Restriction map for
pF1KB4421
(Circular) (Six-base) MAPSORT of: pF1KB4421.seq Check: 2075 from: 1 to: 4688
pF1KB4421 4688 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1676 1676
Size: 4688
AccI GT'mk_AC
Cuts at: 1283 1726 1283
Size: 443 4245
Acc65I G'GTAC_C
Cuts at: 1704 1704
Size: 4688
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1253 2513 2945 3298 3960 784
Size: 469 1260 432 353 662 1512
Fragments arranged by size:
1512 1260 662 469 432 353
AflIII A'CryG_T
Cuts at: 1267 2040 3840 4121 1267
Size: 773 1800 281 1834
Fragments arranged by size:
1834 1800 773 281
AleI CACnn'nnGTG
Cuts at: 920 920
Size: 4688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1385 1417 1385
Size: 32 4656
AlwNI CAG_nnn'CTG
Cuts at: 1993 3431 1993
Size: 1438 3250
ApaLI G'TGCA_C
Cuts at: 3526 3526
Size: 4688
ApoI r'AATT_y
Cuts at: 319 1456 1692 4041 4297 319
Size: 1137 236 2349 256 710
Fragments arranged by size:
2349 1137 710 256 236
AseI AT'TA_AT
Cuts at: 20 952 20
Size: 932 3756
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 1708 1708
Size: 4688
BamHI G'GATC_C
Cuts at: 1713 1713
Size: 4688
BanI G'GyrC_C
Cuts at: 1587 1704 2118 2366 2401 1587
Size: 117 414 248 35 3874
Fragments arranged by size:
3874 414 248 117 35
BanII G_rGCy'C
Cuts at: 226 1208 1702 2732 226
Size: 982 494 1030 2182
Fragments arranged by size:
2182 1030 982 494
BbeI G_GCGC'C
Cuts at: 2122 2370 2122
Size: 248 4440
BbsI GAAGACnn'nnnn_
Cuts at: 689 689
Size: 4688
BbvCI CC'TCA_GC
Cuts at: 170 170
Size: 4688
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3642 4688 2586
Size: 1056 1046 2586
Fragments arranged by size:
2586 1056 1046
BclI T'GATC_A
Cuts at: 2208 2208
Size: 4688
BglII A'GATC_T
Cuts at: 1071 2203 4240 1071
Size: 1132 2037 1519
Fragments arranged by size:
2037 1519 1132
BlpI GC'TnA_GC
Cuts at: 1800 1800
Size: 4688
Bme1580I G_kGCm'C
Cuts at: 2313 2406 3530 2313
Size: 93 1124 3471
Fragments arranged by size:
3471 1124 93
BmgBI CAC'GTC
Cuts at: 1270 1270
Size: 4688
BmrI ACTGGGnnnn_n'
Cuts at: 464 998 1185 1444 1900 1981 2316 4460 464
Size: 534 187 259 456 81 335 2144 692
Fragments arranged by size:
2144 692 534 456 335 259 187 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 4688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 568 992 1157 259
Size: 309 424 165 3790
Fragments arranged by size:
3790 424 309 165
Bpu10I CC'TnA_GC
Cuts at: 170 170
Size: 4688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1864 2743 3457 3755 643
Size: 1221 879 714 298 1576
Fragments arranged by size:
1576 1221 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 752 4392 752
Size: 3640 1048
BsaAI yAC'GTr
Cuts at: 2041 2041
Size: 4688
BsaBI GATnn'nnATC
Cuts at: 6 2202 4239 6
Size: 2196 2037 455
Fragments arranged by size:
2196 2037 455
BsaHI Gr'CG_yC
Cuts at: 2119 2367 3069 4565 2119
Size: 248 702 1496 2242
Fragments arranged by size:
2242 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1877 2398 3076 3487 3634 3908 1877
Size: 521 678 411 147 274 2657
Fragments arranged by size:
2657 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 653 256
Size: 397 4291
BseYI C'CCAG_C
Cuts at: 141 2113 2871 3536 3944 141
Size: 1972 758 665 408 885
Fragments arranged by size:
1972 885 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2230 2276 3506 104
Size: 2126 46 1230 1286
Fragments arranged by size:
2126 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 978 1667 1702 2480 2670 3530 978
Size: 689 35 778 190 860 2136
Fragments arranged by size:
2136 860 778 689 190 35
Bsp1286I G_dGCh'C
Cuts at: 226 978 1208 1667 1702 2313 2406 2480 2670
Size: 752 230 459 35 611 93 74 190
Cuts at: 2670 2732 3530 226
Size: 62 798 1384
Fragments arranged by size:
1384 798 752 611 459 230 190 93
74 62 35
BspHI T'CATG_A
Cuts at: 840 3120 840
Size: 2280 2408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1676 1738 1952 2254 2635 1676
Size: 62 214 302 381 3729
Fragments arranged by size:
3729 381 302 214 62
BsrBI CCG'CTC
Cuts at: 50 2980 3034 4518 4683 50
Size: 2930 54 1484 165 55
Fragments arranged by size:
2930 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 497 2600 497
Size: 2103 2585
BsrFI r'CCGG_y
Cuts at: 2686 2867 2686
Size: 181 4507
BssSI C'ACGA_G
Cuts at: 2959 3667 2959
Size: 708 3980
BstAPI GCAn_nnn'nTGC
Cuts at: 2065 2065
Size: 4688
BstBI TT'CG_AA
Cuts at: 3049 3049
Size: 4688
BstXI CCAn_nnnn'nTGG
Cuts at: 723 723
Size: 4688
BstYI r'GATC_y
Cuts at: 1 1071 1713 2180 2203 2538 2784 3090 3102
Size: 1070 642 467 23 335 246 306 12
Cuts at: 3102 3188 4240 1
Size: 86 1052 449
Fragments arranged by size:
1070 1052 642 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 273 716 273
Size: 443 4245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 686 1931 686
Size: 1245 3443
DraI TTT'AAA
Cuts at: 1215 1687 1215
Size: 472 4216
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3738 2394
Size: 1344 3344
EaeI y'GGCC_r
Cuts at: 912 1493 1657 2273 2447 2838 2865 4210 912
Size: 581 164 616 174 391 27 1345 1390
Fragments arranged by size:
1390 1345 616 581 391 174 164 27
EagI C'GGCC_G
Cuts at: 2273 2273
Size: 4688
EarI CTCTTCn'nnn_
Cuts at: 1229 1476 2711 2921 1229
Size: 247 1235 210 2996
Fragments arranged by size:
2996 1235 247 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2896 3638 3784 4498 2896
Size: 742 146 714 3086
Fragments arranged by size:
3086 742 714 146
EcoICRI GAG'CTC
Cuts at: 1700 1700
Size: 4688
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 568 784 992 1157 1253 2513 2945 3298
Size: 309 216 208 165 96 1260 432 353
Cuts at: 3298 3960 259
Size: 662 987
Fragments arranged by size:
1260 987 662 432 353 309 216 208
165 96
EcoO109I rG'GnC_Cy
Cuts at: 1827 1827
Size: 4688
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 582 1404 1505 2707 582
Size: 822 101 1202 2563
Fragments arranged by size:
2563 1202 822 101
FspI TGC'GCA
Cuts at: 2469 2469
Size: 4688
HaeII r_GCGC'y
Cuts at: 2122 2370 3600 4371 2122
Size: 248 1230 771 2439
Fragments arranged by size:
2439 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 208 240 2530 2560 2562 2592 208
Size: 32 2290 30 2 30 2304
Fragments arranged by size:
2304 2290 32 30 30 2
HincII GTy'rAC
Cuts at: 1284 1727 1284
Size: 443 4245
HindIII A'AGCT_T
Cuts at: 1634 1634
Size: 4688
KasI G'GCGC_C
Cuts at: 2118 2366 2118
Size: 248 4440
KpnI G_GTAC'C
Cuts at: 1708 1708
Size: 4688
MfeI C'AATT_G
Cuts at: 1010 1010
Size: 4688
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 264 3447 3631 264
Size: 3183 184 1321
Fragments arranged by size:
3183 1321 184
MscI TGG'CCA
Cuts at: 914 2449 914
Size: 1535 3153
MslI CAynn'nnrTG
Cuts at: 475 920 2677 2804 475
Size: 445 1757 127 2359
Fragments arranged by size:
2359 1757 445 127
MspA1I CmG'CkG
Cuts at: 1799 2113 2473 3257 3502 1799
Size: 314 360 784 245 2985
Fragments arranged by size:
2985 784 360 314 245
NaeI GCC'GGC
Cuts at: 2869 2869
Size: 4688
NarI GG'CG_CC
Cuts at: 2119 2367 2119
Size: 248 4440
NcoI C'CATG_G
Cuts at: 273 716 273
Size: 443 4245
NdeI CA'TA_TG
Cuts at: 291 1344 291
Size: 1053 3635
NgoMIV G'CCGG_C
Cuts at: 2867 2867
Size: 4688
NspI r_CATG'y
Cuts at: 966 1741 2241 3844 966
Size: 775 500 1603 1810
Fragments arranged by size:
1810 1603 775 500
PciI A'CATG_T
Cuts at: 3840 3840
Size: 4688
PflMI CCAn_nnn'nTGG
Cuts at: 988 988
Size: 4688
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 4656
PshAI GACnn'nnGTC
Cuts at: 1279 1279
Size: 4688
PstI C_TGCA'G
Cuts at: 1735 2420 1735
Size: 685 4003
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 2113 2473 2113
Size: 360 4328
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 1702 1702
Size: 4688
SalI G'TCGA_C
Cuts at: 1282 1725 1282
Size: 443 4245
SapI GCTCTTCn'nnn_
Cuts at: 2711 2921 2711
Size: 210 4478
SbfI CC_TGCA'GG
Cuts at: 1735 1735
Size: 4688
ScaI AGT'ACT
Cuts at: 4205 4205
Size: 4688
SfcI C'TryA_G
Cuts at: 33 753 1731 2416 3384 3575 33
Size: 720 978 685 968 191 1146
Fragments arranged by size:
1146 978 968 720 685 191
SfoI GGC'GCC
Cuts at: 2120 2368 2120
Size: 248 4440
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4688
SmlI C'TyrA_G
Cuts at: 622 1843 2758 3472 3734 622
Size: 1221 915 714 262 1576
Fragments arranged by size:
1576 1221 915 714 262
SpeI A'CTAG_T
Cuts at: 67 337 67
Size: 270 4418
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
SspI AAT'ATT
Cuts at: 464 543 674 464
Size: 79 131 4478
Fragments arranged by size:
4478 131 79
StyI C'CwwG_G
Cuts at: 273 716 792 1822 2176 273
Size: 443 76 1030 354 2785
Fragments arranged by size:
2785 1030 443 354 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2271 2404 2863 3072 2271
Size: 133 459 209 3887
Fragments arranged by size:
3887 459 209 133
TatI w'GTAC_w
Cuts at: 1359 4203 1359
Size: 2844 1844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1491 1523 4205 4237 1491
Size: 32 2682 32 1942
Fragments arranged by size:
2682 1942 32 32
Tth111I GACn'n_nGTC
Cuts at: 2485 2485
Size: 4688
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 280 2183 280
Size: 1903 2785
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4688
XmnI GAAnn'nnTTC
Cuts at: 836 1184 836
Size: 348 4340
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI