Restriction map for
pF1KB4401
(Circular) (Six-base) MAPSORT of: pF1KB4401.seq Check: 8250 from: 1 to: 3722
pF1KB4401 3722 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 716 3562 716
Size: 2846 876
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1547 1979 2726 2994 539
Size: 1008 432 747 268 1267
Fragments arranged by size:
1267 1008 747 432 268
AflIII A'CryG_T
Cuts at: 542 1074 2178 3155 542
Size: 532 1104 977 1109
Fragments arranged by size:
1109 1104 977 532
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 3722
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 640 672 640
Size: 32 3690
AlwNI CAG_nnn'CTG
Cuts at: 1027 2594 1027
Size: 1567 2155
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 523 726 3075 3331 523
Size: 203 2349 256 914
Fragments arranged by size:
2349 914 256 203
AseI AT'TA_AT
Cuts at: 20 266 20
Size: 246 3476
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 742 742
Size: 3722
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 738 1152 1400 1435 738
Size: 414 248 35 3025
Fragments arranged by size:
3025 414 248 35
BanII G_rGCy'C
Cuts at: 736 1766 736
Size: 1030 2692
BbeI G_GCGC'C
Cuts at: 1156 1404 1156
Size: 248 3474
BbsI GAAGACnn'nnnn_
Cuts at: 178 178
Size: 3722
BbvCI CC'TCA_GC
Cuts at: 561 561
Size: 3722
BciVI GTATCCnnnnn_n'
Cuts at: 666 1620 2381 3722 666
Size: 954 761 1341 666
Fragments arranged by size:
1341 954 761 666
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglII A'GATC_T
Cuts at: 290 1237 3274 290
Size: 947 2037 738
Fragments arranged by size:
2037 947 738
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 1347 1440 2496 1347
Size: 93 1056 2573
Fragments arranged by size:
2573 1056 93
BmgBI CAC'GTC
Cuts at: 176 176
Size: 3722
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 3722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 692 574
Size: 118 3604
Bpu10I CC'TnA_GC
Cuts at: 536 561 536
Size: 25 3697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 1777 2269 2567 898
Size: 879 492 298 2053
Fragments arranged by size:
2053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 550 3426 550
Size: 2876 846
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 1153 1401 2103 3599 1153
Size: 248 702 1496 1276
Fragments arranged by size:
1496 1276 702 248
BsaWI w'CCGG_w
Cuts at: 911 1432 2110 2384 2531 2942 911
Size: 521 678 274 147 411 1691
Fragments arranged by size:
1691 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 506 1147 1905 2482 2978 506
Size: 641 758 577 496 1250
Fragments arranged by size:
1250 758 641 577 496
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 736 1514 1704 2496 736
Size: 778 190 792 1962
Fragments arranged by size:
1962 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 736 1347 1440 1514 1704 1766 2496 736
Size: 611 93 74 190 62 730 1962
Fragments arranged by size:
1962 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 295 772 986 1288 1669 295
Size: 477 214 302 381 2348
Fragments arranged by size:
2348 477 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2014 2068 3552 3717 50
Size: 1964 54 1484 165 55
Fragments arranged by size:
1964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 1720 1901 2110 1720
Size: 181 209 3332
Fragments arranged by size:
3332 209 181
BssSI C'ACGA_G
Cuts at: 1993 2351 1993
Size: 358 3364
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3722
BstYI r'GATC_y
Cuts at: 1 290 747 1214 1237 1572 1818 2830 2916
Size: 289 457 467 23 335 246 1012 86
Cuts at: 2916 2928 3274 1
Size: 12 346 449
Fragments arranged by size:
1012 467 457 449 346 335 289 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1672 1853 2016 1672
Size: 181 163 3378
Fragments arranged by size:
3378 181 163
BtsI GCAGTG_nn'
Cuts at: 965 965
Size: 3722
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 1307 1481 1872 1899 3244 1307
Size: 174 391 27 1345 1785
Fragments arranged by size:
1785 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 598 1745 1955 598
Size: 1147 210 2365
Fragments arranged by size:
2365 1147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1930 2240 2386 3532 1930
Size: 310 146 1146 2120
Fragments arranged by size:
2120 1146 310 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 574 692 1547 1979 2726 2994 539
Size: 35 118 855 432 747 268 1267
Fragments arranged by size:
1267 855 747 432 268 118 35
EcoNI CCTnn'n_nnAGG
Cuts at: 674 674
Size: 3722
EcoO109I rG'GnC_Cy
Cuts at: 861 861
Size: 3722
EcoRI G'AATT_C
Cuts at: 523 726 523
Size: 203 3519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 353 1156 1404 2426 3405 353
Size: 803 248 1022 979 670
Fragments arranged by size:
1022 979 803 670 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1564 1594 1596 1626 1564
Size: 30 2 30 3660
Fragments arranged by size:
3660 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 1152 1400 1152
Size: 248 3474
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MfeI C'AATT_G
Cuts at: 230 230
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2577 2393
Size: 184 3538
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 112 457 643 1711 1838 112
Size: 345 186 1068 127 1996
Fragments arranged by size:
1996 1068 345 186 127
MspA1I CmG'CkG
Cuts at: 833 1147 1507 2520 2765 833
Size: 314 360 1013 245 1790
Fragments arranged by size:
1790 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 1153 1401 1153
Size: 248 3474
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 546 611 775 1275 2182 546
Size: 65 164 500 907 2086
Fragments arranged by size:
2086 907 500 164 65
PciI A'CATG_T
Cuts at: 542 2178 542
Size: 1636 2086
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PstI C_TGCA'G
Cuts at: 769 1454 769
Size: 685 3037
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 1917 1917
Size: 3722
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SfcI C'TryA_G
Cuts at: 33 286 765 1450 2443 2634 33
Size: 253 479 685 993 191 1121
Fragments arranged by size:
1121 993 685 479 253 191
SfoI GGC'GCC
Cuts at: 1154 1402 1154
Size: 248 3474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 877 1792 2284 2546 877
Size: 915 492 262 2053
Fragments arranged by size:
2053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 611 775 1275 611
Size: 164 500 3058
Fragments arranged by size:
3058 500 164
StyI C'CwwG_G
Cuts at: 566 856 1210 566
Size: 290 354 3078
Fragments arranged by size:
3078 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 311 1305 1438 1897 2106 311
Size: 994 133 459 209 1927
Fragments arranged by size:
1927 994 459 209 133
TatI w'GTAC_w
Cuts at: 3237 3237
Size: 3722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 3690
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
XmnI GAAnn'nnTTC
Cuts at: 592 592
Size: 3722
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI