Restriction map for
pF1KB4445
(Circular) (Six-base) MAPSORT of: pF1KB4445.seq Check: 7912 from: 1 to: 3560
pF1KB4445 3560 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 598 598
Size: 3560
Acc65I G'GTAC_C
Cuts at: 576 576
Size: 3560
AclI AA'CG_TT
Cuts at: 3400 3400
Size: 3560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1385 1817 2170 2832 263
Size: 1122 432 353 662 991
Fragments arranged by size:
1122 991 662 432 353
AflIII A'CryG_T
Cuts at: 912 2712 2993 912
Size: 1800 281 1479
Fragments arranged by size:
1800 1479 281
AlwNI CAG_nnn'CTG
Cuts at: 865 2303 865
Size: 1438 2122
ApaLI G'TGCA_C
Cuts at: 2398 2398
Size: 3560
ApoI r'AATT_y
Cuts at: 564 2913 3169 564
Size: 2349 256 955
Fragments arranged by size:
2349 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
AvaI C'yCGr_G
Cuts at: 580 580
Size: 3560
BamHI G'GATC_C
Cuts at: 585 585
Size: 3560
BanI G'GyrC_C
Cuts at: 576 990 1238 1273 576
Size: 414 248 35 2863
Fragments arranged by size:
2863 414 248 35
BanII G_rGCy'C
Cuts at: 574 1604 574
Size: 1030 2530
BbeI G_GCGC'C
Cuts at: 994 1242 994
Size: 248 3312
BciVI GTATCCnnnnn_n'
Cuts at: 1458 2514 3560 1458
Size: 1056 1046 1458
Fragments arranged by size:
1458 1056 1046
BclI T'GATC_A
Cuts at: 263 482 1080 263
Size: 219 598 2743
Fragments arranged by size:
2743 598 219
BglII A'GATC_T
Cuts at: 1075 3112 1075
Size: 2037 1523
BlpI GC'TnA_GC
Cuts at: 672 672
Size: 3560
Bme1580I G_kGCm'C
Cuts at: 1185 1278 2402 1185
Size: 93 1124 2343
Fragments arranged by size:
2343 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 772 853 1188 3332 772
Size: 81 335 2144 1000
Fragments arranged by size:
2144 1000 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 174 1927 174
Size: 1753 1807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1615 2329 2627 736
Size: 879 714 298 1669
Fragments arranged by size:
1669 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3264 3264
Size: 3560
BsaAI yAC'GTr
Cuts at: 432 913 432
Size: 481 3079
BsaBI GATnn'nnATC
Cuts at: 6 1074 3111 6
Size: 1068 2037 455
Fragments arranged by size:
2037 1068 455
BsaHI Gr'CG_yC
Cuts at: 991 1239 1941 3437 991
Size: 248 702 1496 1114
Fragments arranged by size:
1496 1114 702 248
BsaWI w'CCGG_w
Cuts at: 749 1270 1948 2359 2506 2780 749
Size: 521 678 411 147 274 1529
Fragments arranged by size:
1529 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 370 373 481 142
Size: 228 3 108 3221
Fragments arranged by size:
3221 228 108 3
BseYI C'CCAG_C
Cuts at: 985 1743 2408 2816 985
Size: 758 665 408 1729
Fragments arranged by size:
1729 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1102 1148 2378 104
Size: 998 46 1230 1286
Fragments arranged by size:
1286 1230 998 46
BsiHKAI G_wGCw'C
Cuts at: 574 1352 1542 2402 574
Size: 778 190 860 1732
Fragments arranged by size:
1732 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 574 1185 1278 1352 1542 1604 2402 574
Size: 611 93 74 190 62 798 1732
Fragments arranged by size:
1732 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1992 1992
Size: 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 610 824 1126 1507 610
Size: 214 302 381 2663
Fragments arranged by size:
2663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1852 1906 3390 3555 50
Size: 1802 54 1484 165 55
Fragments arranged by size:
1802 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1472 1472
Size: 3560
BsrFI r'CCGG_y
Cuts at: 1558 1739 1558
Size: 181 3379
BsrGI T'GTAC_A
Cuts at: 534 534
Size: 3560
BssSI C'ACGA_G
Cuts at: 1831 2539 1831
Size: 708 2852
BstAPI GCAn_nnn'nTGC
Cuts at: 937 937
Size: 3560
BstBI TT'CG_AA
Cuts at: 1921 1921
Size: 3560
BstXI CCAn_nnnn'nTGG
Cuts at: 204 204
Size: 3560
BstYI r'GATC_y
Cuts at: 1 220 362 585 1052 1075 1410 1656 1962
Size: 219 142 223 467 23 335 246 306
Cuts at: 1962 1974 2060 3112 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 335 306 246 223 219
142 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1510 1691 1854 1510
Size: 181 163 3216
Fragments arranged by size:
3216 181 163
BtsI GCAGTG_nn'
Cuts at: 803 803
Size: 3560
ClaI AT'CG_AT
Cuts at: 500 500
Size: 3560
DraI TTT'AAA
Cuts at: 559 559
Size: 3560
DrdI GACnn_nn'nnGTC
Cuts at: 1266 2610 1266
Size: 1344 2216
EaeI y'GGCC_r
Cuts at: 326 510 1145 1319 1710 1737 3082 326
Size: 184 635 174 391 27 1345 804
Fragments arranged by size:
1345 804 635 391 184 174 27
EagI C'GGCC_G
Cuts at: 1145 1145
Size: 3560
EarI CTCTTCn'nnn_
Cuts at: 521 1583 1793 521
Size: 1062 210 2288
Fragments arranged by size:
2288 1062 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1768 2510 2656 3370 1768
Size: 742 146 714 1958
Fragments arranged by size:
1958 742 714 146
EcoICRI GAG'CTC
Cuts at: 572 572
Size: 3560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1385 1817 2170 2832 263
Size: 1122 432 353 662 991
Fragments arranged by size:
1122 991 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 699 699
Size: 3560
EcoRI G'AATT_C
Cuts at: 564 564
Size: 3560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 1579 171
Size: 1408 2152
FspI TGC'GCA
Cuts at: 1341 1341
Size: 3560
HaeII r_GCGC'y
Cuts at: 416 994 1242 2472 3243 416
Size: 578 248 1230 771 733
Fragments arranged by size:
1230 771 733 578 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1402 1432 1434 1464 1402
Size: 30 2 30 3498
Fragments arranged by size:
3498 30 30 2
HincII GTy'rAC
Cuts at: 599 599
Size: 3560
KasI G'GCGC_C
Cuts at: 990 1238 990
Size: 248 3312
KpnI G_GTAC'C
Cuts at: 580 580
Size: 3560
MluI A'CGCG_T
Cuts at: 2993 2993
Size: 3560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2319 2503 2319
Size: 184 3376
MscI TGG'CCA
Cuts at: 328 512 1321 328
Size: 184 809 2567
Fragments arranged by size:
2567 809 184
MslI CAynn'nnrTG
Cuts at: 1549 1676 1549
Size: 127 3433
MspA1I CmG'CkG
Cuts at: 120 671 985 1345 2129 2374 120
Size: 551 314 360 784 245 1306
Fragments arranged by size:
1306 784 551 360 314 245
NaeI GCC'GGC
Cuts at: 1741 1741
Size: 3560
NarI GG'CG_CC
Cuts at: 991 1239 991
Size: 248 3312
NcoI C'CATG_G
Cuts at: 107 107
Size: 3560
NgoMIV G'CCGG_C
Cuts at: 1739 1739
Size: 3560
NspI r_CATG'y
Cuts at: 613 1113 2716 613
Size: 500 1603 1457
Fragments arranged by size:
1603 1457 500
PasI CC'CwG_GG
Cuts at: 225 225
Size: 3560
PciI A'CATG_T
Cuts at: 2712 2712
Size: 3560
PfoI T'CCnGG_A
Cuts at: 305 305
Size: 3560
PmeI GTTT'AAAC
Cuts at: 559 559
Size: 3560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2006 2038 2006
Size: 32 3528
PstI C_TGCA'G
Cuts at: 257 607 1292 257
Size: 350 685 2525
Fragments arranged by size:
2525 685 350
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3560
PvuII CAG'CTG
Cuts at: 120 985 1345 120
Size: 865 360 2335
Fragments arranged by size:
2335 865 360
RsrII CG'GwC_CG
Cuts at: 1755 1755
Size: 3560
SacI G_AGCT'C
Cuts at: 574 574
Size: 3560
SalI G'TCGA_C
Cuts at: 597 597
Size: 3560
SapI GCTCTTCn'nnn_
Cuts at: 1583 1793 1583
Size: 210 3350
SbfI CC_TGCA'GG
Cuts at: 607 607
Size: 3560
ScaI AGT'ACT
Cuts at: 3077 3077
Size: 3560
SfcI C'TryA_G
Cuts at: 33 253 603 1288 2256 2447 33
Size: 220 350 685 968 191 1146
Fragments arranged by size:
1146 968 685 350 220 191
SfoI GGC'GCC
Cuts at: 992 1240 992
Size: 248 3312
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3560
SmaI CCC'GGG
Cuts at: 582 582
Size: 3560
SmlI C'TyrA_G
Cuts at: 715 1630 2344 2606 715
Size: 915 714 262 1669
Fragments arranged by size:
1669 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3560
SphI G_CATG'C
Cuts at: 613 1113 613
Size: 500 3060
StuI AGG'CCT
Cuts at: 154 154
Size: 3560
StyI C'CwwG_G
Cuts at: 107 197 694 1048 107
Size: 90 497 354 2619
Fragments arranged by size:
2619 497 354 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 475 1143 1276 1735 1944 475
Size: 668 133 459 209 2091
Fragments arranged by size:
2091 668 459 209 133
TatI w'GTAC_w
Cuts at: 534 3075 534
Size: 2541 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 3528
Tth111I GACn'n_nGTC
Cuts at: 1357 1357
Size: 3560
XbaI T'CTAG_A
Cuts at: 591 591
Size: 3560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 1055 201
Size: 854 2706
XmaI C'CCGG_G
Cuts at: 580 580
Size: 3560
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI