Restriction map for
pF1KB4415
(Circular) (Six-base) MAPSORT of: pF1KB4415.seq Check: 2705 from: 1 to: 3503
pF1KB4415 3503 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 541 541
Size: 3503
Acc65I G'GTAC_C
Cuts at: 519 519
Size: 3503
AclI AA'CG_TT
Cuts at: 3343 3343
Size: 3503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1760 2113 2775 1328
Size: 432 353 662 2056
Fragments arranged by size:
2056 662 432 353
AflIII A'CryG_T
Cuts at: 855 2655 2936 855
Size: 1800 281 1422
Fragments arranged by size:
1800 1422 281
AhdI GACnn_n'nnGTC
Cuts at: 473 473
Size: 3503
AlwNI CAG_nnn'CTG
Cuts at: 808 2246 808
Size: 1438 2065
ApaLI G'TGCA_C
Cuts at: 199 2341 199
Size: 2142 1361
ApoI r'AATT_y
Cuts at: 507 2856 3112 507
Size: 2349 256 898
Fragments arranged by size:
2349 898 256
AscI GG'CGCG_CC
Cuts at: 248 248
Size: 3503
AseI AT'TA_AT
Cuts at: 20 20
Size: 3503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
AvaI C'yCGr_G
Cuts at: 523 523
Size: 3503
BamHI G'GATC_C
Cuts at: 528 528
Size: 3503
BanI G'GyrC_C
Cuts at: 519 933 1181 1216 519
Size: 414 248 35 2806
Fragments arranged by size:
2806 414 248 35
BanII G_rGCy'C
Cuts at: 389 517 1547 389
Size: 128 1030 2345
Fragments arranged by size:
2345 1030 128
BbeI G_GCGC'C
Cuts at: 937 1185 937
Size: 248 3255
BbsI GAAGACnn'nnnn_
Cuts at: 340 472 340
Size: 132 3371
BciVI GTATCCnnnnn_n'
Cuts at: 1401 2457 3503 1401
Size: 1056 1046 1401
Fragments arranged by size:
1401 1056 1046
BclI T'GATC_A
Cuts at: 1023 1023
Size: 3503
BglII A'GATC_T
Cuts at: 1018 3055 1018
Size: 2037 1466
BlpI GC'TnA_GC
Cuts at: 615 615
Size: 3503
Bme1580I G_kGCm'C
Cuts at: 203 1128 1221 2345 203
Size: 925 93 1124 1361
Fragments arranged by size:
1361 1124 925 93
BmgBI CAC'GTC
Cuts at: 118 118
Size: 3503
BmrI ACTGGGnnnn_n'
Cuts at: 715 796 1131 3275 715
Size: 81 335 2144 943
Fragments arranged by size:
2144 943 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1870 1870
Size: 3503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 319 163
Size: 156 3347
Bpu10I CC'TnA_GC
Cuts at: 419 419
Size: 3503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1558 2272 2570 679
Size: 879 714 298 1612
Fragments arranged by size:
1612 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 3207 181
Size: 3026 477
BsaAI yAC'GTr
Cuts at: 856 856
Size: 3503
BsaBI GATnn'nnATC
Cuts at: 6 1017 3054 6
Size: 1011 2037 455
Fragments arranged by size:
2037 1011 455
BsaHI Gr'CG_yC
Cuts at: 428 934 1182 1884 3380 428
Size: 506 248 702 1496 551
Fragments arranged by size:
1496 702 551 506 248
BsaWI w'CCGG_w
Cuts at: 692 1213 1891 2302 2449 2723 692
Size: 521 678 411 147 274 1472
Fragments arranged by size:
1472 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 397
Size: 3503
BseYI C'CCAG_C
Cuts at: 928 1686 2351 2759 928
Size: 758 665 408 1672
Fragments arranged by size:
1672 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1045 1091 2321 104
Size: 941 46 1230 1286
Fragments arranged by size:
1286 1230 941 46
BsiHKAI G_wGCw'C
Cuts at: 203 389 517 1295 1485 2345 203
Size: 186 128 778 190 860 1361
Fragments arranged by size:
1361 860 778 190 186 128
Bsp1286I G_dGCh'C
Cuts at: 203 389 517 1128 1221 1295 1485 1547 2345
Size: 186 128 611 93 74 190 62 798
Cuts at: 2345 203
Size: 1361
Fragments arranged by size:
1361 798 611 190 186 128 93 74
62
BspHI T'CATG_A
Cuts at: 1935 1935
Size: 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 553 767 1069 1450 553
Size: 214 302 381 2606
Fragments arranged by size:
2606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 161 234 1795 1849 3333 3498 50
Size: 111 73 1561 54 1484 165 55
Fragments arranged by size:
1561 1484 165 111 73 55 54
BsrDI GCAATG_nn'
Cuts at: 413 1415 413
Size: 1002 2501
BsrFI r'CCGG_y
Cuts at: 252 307 1501 1682 252
Size: 55 1194 181 2073
Fragments arranged by size:
2073 1194 181 55
BssHII G'CGCG_C
Cuts at: 248 248
Size: 3503
BssSI C'ACGA_G
Cuts at: 1774 2482 1774
Size: 708 2795
BstAPI GCAn_nnn'nTGC
Cuts at: 207 880 207
Size: 673 2830
BstBI TT'CG_AA
Cuts at: 1864 1864
Size: 3503
BstEII G'GTnAC_C
Cuts at: 408 408
Size: 3503
BstYI r'GATC_y
Cuts at: 1 293 528 995 1018 1353 1599 1905 1917
Size: 292 235 467 23 335 246 306 12
Cuts at: 1917 2003 3055 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 449 335 306 292 246 235
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1453 1634 1797 1453
Size: 181 163 3159
Fragments arranged by size:
3159 181 163
BtsI GCAGTG_nn'
Cuts at: 746 746
Size: 3503
DraI TTT'AAA
Cuts at: 502 502
Size: 3503
DrdI GACnn_nn'nnGTC
Cuts at: 1209 2553 1209
Size: 1344 2159
EaeI y'GGCC_r
Cuts at: 305 365 1088 1262 1653 1680 3025 305
Size: 60 723 174 391 27 1345 783
Fragments arranged by size:
1345 783 723 391 174 60 27
EagI C'GGCC_G
Cuts at: 1088 1088
Size: 3503
EarI CTCTTCn'nnn_
Cuts at: 168 1526 1736 168
Size: 1358 210 1935
Fragments arranged by size:
1935 1358 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1711 2453 2599 3313 1711
Size: 742 146 714 1901
Fragments arranged by size:
1901 742 714 146
EcoICRI GAG'CTC
Cuts at: 387 515 387
Size: 128 3375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 319 1328 1760 2113 2775 163
Size: 156 1009 432 353 662 891
Fragments arranged by size:
1009 891 662 432 353 156
EcoO109I rG'GnC_Cy
Cuts at: 642 642
Size: 3503
EcoRI G'AATT_C
Cuts at: 507 507
Size: 3503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1522 1522
Size: 3503
FspI TGC'GCA
Cuts at: 1284 1284
Size: 3503
HaeII r_GCGC'y
Cuts at: 937 1185 2415 3186 937
Size: 248 1230 771 1254
Fragments arranged by size:
1254 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 402 434 459 491 1345 1375 1377 1407 402
Size: 32 25 32 854 30 2 30 2498
Fragments arranged by size:
2498 854 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 542 542
Size: 3503
KasI G'GCGC_C
Cuts at: 933 1181 933
Size: 248 3255
KpnI G_GTAC'C
Cuts at: 523 523
Size: 3503
MluI A'CGCG_T
Cuts at: 2936 2936
Size: 3503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2262 2446 2262
Size: 184 3319
MscI TGG'CCA
Cuts at: 367 1264 367
Size: 897 2606
MslI CAynn'nnrTG
Cuts at: 1492 1619 1492
Size: 127 3376
MspA1I CmG'CkG
Cuts at: 363 614 928 1288 2072 2317 363
Size: 251 314 360 784 245 1549
Fragments arranged by size:
1549 784 360 314 251 245
NaeI GCC'GGC
Cuts at: 309 1684 309
Size: 1375 2128
NarI GG'CG_CC
Cuts at: 934 1182 934
Size: 248 3255
NgoMIV G'CCGG_C
Cuts at: 307 1682 307
Size: 1375 2128
NruI TCG'CGA
Cuts at: 323 323
Size: 3503
NspI r_CATG'y
Cuts at: 556 1056 2659 556
Size: 500 1603 1400
Fragments arranged by size:
1603 1400 500
PciI A'CATG_T
Cuts at: 2655 2655
Size: 3503
PfoI T'CCnGG_A
Cuts at: 296 296
Size: 3503
PmeI GTTT'AAAC
Cuts at: 502 502
Size: 3503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1949 1981 1949
Size: 32 3471
PstI C_TGCA'G
Cuts at: 550 1235 550
Size: 685 2818
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3503
PvuII CAG'CTG
Cuts at: 363 928 1288 363
Size: 565 360 2578
Fragments arranged by size:
2578 565 360
RsrII CG'GwC_CG
Cuts at: 1698 1698
Size: 3503
SacI G_AGCT'C
Cuts at: 389 517 389
Size: 128 3375
SalI G'TCGA_C
Cuts at: 540 540
Size: 3503
SapI GCTCTTCn'nnn_
Cuts at: 168 1526 1736 168
Size: 1358 210 1935
Fragments arranged by size:
1935 1358 210
SbfI CC_TGCA'GG
Cuts at: 550 550
Size: 3503
ScaI AGT'ACT
Cuts at: 3020 3020
Size: 3503
SfcI C'TryA_G
Cuts at: 33 546 1231 2199 2390 33
Size: 513 685 968 191 1146
Fragments arranged by size:
1146 968 685 513 191
SfoI GGC'GCC
Cuts at: 935 1183 935
Size: 248 3255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3503
SgrAI Cr'CCGG_yG
Cuts at: 252 252
Size: 3503
SmaI CCC'GGG
Cuts at: 525 525
Size: 3503
SmlI C'TyrA_G
Cuts at: 658 1573 2287 2549 658
Size: 915 714 262 1612
Fragments arranged by size:
1612 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3503
SphI G_CATG'C
Cuts at: 556 1056 556
Size: 500 3003
StyI C'CwwG_G
Cuts at: 488 637 991 488
Size: 149 354 3000
Fragments arranged by size:
3000 354 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 1086 1219 1678 1887 484
Size: 602 133 459 209 2100
Fragments arranged by size:
2100 602 459 209 133
TatI w'GTAC_w
Cuts at: 3018 3018
Size: 3503
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3471
Tth111I GACn'n_nGTC
Cuts at: 1300 1300
Size: 3503
XbaI T'CTAG_A
Cuts at: 534 534
Size: 3503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 998 998
Size: 3503
XmaI C'CCGG_G
Cuts at: 523 523
Size: 3503
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI