Restriction map for
pF1KB4477
(Circular) (Six-base) MAPSORT of: pF1KB4477.seq Check: 2151 from: 1 to: 6560
pF1KB4477 6560 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 1430 1430
Size: 6560
AccI GT'mk_AC
Cuts at: 3598 3598
Size: 6560
Acc65I G'GTAC_C
Cuts at: 545 3576 545
Size: 3031 3529
AclI AA'CG_TT
Cuts at: 6400 6400
Size: 6560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 3439 3489 4385 4817 5170 5832 235
Size: 3204 50 896 432 353 662 963
Fragments arranged by size:
3204 963 896 662 432 353 50
AfeI AGC'GCT
Cuts at: 2299 2299
Size: 6560
AflIII A'CryG_T
Cuts at: 3356 3912 5712 5993 3356
Size: 556 1800 281 3923
Fragments arranged by size:
3923 1800 556 281
AhdI GACnn_n'nnGTC
Cuts at: 2385 3153 2385
Size: 768 5792
AleI CACnn'nnGTG
Cuts at: 1801 2432 1801
Size: 631 5929
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1078 1110 1078
Size: 32 6528
AlwNI CAG_nnn'CTG
Cuts at: 288 2881 3865 5303 288
Size: 2593 984 1438 1545
Fragments arranged by size:
2593 1545 1438 984
ApaI G_GGCC'C
Cuts at: 2770 2770
Size: 6560
ApaLI G'TGCA_C
Cuts at: 2744 5398 2744
Size: 2654 3906
ApoI r'AATT_y
Cuts at: 3564 5913 6169 3564
Size: 2349 256 3955
Fragments arranged by size:
3955 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6560
AvaI C'yCGr_G
Cuts at: 1082 1387 1485 2375 2492 2580 2646 2916 3188
Size: 305 98 890 117 88 66 270 272
Cuts at: 3188 3580 1082
Size: 392 4062
Fragments arranged by size:
4062 890 392 305 272 270 117 98
88 66
BamHI G'GATC_C
Cuts at: 3585 3585
Size: 6560
BanI G'GyrC_C
Cuts at: 545 2755 3238 3576 3990 4238 4273 545
Size: 2210 483 338 414 248 35 2832
Fragments arranged by size:
2832 2210 483 414 338 248 35
BanII G_rGCy'C
Cuts at: 791 1091 2181 2200 2587 2770 2917 3574 4604
Size: 300 1090 19 387 183 147 657 1030
Cuts at: 4604 791
Size: 2747
Fragments arranged by size:
2747 1090 1030 657 387 300 183 147
19
BbeI G_GCGC'C
Cuts at: 3242 3994 4242 3242
Size: 752 248 5560
Fragments arranged by size:
5560 752 248
BbsI GAAGACnn'nnnn_
Cuts at: 982 1996 2403 2547 3149 982
Size: 1014 407 144 602 4393
Fragments arranged by size:
4393 1014 602 407 144
BciVI GTATCCnnnnn_n'
Cuts at: 4458 5514 6560 4458
Size: 1056 1046 4458
Fragments arranged by size:
4458 1056 1046
BclI T'GATC_A
Cuts at: 1973 4080 1973
Size: 2107 4453
BglI GCCn_nnn'nGGC
Cuts at: 1550 2938 1550
Size: 1388 5172
BglII A'GATC_T
Cuts at: 1202 1251 3458 4075 6112 1202
Size: 49 2207 617 2037 1650
Fragments arranged by size:
2207 2037 1650 617 49
BlpI GC'TnA_GC
Cuts at: 586 2708 3672 586
Size: 2122 964 3474
Fragments arranged by size:
3474 2122 964
Bme1580I G_kGCm'C
Cuts at: 2100 2429 2748 2758 2770 4185 4278 5402 2100
Size: 329 319 10 12 1415 93 1124 3258
Fragments arranged by size:
3258 1415 1124 329 319 93 12 10
BmgBI CAC'GTC
Cuts at: 1803 1803
Size: 6560
BmrI ACTGGGnnnn_n'
Cuts at: 332 3772 3853 4188 6332 332
Size: 3440 81 335 2144 560
Fragments arranged by size:
3440 2144 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 555 1862 3043 4927 555
Size: 1307 1181 1884 2188
Fragments arranged by size:
2188 1884 1307 1181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 1246 1376 1752 2274 2571 2915 3203 3355
Size: 696 130 376 522 297 344 288 152
Cuts at: 3355 3454 550
Size: 99 3656
Fragments arranged by size:
3656 696 522 376 344 297 288 152
130 99
Bpu10I CC'TnA_GC
Cuts at: 1315 2199 2739 1315
Size: 884 540 5136
Fragments arranged by size:
5136 884 540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3736 4615 5329 5627 3736
Size: 879 714 298 4669
Fragments arranged by size:
4669 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2216 6264 2216
Size: 4048 2512
BsaAI yAC'GTr
Cuts at: 3913 3913
Size: 6560
BsaBI GATnn'nnATC
Cuts at: 6 4074 6111 6
Size: 4068 2037 455
Fragments arranged by size:
4068 2037 455
BsaHI Gr'CG_yC
Cuts at: 1427 1706 3239 3991 4239 4941 6437 1427
Size: 279 1533 752 248 702 1496 1550
Fragments arranged by size:
1550 1533 1496 752 702 279 248
BsaWI w'CCGG_w
Cuts at: 2207 3749 4270 4948 5359 5506 5780 2207
Size: 1542 521 678 411 147 274 2987
Fragments arranged by size:
2987 1542 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 709 739 1078 1108 1396 1426 1600 1630 2344
Size: 30 339 30 288 30 174 30 714
Cuts at: 2344 2374 2461 2491 2788 2818 709
Size: 30 87 30 297 30 4451
Fragments arranged by size:
4451 714 339 297 288 174 87 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 714 2284 2287 2791 2796 2805 3505 714
Size: 1570 3 504 5 9 700 3769
Fragments arranged by size:
3769 1570 700 504 9 5 3
BseYI C'CCAG_C
Cuts at: 491 906 1692 2130 2149 2161 3007 3096 3985
Size: 415 786 438 19 12 846 89 889
Cuts at: 3985 4743 5408 5816 491
Size: 758 665 408 1235
Fragments arranged by size:
1235 889 846 786 758 665 438 415
408 89 19 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1081 1367 3568 1062
Size: 19 286 2201 4054
Fragments arranged by size:
4054 2201 286 19
BsiEI CG_ry'CG
Cuts at: 104 2933 4102 4148 5378 104
Size: 2829 1169 46 1230 1286
Fragments arranged by size:
2829 1286 1230 1169 46
BsiHKAI G_wGCw'C
Cuts at: 1787 2748 3044 3574 4352 4542 5402 1787
Size: 961 296 530 778 190 860 2945
Fragments arranged by size:
2945 961 860 778 530 296 190
BsmBI CGTCTCn'nnnn_
Cuts at: 726 726
Size: 6560
Bsp1286I G_dGCh'C
Cuts at: 791 1091 1787 2100 2181 2200 2429 2587 2748
Size: 300 696 313 81 19 229 158 161
Cuts at: 2748 2758 2770 2917 3044 3574 4185 4278 4352
Size: 10 12 147 127 530 611 93 74
Cuts at: 4352 4542 4604 5402 791
Size: 190 62 798 1949
Fragments arranged by size:
1949 798 696 611 530 313 300 229
190 161 158 147 127 93 81 74
62 19 12 10
BspHI T'CATG_A
Cuts at: 1031 1115 4992 1031
Size: 84 3877 2599
Fragments arranged by size:
3877 2599 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1310 2616 2862 3610 3824 4126 4507 1310
Size: 1306 246 748 214 302 381 3363
Fragments arranged by size:
3363 1306 748 381 302 246 214
BsrBI CCG'CTC
Cuts at: 50 301 3225 4852 4906 6390 6555 50
Size: 251 2924 1627 54 1484 165 55
Fragments arranged by size:
2924 1627 1484 251 165 55 54
BsrDI GCAATG_nn'
Cuts at: 231 573 1873 2160 4472 231
Size: 342 1300 287 2312 2319
Fragments arranged by size:
2319 2312 1300 342 287
BsrFI r'CCGG_y
Cuts at: 1157 1610 2062 2263 2442 3340 4558 4739 1157
Size: 453 452 201 179 898 1218 181 2978
Fragments arranged by size:
2978 1218 898 453 452 201 181 179
BsrGI T'GTAC_A
Cuts at: 1187 1187
Size: 6560
BssSI C'ACGA_G
Cuts at: 2967 4831 5539 2967
Size: 1864 708 3988
Fragments arranged by size:
3988 1864 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2881 3937 2881
Size: 1056 5504
BstBI TT'CG_AA
Cuts at: 4921 4921
Size: 6560
BstEII G'GTnAC_C
Cuts at: 2487 2628 2487
Size: 141 6419
BstXI CCAn_nnnn'nTGG
Cuts at: 1002 2973 3255 1002
Size: 1971 282 4307
Fragments arranged by size:
4307 1971 282
BstYI r'GATC_y
Cuts at: 1 1202 1251 3458 3585 4052 4075 4410 4656
Size: 1201 49 2207 127 467 23 335 246
Cuts at: 4656 4962 4974 5060 6112 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2207 1201 1052 467 449 335 306 246
127 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 281 1144 2688 281
Size: 863 1544 4153
Fragments arranged by size:
4153 1544 863
BtgI C'CryG_G
Cuts at: 107 549 995 1175 1214 1710 2334 2390 2633
Size: 442 446 180 39 496 624 56 243
Cuts at: 2633 107
Size: 4034
Fragments arranged by size:
4034 624 496 446 442 243 180 56
39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4510 4691 4854 4510
Size: 181 163 6216
Fragments arranged by size:
6216 181 163
BtsI GCAGTG_nn'
Cuts at: 219 695 1600 2688 3335 3486 3803 219
Size: 476 905 1088 647 151 317 2976
Fragments arranged by size:
2976 1088 905 647 476 317 151
DraI TTT'AAA
Cuts at: 3559 3559
Size: 6560
DraIII CAC_nnn'GTG
Cuts at: 1293 2354 1293
Size: 1061 5499
DrdI GACnn_nn'nnGTC
Cuts at: 4266 5610 4266
Size: 1344 5216
EaeI y'GGCC_r
Cuts at: 256 383 483 639 749 779 968 1193 2040
Size: 127 100 156 110 30 189 225 847
Cuts at: 2040 2930 3047 3314 3491 4145 4319 4710 4737
Size: 890 117 267 177 654 174 391 27
Cuts at: 4737 6082 256
Size: 1345 734
Fragments arranged by size:
1345 890 847 734 654 391 267 225
189 177 174 156 127 117 110 100
30 27
EagI C'GGCC_G
Cuts at: 2930 4145 2930
Size: 1215 5345
EarI CTCTTCn'nnn_
Cuts at: 1001 2066 2452 3203 4583 4793 1001
Size: 1065 386 751 1380 210 2768
Fragments arranged by size:
2768 1380 1065 751 386 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2307 3057 4768 5510 5656 6370 2307
Size: 750 1711 742 146 714 2497
Fragments arranged by size:
2497 1711 750 742 714 146
EcoICRI GAG'CTC
Cuts at: 3572 3572
Size: 6560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 550 1246 1376 1752 2274 2571 2915 3203
Size: 315 696 130 376 522 297 344 288
Cuts at: 3203 3355 3439 3454 3489 4385 4817 5170 5832
Size: 152 84 15 35 896 432 353 662
Cuts at: 5832 235
Size: 963
Fragments arranged by size:
963 896 696 662 522 432 376 353
344 315 297 288 152 130 84 35
15
EcoNI CCTnn'n_nnAGG
Cuts at: 1501 1501
Size: 6560
EcoO109I rG'GnC_Cy
Cuts at: 495 1737 1763 1897 2166 2693 2766 2767 3024
Size: 1242 26 134 269 527 73 1 257
Cuts at: 3024 3083 3699 495
Size: 59 616 3356
Fragments arranged by size:
3356 1242 616 527 269 257 134 73
59 26 1
EcoRI G'AATT_C
Cuts at: 3564 3564
Size: 6560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1254 1974 3426 4579 1254
Size: 720 1452 1153 3235
Fragments arranged by size:
3235 1452 1153 720
FspI TGC'GCA
Cuts at: 4341 4341
Size: 6560
HaeII r_GCGC'y
Cuts at: 1545 1932 1970 2301 3242 3280 3994 4242 5472
Size: 387 38 331 941 38 714 248 1230
Cuts at: 5472 6243 1545
Size: 771 1862
Fragments arranged by size:
1862 1230 941 771 714 387 331 248
38 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1600 1632 2344 2371 2376 2403 2461 2493 3139
Size: 32 712 27 5 27 58 32 646
Cuts at: 3139 3171 3407 3439 4402 4432 4434 4464 1600
Size: 32 236 32 963 30 2 30 3696
Fragments arranged by size:
3696 963 712 646 236 58 32 32
32 32 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 522 3599 522
Size: 3077 3483
HindIII A'AGCT_T
Cuts at: 153 153
Size: 6560
KasI G'GCGC_C
Cuts at: 3238 3990 4238 3238
Size: 752 248 5560
Fragments arranged by size:
5560 752 248
KpnI G_GTAC'C
Cuts at: 549 3580 549
Size: 3031 3529
MluI A'CGCG_T
Cuts at: 5993 5993
Size: 6560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 286 1448 5319 5503 286
Size: 1162 3871 184 1343
Fragments arranged by size:
3871 1343 1162 184
MscI TGG'CCA
Cuts at: 385 485 641 1195 3316 4321 385
Size: 100 156 554 2121 1005 2624
Fragments arranged by size:
2624 2121 1005 554 156 100
MslI CAynn'nnrTG
Cuts at: 224 892 1000 1801 2432 4549 4676 224
Size: 668 108 801 631 2117 127 2108
Fragments arranged by size:
2117 2108 801 668 631 127 108
MspA1I CmG'CkG
Cuts at: 255 574 593 2125 2707 3236 3490 3671 3985
Size: 319 19 1532 582 529 254 181 314
Cuts at: 3985 4345 5129 5374 255
Size: 360 784 245 1441
Fragments arranged by size:
1532 1441 784 582 529 360 319 314
254 245 181 19
NaeI GCC'GGC
Cuts at: 2064 2444 3342 4741 2064
Size: 380 898 1399 3883
Fragments arranged by size:
3883 1399 898 380
NarI GG'CG_CC
Cuts at: 3239 3991 4239 3239
Size: 752 248 5560
Fragments arranged by size:
5560 752 248
NcoI C'CATG_G
Cuts at: 107 549 995 1214 2334 107
Size: 442 446 219 1120 4333
Fragments arranged by size:
4333 1120 446 442 219
NgoMIV G'CCGG_C
Cuts at: 2062 2442 3340 4739 2062
Size: 380 898 1399 3883
Fragments arranged by size:
3883 1399 898 380
NspI r_CATG'y
Cuts at: 1476 1589 3613 4113 5716 1476
Size: 113 2024 500 1603 2320
Fragments arranged by size:
2320 2024 1603 500 113
PciI A'CATG_T
Cuts at: 5712 5712
Size: 6560
PflMI CCAn_nnn'nTGG
Cuts at: 1376 1376
Size: 6560
PfoI T'CCnGG_A
Cuts at: 1133 1655 1768 2027 2183 3260 3446 1133
Size: 522 113 259 156 1077 186 4247
Fragments arranged by size:
4247 1077 522 259 186 156 113
PmeI GTTT'AAAC
Cuts at: 3559 3559
Size: 6560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1078 1110 5006 5038 1078
Size: 32 3896 32 2600
Fragments arranged by size:
3896 2600 32 32
PpuMI rG'GwC_Cy
Cuts at: 495 1763 1897 2166 2693 3083 495
Size: 1268 134 269 527 390 3972
Fragments arranged by size:
3972 1268 527 390 269 134
PspOMI G'GGCC_C
Cuts at: 2766 2766
Size: 6560
PstI C_TGCA'G
Cuts at: 1029 1736 1782 2706 2886 3607 4292 1029
Size: 707 46 924 180 721 685 3297
Fragments arranged by size:
3297 924 721 707 685 180 46
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6560
PvuII CAG'CTG
Cuts at: 2125 2707 3985 4345 2125
Size: 582 1278 360 4340
Fragments arranged by size:
4340 1278 582 360
RsrII CG'GwC_CG
Cuts at: 4755 4755
Size: 6560
SacI G_AGCT'C
Cuts at: 3574 3574
Size: 6560
SalI G'TCGA_C
Cuts at: 3597 3597
Size: 6560
SanDI GG'GwC_CC
Cuts at: 495 2166 2693 3083 495
Size: 1671 527 390 3972
Fragments arranged by size:
3972 1671 527 390
SapI GCTCTTCn'nnn_
Cuts at: 4583 4793 4583
Size: 210 6350
SbfI CC_TGCA'GG
Cuts at: 3607 3607
Size: 6560
ScaI AGT'ACT
Cuts at: 131 6077 131
Size: 5946 614
SexAI A'CCwGG_T
Cuts at: 1055 2483 1055
Size: 1428 5132
SfcI C'TryA_G
Cuts at: 33 1025 1732 1778 2702 2882 3603 4288 5256
Size: 992 707 46 924 180 721 685 968
Cuts at: 5256 5447 33
Size: 191 1146
Fragments arranged by size:
1146 992 968 924 721 707 685 191
180 46
SfoI GGC'GCC
Cuts at: 3240 3992 4240 3240
Size: 752 248 5560
Fragments arranged by size:
5560 752 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6560
SgrAI Cr'CCGG_yG
Cuts at: 2263 2263
Size: 6560
SmaI CCC'GGG
Cuts at: 1084 1487 2582 3582 1084
Size: 403 1095 1000 4062
Fragments arranged by size:
4062 1095 1000 403
SmlI C'TyrA_G
Cuts at: 3715 4630 5344 5606 3715
Size: 915 714 262 4669
Fragments arranged by size:
4669 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6560
SphI G_CATG'C
Cuts at: 3613 4113 3613
Size: 500 6060
SrfI GCCC'GGGC
Cuts at: 2582 2582
Size: 6560
StyI C'CwwG_G
Cuts at: 107 314 486 549 995 1068 1214 1823 2114
Size: 207 172 63 446 73 146 609 291
Cuts at: 2114 2334 2342 3248 3694 4048 107
Size: 220 8 906 446 354 2619
Fragments arranged by size:
2619 906 609 446 446 354 291 220
207 172 146 73 63 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4143 4276 4735 4944 4143
Size: 133 459 209 5759
Fragments arranged by size:
5759 459 209 133
TatI w'GTAC_w
Cuts at: 129 1187 6075 129
Size: 1058 4888 614
Fragments arranged by size:
4888 1058 614
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2338 2370 2788 2820 6077 6109 2338
Size: 32 418 32 3257 32 2789
Fragments arranged by size:
3257 2789 418 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4357 4357
Size: 6560
XbaI T'CTAG_A
Cuts at: 3591 3591
Size: 6560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 478 1221 3429 4055 478
Size: 743 2208 626 2983
Fragments arranged by size:
2983 2208 743 626
XmaI C'CCGG_G
Cuts at: 1082 1485 2580 3580 1082
Size: 403 1095 1000 4062
Fragments arranged by size:
4062 1095 1000 403
XmnI GAAnn'nnTTC
Cuts at: 248 323 1237 248
Size: 75 914 5571
Fragments arranged by size:
5571 914 75
ZraI GAC'GTC
Cuts at: 1428 1428
Size: 6560
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmI BspEI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SfiI SnaBI SspI StuI
SwaI XhoI