Restriction map for
pF1KB4506
(Circular) (Six-base) MAPSORT of: pF1KB4506.seq Check: 7790 from: 1 to: 5078
pF1KB4506 5078 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2116 2116
Size: 5078
Acc65I G'GTAC_C
Cuts at: 630 2094 630
Size: 1464 3614
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2903 3335 3688 4350 1769
Size: 1134 432 353 662 2497
Fragments arranged by size:
2497 1134 662 432 353
AflIII A'CryG_T
Cuts at: 2430 4230 4511 2430
Size: 1800 281 2997
Fragments arranged by size:
2997 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1516 1516
Size: 5078
AlwNI CAG_nnn'CTG
Cuts at: 508 661 2383 3821 508
Size: 153 1722 1438 1765
Fragments arranged by size:
1765 1722 1438 153
ApaI G_GGCC'C
Cuts at: 1967 1967
Size: 5078
ApaLI G'TGCA_C
Cuts at: 3916 3916
Size: 5078
ApoI r'AATT_y
Cuts at: 2082 4431 4687 2082
Size: 2349 256 2473
Fragments arranged by size:
2473 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5078
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 1610 1619 1779 2098 1610
Size: 9 160 319 4590
Fragments arranged by size:
4590 319 160 9
AvrII C'CTAG_G
Cuts at: 594 594
Size: 5078
BamHI G'GATC_C
Cuts at: 841 2103 841
Size: 1262 3816
BanI G'GyrC_C
Cuts at: 630 2094 2508 2756 2791 630
Size: 1464 414 248 35 2917
Fragments arranged by size:
2917 1464 414 248 35
BanII G_rGCy'C
Cuts at: 1967 2092 3122 1967
Size: 125 1030 3923
Fragments arranged by size:
3923 1030 125
BbeI G_GCGC'C
Cuts at: 2512 2760 2512
Size: 248 4830
BbsI GAAGACnn'nnnn_
Cuts at: 1178 1364 1950 1178
Size: 186 586 4306
Fragments arranged by size:
4306 586 186
BciVI GTATCCnnnnn_n'
Cuts at: 2976 4032 5078 2976
Size: 1056 1046 2976
Fragments arranged by size:
2976 1056 1046
BclI T'GATC_A
Cuts at: 717 2598 717
Size: 1881 3197
BglI GCCn_nnn'nGGC
Cuts at: 358 1811 358
Size: 1453 3625
BglII A'GATC_T
Cuts at: 1137 2593 4630 1137
Size: 1456 2037 1585
Fragments arranged by size:
2037 1585 1456
BlpI GC'TnA_GC
Cuts at: 2190 2190
Size: 5078
Bme1580I G_kGCm'C
Cuts at: 1967 2703 2796 3920 1967
Size: 736 93 1124 3125
Fragments arranged by size:
3125 1124 736 93
BmgBI CAC'GTC
Cuts at: 2011 2011
Size: 5078
BmrI ACTGGGnnnn_n'
Cuts at: 917 1292 2290 2371 2706 4850 917
Size: 375 998 81 335 2144 1145
Fragments arranged by size:
2144 1145 998 375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1852 3445 1852
Size: 1593 3485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 1106 1655 1682 1731 974
Size: 132 549 27 49 4321
Fragments arranged by size:
4321 549 132 49 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 2254 3133 3847 4145 170
Size: 2084 879 714 298 1103
Fragments arranged by size:
2084 1103 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4782 4782
Size: 5078
BsaAI yAC'GTr
Cuts at: 2431 2431
Size: 5078
BsaBI GATnn'nnATC
Cuts at: 6 1142 1300 2592 4629 6
Size: 1136 158 1292 2037 455
Fragments arranged by size:
2037 1292 1136 455 158
BsaHI Gr'CG_yC
Cuts at: 112 2509 2757 3459 4955 112
Size: 2397 248 702 1496 235
Fragments arranged by size:
2397 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 964 980 2267 2788 3466 3877 4024 4298 964
Size: 16 1287 521 678 411 147 274 1744
Fragments arranged by size:
1744 1287 678 521 411 274 147 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 651 681 975 1005 1496 1526 1631 1661 651
Size: 30 294 30 491 30 105 30 4068
Fragments arranged by size:
4068 491 294 105 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1076 1592 1601 1652 2018 1076
Size: 516 9 51 366 4136
Fragments arranged by size:
4136 516 366 51 9
BseYI C'CCAG_C
Cuts at: 176 589 2503 3261 3926 4334 176
Size: 413 1914 758 665 408 920
Fragments arranged by size:
1914 920 758 665 413 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 647 494
Size: 153 4925
BsiEI CG_ry'CG
Cuts at: 104 2620 2666 3896 104
Size: 2516 46 1230 1286
Fragments arranged by size:
2516 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2045 2092 2870 3060 3920 2045
Size: 47 778 190 860 3203
Fragments arranged by size:
3203 860 778 190 47
Bsp1286I G_dGCh'C
Cuts at: 1967 2045 2092 2703 2796 2870 3060 3122 3920
Size: 78 47 611 93 74 190 62 798
Cuts at: 3920 1967
Size: 3125
Fragments arranged by size:
3125 798 611 190 93 78 74 62
47
BspEI T'CCGG_A
Cuts at: 964 964
Size: 5078
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2128 2342 2644 3025 2128
Size: 214 302 381 4181
Fragments arranged by size:
4181 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3370 3424 4908 5073 50
Size: 3320 54 1484 165 55
Fragments arranged by size:
3320 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2990 2990
Size: 5078
BsrFI r'CCGG_y
Cuts at: 3076 3257 3076
Size: 181 4897
BsrGI T'GTAC_A
Cuts at: 1197 1197
Size: 5078
BssSI C'ACGA_G
Cuts at: 3349 4057 3349
Size: 708 4370
BstAPI GCAn_nnn'nTGC
Cuts at: 508 2455 508
Size: 1947 3131
BstBI TT'CG_AA
Cuts at: 3439 3439
Size: 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 214 385 634 930 214
Size: 171 249 296 4362
Fragments arranged by size:
4362 296 249 171
BstYI r'GATC_y
Cuts at: 1 841 1137 1161 2103 2570 2593 2928 3174
Size: 840 296 24 942 467 23 335 246
Cuts at: 3174 3480 3492 3578 4630 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 942 840 467 449 335 306 296
246 86 24 23 12
BtgI C'CryG_G
Cuts at: 107 116 1121 1382 1515 1553 1572 1878 1902
Size: 9 1005 261 133 38 19 306 24
Cuts at: 1902 107
Size: 3283
Fragments arranged by size:
3283 1005 306 261 133 38 24 19
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1352 3028 3209 3372 1352
Size: 1676 181 163 3058
Fragments arranged by size:
3058 1676 181 163
BtsI GCAGTG_nn'
Cuts at: 278 410 506 659 1258 1367 1678 2321 278
Size: 132 96 153 599 109 311 643 3035
Fragments arranged by size:
3035 643 599 311 153 132 109 96
DraI TTT'AAA
Cuts at: 2077 2077
Size: 5078
DraIII CAC_nnn'GTG
Cuts at: 1486 1516 1486
Size: 30 5048
DrdI GACnn_nn'nnGTC
Cuts at: 1556 2784 4128 1556
Size: 1228 1344 2506
Fragments arranged by size:
2506 1344 1228
EaeI y'GGCC_r
Cuts at: 1875 1908 2663 2837 3228 3255 4600 1875
Size: 33 755 174 391 27 1345 2353
Fragments arranged by size:
2353 1345 755 391 174 33 27
EagI C'GGCC_G
Cuts at: 2663 2663
Size: 5078
EarI CTCTTCn'nnn_
Cuts at: 1011 1821 1995 3101 3311 1011
Size: 810 174 1106 210 2778
Fragments arranged by size:
2778 1106 810 210 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1763 3286 4028 4174 4888 1763
Size: 1523 742 146 714 1953
Fragments arranged by size:
1953 1523 742 714 146
EcoICRI GAG'CTC
Cuts at: 2090 2090
Size: 5078
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 1106 1655 1682 1731 1769 2903 3335 3688
Size: 132 549 27 49 38 1134 432 353
Cuts at: 3688 4350 974
Size: 662 1702
Fragments arranged by size:
1702 1134 662 549 432 353 132 49
38 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1312 1601 1312
Size: 289 4789
EcoO109I rG'GnC_Cy
Cuts at: 1540 1549 1606 1737 1963 1964 2017 2217 1540
Size: 9 57 131 226 1 53 200 4401
Fragments arranged by size:
4401 226 200 131 57 53 9 1
EcoRI G'AATT_C
Cuts at: 2082 2082
Size: 5078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1450 3097 1450
Size: 1647 3431
FspI TGC'GCA
Cuts at: 2859 2859
Size: 5078
HaeII r_GCGC'y
Cuts at: 1085 2512 2760 3990 4761 1085
Size: 1427 248 1230 771 1402
Fragments arranged by size:
1427 1402 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1159 1191 1631 1663 2920 2950 2952 2982 1159
Size: 32 440 32 1257 30 2 30 3255
Fragments arranged by size:
3255 1257 440 32 32 30 30 2
HincII GTy'rAC
Cuts at: 556 790 2117 556
Size: 234 1327 3517
Fragments arranged by size:
3517 1327 234
KasI G'GCGC_C
Cuts at: 2508 2756 2508
Size: 248 4830
KpnI G_GTAC'C
Cuts at: 634 2098 634
Size: 1464 3614
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 370 933 3837 4021 370
Size: 563 2904 184 1427
Fragments arranged by size:
2904 1427 563 184
MscI TGG'CCA
Cuts at: 2839 2839
Size: 5078
MslI CAynn'nnrTG
Cuts at: 3067 3194 3067
Size: 127 4951
MspA1I CmG'CkG
Cuts at: 2189 2503 2863 3647 3892 2189
Size: 314 360 784 245 3375
Fragments arranged by size:
3375 784 360 314 245
NaeI GCC'GGC
Cuts at: 3259 3259
Size: 5078
NarI GG'CG_CC
Cuts at: 2509 2757 2509
Size: 248 4830
NcoI C'CATG_G
Cuts at: 107 1121 1553 107
Size: 1014 432 3632
Fragments arranged by size:
3632 1014 432
NdeI CA'TA_TG
Cuts at: 213 459 213
Size: 246 4832
NgoMIV G'CCGG_C
Cuts at: 3257 3257
Size: 5078
NspI r_CATG'y
Cuts at: 1503 1818 2131 2631 4234 1503
Size: 315 313 500 1603 2347
Fragments arranged by size:
2347 1603 500 315 313
PasI CC'CwG_GG
Cuts at: 1957 1957
Size: 5078
PciI A'CATG_T
Cuts at: 4230 4230
Size: 5078
PflMI CCAn_nnn'nTGG
Cuts at: 487 1516 487
Size: 1029 4049
PfoI T'CCnGG_A
Cuts at: 1524 1524
Size: 5078
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 5046
PpuMI rG'GwC_Cy
Cuts at: 1540 1549 1606 2017 1540
Size: 9 57 411 4601
Fragments arranged by size:
4601 411 57 9
PspOMI G'GGCC_C
Cuts at: 1963 1963
Size: 5078
PstI C_TGCA'G
Cuts at: 147 2125 2810 147
Size: 1978 685 2415
Fragments arranged by size:
2415 1978 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5078
PvuII CAG'CTG
Cuts at: 2503 2863 2503
Size: 360 4718
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 2092 2092
Size: 5078
SalI G'TCGA_C
Cuts at: 2115 2115
Size: 5078
SanDI GG'GwC_CC
Cuts at: 1549 1549
Size: 5078
SapI GCTCTTCn'nnn_
Cuts at: 3101 3311 3101
Size: 210 4868
SbfI CC_TGCA'GG
Cuts at: 2125 2125
Size: 5078
ScaI AGT'ACT
Cuts at: 4595 4595
Size: 5078
SfcI C'TryA_G
Cuts at: 33 132 143 159 416 615 846 1157 2121
Size: 99 11 16 257 199 231 311 964
Cuts at: 2121 2806 3774 3965 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 964 685 311 257 231 199
191 99 16 11
SfoI GGC'GCC
Cuts at: 2510 2758 2510
Size: 248 4830
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 1612 1781 2100 1612
Size: 169 319 4590
Fragments arranged by size:
4590 319 169
SmlI C'TyrA_G
Cuts at: 185 2233 3148 3862 4124 185
Size: 2048 915 714 262 1139
Fragments arranged by size:
2048 1139 915 714 262
SpeI A'CTAG_T
Cuts at: 67 550 709 820 67
Size: 483 159 111 4325
Fragments arranged by size:
4325 483 159 111
SphI G_CATG'C
Cuts at: 1503 1818 2131 2631 1503
Size: 315 313 500 3950
Fragments arranged by size:
3950 500 315 313
StuI AGG'CCT
Cuts at: 436 436
Size: 5078
StyI C'CwwG_G
Cuts at: 107 594 1121 1373 1553 2212 2566 107
Size: 487 527 252 180 659 354 2619
Fragments arranged by size:
2619 659 527 487 354 252 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 445 922 1380 1937 2661 2794 3253 3462
Size: 225 477 458 557 724 133 459 209
Cuts at: 3462 220
Size: 1836
Fragments arranged by size:
1836 724 557 477 459 458 225 209
133
TatI w'GTAC_w
Cuts at: 1197 4593 1197
Size: 3396 1682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 651 683 975 1007 1496 1528 4595 4627 651
Size: 32 292 32 489 32 3067 32 1102
Fragments arranged by size:
3067 1102 489 292 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2875 2875
Size: 5078
XbaI T'CTAG_A
Cuts at: 1224 2109 1224
Size: 885 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1380 1560 1657 2573 1380
Size: 180 97 916 3885
Fragments arranged by size:
3885 916 180 97
XmaI C'CCGG_G
Cuts at: 1610 1779 2098 1610
Size: 169 319 4590
Fragments arranged by size:
4590 319 169
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI