Restriction map for
pF1KB4489
(Circular) (Six-base) MAPSORT of: pF1KB4489.seq Check: 3251 from: 1 to: 4514
pF1KB4489 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 336 336
Size: 4514
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1484 2339 2771 3124 3786 448
Size: 1036 855 432 353 662 1176
Fragments arranged by size:
1176 1036 855 662 432 353
AflIII A'CryG_T
Cuts at: 692 861 1866 3666 3947 692
Size: 169 1005 1800 281 1259
Fragments arranged by size:
1800 1259 1005 281 169
AleI CACnn'nnGTG
Cuts at: 1446 1446
Size: 4514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 382 1192 382
Size: 810 3704
AlwNI CAG_nnn'CTG
Cuts at: 1040 1244 1287 1452 1507 1819 3257 1040
Size: 204 43 165 55 312 1438 2297
Fragments arranged by size:
2297 1438 312 204 165 55 43
ApaLI G'TGCA_C
Cuts at: 711 1114 3352 711
Size: 403 2238 1873
Fragments arranged by size:
2238 1873 403
ApoI r'AATT_y
Cuts at: 1518 3867 4123 1518
Size: 2349 256 1909
Fragments arranged by size:
2349 1909 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 291 1534 291
Size: 1243 3271
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 1530 1944 2192 2227 1530
Size: 414 248 35 3817
Fragments arranged by size:
3817 414 248 35
BanII G_rGCy'C
Cuts at: 282 917 1480 1528 2558 282
Size: 635 563 48 1030 2238
Fragments arranged by size:
2238 1030 635 563 48
BbeI G_GCGC'C
Cuts at: 1948 2196 1948
Size: 248 4266
BbsI GAAGACnn'nnnn_
Cuts at: 1251 1251
Size: 4514
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4480
BciVI GTATCCnnnnn_n'
Cuts at: 2412 3468 4514 2412
Size: 1056 1046 2412
Fragments arranged by size:
2412 1056 1046
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 568 1280 1626 568
Size: 712 346 3456
Fragments arranged by size:
3456 712 346
Bme1580I G_kGCm'C
Cuts at: 715 1118 2139 2232 3356 715
Size: 403 1021 93 1124 1873
Fragments arranged by size:
1873 1124 1021 403 93
BmrI ACTGGGnnnn_n'
Cuts at: 696 1287 1726 1807 2142 4286 696
Size: 591 439 81 335 2144 924
Fragments arranged by size:
2144 924 591 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 457 500 292
Size: 165 43 4306
Fragments arranged by size:
4306 165 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 1690 2569 3283 3581 1237
Size: 453 879 714 298 2170
Fragments arranged by size:
2170 879 714 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 814 4218 814
Size: 3404 1110
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 1945 2193 2895 4391 1945
Size: 248 702 1496 2068
Fragments arranged by size:
2068 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1703 2224 2902 3313 3460 3734 1349
Size: 354 521 678 411 147 274 2129
Fragments arranged by size:
2129 678 521 411 354 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 715 745 682
Size: 30 3 30 4451
Fragments arranged by size:
4451 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 925 925
Size: 4514
BseYI C'CCAG_C
Cuts at: 301 563 685 917 1215 1237 1939 2697 3362
Size: 262 122 232 298 22 702 758 665
Cuts at: 3362 3770 301
Size: 408 1045
Fragments arranged by size:
1045 758 702 665 408 298 262 232
122 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 653 1198 586
Size: 67 545 3902
Fragments arranged by size:
3902 545 67
BsiEI CG_ry'CG
Cuts at: 104 935 2056 2102 3332 104
Size: 831 1121 46 1230 1286
Fragments arranged by size:
1286 1230 1121 831 46
BsiHKAI G_wGCw'C
Cuts at: 536 667 715 931 998 1118 1528 2306 2496
Size: 131 48 216 67 120 410 778 190
Cuts at: 2496 3356 536
Size: 860 1694
Fragments arranged by size:
1694 860 778 410 216 190 131 120
67 48
Bsp1286I G_dGCh'C
Cuts at: 282 536 667 715 917 931 998 1118 1480
Size: 254 131 48 202 14 67 120 362
Cuts at: 1480 1528 2139 2232 2306 2496 2558 3356 282
Size: 48 611 93 74 190 62 798 1440
Fragments arranged by size:
1440 798 611 362 254 202 190 131
120 93 74 67 62 48 48 14
BspEI T'CCGG_A
Cuts at: 1349 1349
Size: 4514
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 1564 1778 2080 2461 336
Size: 1228 214 302 381 2389
Fragments arranged by size:
2389 1228 381 302 214
BsrBI CCG'CTC
Cuts at: 50 326 2806 2860 4344 4509 50
Size: 276 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 276 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2426 2426
Size: 4514
BsrFI r'CCGG_y
Cuts at: 2512 2693 2512
Size: 181 4333
BssSI C'ACGA_G
Cuts at: 421 535 2785 3493 421
Size: 114 2250 708 1442
Fragments arranged by size:
2250 1442 708 114
BstAPI GCAn_nnn'nTGC
Cuts at: 1412 1891 1412
Size: 479 4035
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 1448 1448
Size: 4514
BstYI r'GATC_y
Cuts at: 1 405 1232 1539 2006 2029 2364 2610 2916
Size: 404 827 307 467 23 335 246 306
Cuts at: 2916 2928 3014 4066 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 827 467 449 404 335 307 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 260 1305 260
Size: 1045 3469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1372 2464 2645 2808 1372
Size: 1092 181 163 3078
Fragments arranged by size:
3078 1092 181 163
BtsI GCAGTG_nn'
Cuts at: 164 224 1441 1453 1757 164
Size: 60 1217 12 304 2921
Fragments arranged by size:
2921 1217 304 60 12
ClaI AT'CG_AT
Cuts at: 874 874
Size: 4514
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3564 2220
Size: 1344 3170
EaeI y'GGCC_r
Cuts at: 400 578 716 831 2099 2273 2664 2691 4036
Size: 178 138 115 1268 174 391 27 1345
Cuts at: 4036 400
Size: 878
Fragments arranged by size:
1345 1268 878 391 178 174 138 115
27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 142 157 188 1279 2537 2747 142
Size: 15 31 1091 1258 210 1909
Fragments arranged by size:
1909 1258 1091 210 31 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3464 3610 4324 2722
Size: 742 146 714 2912
Fragments arranged by size:
2912 742 714 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 448 457 500 1484 2339 2771 3124 3786
Size: 156 9 43 984 855 432 353 662
Cuts at: 3786 292
Size: 1020
Fragments arranged by size:
1020 984 855 662 432 353 156 43
9
EcoO109I rG'GnC_Cy
Cuts at: 363 1653 363
Size: 1290 3224
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 399 596 1948 2196 3426 4197 399
Size: 197 1352 248 1230 771 716
Fragments arranged by size:
1352 1230 771 716 248 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 369 397 401 429 750 782 1464 1496 2356
Size: 28 4 28 321 32 682 32 860
Cuts at: 2356 2386 2388 2418 369
Size: 30 2 30 2465
Fragments arranged by size:
2465 860 682 321 32 32 30 30
28 28 4 2
HincII GTy'rAC
Cuts at: 780 1553 780
Size: 773 3741
KasI G'GCGC_C
Cuts at: 1944 2192 1944
Size: 248 4266
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 692 3947 692
Size: 3255 1259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3273 3457 3273
Size: 184 4330
MscI TGG'CCA
Cuts at: 580 833 2275 580
Size: 253 1442 2819
Fragments arranged by size:
2819 1442 253
MslI CAynn'nnrTG
Cuts at: 1174 1433 1446 2503 2630 1174
Size: 259 13 1057 127 3058
Fragments arranged by size:
3058 1057 259 127 13
MspA1I CmG'CkG
Cuts at: 287 435 853 1241 1625 1939 2299 3083 3328
Size: 148 418 388 384 314 360 784 245
Cuts at: 3328 287
Size: 1473
Fragments arranged by size:
1473 784 418 388 384 360 314 245
148
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 1945 2193 1945
Size: 248 4266
NcoI C'CATG_G
Cuts at: 260 260
Size: 4514
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 865 1567 2067 3670 865
Size: 702 500 1603 1709
Fragments arranged by size:
1709 1603 702 500
PciI A'CATG_T
Cuts at: 861 3666 861
Size: 2805 1709
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 4482
PstI C_TGCA'G
Cuts at: 347 413 1249 1448 1561 2246 347
Size: 66 836 199 113 685 2615
Fragments arranged by size:
2615 836 685 199 113 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 287 853 1939 2299 287
Size: 566 1086 360 2502
Fragments arranged by size:
2502 1086 566 360
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 1279 2537 2747 1279
Size: 1258 210 3046
Fragments arranged by size:
3046 1258 210
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 521 4031 521
Size: 3510 1004
SfcI C'TryA_G
Cuts at: 33 343 409 839 1245 1444 1557 2242 3210
Size: 310 66 430 406 199 113 685 968
Cuts at: 3210 3401 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 430 406 310 199 191
113 66
SfoI GGC'GCC
Cuts at: 1946 2194 1946
Size: 248 4266
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 293 1536 293
Size: 1243 3271
SmlI C'TyrA_G
Cuts at: 1252 1669 2584 3298 3560 1252
Size: 417 915 714 262 2206
Fragments arranged by size:
2206 915 714 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1567 2067 1567
Size: 500 4014
StyI C'CwwG_G
Cuts at: 260 1648 2002 260
Size: 1388 354 2772
Fragments arranged by size:
2772 1388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 699 921 1229 2097 2230 2689 2898 699
Size: 222 308 868 133 459 209 2315
Fragments arranged by size:
2315 868 459 308 222 209 133
TatI w'GTAC_w
Cuts at: 519 1121 1342 4029 519
Size: 602 221 2687 1004
Fragments arranged by size:
2687 1004 602 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 1097 1129 4031 4063 682
Size: 32 383 32 2902 32 1133
Fragments arranged by size:
2902 1133 383 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 267 2009 267
Size: 1742 2772
XmaI C'CCGG_G
Cuts at: 291 1534 291
Size: 1243 3271
XmnI GAAnn'nnTTC
Cuts at: 744 1036 744
Size: 292 4222
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI