Restriction map for
pF1KB4411
(Circular) (Six-base) MAPSORT of: pF1KB4411.seq Check: 8131 from: 1 to: 3671
pF1KB4411 3671 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 3511 3511
Size: 3671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 1928 2675 2943 1496
Size: 432 747 268 2224
Fragments arranged by size:
2224 747 432 268
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 144 144
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 262 546 976 2543 262
Size: 284 430 1567 1390
Fragments arranged by size:
1567 1390 430 284
ApaI G_GGCC'C
Cuts at: 660 660
Size: 3671
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 675 3024 3280 675
Size: 2349 256 1066
Fragments arranged by size:
2349 1066 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3671
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 521 691 521
Size: 170 3501
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 687 1101 1349 1384 687
Size: 414 248 35 2974
Fragments arranged by size:
2974 414 248 35
BanII G_rGCy'C
Cuts at: 198 660 685 1715 198
Size: 462 25 1030 2154
Fragments arranged by size:
2154 1030 462 25
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BbsI GAAGACnn'nnnn_
Cuts at: 253 253
Size: 3671
BbvCI CC'TCA_GC
Cuts at: 515 515
Size: 3671
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BfrBI ATG'CAT
Cuts at: 131 131
Size: 3671
BglII A'GATC_T
Cuts at: 176 1186 3223 176
Size: 1010 2037 624
Fragments arranged by size:
2037 1010 624
BlpI GC'TnA_GC
Cuts at: 783 783
Size: 3671
Bme1580I G_kGCm'C
Cuts at: 660 1296 1389 2445 660
Size: 636 93 1056 1886
Fragments arranged by size:
1886 1056 636 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 439 425
Size: 14 3657
Bpu10I CC'TnA_GC
Cuts at: 515 515
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 137 3375 137
Size: 3238 433
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 263 293 263
Size: 30 3641
BseYI C'CCAG_C
Cuts at: 147 534 1096 1854 2431 2927 147
Size: 387 562 758 577 496 891
Fragments arranged by size:
891 758 577 562 496 387
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 198 660 685 1296 1389 1463 1653 1715 2445
Size: 462 25 611 93 74 190 62 730
Cuts at: 2445 198
Size: 1424
Fragments arranged by size:
1424 730 611 462 190 93 74 62
25
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 935 1237 1618 721
Size: 214 302 381 2774
Fragments arranged by size:
2774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 490 1669 1850 2059 490
Size: 1179 181 209 2102
Fragments arranged by size:
2102 1179 209 181
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 587 2032 587
Size: 1445 2226
BstYI r'GATC_y
Cuts at: 1 176 696 1163 1186 1521 1767 2779 2865
Size: 175 520 467 23 335 246 1012 86
Cuts at: 2865 2877 3223 1
Size: 12 346 449
Fragments arranged by size:
1012 520 467 449 346 335 246 175
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 158 192 509 600 914 158
Size: 34 317 91 314 2915
Fragments arranged by size:
2915 317 314 91 34
DraI TTT'AAA
Cuts at: 670 670
Size: 3671
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 488 1256 1430 1821 1848 3193 488
Size: 768 174 391 27 1345 966
Fragments arranged by size:
1345 966 768 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 292 1694 1904 292
Size: 1402 210 2059
Fragments arranged by size:
2059 1402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 439 1496 1928 2675 2943 425
Size: 14 1057 432 747 268 1153
Fragments arranged by size:
1153 1057 747 432 268 14
EcoO109I rG'GnC_Cy
Cuts at: 543 657 810 543
Size: 114 153 3404
Fragments arranged by size:
3404 153 114
EcoRI G'AATT_C
Cuts at: 675 675
Size: 3671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 535 1105 1353 2375 3354 535
Size: 570 248 1022 979 852
Fragments arranged by size:
1022 979 852 570 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1513 1543 1545 1575 1513
Size: 30 2 30 3609
Fragments arranged by size:
3609 30 30 2
HincII GTy'rAC
Cuts at: 710 710
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2342 2526 2342
Size: 184 3487
MscI TGG'CCA
Cuts at: 1432 1432
Size: 3671
MslI CAynn'nnrTG
Cuts at: 1660 1787 1660
Size: 127 3544
MspA1I CmG'CkG
Cuts at: 514 627 782 1096 1456 2469 2714 514
Size: 113 155 314 360 1013 245 1471
Fragments arranged by size:
1471 1013 360 314 245 155 113
NaeI GCC'GGC
Cuts at: 492 1852 492
Size: 1360 2311
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 490 1850 490
Size: 1360 2311
NsiI A_TGCA'T
Cuts at: 133 133
Size: 3671
NspI r_CATG'y
Cuts at: 724 1224 2131 724
Size: 500 907 2264
Fragments arranged by size:
2264 907 500
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PflMI CCAn_nnn'nTGG
Cuts at: 546 546
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PspOMI G'GGCC_C
Cuts at: 656 656
Size: 3671
PstI C_TGCA'G
Cuts at: 409 718 1403 409
Size: 309 685 2677
Fragments arranged by size:
2677 685 309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 514 627 1096 1456 514
Size: 113 469 360 2729
Fragments arranged by size:
2729 469 360 113
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SfcI C'TryA_G
Cuts at: 33 405 714 1399 2392 2583 33
Size: 372 309 685 993 191 1121
Fragments arranged by size:
1121 993 685 372 309 191
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 523 693 523
Size: 170 3501
SmlI C'TyrA_G
Cuts at: 826 1741 2233 2495 826
Size: 915 492 262 2002
Fragments arranged by size:
2002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
SrfI GCCC'GGGC
Cuts at: 523 523
Size: 3671
StyI C'CwwG_G
Cuts at: 107 805 1159 107
Size: 698 354 2619
Fragments arranged by size:
2619 698 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 419 1254 1387 1846 2055 419
Size: 835 133 459 209 2035
Fragments arranged by size:
2035 835 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 164 196 263 295 325 357 3188 3220 164
Size: 32 67 32 30 32 2831 32 615
Fragments arranged by size:
2831 615 67 32 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 132 1166 114
Size: 18 1034 2619
Fragments arranged by size:
2619 1034 18
XmaI C'CCGG_G
Cuts at: 521 691 521
Size: 170 3501
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI