Restriction map for
pF1KB4456
(Circular) (Six-base) MAPSORT of: pF1KB4456.seq Check: 9850 from: 1 to: 5489
pF1KB4456 5489 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 381 796 1129 1365 1861 381
Size: 415 333 236 496 4009
Fragments arranged by size:
4009 496 415 333 236
AatII G_ACGT'C
Cuts at: 2306 2306
Size: 5489
AccI GT'mk_AC
Cuts at: 2527 2527
Size: 5489
Acc65I G'GTAC_C
Cuts at: 2505 2505
Size: 5489
AclI AA'CG_TT
Cuts at: 5329 5329
Size: 5489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 694 2180 3314 3746 4099 4761 453
Size: 241 1486 1134 432 353 662 1181
Fragments arranged by size:
1486 1181 1134 662 432 353 241
AfeI AGC'GCT
Cuts at: 850 850
Size: 5489
AflIII A'CryG_T
Cuts at: 1394 2841 4641 4922 1394
Size: 1447 1800 281 1961
Fragments arranged by size:
1961 1800 1447 281
AhdI GACnn_n'nnGTC
Cuts at: 1677 1893 1677
Size: 216 5273
AleI CACnn'nnGTG
Cuts at: 470 541 470
Size: 71 5418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 739
Size: 5489
AlwNI CAG_nnn'CTG
Cuts at: 387 396 643 2794 4232 387
Size: 9 247 2151 1438 1644
Fragments arranged by size:
2151 1644 1438 247 9
ApaI G_GGCC'C
Cuts at: 229 320 380 558 1006 1456 1521 1760 229
Size: 91 60 178 448 450 65 239 3958
Fragments arranged by size:
3958 450 448 239 178 91 65 60
ApaLI G'TGCA_C
Cuts at: 1339 2035 4327 1339
Size: 696 2292 2501
Fragments arranged by size:
2501 2292 696
ApoI r'AATT_y
Cuts at: 2493 4842 5098 2493
Size: 2349 256 2884
Fragments arranged by size:
2884 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5489
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5489
AvaI C'yCGr_G
Cuts at: 222 298 498 574 1480 1514 1827 1864 1916
Size: 76 200 76 906 34 313 37 52
Cuts at: 1916 2027 2353 2509 222
Size: 111 326 156 3202
Fragments arranged by size:
3202 906 326 313 200 156 111 76
76 52 37 34
BamHI G'GATC_C
Cuts at: 2514 2514
Size: 5489
BanI G'GyrC_C
Cuts at: 112 163 351 762 1738 2332 2505 2919 3167
Size: 51 188 411 976 594 173 414 248
Cuts at: 3167 3202 112
Size: 35 2399
Fragments arranged by size:
2399 976 594 414 411 248 188 173
51 35
BanII G_rGCy'C
Cuts at: 229 236 320 380 558 1006 1330 1408 1456
Size: 7 84 60 178 448 324 78 48
Cuts at: 1456 1481 1521 1760 2117 2148 2450 2503 3533
Size: 25 40 239 357 31 302 53 1030
Cuts at: 3533 229
Size: 2185
Fragments arranged by size:
2185 1030 448 357 324 302 239 178
84 78 60 53 48 40 31 25
7
BbeI G_GCGC'C
Cuts at: 116 167 1742 2336 2923 3171 116
Size: 51 1575 594 587 248 2434
Fragments arranged by size:
2434 1575 594 587 248 51
BbsI GAAGACnn'nnnn_
Cuts at: 249 518 2065 249
Size: 269 1547 3673
Fragments arranged by size:
3673 1547 269
BbvCI CC'TCA_GC
Cuts at: 530 1237 530
Size: 707 4782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1471 1505 1471
Size: 34 5455
BciVI GTATCCnnnnn_n'
Cuts at: 3387 4443 5489 3387
Size: 1056 1046 3387
Fragments arranged by size:
3387 1056 1046
BclI T'GATC_A
Cuts at: 2214 3009 2214
Size: 795 4694
BglI GCCn_nnn'nGGC
Cuts at: 157 384 607 1010 1130 157
Size: 227 223 403 120 4516
Fragments arranged by size:
4516 403 227 223 120
BglII A'GATC_T
Cuts at: 1346 2097 3004 5041 1346
Size: 751 907 2037 1794
Fragments arranged by size:
2037 1794 907 751
BlpI GC'TnA_GC
Cuts at: 283 2601 283
Size: 2318 3171
Bme1580I G_kGCm'C
Cuts at: 229 320 356 380 558 767 1006 1343 1456
Size: 91 36 24 178 209 239 337 113
Cuts at: 1456 1521 1760 2039 3114 3207 4331 229
Size: 65 239 279 1075 93 1124 1387
Fragments arranged by size:
1387 1124 1075 337 279 239 239 209
178 113 93 91 65 36 24
BmgBI CAC'GTC
Cuts at: 840 918 2034 2457 840
Size: 78 1116 423 3872
Fragments arranged by size:
3872 1116 423 78
BmrI ACTGGGnnnn_n'
Cuts at: 2701 2782 3117 5261 2701
Size: 81 335 2144 2929
Fragments arranged by size:
2929 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1095 2365 3856 1095
Size: 1270 1491 2728
Fragments arranged by size:
2728 1491 1270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1405 2362 865
Size: 540 957 3992
Fragments arranged by size:
3992 957 540
Bpu10I CC'TnA_GC
Cuts at: 530 1237 530
Size: 707 4782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1018 1348 2665 3544 4258 4556 955
Size: 63 330 1317 879 714 298 1888
Fragments arranged by size:
1888 1317 879 714 330 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 5193 5193
Size: 5489
BsaAI yAC'GTr
Cuts at: 978 1767 2842 978
Size: 789 1075 3625
Fragments arranged by size:
3625 1075 789
BsaBI GATnn'nnATC
Cuts at: 6 2005 3003 5040 6
Size: 1999 998 2037 455
Fragments arranged by size:
2037 1999 998 455
BsaHI Gr'CG_yC
Cuts at: 113 164 1106 1739 2303 2333 2920 3168 3870
Size: 51 942 633 564 30 587 248 702
Cuts at: 3870 5366 113
Size: 1496 236
Fragments arranged by size:
1496 942 702 633 587 564 248 236
51 30
BsaWI w'CCGG_w
Cuts at: 2678 3199 3877 4288 4435 4709 2678
Size: 521 678 411 147 274 3458
Fragments arranged by size:
3458 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 400 430 474 504 790 820 1333 1363 1921
Size: 30 44 30 286 30 513 30 558
Cuts at: 1921 1951 400
Size: 30 3938
Fragments arranged by size:
3938 558 513 286 44 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 1201 1204 1882 2156 2374 159
Size: 1042 3 678 274 218 3274
Fragments arranged by size:
3274 1042 678 274 218 3
BseYI C'CCAG_C
Cuts at: 380 636 906 1734 2107 2372 2435 2914 3672
Size: 256 270 828 373 265 63 479 758
Cuts at: 3672 4337 4745 380
Size: 665 408 1124
Fragments arranged by size:
1124 828 758 665 479 408 373 270
265 256 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1728 2179 1728
Size: 451 5038
BsiEI CG_ry'CG
Cuts at: 104 611 3031 3077 4307 104
Size: 507 2420 46 1230 1286
Fragments arranged by size:
2420 1286 1230 507 46
BsiHKAI G_wGCw'C
Cuts at: 704 1034 1343 1408 1481 2039 2148 2503 3281
Size: 330 309 65 73 558 109 355 778
Cuts at: 3281 3471 4331 704
Size: 190 860 1862
Fragments arranged by size:
1862 860 778 558 355 330 309 190
109 73 65
BsmI GAATG_Cn'
Cuts at: 405 405
Size: 5489
Bsp1286I G_dGCh'C
Cuts at: 229 236 320 356 380 558 704 767 1006
Size: 7 84 36 24 178 146 63 239
Cuts at: 1006 1034 1330 1343 1408 1456 1481 1521 1760
Size: 28 296 13 65 48 25 40 239
Cuts at: 1760 2039 2117 2148 2450 2503 3114 3207 3281
Size: 279 78 31 302 53 611 93 74
Cuts at: 3281 3471 3533 4331 229
Size: 190 62 798 1387
Fragments arranged by size:
1387 798 611 302 296 279 239 239
190 178 146 93 84 78 74 65
63 62 53 48 40 36 31 28
25 24 13 7
BspHI T'CATG_A
Cuts at: 3921 3921
Size: 5489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 381 796 1129 1365 1739 1805 1861 2424 2539
Size: 415 333 236 374 66 56 563 115
Cuts at: 2539 2753 3055 3436 381
Size: 214 302 381 2434
Fragments arranged by size:
2434 563 415 381 374 333 302 236
214 115 66 56
BsrBI CCG'CTC
Cuts at: 50 211 579 1362 3781 3835 5319 5484 50
Size: 161 368 783 2419 54 1484 165 55
Fragments arranged by size:
2419 1484 783 368 165 161 55 54
BsrDI GCAATG_nn'
Cuts at: 1980 3401 1980
Size: 1421 4068
BsrFI r'CCGG_y
Cuts at: 160 434 606 860 885 3487 3668 160
Size: 274 172 254 25 2602 181 1981
Fragments arranged by size:
2602 1981 274 254 181 172 25
BsrGI T'GTAC_A
Cuts at: 938 2208 938
Size: 1270 4219
BssSI C'ACGA_G
Cuts at: 409 3760 4468 409
Size: 3351 708 1430
Fragments arranged by size:
3351 1430 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2866 2866
Size: 5489
BstBI TT'CG_AA
Cuts at: 3850 3850
Size: 5489
BstEII G'GTnAC_C
Cuts at: 1792 1983 1792
Size: 191 5298
BstYI r'GATC_y
Cuts at: 1 1346 2097 2514 2981 3004 3339 3585 3891
Size: 1345 751 417 467 23 335 246 306
Cuts at: 3891 3903 3989 5041 1
Size: 12 86 1052 449
Fragments arranged by size:
1345 1052 751 467 449 417 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1443 2386 1443
Size: 943 4546
BtgI C'CryG_G
Cuts at: 107 146 311 693 774 107
Size: 39 165 382 81 4822
Fragments arranged by size:
4822 382 165 81 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1413 3439 3620 3783 1413
Size: 2026 181 163 3119
Fragments arranged by size:
3119 2026 181 163
BtsI GCAGTG_nn'
Cuts at: 742 927 1224 2446 2732 742
Size: 185 297 1222 286 3499
Fragments arranged by size:
3499 1222 297 286 185
DraI TTT'AAA
Cuts at: 2488 2488
Size: 5489
DrdI GACnn_nn'nnGTC
Cuts at: 3195 4539 3195
Size: 1344 4145
EaeI y'GGCC_r
Cuts at: 143 149 158 237 599 608 887 1463 1560
Size: 6 9 79 362 9 279 576 97
Cuts at: 1560 1784 2359 3074 3248 3639 3666 5011 143
Size: 224 575 715 174 391 27 1345 621
Fragments arranged by size:
1345 715 621 576 575 391 362 279
224 174 97 79 27 9 9 6
EagI C'GGCC_G
Cuts at: 608 3074 608
Size: 2466 3023
EarI CTCTTCn'nnn_
Cuts at: 2153 3512 3722 2153
Size: 1359 210 3920
Fragments arranged by size:
3920 1359 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1591 3697 4439 4585 5299 1591
Size: 2106 742 146 714 1781
Fragments arranged by size:
2106 1781 742 714 146
EcoICRI GAG'CTC
Cuts at: 1406 1479 2146 2501 1406
Size: 73 667 355 4394
Fragments arranged by size:
4394 667 355 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 694 865 1405 2180 2362 3314 3746 4099
Size: 241 171 540 775 182 952 432 353
Cuts at: 4099 4761 453
Size: 662 1181
Fragments arranged by size:
1181 952 775 662 540 432 353 241
182 171
EcoNI CCTnn'n_nnAGG
Cuts at: 1546 2248 1546
Size: 702 4787
EcoO109I rG'GnC_Cy
Cuts at: 316 376 377 422 554 555 1452 1453 1662
Size: 60 1 45 132 1 897 1 209
Cuts at: 1662 1757 1887 1959 2083 2278 2628 316
Size: 95 130 72 124 195 350 3177
Fragments arranged by size:
3177 897 350 209 195 132 130 124
95 72 60 45 1 1 1
EcoRI G'AATT_C
Cuts at: 2493 2493
Size: 5489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3508 3508
Size: 5489
FspI TGC'GCA
Cuts at: 1303 1426 3270 1303
Size: 123 1844 3522
Fragments arranged by size:
3522 1844 123
FspAI rTGC'GCAy
Cuts at: 1426 1426
Size: 5489
HaeII r_GCGC'y
Cuts at: 116 167 852 1049 1742 2336 2923 3171 4401
Size: 51 685 197 693 594 587 248 1230
Cuts at: 4401 5172 116
Size: 771 433
Fragments arranged by size:
1230 771 693 685 594 587 433 248
197 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 474 478 506 510 1663 1695 1879 1911 3331
Size: 4 28 4 1153 32 184 32 1420
Cuts at: 3331 3361 3363 3393 474
Size: 30 2 30 2570
Fragments arranged by size:
2570 1420 1153 184 32 32 30 30
28 4 4 2
HincII GTy'rAC
Cuts at: 2528 2528
Size: 5489
KasI G'GCGC_C
Cuts at: 112 163 1738 2332 2919 3167 112
Size: 51 1575 594 587 248 2434
Fragments arranged by size:
2434 1575 594 587 248 51
KpnI G_GTAC'C
Cuts at: 2509 2509
Size: 5489
MluI A'CGCG_T
Cuts at: 1394 4922 1394
Size: 3528 1961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1433 1649 4248 4432 1415
Size: 18 216 2599 184 2472
Fragments arranged by size:
2599 2472 216 184 18
MscI TGG'CCA
Cuts at: 151 1465 2361 3250 151
Size: 1314 896 889 2390
Fragments arranged by size:
2390 1314 896 889
MslI CAynn'nnrTG
Cuts at: 470 541 1270 2135 2216 3478 3605 470
Size: 71 729 865 81 1262 127 2354
Fragments arranged by size:
2354 1262 865 729 127 81 71
MspA1I CmG'CkG
Cuts at: 266 384 431 441 632 643 695 776 2269
Size: 118 47 10 191 11 52 81 1493
Cuts at: 2269 2600 2914 3274 4058 4303 266
Size: 331 314 360 784 245 1452
Fragments arranged by size:
1493 1452 784 360 331 314 245 191
118 81 52 47 11 10
NaeI GCC'GGC
Cuts at: 162 436 608 887 3670 162
Size: 274 172 279 2783 1981
Fragments arranged by size:
2783 1981 279 274 172
NarI GG'CG_CC
Cuts at: 113 164 1739 2333 2920 3168 113
Size: 51 1575 594 587 248 2434
Fragments arranged by size:
2434 1575 594 587 248 51
NcoI C'CATG_G
Cuts at: 107 311 107
Size: 204 5285
NgoMIV G'CCGG_C
Cuts at: 160 434 606 885 3668 160
Size: 274 172 279 2783 1981
Fragments arranged by size:
2783 1981 279 274 172
NspI r_CATG'y
Cuts at: 2237 2542 3042 4645 2237
Size: 305 500 1603 3081
Fragments arranged by size:
3081 1603 500 305
PasI CC'CwG_GG
Cuts at: 1530 1530
Size: 5489
PciI A'CATG_T
Cuts at: 4641 4641
Size: 5489
PflMI CCAn_nnn'nTGG
Cuts at: 326 643 668 2176 326
Size: 317 25 1508 3639
Fragments arranged by size:
3639 1508 317 25
PmeI GTTT'AAAC
Cuts at: 2488 2488
Size: 5489
PmlI CAC'GTG
Cuts at: 978 978
Size: 5489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 5457
PpuMI rG'GwC_Cy
Cuts at: 1662 1887 1959 2083 2278 1662
Size: 225 72 124 195 4873
Fragments arranged by size:
4873 225 195 124 72
PspOMI G'GGCC_C
Cuts at: 225 316 376 554 1002 1452 1517 1756 225
Size: 91 60 178 448 450 65 239 3958
Fragments arranged by size:
3958 450 448 239 178 91 65 60
PspXI vC'TCGA_Gb
Cuts at: 1480 1480
Size: 5489
PstI C_TGCA'G
Cuts at: 392 875 2536 3221 392
Size: 483 1661 685 2660
Fragments arranged by size:
2660 1661 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5489
PvuII CAG'CTG
Cuts at: 431 2269 2914 3274 431
Size: 1838 645 360 2646
Fragments arranged by size:
2646 1838 645 360
RsrII CG'GwC_CG
Cuts at: 771 3684 771
Size: 2913 2576
SacI G_AGCT'C
Cuts at: 1408 1481 2148 2503 1408
Size: 73 667 355 4394
Fragments arranged by size:
4394 667 355 73
SacII CC_GC'GG
Cuts at: 696 777 696
Size: 81 5408
SalI G'TCGA_C
Cuts at: 2526 2526
Size: 5489
SapI GCTCTTCn'nnn_
Cuts at: 2153 3512 3722 2153
Size: 1359 210 3920
Fragments arranged by size:
3920 1359 210
SbfI CC_TGCA'GG
Cuts at: 875 2536 875
Size: 1661 3828
ScaI AGT'ACT
Cuts at: 1930 2348 5006 1930
Size: 418 2658 2413
Fragments arranged by size:
2658 2413 418
SexAI A'CCwGG_T
Cuts at: 1673 1889 1907 2069 1673
Size: 216 18 162 5093
Fragments arranged by size:
5093 216 162 18
SfcI C'TryA_G
Cuts at: 33 388 426 871 2532 3217 4185 4376 33
Size: 355 38 445 1661 685 968 191 1146
Fragments arranged by size:
1661 1146 968 685 445 355 191 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 157 607 157
Size: 450 5039
SfoI GGC'GCC
Cuts at: 114 165 1740 2334 2921 3169 114
Size: 51 1575 594 587 248 2434
Fragments arranged by size:
2434 1575 594 587 248 51
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5489
SmaI CCC'GGG
Cuts at: 224 300 1829 1918 2029 2355 2511 224
Size: 76 1529 89 111 326 156 3202
Fragments arranged by size:
3202 1529 326 156 111 89 76
SmlI C'TyrA_G
Cuts at: 970 1033 1363 1480 2644 3559 4273 4535 970
Size: 63 330 117 1164 915 714 262 1924
Fragments arranged by size:
1924 1164 915 714 330 262 117 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5489
SphI G_CATG'C
Cuts at: 2237 2542 3042 2237
Size: 305 500 4684
Fragments arranged by size:
4684 500 305
StuI AGG'CCT
Cuts at: 1322 1322
Size: 5489
StyI C'CwwG_G
Cuts at: 107 179 311 1542 1787 2623 2977 107
Size: 72 132 1231 245 836 354 2619
Fragments arranged by size:
2619 1231 836 354 245 132 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2002 2449 3072 3205 3664 3873 2002
Size: 447 623 133 459 209 3618
Fragments arranged by size:
3618 623 459 447 209 133
TatI w'GTAC_w
Cuts at: 938 1115 1928 2208 2346 5004 938
Size: 177 813 280 138 2658 1423
Fragments arranged by size:
2658 1423 813 280 177 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 400 432 1333 1365 5006 5038 400
Size: 32 901 32 3641 32 851
Fragments arranged by size:
3641 901 851 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2288 3286 2288
Size: 998 4491
XbaI T'CTAG_A
Cuts at: 2520 2520
Size: 5489
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1320 1416 2984 1320
Size: 96 1568 3825
Fragments arranged by size:
3825 1568 96
XhoI C'TCGA_G
Cuts at: 1480 1480
Size: 5489
XmaI C'CCGG_G
Cuts at: 222 298 1827 1916 2027 2353 2509 222
Size: 76 1529 89 111 326 156 3202
Fragments arranged by size:
3202 1529 326 156 111 89 76
ZraI GAC'GTC
Cuts at: 2304 2304
Size: 5489
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmBI BspEI BssHII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PsrI
SanDI SgrAI SnaBI SrfI SspI SwaI XmnI