Restriction map for
pF1KB4450
(Circular) (Six-base) MAPSORT of: pF1KB4450.seq Check: 4003 from: 1 to: 4253
pF1KB4450 4253 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1000 1085 1000
Size: 85 4168
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 2510 2863 3525 2078
Size: 432 353 662 2806
Fragments arranged by size:
2806 662 432 353
AflIII A'CryG_T
Cuts at: 212 371 443 1605 3405 3686 212
Size: 159 72 1162 1800 281 779
Fragments arranged by size:
1800 1162 779 281 159 72
AhdI GACnn_n'nnGTC
Cuts at: 153 153
Size: 4253
AleI CACnn'nnGTG
Cuts at: 640 727 640
Size: 87 4166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 952 1090 952
Size: 138 4115
AlwNI CAG_nnn'CTG
Cuts at: 1558 2996 1558
Size: 1438 2815
ApaI G_GGCC'C
Cuts at: 195 1169 195
Size: 974 3279
ApaLI G'TGCA_C
Cuts at: 3091 3091
Size: 4253
ApoI r'AATT_y
Cuts at: 1257 3606 3862 1257
Size: 2349 256 1648
Fragments arranged by size:
2349 1648 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 186 1273 186
Size: 1087 3166
BamHI G'GATC_C
Cuts at: 1278 1278
Size: 4253
BanI G'GyrC_C
Cuts at: 232 741 836 1105 1269 1683 1931 1966 232
Size: 509 95 269 164 414 248 35 2519
Fragments arranged by size:
2519 509 414 269 248 164 95 35
BanII G_rGCy'C
Cuts at: 195 685 1169 1267 2297 195
Size: 490 484 98 1030 2151
Fragments arranged by size:
2151 1030 490 484 98
BbeI G_GCGC'C
Cuts at: 840 1687 1935 840
Size: 847 248 3158
Fragments arranged by size:
3158 847 248
BbsI GAAGACnn'nnnn_
Cuts at: 213 450 916 213
Size: 237 466 3550
Fragments arranged by size:
3550 466 237
BbvCI CC'TCA_GC
Cuts at: 475 526 475
Size: 51 4202
BciVI GTATCCnnnnn_n'
Cuts at: 2151 3207 4253 2151
Size: 1056 1046 2151
Fragments arranged by size:
2151 1056 1046
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglI GCCn_nnn'nGGC
Cuts at: 470 586 470
Size: 116 4137
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 294 418 847 1365 294
Size: 124 429 518 3182
Fragments arranged by size:
3182 518 429 124
Bme1580I G_kGCm'C
Cuts at: 195 235 746 1157 1169 1878 1971 3095 195
Size: 40 511 411 12 709 93 1124 1353
Fragments arranged by size:
1353 1124 709 511 411 93 40 12
BmrI ACTGGGnnnn_n'
Cuts at: 1465 1546 1881 4025 1465
Size: 81 335 2144 1693
Fragments arranged by size:
2144 1693 335 81
BmtI G_CTAG'C
Cuts at: 317 317
Size: 4253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
Bpu10I CC'TnA_GC
Cuts at: 475 526 475
Size: 51 4202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 3022 3320 1429
Size: 879 714 298 2362
Fragments arranged by size:
2362 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 720 1225 3957 720
Size: 505 2732 1016
Fragments arranged by size:
2732 1016 505
BsaAI yAC'GTr
Cuts at: 374 1606 374
Size: 1232 3021
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 837 1684 1932 2634 4130 837
Size: 847 248 702 1496 960
Fragments arranged by size:
1496 960 847 702 248
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 3052 3199 3473 1442
Size: 521 678 411 147 274 2222
Fragments arranged by size:
2222 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 924 1678 2436 3101 3509 924
Size: 754 758 665 408 1668
Fragments arranged by size:
1668 758 754 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 922 972 1050 664
Size: 258 50 78 3867
Fragments arranged by size:
3867 258 78 50
BsiEI CG_ry'CG
Cuts at: 104 486 1795 1841 3071 104
Size: 382 1309 46 1230 1286
Fragments arranged by size:
1309 1286 1230 382 46
BsiHKAI G_wGCw'C
Cuts at: 257 685 1267 2045 2235 3095 257
Size: 428 582 778 190 860 1415
Fragments arranged by size:
1415 860 778 582 428 190
Bsp1286I G_dGCh'C
Cuts at: 195 235 257 685 746 1157 1169 1267 1878
Size: 40 22 428 61 411 12 98 611
Cuts at: 1878 1971 2045 2235 2297 3095 195
Size: 93 74 190 62 798 1353
Fragments arranged by size:
1353 798 611 428 411 190 98 93
74 62 61 40 22 12
BspHI T'CATG_A
Cuts at: 968 2685 968
Size: 1717 2536
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1000 1085 1303 1517 1819 2200 1000
Size: 85 218 214 302 381 3053
Fragments arranged by size:
3053 381 302 218 214 85
BsrBI CCG'CTC
Cuts at: 50 2545 2599 4083 4248 50
Size: 2495 54 1484 165 55
Fragments arranged by size:
2495 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 308 2165 308
Size: 1857 2396
BsrFI r'CCGG_y
Cuts at: 469 839 2251 2432 469
Size: 370 1412 181 2290
Fragments arranged by size:
2290 1412 370 181
BsrGI T'GTAC_A
Cuts at: 917 917
Size: 4253
BssSI C'ACGA_G
Cuts at: 1204 2524 3232 1204
Size: 1320 708 2225
Fragments arranged by size:
2225 1320 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstEII G'GTnAC_C
Cuts at: 870 870
Size: 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 729 729
Size: 4253
BstYI r'GATC_y
Cuts at: 1 1278 1745 1768 2103 2349 2655 2667 2753
Size: 1277 467 23 335 246 306 12 86
Cuts at: 2753 3805 1
Size: 1052 449
Fragments arranged by size:
1277 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 236 548 554 665 728 107
Size: 129 312 6 111 63 3632
Fragments arranged by size:
3632 312 129 111 63 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 762 774 2203 2384 2547 762
Size: 12 1429 181 163 2468
Fragments arranged by size:
2468 1429 181 163 12
BtsI GCAGTG_nn'
Cuts at: 652 1128 1496 652
Size: 476 368 3409
Fragments arranged by size:
3409 476 368
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DraIII CAC_nnn'GTG
Cuts at: 906 906
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 3303 1959
Size: 1344 2909
EaeI y'GGCC_r
Cuts at: 494 512 551 575 587 671 795 1086 1838
Size: 18 39 24 12 84 124 291 752
Cuts at: 1838 2012 2403 2430 3775 494
Size: 174 391 27 1345 972
Fragments arranged by size:
1345 972 752 391 291 174 124 84
39 27 24 18 12
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 280 967 1152 2276 2486 280
Size: 687 185 1124 210 2047
Fragments arranged by size:
2047 1124 687 210 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1045 2461 3203 3349 4063 1045
Size: 1416 742 146 714 1235
Fragments arranged by size:
1416 1235 742 714 146
EcoICRI GAG'CTC
Cuts at: 683 1265 683
Size: 582 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 2510 2863 3525 2078
Size: 432 353 662 2806
Fragments arranged by size:
2806 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 191 192 401 822 1165 1392 191
Size: 1 209 421 343 227 3052
Fragments arranged by size:
3052 421 343 227 209 1
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
EcoRV GAT'ATC
Cuts at: 303 303
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 1101 2034 1101
Size: 933 3320
HaeII r_GCGC'y
Cuts at: 294 840 1687 1935 3165 3936 294
Size: 546 847 248 1230 771 611
Fragments arranged by size:
1230 847 771 611 546 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 166 171 198 2095 2125 2127 2157 139
Size: 27 5 27 1897 30 2 30 2235
Fragments arranged by size:
2235 1897 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 441 1008 1245 1292 441
Size: 567 237 47 3402
Fragments arranged by size:
3402 567 237 47
KasI G'GCGC_C
Cuts at: 836 1683 1931 836
Size: 847 248 3158
Fragments arranged by size:
3158 847 248
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3012 3196 3012
Size: 184 4069
MscI TGG'CCA
Cuts at: 496 514 553 589 673 797 1088 2014 496
Size: 18 39 36 84 124 291 926 2735
Fragments arranged by size:
2735 926 291 124 84 39 36 18
MslI CAynn'nnrTG
Cuts at: 211 640 727 754 856 859 1072 2242 2369
Size: 429 87 27 102 3 213 1170 127
Cuts at: 2369 211
Size: 2095
Fragments arranged by size:
2095 1170 429 213 127 102 87 27
3
MspA1I CmG'CkG
Cuts at: 298 542 1013 1364 1678 2038 2822 3067 298
Size: 244 471 351 314 360 784 245 1484
Fragments arranged by size:
1484 784 471 360 351 314 245 244
NaeI GCC'GGC
Cuts at: 841 2434 841
Size: 1593 2660
NarI GG'CG_CC
Cuts at: 837 1684 1932 837
Size: 847 248 3158
Fragments arranged by size:
3158 847 248
NcoI C'CATG_G
Cuts at: 107 665 107
Size: 558 3695
NgoMIV G'CCGG_C
Cuts at: 839 2432 839
Size: 1593 2660
NheI G'CTAG_C
Cuts at: 313 313
Size: 4253
NspI r_CATG'y
Cuts at: 216 447 924 1306 1806 3409 216
Size: 231 477 382 500 1603 1060
Fragments arranged by size:
1603 1060 500 477 382 231
PciI A'CATG_T
Cuts at: 212 443 3405 212
Size: 231 2962 1060
Fragments arranged by size:
2962 1060 231
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 4221
PpuMI rG'GwC_Cy
Cuts at: 401 401
Size: 4253
PspOMI G'GGCC_C
Cuts at: 191 1165 191
Size: 974 3279
PstI C_TGCA'G
Cuts at: 1300 1985 1300
Size: 685 3568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 298 1013 1678 2038 298
Size: 715 665 360 2513
Fragments arranged by size:
2513 715 665 360
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 685 1267 685
Size: 582 3671
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SexAI A'CCwGG_T
Cuts at: 902 902
Size: 4253
SfcI C'TryA_G
Cuts at: 33 1221 1296 1981 2949 3140 33
Size: 1188 75 685 968 191 1146
Fragments arranged by size:
1188 1146 968 685 191 75
SfoI GGC'GCC
Cuts at: 838 1685 1933 838
Size: 847 248 3158
Fragments arranged by size:
3158 847 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 1408 2323 3037 3299 1408
Size: 915 714 262 2362
Fragments arranged by size:
2362 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StuI AGG'CCT
Cuts at: 1063 1063
Size: 4253
StyI C'CwwG_G
Cuts at: 107 665 708 1387 1741 107
Size: 558 43 679 354 2619
Fragments arranged by size:
2619 679 558 354 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 558 1836 1969 2428 2637 558
Size: 1278 133 459 209 2174
Fragments arranged by size:
2174 1278 459 209 133
TatI w'GTAC_w
Cuts at: 167 917 3768 167
Size: 750 2851 652
Fragments arranged by size:
2851 750 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1195 1227 3770 3802 1195
Size: 32 2543 32 1646
Fragments arranged by size:
2543 1646 32 32
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 213 597 621 790 1120 1748 213
Size: 384 24 169 330 628 2718
Fragments arranged by size:
2718 628 384 330 169 24
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
XmnI GAAnn'nnTTC
Cuts at: 976 976
Size: 4253
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BpmI BsaXI BseRI BsiWI BsmI
BsmBI BspEI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI