552 422
413 363 284 283 270 199 180 146
127 121 84 45
BbeI G_GCGC'C
Cuts at: 1671 4964 5793 7531 7779 1671
Size: 3293 829 1738 248 3989
Fragments arranged by size:
3989 3293 1738 829 248
BbsI GAAGACnn'nnnn_
Cuts at: 670 1805 2806 4225 670
Size: 1135 1001 1419 6542
Fragments arranged by size:
6542 1419 1135 1001
BbvCI CC'TCA_GC
Cuts at: 3658 3815 4895 6170 6880 3658
Size: 157 1080 1275 710 6875
Fragments arranged by size:
6875 1275 1080 710 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 305 329 339 957 991 1504 1538 5764
Size: 10 24 10 618 34 513 34 4226
Cuts at: 5764 5798 295
Size: 34 4594
Fragments arranged by size:
4594 4226 618 513 34 34 34 24
10 10
BciVI GTATCCnnnnn_n'
Cuts at: 3540 7995 9051 10097 3540
Size: 4455 1056 1046 3540
Fragments arranged by size:
4455 3540 1056 1046
BclI T'GATC_A
Cuts at: 7617 7617
Size: 10097
BglI GCCn_nnn'nGGC
Cuts at: 842 2443 3130 3643 3901 4861 5044 5596 6166
Size: 1601 687 513 258 960 183 552 570
Cuts at: 6166 6289 842
Size: 123 4650
Fragments arranged by size:
4650 1601 960 687 570 552 513 258
183 123
BglII A'GATC_T
Cuts at: 2081 2192 7612 9649 2081
Size: 111 5420 2037 2529
Fragments arranged by size:
5420 2529 2037 111
BlpI GC'TnA_GC
Cuts at: 213 627 830 1972 3395 4364 7209 213
Size: 414 203 1142 1423 969 2845 3101
Fragments arranged by size:
3101 2845 1423 1142 969 414 203
Bme1580I G_kGCm'C
Cuts at: 2240 2448 2978 3446 4790 5049 5621 6689 7722
Size: 208 530 468 1344 259 572 1068 1033
Cuts at: 7722 7815 8939 2240
Size: 93 1124 3398
Fragments arranged by size:
3398 1344 1124 1068 1033 572 530 468
259 208 93
BmgBI CAC'GTC
Cuts at: 3117 5139 5577 5679 6870 3117
Size: 2022 438 102 1191 6344
Fragments arranged by size:
6344 2022 1191 438 102
BmrI ACTGGGnnnn_n'
Cuts at: 4134 4605 6383 6542 7309 7390 7725 9869 4134
Size: 471 1778 159 767 81 335 2144 4362
Fragments arranged by size:
4362 2144 1778 767 471 335 159 81
BmtI G_CTAG'C
Cuts at: 1039 1039
Size: 10097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 408 4619 8464 408
Size: 4211 3845 2041
Fragments arranged by size:
4211 3845 2041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 416 451 466 517 690 829 1324 1570
Size: 13 35 15 51 173 139 495 246
Cuts at: 1570 1591 2026 2206 2308 2494 2503 2533 2767
Size: 21 435 180 102 186 9 30 234
Cuts at: 2767 2776 3127 3169 3556 3931 3949 5185 5233
Size: 9 351 42 387 375 18 1236 48
Cuts at: 5233 5476 6016 6154 6331 6398 6700 403
Size: 243 540 138 177 67 302 3800
Fragments arranged by size:
3800 1236 540 495 435 387 375 351
302 246 243 234 186 180 177 173
139 138 102 67 51 48 42 35
30 21 18 15 13 9 9
Bpu10I CC'TnA_GC
Cuts at: 1562 3185 3658 3815 4895 6170 6736 6880 1562
Size: 1623 473 157 1080 1275 566 144 4779
Fragments arranged by size:
4779 1623 1275 1080 566 473 157 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 3801 4488 4792 5176 6349 6365 7273 8152
Size: 3341 687 304 384 1173 16 908 879
Cuts at: 8152 8866 9164 460
Size: 714 298 1393
Fragments arranged by size:
3341 1393 1173 908 879 714 687 384
304 298 16
BsaI GGTCTCn'nnnn_
Cuts at: 1405 1504 1675 2995 5194 5314 5881 6351 9801
Size: 99 171 1320 2199 120 567 470 3450
Cuts at: 9801 1405
Size: 1701
Fragments arranged by size:
3450 2199 1701 1320 567 470 171 120
99
BsaAI yAC'GTr
Cuts at: 5280 7450 5280
Size: 2170 7927
BsaBI GATnn'nnATC
Cuts at: 6 433 7611 9648 6
Size: 427 7178 2037 455
Fragments arranged by size:
7178 2037 455 427
BsaHI Gr'CG_yC
Cuts at: 179 1115 1668 2390 2681 4853 4961 5588 5790
Size: 936 553 722 291 2172 108 627 202
Cuts at: 5790 7034 7528 7776 8478 9974 179
Size: 1244 494 248 702 1496 302
Fragments arranged by size:
2172 1496 1244 936 722 702 627 553
494 302 291 248 202 108
BsaWI w'CCGG_w
Cuts at: 1391 3728 7286 7807 8485 8896 9043 9317 1391
Size: 2337 3558 521 678 411 147 274 2171
Fragments arranged by size:
3558 2337 2171 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 493 523 688 718 922 952 3142 3172 3217
Size: 30 165 30 204 30 2190 30 45
Cuts at: 3217 3247 4915 4945 5158 5188 5839 5869 6922
Size: 30 1668 30 213 30 651 30 1053
Cuts at: 6922 6952 493
Size: 30 3638
Fragments arranged by size:
3638 2190 1668 1053 651 213 204 165
45 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 847 1546 1981 2491 2500 3013 3241 4420 4462
Size: 699 435 510 9 513 228 1179 42
Cuts at: 4462 4469 4606 4789 5842 5904 6055 6382 6400
Size: 7 137 183 1053 62 151 327 18
Cuts at: 6400 6940 6943 847
Size: 540 3 4001
Fragments arranged by size:
4001 1179 1053 699 540 513 510 435
327 228 183 151 137 62 42 18
9 7 3
BseYI C'CCAG_C
Cuts at: 173 840 1987 3161 3276 3721 4223 4410 5649
Size: 667 1147 1174 115 445 502 187 1239
Cuts at: 5649 5707 5798 6110 6287 7522 8280 8945 9353
Size: 58 91 312 177 1235 758 665 408
Cuts at: 9353 173
Size: 917
Fragments arranged by size:
1239 1235 1174 1147 917 758 667 665
502 445 408 312 187 177 115 91
58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2785 3874 4162 5488 5683 6664 1705
Size: 1080 1089 288 1326 195 981 5138
Fragments arranged by size:
5138 1326 1089 1080 981 288 195
BsiEI CG_ry'CG
Cuts at: 104 4829 5019 5600 7639 7685 8915 104
Size: 4725 190 581 2039 46 1230 1286
Fragments arranged by size:
4725 2039 1286 1230 581 190 46
BsiHKAI G_wGCw'C
Cuts at: 392 815 1349 2126 2165 2510 2594 2978 3965
Size: 423 534 777 39 345 84 384 987
Cuts at: 3965 4249 4801 4922 5462 5621 5642 6737 6961
Size: 284 552 121 540 159 21 1095 224
Cuts at: 6961 7111 7889 8079 8939 392
Size: 150 778 190 860 1550
Fragments arranged by size:
1550 1095 987 860 778 777 552 540
534 423 384 345 284 224 190 159
150 121 84 39 21
BsmI GAATG_Cn'
Cuts at: 817 3564 5364 817
Size: 2747 1800 5550
Fragments arranged by size:
5550 2747 1800
BsmBI CGTCTCn'nnnn_
Cuts at: 4570 4570
Size: 10097
Bsp1286I G_dGCh'C
Cuts at: 392 511 815 1349 1548 2126 2165 2240 2448
Size: 119 304 534 199 578 39 75 208
Cuts at: 2448 2510 2594 2978 3446 3695 3965 4249 4790
Size: 62 84 384 468 249 270 284 541
Cuts at: 4790 4801 4922 5049 5462 5621 5642 6197 6560
Size: 11 121 127 413 159 21 555 363
Cuts at: 6560 6605 6689 6737 6961 7111 7722 7815 7889
Size: 45 84 48 224 150 611 93 74
Cuts at: 7889 8079 8141 8939 392
Size: 190 62 798 1550
Fragments arranged by size:
1550 798 611 578 555 541 534 468
413 384 363 304 284 270 249 224
208 199 190 159 150 127 121 119
93 84 84 75 74 62 62 48
45 39 21 11
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 10097
BspHI T'CATG_A
Cuts at: 4655 5930 8529 4655
Size: 1275 2599 6223
Fragments arranged by size:
6223 2599 1275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 1055 1652 1811 2630 3061 3409 4262 4799
Size: 696 597 159 819 431 348 853 537
Cuts at: 4799 5510 5654 6054 6491 7147 7361 7663 8044
Size: 711 144 400 437 656 214 302 381
Cuts at: 8044 359
Size: 2412
Fragments arranged by size:
2412 853 819 711 696 656 597 537
437 431 400 381 348 302 214 159
144
BsrBI CCG'CTC
Cuts at: 50 261 855 1536 2943 5343 5634 6117 8389
Size: 211 594 681 1407 2400 291 483 2272
Cuts at: 8389 8443 9927 10092 50
Size: 54 1484 165 55
Fragments arranged by size:
2400 2272 1484 1407 681 594 483 291
211 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1409 8009 1409
Size: 6600 3497
BsrFI r'CCGG_y
Cuts at: 752 2396 4181 6615 8095 8276 752
Size: 1644 1785 2434 1480 181 2573
Fragments arranged by size:
2573 2434 1785 1644 1480 181
BsrGI T'GTAC_A
Cuts at: 489 1247 2657 489
Size: 758 1410 7929
Fragments arranged by size:
7929 1410 758
BssHII G'CGCG_C
Cuts at: 2221 2221
Size: 10097
BssSI C'ACGA_G
Cuts at: 1438 8368 9076 1438
Size: 6930 708 2459
Fragments arranged by size:
6930 2459 708
BstAPI GCAn_nnn'nTGC
Cuts at: 440 1128 1653 2583 2637 3243 3255 3405 4644
Size: 688 525 930 54 606 12 150 1239
Cuts at: 4644 5769 6642 7474 440
Size: 1125 873 832 3063
Fragments arranged by size:
3063 1239 1125 930 873 832 688 606
525 150 54 12
BstBI TT'CG_AA
Cuts at: 8458 8458
Size: 10097
BstXI CCAn_nnnn'nTGG
Cuts at: 2847 6777 2847
Size: 3930 6167
BstYI r'GATC_y
Cuts at: 1 2081 2192 3312 3725 4940 5531 6044 7122
Size: 2080 111 1120 413 1215 591 513 1078
Cuts at: 7122 7589 7612 7947 8193 8499 8511 8597 9649
Size: 467 23 335 246 306 12 86 1052
Cuts at: 9649 1
Size: 449
Fragments arranged by size:
2080 1215 1120 1078 1052 591 513 467
449 413 335 306 246 111 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 5075 5075
Size: 10097
BtgI C'CryG_G
Cuts at: 1154 2166 2493 4298 4702 6278 6551 6622 6630
Size: 1012 327 1805 404 1576 273 71 8
Cuts at: 6630 6798 6912 1154
Size: 168 114 4339
Fragments arranged by size:
4339 1805 1576 1012 404 327 273 168
114 71 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1821 2372 3063 5394 8047 8228 8391 1821
Size: 551 691 2331 2653 181 163 3527
Fragments arranged by size:
3527 2653 2331 691 551 181 163
BtsI GCAGTG_nn'
Cuts at: 438 965 1693 1963 4672 5224 7340 438
Size: 527 728 270 2709 552 2116 3195
Fragments arranged by size:
3195 2709 2116 728 552 527 270
ClaI AT'CG_AT
Cuts at: 4103 4103
Size: 10097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 108 143 3017 3052 6309 6344 108
Size: 35 2874 35 3257 35 3861
Fragments arranged by size:
3861 3257 2874 35 35 35
DraI TTT'AAA
Cuts at: 7096 7096
Size: 10097
DraIII CAC_nnn'GTG
Cuts at: 2912 5616 6228 2912
Size: 2704 612 6781
Fragments arranged by size:
6781 2704 612
DrdI GACnn_nn'nnGTC
Cuts at: 595 1924 2758 2934 7803 9147 595
Size: 1329 834 176 4869 1344 1545
Fragments arranged by size:
4869 1545 1344 1329 834 176
EaeI y'GGCC_r
Cuts at: 116 2720 2859 3119 4826 5016 5036 5402 5486
Size: 2604 139 260 1707 190 20 366 84
Cuts at: 5486 5597 5681 6203 6281 6431 7682 7856 8247
Size: 111 84 522 78 150 1251 174 391
Cuts at: 8247 8274 9619 116
Size: 27 1345 594
Fragments arranged by size:
2604 1707 1345 1251 594 522 391 366
260 190 174 150 139 111 84 84
78 27 20
EagI C'GGCC_G
Cuts at: 4826 5016 5597 7682 4826
Size: 190 581 2085 7241
Fragments arranged by size:
7241 2085 581 190
EarI CTCTTCn'nnn_
Cuts at: 1711 2534 4949 5141 5253 5291 6455 6913 7057
Size: 823 2415 192 112 38 1164 458 144
Cuts at: 7057 8120 8330 1711
Size: 1063 210 3478
Fragments arranged by size:
3478 2415 1164 1063 823 458 210 192
144 112 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2007 2632 4765 5158 5830 8305 9047 9193 9907
Size: 625 2133 393 672 2475 742 146 714
Cuts at: 9907 2007
Size: 2197
Fragments arranged by size:
2475 2197 2133 742 714 672 625 393
146
EcoICRI GAG'CTC
Cuts at: 1347 2163 2592 3963 4247 4799 4920 5460 5640
Size: 816 429 1371 284 552 121 540 180
Cuts at: 5640 7109 1347
Size: 1469 4335
Fragments arranged by size:
4335 1469 1371 816 552 540 429 284
180 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 416 451 466 517 690 802 829 1122
Size: 13 35 15 51 173 112 27 293
Cuts at: 1122 1324 1570 1591 1903 2026 2206 2308 2311
Size: 202 246 21 312 123 180 102 3
Cuts at: 2311 2392 2494 2503 2533 2767 2776 3127 3169
Size: 81 102 9 30 234 9 351 42
Cuts at: 3169 3556 3668 3931 3949 4390 4996 5083 5152
Size: 387 112 263 18 441 606 87 69
Cuts at: 5152 5185 5233 5476 5817 5967 6016 6154 6331
Size: 33 48 243 341 150 49 138 177
Cuts at: 6331 6398 6700 7922 8354 8707 9369 403
Size: 67 302 1222 432 353 662 1131
Fragments arranged by size:
1222 1131 662 606 441 432 387 353
351 341 312 302 293 263 246 243
234 202 180 177 173 150 138 123
112 112 102 102 87 81 69 67
51 49 48 42 35 33 30 27
21 18 15 13 9 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1561 2788 3388 4806 6565 1561
Size: 1227 600 1418 1759 5093
Fragments arranged by size:
5093 1759 1418 1227 600
EcoO109I rG'GnC_Cy
Cuts at: 233 348 1289 1554 2295 2445 2580 2751 3063
Size: 115 941 265 741 150 135 171 312
Cuts at: 3063 3240 3540 4335 4767 5052 6570 6685 6710
Size: 177 300 795 432 285 1518 115 25
Cuts at: 6710 7236 233
Size: 526 3094
Fragments arranged by size:
3094 1518 941 795 741 526 432 312
300 285 265 177 171 150 135 115
115 25
EcoRI G'AATT_C
Cuts at: 7101 7101
Size: 10097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 738 1219 6888 8116 738
Size: 481 5669 1228 2719
Fragments arranged by size:
5669 2719 1228 481
FspI TGC'GCA
Cuts at: 433 7878 433
Size: 7445 2652
FspAI rTGC'GCAy
Cuts at: 433 433
Size: 10097
HaeII r_GCGC'y
Cuts at: 837 1386 1437 1671 4298 4782 4964 5770 5793
Size: 549 51 234 2627 484 182 806 23
Cuts at: 5793 6300 7531 7779 9009 9780 837
Size: 507 1231 248 1230 771 1154
Fragments arranged by size:
2627 1231 1230 1154 806 771 549 507
484 248 234 182 51 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 883 915 1737 1769 3142 3174 5839
Size: 32 655 32 822 32 1373 32 2665
Cuts at: 5839 5871 5899 5931 6363 6395 6430 6462 6815
Size: 32 28 32 432 32 35 32 353
Cuts at: 6815 6847 6922 6954 7939 7969 7971 8001 196
Size: 32 75 32 985 30 2 30 2292
Fragments arranged by size:
2665 2292 1373 985 822 655 432 353
75 35 32 32 32 32 32 32
32 32 32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 7136 7136
Size: 10097
KasI G'GCGC_C
Cuts at: 1667 4960 5789 7527 7775 1667
Size: 3293 829 1738 248 3989
Fragments arranged by size:
3989 3293 1738 829 248
KpnI G_GTAC'C
Cuts at: 1908 7117 1908
Size: 5209 4888
MluI A'CGCG_T
Cuts at: 9530 9530
Size: 10097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2918 4274 6728 8856 9040 1769
Size: 1149 1356 2454 2128 184 2826
Fragments arranged by size:
2826 2454 2128 1356 1149 184
MscI TGG'CCA
Cuts at: 2861 5038 5683 6205 7858 2861
Size: 2177 645 522 1653 5100
Fragments arranged by size:
5100 2177 1653 645 522
MslI CAynn'nnrTG
Cuts at: 686 913 4969 5473 6515 8086 8213 686
Size: 227 4056 504 1042 1571 127 2570
Fragments arranged by size:
4056 2570 1571 1042 504 227 127
MspA1I CmG'CkG
Cuts at: 173 710 840 1031 1128 1474 1987 2289 3168
Size: 537 130 191 97 346 513 302 879
Cuts at: 3168 3210 3294 3531 3858 4122 4311 4527 4704
Size: 42 84 237 327 264 189 216 177
Cuts at: 4704 4737 4825 5034 5481 6021 6110 6209 6287
Size: 33 88 209 447 540 89 99 78
Cuts at: 6287 6624 7208 7522 7882 8666 8911 173
Size: 337 584 314 360 784 245 1359
Fragments arranged by size:
1359 879 784 584 540 537 513 447
360 346 337 327 314 302 264 245
237 216 209 191 189 177 130 99
97 89 88 84 78 42 33
NaeI GCC'GGC
Cuts at: 6617 8278 6617
Size: 1661 8436
NarI GG'CG_CC
Cuts at: 1668 4961 5790 7528 7776 1668
Size: 3293 829 1738 248 3989
Fragments arranged by size:
3989 3293 1738 829 248
NcoI C'CATG_G
Cuts at: 4298 6630 6798 6912 4298
Size: 2332 168 114 7483
Fragments arranged by size:
7483 2332 168 114
NdeI CA'TA_TG
Cuts at: 5703 5703
Size: 10097
NgoMIV G'CCGG_C
Cuts at: 6615 8276 6615
Size: 1661 8436
NheI G'CTAG_C
Cuts at: 1035 1035
Size: 10097
NspI r_CATG'y
Cuts at: 369 1719 3375 3418 5175 5478 6129 6829 7150
Size: 1350 1656 43 1757 303 651 700 321
Cuts at: 7150 7650 9253 369
Size: 500 1603 1213
Fragments arranged by size:
1757 1656 1603 1350 1213 700 651 500
321 303 43
PasI CC'CwG_GG
Cuts at: 2448 2526 3564 5049 5903 6783 2448
Size: 78 1038 1485 854 880 5762
Fragments arranged by size:
5762 1485 1038 880 854 78
PciI A'CATG_T
Cuts at: 5171 9249 5171
Size: 4078 6019
PflMI CCAn_nnn'nTGG
Cuts at: 444 540 2604 3011 3534 4167 5649 5679 5805
Size: 96 2064 407 523 633 1482 30 126
Cuts at: 5805 444
Size: 4736
Fragments arranged by size:
4736 2064 1482 633 523 407 126 96
30
PfoI T'CCnGG_A
Cuts at: 235 1219 1709 1931 2198 2324 2471 2783 3617
Size: 984 490 222 267 126 147 312 834
Cuts at: 3617 4487 4496 5303 5453 5672 5857 6073 6712
Size: 870 9 807 150 219 185 216 639
Cuts at: 6712 235
Size: 3620
Fragments arranged by size:
3620 984 870 834 807 639 490 312
267 222 219 216 185 150 147 126
9
PmeI GTTT'AAAC
Cuts at: 7096 7096
Size: 10097
PmlI CAC'GTG
Cuts at: 5280 5280
Size: 10097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 493 525 4468 4500 6459 6491 8543 8575 493
Size: 32 3943 32 1959 32 2052 32 2015
Fragments arranged by size:
3943 2052 2015 1959 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 233 348 1554 2295 2751 3063 4335 4767 6570
Size: 115 1206 741 456 312 1272 432 1803
Cuts at: 6570 6710 233
Size: 140 3620
Fragments arranged by size:
3620 1803 1272 1206 741 456 432 312
140 115
PshAI GACnn'nnGTC
Cuts at: 1114 2624 4345 1114
Size: 1510 1721 6866
Fragments arranged by size:
6866 1721 1510
PspOMI G'GGCC_C
Cuts at: 2444 5045 6685 2444
Size: 2601 1640 5856
Fragments arranged by size:
5856 2601 1640
PspXI vC'TCGA_Gb
Cuts at: 314 314
Size: 10097
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2650 2682 5167 5199 6709 6741 2650
Size: 32 2485 32 1510 32 6006
Fragments arranged by size:
6006 2485 1510 32 32 32
PstI C_TGCA'G
Cuts at: 1133 1649 1661 1700 2579 2642 3248 3260 3299
Size: 516 12 39 879 63 606 12 39
Cuts at: 3299 3311 3410 3717 4202 4553 4679 4724 4796
Size: 12 99 307 485 351 126 45 72
Cuts at: 4796 4841 5067 5696 6020 6062 6488 6950 7144
Size: 45 226 629 324 42 426 462 194
Cuts at: 7144 7829 1133
Size: 685 3401
Fragments arranged by size:
3401 879 685 629 606 516 485 462
426 351 324 307 226 194 126 99
72 63 45 45 42 39 39 12
12 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10097
PvuII CAG'CTG
Cuts at: 710 840 1128 1474 1987 2289 3168 3294 3858
Size: 130 288 346 513 302 879 126 564
Cuts at: 3858 4122 4311 4527 4737 5034 5481 6287 7522
Size: 264 189 216 210 297 447 806 1235
Cuts at: 7522 7882 710
Size: 360 2925
Fragments arranged by size:
2925 1235 879 806 564 513 447 360
346 302 297 288 264 216 210 189
130 126
RsrII CG'GwC_CG
Cuts at: 8292 8292
Size: 10097
SacI G_AGCT'C
Cuts at: 1349 2165 2594 3965 4249 4801 4922 5462 5642
Size: 816 429 1371 284 552 121 540 180
Cuts at: 5642 7111 1349
Size: 1469 4335
Fragments arranged by size:
4335 1469 1371 816 552 540 429 284
180 121
SacII CC_GC'GG
Cuts at: 4705 6625 4705
Size: 1920 8177
SalI G'TCGA_C
Cuts at: 7134 7134
Size: 10097
SapI GCTCTTCn'nnn_
Cuts at: 1711 5141 6913 8120 8330 1711
Size: 3430 1772 1207 210 3478
Fragments arranged by size:
3478 3430 1772 1207 210
SbfI CC_TGCA'GG
Cuts at: 1649 3260 3311 7144 1649
Size: 1611 51 3833 4602
Fragments arranged by size:
4602 3833 1611 51
ScaI AGT'ACT
Cuts at: 4873 9614 4873
Size: 4741 5356
SexAI A'CCwGG_T
Cuts at: 2908 2908
Size: 10097
SfcI C'TryA_G
Cuts at: 33 1129 1645 1657 1696 1807 2575 2638 3244
Size: 1096 516 12 39 111 768 63 606
Cuts at: 3244 3256 3295 3307 3406 3713 3934 4198 4549
Size: 12 39 12 99 307 221 264 351
Cuts at: 4549 4675 4720 4792 4837 5063 5692 6016 6058
Size: 126 45 72 45 226 629 324 42
Cuts at: 6058 6484 6946 7140 7825 8793 8984 33
Size: 426 462 194 685 968 191 1146
Fragments arranged by size:
1146 1096 968 768 685 629 606 516
462 426 351 324 307 264 226 221
194 191 126 111 99 72 63 45
45 42 39 39 12 12 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2443 3901 5044 2443
Size: 1458 1143 7496
Fragments arranged by size:
7496 1458 1143
SfoI GGC'GCC
Cuts at: 1669 4962 5791 7529 7777 1669
Size: 3293 829 1738 248 3989
Fragments arranged by size:
3989 3293 1738 829 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10097
SmaI CCC'GGG
Cuts at: 4328 5317 6452 7119 4328
Size: 989 1135 667 7306
Fragments arranged by size:
7306 1135 989 667
SmlI C'TyrA_G
Cuts at: 314 475 1678 3780 4467 4771 5191 6150 6328
Size: 161 1203 2102 687 304 420 959 178
Cuts at: 6328 6380 7252 8167 8881 9143 314
Size: 52 872 915 714 262 1268
Fragments arranged by size:
2102 1268 1203 959 915 872 714 687
420 304 262 178 161 52
SpeI A'CTAG_T
Cuts at: 67 1269 67
Size: 1202 8895
SphI G_CATG'C
Cuts at: 369 3418 5478 7150 7650 369
Size: 3049 2060 1672 500 2816
Fragments arranged by size:
3049 2816 2060 1672 500
StuI AGG'CCT
Cuts at: 247 1135 2149 3301 3331 3904 4900 6034 247
Size: 888 1014 1152 30 573 996 1134 4310
Fragments arranged by size:
4310 1152 1134 1014 996 888 573 30
StyI C'CwwG_G
Cuts at: 248 419 632 1845 3492 3609 4298 4506 4946
Size: 171 213 1213 1647 117 689 208 440
Cuts at: 4946 6630 6798 6912 7231 7585 248
Size: 1684 168 114 319 354 2760
Fragments arranged by size:
2760 1684 1647 1213 689 440 354 319
213 208 171 168 117 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 285 1528 2879 3361 4575 5218 5641 7680 7813
Size: 1243 1351 482 1214 643 423 2039 133
Cuts at: 7813 8272 8481 285
Size: 459 209 1901
Fragments arranged by size:
2039 1901 1351 1243 1214 643 482 459
423 209 133
TatI w'GTAC_w
Cuts at: 489 1025 1247 2657 4871 6725 9612 489
Size: 536 222 1410 2214 1854 2887 974
Fragments arranged by size:
2887 2214 1854 1410 974 536 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 922 954 3217 3249 6212 6244 9614 9646 922
Size: 32 2263 32 2963 32 3370 32 1373
Fragments arranged by size:
3370 2963 2263 1373 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7894 7894
Size: 10097
XbaI T'CTAG_A
Cuts at: 7128 7128
Size: 10097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1453 2206 2445 3051 7592 1453
Size: 753 239 606 4541 3958
Fragments arranged by size:
4541 3958 753 606 239
XhoI C'TCGA_G
Cuts at: 314 1678 6150 314
Size: 1364 4472 4261
Fragments arranged by size:
4472 4261 1364
XmaI C'CCGG_G
Cuts at: 4326 5315 6450 7117 4326
Size: 989 1135 667 7306
Fragments arranged by size:
7306 1135 989 667
XmnI GAAnn'nnTTC
Cuts at: 269 1705 2032 2341 2543 4483 5362 7066 269
Size: 1436 327 309 202 1940 879 1704 3300
Fragments arranged by size:
3300 1940 1704 1436 879 327 309 202
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AscI BaeI BfrBI BsiWI
BstEII BstZ17I EcoRV FseI HindIII HpaI MfeI NotI
NruI NsiI PacI PsiI SanDI SgrAI SnaBI SrfI
SspI SwaI ZraI