Restriction map for
pF1KB4461
(Circular) (Six-base) MAPSORT of: pF1KB4461.seq Check: 8702 from: 1 to: 4835
pF1KB4461 4835 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 135 135
Size: 4835
AccI GT'mk_AC
Cuts at: 1873 1873
Size: 4835
Acc65I G'GTAC_C
Cuts at: 1851 1851
Size: 4835
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1529 2660 3092 3445 4107 1529
Size: 1131 432 353 662 2257
Fragments arranged by size:
2257 1131 662 432 353
AfeI AGC'GCT
Cuts at: 735 735
Size: 4835
AflIII A'CryG_T
Cuts at: 479 2187 3987 4268 479
Size: 1708 1800 281 1046
Fragments arranged by size:
1800 1708 1046 281
AlwNI CAG_nnn'CTG
Cuts at: 2140 3578 2140
Size: 1438 3397
ApaLI G'TGCA_C
Cuts at: 3673 3673
Size: 4835
ApoI r'AATT_y
Cuts at: 1839 4188 4444 1839
Size: 2349 256 2230
Fragments arranged by size:
2349 2230 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 1855 1855
Size: 4835
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 124 200 771 1186 1851 2265 2513 2548 124
Size: 76 571 415 665 414 248 35 2411
Fragments arranged by size:
2411 665 571 415 414 248 76 35
BanII G_rGCy'C
Cuts at: 1849 2879 1849
Size: 1030 3805
BbeI G_GCGC'C
Cuts at: 2269 2517 2269
Size: 248 4587
BciVI GTATCCnnnnn_n'
Cuts at: 1441 1605 2733 3789 4835 1441
Size: 164 1128 1056 1046 1441
Fragments arranged by size:
1441 1128 1056 1046 164
BclI T'GATC_A
Cuts at: 950 956 1067 2355 950
Size: 6 111 1288 3430
Fragments arranged by size:
3430 1288 111 6
BglI GCCn_nnn'nGGC
Cuts at: 455 1408 455
Size: 953 3882
BglII A'GATC_T
Cuts at: 2350 4387 2350
Size: 2037 2798
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 460 1189 2460 2553 3677 460
Size: 729 1271 93 1124 1618
Fragments arranged by size:
1618 1271 1124 729 93
BmrI ACTGGGnnnn_n'
Cuts at: 517 1054 1430 2047 2128 2463 4607 517
Size: 537 376 617 81 335 2144 745
Fragments arranged by size:
2144 745 617 537 376 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3202 3202
Size: 4835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 829 387
Size: 442 4393
Bpu10I CC'TnA_GC
Cuts at: 437 437
Size: 4835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 2011 2890 3604 3902 538
Size: 1473 879 714 298 1471
Fragments arranged by size:
1473 1471 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4539 4539
Size: 4835
BsaAI yAC'GTr
Cuts at: 2188 2188
Size: 4835
BsaBI GATnn'nnATC
Cuts at: 6 793 955 2349 4386 6
Size: 787 162 1394 2037 455
Fragments arranged by size:
2037 1394 787 455 162
BsaHI Gr'CG_yC
Cuts at: 2266 2514 3216 4712 2266
Size: 248 702 1496 2389
Fragments arranged by size:
2389 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2024 2545 3223 3634 3781 4055 2024
Size: 521 678 411 147 274 2804
Fragments arranged by size:
2804 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1126 1156 1126
Size: 30 4805
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 730 793 1450 730
Size: 63 657 4115
Fragments arranged by size:
4115 657 63
BseYI C'CCAG_C
Cuts at: 228 564 1576 1625 2260 3018 3683 4091 228
Size: 336 1012 49 635 758 665 408 972
Fragments arranged by size:
1012 972 758 665 635 408 336 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1712 1712
Size: 4835
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3653 104
Size: 2273 46 1230 1286
Fragments arranged by size:
2273 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1849 2627 2817 3677 1849
Size: 778 190 860 3007
Fragments arranged by size:
3007 860 778 190
BsmI GAATG_Cn'
Cuts at: 164 1759 164
Size: 1595 3240
Bsp1286I G_dGCh'C
Cuts at: 460 1189 1849 2460 2553 2627 2817 2879 3677
Size: 729 660 611 93 74 190 62 798
Cuts at: 3677 460
Size: 1618
Fragments arranged by size:
1618 798 729 660 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 362 953 3267 362
Size: 591 2314 1930
Fragments arranged by size:
2314 1930 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 135 1885 2099 2401 2782 135
Size: 1750 214 302 381 2188
Fragments arranged by size:
2188 1750 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3127 3181 4665 4830 50
Size: 3077 54 1484 165 55
Fragments arranged by size:
3077 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 854 1007 2747 494
Size: 360 153 1740 2582
Fragments arranged by size:
2582 1740 360 153
BsrFI r'CCGG_y
Cuts at: 197 2833 3014 197
Size: 2636 181 2018
Fragments arranged by size:
2636 2018 181
BsrGI T'GTAC_A
Cuts at: 1052 1052
Size: 4835
BssSI C'ACGA_G
Cuts at: 459 3106 3814 459
Size: 2647 708 1480
Fragments arranged by size:
2647 1480 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1635 2212 1635
Size: 577 4258
BstBI TT'CG_AA
Cuts at: 624 3196 624
Size: 2572 2263
BstEII G'GTnAC_C
Cuts at: 1449 1449
Size: 4835
BstXI CCAn_nnnn'nTGG
Cuts at: 147 903 1299 1354 147
Size: 756 396 55 3628
Fragments arranged by size:
3628 756 396 55
BstYI r'GATC_y
Cuts at: 1 1860 2327 2350 2685 2931 3237 3249 3335
Size: 1859 467 23 335 246 306 12 86
Cuts at: 3335 4387 1
Size: 1052 449
Fragments arranged by size:
1859 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 467 1086 1475 107
Size: 360 619 389 3467
Fragments arranged by size:
3467 619 389 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 852 2785 2966 3129 852
Size: 1933 181 163 2558
Fragments arranged by size:
2558 1933 181 163
BtsI GCAGTG_nn'
Cuts at: 287 1175 1803 2078 287
Size: 888 628 275 3044
Fragments arranged by size:
3044 888 628 275
DraI TTT'AAA
Cuts at: 1834 1834
Size: 4835
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3885 2541
Size: 1344 3491
EaeI y'GGCC_r
Cuts at: 447 1424 2420 2594 2985 3012 4357 447
Size: 977 996 174 391 27 1345 925
Fragments arranged by size:
1345 996 977 925 391 174 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 637 2858 3068 637
Size: 2221 210 2404
Fragments arranged by size:
2404 2221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 3785 3931 4645 3043
Size: 742 146 714 3233
Fragments arranged by size:
3233 742 714 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 829 1529 2660 3092 3445 4107 387
Size: 442 700 1131 432 353 662 1115
Fragments arranged by size:
1131 1115 700 662 442 432 353
EcoO109I rG'GnC_Cy
Cuts at: 568 804 1974 568
Size: 236 1170 3429
Fragments arranged by size:
3429 1170 236
EcoRI G'AATT_C
Cuts at: 1839 1839
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4835
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 737 2269 2517 3747 4518 737
Size: 1532 248 1230 771 1054
Fragments arranged by size:
1532 1230 1054 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 873 905 1572 1604 1647 1679 2677
Size: 32 264 32 667 32 43 32 998
Cuts at: 2677 2707 2709 2739 577
Size: 30 2 30 2673
Fragments arranged by size:
2673 998 667 264 43 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1874 1874
Size: 4835
HindIII A'AGCT_T
Cuts at: 1166 1166
Size: 4835
KasI G'GCGC_C
Cuts at: 2265 2513 2265
Size: 248 4587
KpnI G_GTAC'C
Cuts at: 1855 1855
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1263 3594 3778 1263
Size: 2331 184 2320
Fragments arranged by size:
2331 2320 184
MscI TGG'CCA
Cuts at: 2596 2596
Size: 4835
MslI CAynn'nnrTG
Cuts at: 323 367 871 958 1297 2824 2951 323
Size: 44 504 87 339 1527 127 2207
Fragments arranged by size:
2207 1527 504 339 127 87 44
MspA1I CmG'CkG
Cuts at: 564 1946 2260 2620 3404 3649 564
Size: 1382 314 360 784 245 1750
Fragments arranged by size:
1750 1382 784 360 314 245
NaeI GCC'GGC
Cuts at: 3016 3016
Size: 4835
NarI GG'CG_CC
Cuts at: 2266 2514 2266
Size: 248 4587
NcoI C'CATG_G
Cuts at: 107 467 107
Size: 360 4475
NgoMIV G'CCGG_C
Cuts at: 3014 3014
Size: 4835
NspI r_CATG'y
Cuts at: 483 1888 2388 3991 483
Size: 1405 500 1603 1327
Fragments arranged by size:
1603 1405 1327 500
PasI CC'CwG_GG
Cuts at: 349 975 1182 349
Size: 626 207 4002
Fragments arranged by size:
4002 626 207
PciI A'CATG_T
Cuts at: 479 3987 479
Size: 3508 1327
PfoI T'CCnGG_A
Cuts at: 611 1364 611
Size: 753 4082
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4803
PstI C_TGCA'G
Cuts at: 504 1798 1882 2567 504
Size: 1294 84 685 2772
Fragments arranged by size:
2772 1294 685 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 564 2260 2620 564
Size: 1696 360 2779
Fragments arranged by size:
2779 1696 360
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 2858 3068 2858
Size: 210 4625
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 506 4352 506
Size: 3846 989
SfcI C'TryA_G
Cuts at: 33 500 1794 1878 2563 3531 3722 33
Size: 467 1294 84 685 968 191 1146
Fragments arranged by size:
1294 1146 968 685 467 191 84
SfoI GGC'GCC
Cuts at: 2267 2515 2267
Size: 248 4587
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 1857 1857
Size: 4835
SmlI C'TyrA_G
Cuts at: 553 1990 2905 3619 3881 553
Size: 1437 915 714 262 1507
Fragments arranged by size:
1507 1437 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1888 2388 1888
Size: 500 4335
StuI AGG'CCT
Cuts at: 428 1076 428
Size: 648 4187
StyI C'CwwG_G
Cuts at: 107 467 1813 1969 2323 107
Size: 360 1346 156 354 2619
Fragments arranged by size:
2619 1346 360 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1549 2418 2551 3010 3219 1549
Size: 869 133 459 209 3165
Fragments arranged by size:
3165 869 459 209 133
TatI w'GTAC_w
Cuts at: 504 1052 4350 504
Size: 548 3298 989
Fragments arranged by size:
3298 989 548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1367 1399 4352 4384 1367
Size: 32 2953 32 1818
Fragments arranged by size:
2953 1818 32 32
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 903 1443 1605 2330 903
Size: 540 162 725 3408
Fragments arranged by size:
3408 725 540 162
XmaI C'CCGG_G
Cuts at: 1855 1855
Size: 4835
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsiWI BsmBI BspEI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI