Restriction map for
pF1KB4458
(Circular) (Six-base) MAPSORT of: pF1KB4458.seq Check: 9225 from: 1 to: 6743
pF1KB4458 6743 bp
With 182 enzymes: SgfI *
March 2, 2009 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 904 985 1825 904
Size: 81 840 5822
Fragments arranged by size:
5822 840 81
AccI GT'mk_AC
Cuts at: 2936 3781 2936
Size: 845 5898
Acc65I G'GTAC_C
Cuts at: 3759 3759
Size: 6743
AclI AA'CG_TT
Cuts at: 6583 6583
Size: 6743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 3162 4568 5000 5747 6015 523
Size: 2639 1406 432 747 268 1251
Fragments arranged by size:
2639 1406 1251 747 432 268
AfeI AGC'GCT
Cuts at: 137 3037 137
Size: 2900 3843
AflIII A'CryG_T
Cuts at: 632 2426 4095 5199 6176 632
Size: 1794 1669 1104 977 1199
Fragments arranged by size:
1794 1669 1199 1104 977
AgeI A'CCGG_T
Cuts at: 5131 5131
Size: 6743
AhdI GACnn_n'nnGTC
Cuts at: 1559 1559
Size: 6743
AleI CACnn'nnGTG
Cuts at: 979 2789 3502 979
Size: 1810 713 4220
Fragments arranged by size:
4220 1810 713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1429 1461 1429
Size: 32 6711
AlwNI CAG_nnn'CTG
Cuts at: 735 797 1281 2547 3376 3450 4048 5615 735
Size: 62 484 1266 829 74 598 1567 1863
Fragments arranged by size:
1863 1567 1266 829 598 484 74 62
ApaI G_GGCC'C
Cuts at: 1660 2067 3582 1660
Size: 407 1515 4821
Fragments arranged by size:
4821 1515 407
ApaLI G'TGCA_C
Cuts at: 2608 5513 2608
Size: 2905 3838
ApoI r'AATT_y
Cuts at: 297 401 450 522 865 1138 1509 2239 2324
Size: 104 49 72 343 273 371 730 85
Cuts at: 2324 3007 3724 3747 6096 6352 297
Size: 683 717 23 2349 256 688
Fragments arranged by size:
2349 730 717 688 683 371 343 273
256 104 85 72 49 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 6743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6743
AvaI C'yCGr_G
Cuts at: 836 949 1642 3023 3514 3763 836
Size: 113 693 1381 491 249 3816
Fragments arranged by size:
3816 1381 693 491 249 113
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1827 1860 1827
Size: 33 6710
BamHI G'GATC_C
Cuts at: 1269 2300 2839 3768 1269
Size: 1031 539 929 4244
Fragments arranged by size:
4244 1031 929 539
BanI G'GyrC_C
Cuts at: 544 970 2117 3331 3759 4173 4421 4456 544
Size: 426 1147 1214 428 414 248 35 2831
Fragments arranged by size:
2831 1214 1147 428 426 414 248 35
BanII G_rGCy'C
Cuts at: 180 1660 1667 2067 2266 3217 3582 3676 3757
Size: 1480 7 400 199 951 365 94 81
Cuts at: 3757 4787 180
Size: 1030 2136
Fragments arranged by size:
2136 1480 1030 951 400 365 199 94
81 7
BbeI G_GCGC'C
Cuts at: 548 2121 3335 4177 4425 548
Size: 1573 1214 842 248 2866
Fragments arranged by size:
2866 1573 1214 842 248
BbsI GAAGACnn'nnnn_
Cuts at: 1073 3128 3299 1073
Size: 2055 171 4517
Fragments arranged by size:
4517 2055 171
BbvCI CC'TCA_GC
Cuts at: 2909 2909
Size: 6743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3550 3584 90
Size: 34 3426 34 3249
Fragments arranged by size:
3426 3249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1855 4641 5402 6743 1855
Size: 2786 761 1341 1855
Fragments arranged by size:
2786 1855 1341 761
BclI T'GATC_A
Cuts at: 2942 2999 4263 2942
Size: 57 1264 5422
Fragments arranged by size:
5422 1264 57
BfrBI ATG'CAT
Cuts at: 3069 3069
Size: 6743
BglI GCCn_nnn'nGGC
Cuts at: 1042 2441 3577 1042
Size: 1399 1136 4208
Fragments arranged by size:
4208 1399 1136
BglII A'GATC_T
Cuts at: 2873 4258 6295 2873
Size: 1385 2037 3321
Fragments arranged by size:
3321 2037 1385
BlpI GC'TnA_GC
Cuts at: 3855 3855
Size: 6743
Bme1580I G_kGCm'C
Cuts at: 235 756 1660 1857 2067 2612 3108 3582 4368
Size: 521 904 197 210 545 496 474 786
Cuts at: 4368 4461 5517 235
Size: 93 1056 1461
Fragments arranged by size:
1461 1056 904 786 545 521 496 474
210 197 93
BmgBI CAC'GTC
Cuts at: 3135 3194 3135
Size: 59 6684
BmrI ACTGGGnnnn_n'
Cuts at: 785 2060 2183 2535 3364 3585 3955 4036 4371
Size: 1275 123 352 829 221 370 81 335
Cuts at: 4371 6515 785
Size: 2144 1013
Fragments arranged by size:
2144 1275 1013 829 370 352 335 221
123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 194 2895 2920 5110 194
Size: 2701 25 2190 1827
Fragments arranged by size:
2701 2190 1827 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 244 1753 2820 2908 202
Size: 42 1509 1067 88 4037
Fragments arranged by size:
4037 1509 1067 88 42
Bpu10I CC'TnA_GC
Cuts at: 462 2909 3211 462
Size: 2447 302 3994
Fragments arranged by size:
3994 2447 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2238 3919 4798 5290 5588 1450
Size: 788 1681 879 492 298 2605
Fragments arranged by size:
2605 1681 879 788 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3020 3464 6447 3020
Size: 444 2983 3316
Fragments arranged by size:
3316 2983 444
BsaAI yAC'GTr
Cuts at: 884 929 2400 2427 4096 884
Size: 45 1471 27 1669 3531
Fragments arranged by size:
3531 1669 1471 45 27
BsaBI GATnn'nnATC
Cuts at: 6 4257 6294 6
Size: 4251 2037 455
Fragments arranged by size:
4251 2037 455
BsaHI Gr'CG_yC
Cuts at: 545 1073 1943 2118 3332 4174 4422 5124 6620
Size: 528 870 175 1214 842 248 702 1496
Cuts at: 6620 545
Size: 668
Fragments arranged by size:
1496 1214 870 842 702 668 528 248
175
BsaWI w'CCGG_w
Cuts at: 496 1558 2266 3932 4453 5131 5405 5552 5963
Size: 1062 708 1666 521 678 274 147 411
Cuts at: 5963 496
Size: 1276
Fragments arranged by size:
1666 1276 1062 708 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1138 1168 1180 1210 1242 1272 2717 2747 1138
Size: 30 12 30 32 30 1445 30 5134
Fragments arranged by size:
5134 1445 32 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 733 1250 1253 2059 2868 733
Size: 517 3 806 809 4608
Fragments arranged by size:
4608 809 806 517 3
BseYI C'CCAG_C
Cuts at: 704 755 1652 2259 2439 2691 3032 3463 4168
Size: 51 897 607 180 252 341 431 705
Cuts at: 4168 4926 5503 5999 704
Size: 758 577 496 1448
Fragments arranged by size:
1448 897 758 705 607 577 496 431
341 252 180 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 731 810 1176 1803 1962 2584 3442 337
Size: 394 79 366 627 159 622 858 3638
Fragments arranged by size:
3638 858 627 622 394 366 159 79
BsiEI CG_ry'CG
Cuts at: 104 128 4285 4331 5539 104
Size: 24 4157 46 1208 1308
Fragments arranged by size:
4157 1308 1208 46 24
BsiHKAI G_wGCw'C
Cuts at: 766 1639 2612 3217 3757 4535 4725 5517 766
Size: 873 973 605 540 778 190 792 1992
Fragments arranged by size:
1992 973 873 792 778 605 540 190
BsmI GAATG_Cn'
Cuts at: 1714 3071 1714
Size: 1357 5386
BsmBI CGTCTCn'nnnn_
Cuts at: 866 1683 866
Size: 817 5926
Bsp1286I G_dGCh'C
Cuts at: 180 235 756 766 1639 1660 1667 1857 2067
Size: 55 521 10 873 21 7 190 210
Cuts at: 2067 2266 2612 3108 3217 3582 3676 3757 4368
Size: 199 346 496 109 365 94 81 611
Cuts at: 4368 4461 4535 4725 4787 5517 180
Size: 93 74 190 62 730 1406
Fragments arranged by size:
1406 873 730 611 521 496 365 346
210 199 190 190 109 94 93 81
74 62 55 21 10 7
BspEI T'CCGG_A
Cuts at: 5963 5963
Size: 6743
BspHI T'CATG_A
Cuts at: 5919 5919
Size: 6743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 425 904 985 1825 1897 2555 3793 4007 4309
Size: 479 81 840 72 658 1238 214 302
Cuts at: 4309 4690 425
Size: 381 2478
Fragments arranged by size:
2478 1238 840 658 479 381 302 214
81 72
BsrBI CCG'CTC
Cuts at: 50 5035 5089 6573 6738 50
Size: 4985 54 1484 165 55
Fragments arranged by size:
4985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 377 4655 377
Size: 4278 2465
BsrFI r'CCGG_y
Cuts at: 1224 1945 4741 4922 5131 1224
Size: 721 2796 181 209 2836
Fragments arranged by size:
2836 2796 721 209 181
BssSI C'ACGA_G
Cuts at: 207 1023 5014 5372 207
Size: 816 3991 358 1578
Fragments arranged by size:
3991 1578 816 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1709 1778 2484 4120 1709
Size: 69 706 1636 4332
Fragments arranged by size:
4332 1636 706 69
BstBI TT'CG_AA
Cuts at: 5104 5104
Size: 6743
BstEII G'GTnAC_C
Cuts at: 2811 2811
Size: 6743
BstXI CCAn_nnnn'nTGG
Cuts at: 2055 2691 2055
Size: 636 6107
BstYI r'GATC_y
Cuts at: 1 1269 2033 2222 2300 2839 2873 3768 4235
Size: 1268 764 189 78 539 34 895 467
Cuts at: 4235 4258 4593 4839 5851 5937 5949 6295 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1268 1012 895 764 539 467 449 346
335 246 189 86 78 34 23 12
Bsu36I CC'TnA_GG
Cuts at: 1799 3700 1799
Size: 1901 4842
BtgI C'CryG_G
Cuts at: 777 3245 777
Size: 2468 4275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2028 4693 4874 5037 2028
Size: 2665 181 163 3734
Fragments arranged by size:
3734 2665 181 163
BtsI GCAGTG_nn'
Cuts at: 621 766 1875 3497 3509 3726 3986 621
Size: 145 1109 1622 12 217 260 3378
Fragments arranged by size:
3378 1622 1109 260 217 145 12
ClaI AT'CG_AT
Cuts at: 2930 2930
Size: 6743
DraI TTT'AAA
Cuts at: 1143 3742 1143
Size: 2599 4144
DraIII CAC_nnn'GTG
Cuts at: 2191 2191
Size: 6743
DrdI GACnn_nn'nnGTC
Cuts at: 853 4449 5307 853
Size: 3596 858 2289
Fragments arranged by size:
3596 2289 858
EaeI y'GGCC_r
Cuts at: 125 1947 2405 2681 4328 4502 4893 4920 6265
Size: 1822 458 276 1647 174 391 27 1345
Cuts at: 6265 125
Size: 603
Fragments arranged by size:
1822 1647 1345 603 458 391 276 174
27
EagI C'GGCC_G
Cuts at: 125 4328 125
Size: 4203 2540
EarI CTCTTCn'nnn_
Cuts at: 2110 2696 4766 4976 2110
Size: 586 2070 210 3877
Fragments arranged by size:
3877 2070 586 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4951 5261 5407 6553 4951
Size: 310 146 1146 5141
Fragments arranged by size:
5141 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3215 3755 3215
Size: 540 6203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 244 523 1753 2820 2908 3162 4568 5000
Size: 42 279 1230 1067 88 254 1406 432
Cuts at: 5000 5747 6015 202
Size: 747 268 930
Fragments arranged by size:
1406 1230 1067 930 747 432 279 268
254 88 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1732 1798 1732
Size: 66 6677
EcoO109I rG'GnC_Cy
Cuts at: 832 1099 1553 1656 2063 2064 3486 3882 832
Size: 267 454 103 407 1 1422 396 3693
Fragments arranged by size:
3693 1422 454 407 396 267 103 1
EcoRI G'AATT_C
Cuts at: 401 2239 3007 3747 401
Size: 1838 768 740 3397
Fragments arranged by size:
3397 1838 768 740
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4762 4762
Size: 6743
FspI TGC'GCA
Cuts at: 4524 4524
Size: 6743
HaeII r_GCGC'y
Cuts at: 139 148 548 774 2121 3039 3335 4177 4425
Size: 9 400 226 1347 918 296 842 248
Cuts at: 4425 5447 6426 139
Size: 1022 979 456
Fragments arranged by size:
1347 1022 979 918 842 456 400 296
248 226 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1006 1038 1138 1170 1545 1577 1663 1695 2285
Size: 32 100 32 375 32 86 32 590
Cuts at: 2285 2317 4585 4615 4617 4647 1006
Size: 32 2268 30 2 30 3102
Fragments arranged by size:
3102 2268 590 375 100 86 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3549 3782 3549
Size: 233 6510
KasI G'GCGC_C
Cuts at: 544 2117 3331 4173 4421 544
Size: 1573 1214 842 248 2866
Fragments arranged by size:
2866 1573 1214 842 248
KpnI G_GTAC'C
Cuts at: 3763 3763
Size: 6743
MluI A'CGCG_T
Cuts at: 6176 6176
Size: 6743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2041 3116 5414 5598 2041
Size: 1075 2298 184 3186
Fragments arranged by size:
3186 2298 1075 184
MscI TGG'CCA
Cuts at: 2407 2683 4504 2407
Size: 276 1821 4646
Fragments arranged by size:
4646 1821 276
MslI CAynn'nnrTG
Cuts at: 637 979 2053 2789 2919 2944 3502 4732 4859
Size: 342 1074 736 130 25 558 1230 127
Cuts at: 4859 637
Size: 2521
Fragments arranged by size:
2521 1230 1074 736 558 342 130 127
25
MspA1I CmG'CkG
Cuts at: 665 1601 3412 3682 3854 4168 4528 5541 5786
Size: 936 1811 270 172 314 360 1013 245
Cuts at: 5786 665
Size: 1622
Fragments arranged by size:
1811 1622 1013 936 360 314 270 245
172
NaeI GCC'GGC
Cuts at: 1947 4924 1947
Size: 2977 3766
NarI GG'CG_CC
Cuts at: 545 2118 3332 4174 4422 545
Size: 1573 1214 842 248 2866
Fragments arranged by size:
2866 1573 1214 842 248
NdeI CA'TA_TG
Cuts at: 2850 2920 2850
Size: 70 6673
NgoMIV G'CCGG_C
Cuts at: 1945 4922 1945
Size: 2977 3766
NsiI A_TGCA'T
Cuts at: 3071 3071
Size: 6743
NspI r_CATG'y
Cuts at: 636 2659 3796 4296 5203 636
Size: 2023 1137 500 907 2176
Fragments arranged by size:
2176 2023 1137 907 500
PasI CC'CwG_GG
Cuts at: 2091 2091
Size: 6743
PciI A'CATG_T
Cuts at: 632 5199 632
Size: 4567 2176
PflMI CCAn_nnn'nTGG
Cuts at: 360 2023 360
Size: 1663 5080
PfoI T'CCnGG_A
Cuts at: 118 353 2501 2513 2885 118
Size: 235 2148 12 372 3976
Fragments arranged by size:
3976 2148 372 235 12
PmeI GTTT'AAAC
Cuts at: 3742 3742
Size: 6743
PmlI CAC'GTG
Cuts at: 884 2427 884
Size: 1543 5200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5882 5914 5882
Size: 32 6711
PpuMI rG'GwC_Cy
Cuts at: 1553 3486 1553
Size: 1933 4810
PsiI TTA'TAA
Cuts at: 1579 1579
Size: 6743
PspOMI G'GGCC_C
Cuts at: 1656 2063 3578 1656
Size: 407 1515 4821
Fragments arranged by size:
4821 1515 407
PstI C_TGCA'G
Cuts at: 422 822 2159 3504 3790 4475 422
Size: 400 1337 1345 286 685 2690
Fragments arranged by size:
2690 1345 1337 685 400 286
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6743
PvuII CAG'CTG
Cuts at: 665 1601 3682 4168 4528 665
Size: 936 2081 486 360 2880
Fragments arranged by size:
2880 2081 936 486 360
RsrII CG'GwC_CG
Cuts at: 4938 4938
Size: 6743
SacI G_AGCT'C
Cuts at: 3217 3757 3217
Size: 540 6203
SalI G'TCGA_C
Cuts at: 3780 3780
Size: 6743
SapI GCTCTTCn'nnn_
Cuts at: 4766 4976 4766
Size: 210 6533
SbfI CC_TGCA'GG
Cuts at: 3790 3790
Size: 6743
ScaI AGT'ACT
Cuts at: 6260 6260
Size: 6743
SfcI C'TryA_G
Cuts at: 33 418 818 2155 3083 3500 3786 4471 5464
Size: 385 400 1337 928 417 286 685 993
Cuts at: 5464 5655 33
Size: 191 1121
Fragments arranged by size:
1337 1121 993 928 685 417 400 385
286 191
SfoI GGC'GCC
Cuts at: 546 2119 3333 4175 4423 546
Size: 1573 1214 842 248 2866
Fragments arranged by size:
2866 1573 1214 842 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6743
SmaI CCC'GGG
Cuts at: 838 3765 838
Size: 2927 3816
SmlI C'TyrA_G
Cuts at: 1465 2217 3898 4813 5305 5567 1465
Size: 752 1681 915 492 262 2641
Fragments arranged by size:
2641 1681 915 752 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6743
SphI G_CATG'C
Cuts at: 3796 4296 3796
Size: 500 6243
SspI AAT'ATT
Cuts at: 1173 2532 1173
Size: 1359 5384
StyI C'CwwG_G
Cuts at: 201 291 1448 1667 1744 3641 3877 4231 201
Size: 90 1157 219 77 1897 236 354 2713
Fragments arranged by size:
2713 1897 1157 354 236 219 90 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1799 2202 2683 2931 4326 4459 4918 5127 1799
Size: 403 481 248 1395 133 459 209 3415
Fragments arranged by size:
3415 1395 481 459 403 248 209 133
TatI w'GTAC_w
Cuts at: 440 6258 440
Size: 5818 925
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1242 1274 2717 2749 6260 6292 1242
Size: 32 1443 32 3511 32 1693
Fragments arranged by size:
3511 1693 1443 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 607 1072 1675 2509 4540 607
Size: 465 603 834 2031 2810
Fragments arranged by size:
2810 2031 834 603 465
XbaI T'CTAG_A
Cuts at: 3774 3774
Size: 6743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 357 361 933 2055 2634 4238 357
Size: 4 572 1122 579 1604 2862
Fragments arranged by size:
2862 1604 1122 579 572 4
XmaI C'CCGG_G
Cuts at: 836 3763 836
Size: 2927 3816
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AscI AvrII BmtI BsiWI BsrGI
BssHII BstZ17I CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NheI NotI NruI PacI PshAI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI