Restriction map for
pF1KB4508
(Circular) (Six-base) MAPSORT of: pF1KB4508.seq Check: 4878 from: 1 to: 4280
pF1KB4508 4280 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1318 1318
Size: 4280
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1238 2105 2537 3284 3552 1238
Size: 867 432 747 268 1966
Fragments arranged by size:
1966 867 747 432 268
AflIII A'CryG_T
Cuts at: 1632 2736 3713 1632
Size: 1104 977 2199
Fragments arranged by size:
2199 1104 977
AgeI A'CCGG_T
Cuts at: 223 2668 223
Size: 2445 1835
AleI CACnn'nnGTG
Cuts at: 490 873 490
Size: 383 3897
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 350
Size: 32 4248
AlwNI CAG_nnn'CTG
Cuts at: 1585 3152 1585
Size: 1567 2713
ApaLI G'TGCA_C
Cuts at: 3050 3050
Size: 4280
ApoI r'AATT_y
Cuts at: 600 1173 1284 3633 3889 600
Size: 573 111 2349 256 991
Fragments arranged by size:
2349 991 573 256 111
AseI AT'TA_AT
Cuts at: 20 454 20
Size: 434 3846
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 1300 1300
Size: 4280
BamHI G'GATC_C
Cuts at: 1305 1305
Size: 4280
BanI G'GyrC_C
Cuts at: 1296 1710 1958 1993 1296
Size: 414 248 35 3583
Fragments arranged by size:
3583 414 248 35
BanII G_rGCy'C
Cuts at: 387 470 1294 2324 387
Size: 83 824 1030 2343
Fragments arranged by size:
2343 1030 824 83
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 624 624
Size: 4280
BbvCI CC'TCA_GC
Cuts at: 1100 1100
Size: 4280
BciVI GTATCCnnnnn_n'
Cuts at: 2178 2939 4280 2178
Size: 761 1341 2178
Fragments arranged by size:
2178 1341 761
BclI T'GATC_A
Cuts at: 1800 1800
Size: 4280
BglII A'GATC_T
Cuts at: 803 1795 3832 803
Size: 992 2037 1251
Fragments arranged by size:
2037 1251 992
BlpI GC'TnA_GC
Cuts at: 436 610 1392 436
Size: 174 782 3324
Fragments arranged by size:
3324 782 174
Bme1580I G_kGCm'C
Cuts at: 765 1056 1905 1998 3054 765
Size: 291 849 93 1056 1991
Fragments arranged by size:
1991 1056 849 291 93
BmrI ACTGGGnnnn_n'
Cuts at: 1492 1573 1908 4052 1492
Size: 81 335 2144 1720
Fragments arranged by size:
2144 1720 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 787 259
Size: 528 3752
Bpu10I CC'TnA_GC
Cuts at: 1100 1100
Size: 4280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 1456 2335 2827 3125 573
Size: 883 879 492 298 1728
Fragments arranged by size:
1728 883 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 417 3984 417
Size: 3567 713
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 223 1469 1990 2668 2942 3089 3500 223
Size: 1246 521 678 274 147 411 1003
Fragments arranged by size:
1246 1003 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 717 747 717
Size: 30 4250
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 852 852
Size: 4280
BseYI C'CCAG_C
Cuts at: 399 903 1705 2463 3040 3536 399
Size: 504 802 758 577 496 1143
Fragments arranged by size:
1143 802 758 577 504 496
BsiEI CG_ry'CG
Cuts at: 104 1822 1868 3076 104
Size: 1718 46 1208 1308
Fragments arranged by size:
1718 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 1294 2072 2262 3054 275
Size: 1019 778 190 792 1501
Fragments arranged by size:
1501 1019 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 275 387 470 765 1056 1294 1905 1998 2072
Size: 112 83 295 291 238 611 93 74
Cuts at: 2072 2262 2324 3054 275
Size: 190 62 730 1501
Fragments arranged by size:
1501 730 611 295 291 238 190 112
93 83 74 62
BspEI T'CCGG_A
Cuts at: 3500 3500
Size: 4280
BspHI T'CATG_A
Cuts at: 1160 3456 1160
Size: 2296 1984
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1247 1330 1544 1846 2227 1247
Size: 83 214 302 381 3300
Fragments arranged by size:
3300 381 302 214 83
BsrBI CCG'CTC
Cuts at: 50 2572 2626 4110 4275 50
Size: 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 898 989 1001 2192 898
Size: 91 12 1191 2986
Fragments arranged by size:
2986 1191 91 12
BsrFI r'CCGG_y
Cuts at: 223 2278 2459 2668 223
Size: 2055 181 209 1835
Fragments arranged by size:
2055 1835 209 181
BssSI C'ACGA_G
Cuts at: 2551 2909 2551
Size: 358 3922
BstAPI GCAn_nnn'nTGC
Cuts at: 1657 1657
Size: 4280
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 423 492 423
Size: 69 4211
BstYI r'GATC_y
Cuts at: 1 803 1305 1772 1795 2130 2376 3388 3474
Size: 802 502 467 23 335 246 1012 86
Cuts at: 3474 3486 3832 1
Size: 12 346 449
Fragments arranged by size:
1012 802 502 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 266 823 107
Size: 159 557 3564
Fragments arranged by size:
3564 557 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2230 2411 2574 2230
Size: 181 163 3936
Fragments arranged by size:
3936 181 163
BtsI GCAGTG_nn'
Cuts at: 1523 1523
Size: 4280
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 628 1986 2844 628
Size: 1358 858 2064
Fragments arranged by size:
2064 1358 858
EaeI y'GGCC_r
Cuts at: 1139 1865 2039 2430 2457 3802 1139
Size: 726 174 391 27 1345 1617
Fragments arranged by size:
1617 1345 726 391 174 27
EagI C'GGCC_G
Cuts at: 1865 1865
Size: 4280
EarI CTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 2798 2944 4090 2488
Size: 310 146 1146 2678
Fragments arranged by size:
2678 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 787 1238 2105 2537 3284 3552 259
Size: 528 451 867 432 747 268 987
Fragments arranged by size:
987 867 747 528 451 432 268
EcoO109I rG'GnC_Cy
Cuts at: 621 1419 621
Size: 798 3482
EcoRI G'AATT_C
Cuts at: 600 1284 600
Size: 684 3596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 1018 1714 1962 2984 3963 1018
Size: 696 248 1022 979 1335
Fragments arranged by size:
1335 1022 979 696 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2122 2152 2154 2184 2122
Size: 30 2 30 4218
Fragments arranged by size:
4218 30 30 2
HincII GTy'rAC
Cuts at: 324 1319 324
Size: 995 3285
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1086 1202 2951 3135 1086
Size: 116 1749 184 2231
Fragments arranged by size:
2231 1749 184 116
MscI TGG'CCA
Cuts at: 1141 2041 1141
Size: 900 3380
MslI CAynn'nnrTG
Cuts at: 421 490 873 994 2269 2396 421
Size: 69 383 121 1275 127 2305
Fragments arranged by size:
2305 1275 383 127 121 69
MspA1I CmG'CkG
Cuts at: 916 1391 1705 2065 3078 3323 916
Size: 475 314 360 1013 245 1873
Fragments arranged by size:
1873 1013 475 360 314 245
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NcoI C'CATG_G
Cuts at: 107 823 107
Size: 716 3564
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NspI r_CATG'y
Cuts at: 886 1333 1833 2740 886
Size: 447 500 907 2426
Fragments arranged by size:
2426 907 500 447
PciI A'CATG_T
Cuts at: 2736 2736
Size: 4280
PflMI CCAn_nnn'nTGG
Cuts at: 1038 1038
Size: 4280
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4248
PpuMI rG'GwC_Cy
Cuts at: 621 621
Size: 4280
PstI C_TGCA'G
Cuts at: 1327 2012 1327
Size: 685 3595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 1705 2065 1705
Size: 360 3920
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 500 3797 500
Size: 3297 983
SexAI A'CCwGG_T
Cuts at: 167 167
Size: 4280
SfcI C'TryA_G
Cuts at: 33 728 1323 2008 3001 3192 33
Size: 695 595 685 993 191 1121
Fragments arranged by size:
1121 993 695 685 595 191
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SgrAI Cr'CCGG_yG
Cuts at: 223 223
Size: 4280
SmaI CCC'GGG
Cuts at: 1302 1302
Size: 4280
SmlI C'TyrA_G
Cuts at: 588 1435 2350 2842 3104 588
Size: 847 915 492 262 1764
Fragments arranged by size:
1764 915 847 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 886 1333 1833 886
Size: 447 500 3333
Fragments arranged by size:
3333 500 447
StyI C'CwwG_G
Cuts at: 107 823 1414 1768 107
Size: 716 591 354 2619
Fragments arranged by size:
2619 716 591 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1863 1996 2455 2664 1863
Size: 133 459 209 3479
Fragments arranged by size:
3479 459 209 133
TatI w'GTAC_w
Cuts at: 498 3795 498
Size: 3297 983
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4248
Tth111I GACn'n_nGTC
Cuts at: 923 2077 923
Size: 1154 3126
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 743 1121 1254 1775 743
Size: 378 133 521 3248
Fragments arranged by size:
3248 521 378 133
XmaI C'CCGG_G
Cuts at: 1300 1300
Size: 4280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI