Restriction map for
pF1KB4514
(Circular) (Six-base) MAPSORT of: pF1KB4514.seq Check: 3057 from: 1 to: 7973
pF1KB4514 7973 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1178 2728 3206 1178
Size: 1550 478 5945
Fragments arranged by size:
5945 1550 478
AccI GT'mk_AC
Cuts at: 305 3083 4181 5011 305
Size: 2778 1098 830 3267
Fragments arranged by size:
3267 2778 1098 830
Acc65I G'GTAC_C
Cuts at: 4812 4989 4812
Size: 177 7796
AclI AA'CG_TT
Cuts at: 4739 7813 4739
Size: 3074 4899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 508 1393 3451 3587 4238 5798 6230 6977
Size: 141 885 2058 136 651 1560 432 747
Cuts at: 6977 7245 367
Size: 268 1095
Fragments arranged by size:
2058 1560 1095 885 747 651 432 268
141 136
AflIII A'CryG_T
Cuts at: 1796 2167 2669 4530 5325 6429 7406 1796
Size: 371 502 1861 795 1104 977 2363
Fragments arranged by size:
2363 1861 1104 977 795 502 371
AgeI A'CCGG_T
Cuts at: 6361 6361
Size: 7973
AhdI GACnn_n'nnGTC
Cuts at: 3720 4044 3720
Size: 324 7649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2819 2819
Size: 7973
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 429 461 1621 1653 3581 3613 429
Size: 32 1160 32 1928 32 4789
Fragments arranged by size:
4789 1928 1160 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1203 1928 3074 3303 3869 3892 5278 6845 1203
Size: 725 1146 229 566 23 1386 1567 2331
Fragments arranged by size:
2331 1567 1386 1146 725 566 229 23
ApaI G_GGCC'C
Cuts at: 578 2186 2263 4967 578
Size: 1608 77 2704 3584
Fragments arranged by size:
3584 2704 1608 77
ApaLI G'TGCA_C
Cuts at: 1159 6743 1159
Size: 5584 2389
ApoI r'AATT_y
Cuts at: 2112 3625 4977 7326 7582 2112
Size: 1513 1352 2349 256 2503
Fragments arranged by size:
2503 2349 1513 1352 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7973
AvaI C'yCGr_G
Cuts at: 227 311 361 580 679 2104 2316 3750 3797
Size: 84 50 219 99 1425 212 1434 47
Cuts at: 3797 3929 4993 227
Size: 132 1064 3207
Fragments arranged by size:
3207 1434 1425 1064 219 212 132 99
84 50 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4804 4837 4804
Size: 33 7940
BamHI G'GATC_C
Cuts at: 665 1960 4588 4998 665
Size: 1295 2628 410 3640
Fragments arranged by size:
3640 2628 1295 410
BanI G'GyrC_C
Cuts at: 285 420 977 1166 2289 2613 2803 3438 4812
Size: 135 557 189 1123 324 190 635 1374
Cuts at: 4812 4989 5403 5651 5686 285
Size: 177 414 248 35 2572
Fragments arranged by size:
2572 1374 1123 635 557 414 324 248
190 189 177 135 35
BanII G_rGCy'C
Cuts at: 125 169 199 578 1426 1744 1757 2186 2263
Size: 44 30 379 848 318 13 429 77
Cuts at: 2263 3334 3701 4784 4967 4987 6017 125
Size: 1071 367 1083 183 20 1030 2081
Fragments arranged by size:
2081 1083 1071 1030 848 429 379 367
318 183 77 44 30 20 13
BbeI G_GCGC'C
Cuts at: 424 981 5407 5655 424
Size: 557 4426 248 2742
Fragments arranged by size:
4426 2742 557 248
BbsI GAAGACnn'nnnn_
Cuts at: 3562 4226 3562
Size: 664 7309
BbvCI CC'TCA_GC
Cuts at: 1083 3152 1083
Size: 2069 5904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3778 3812 3778
Size: 34 7939
BciVI GTATCCnnnnn_n'
Cuts at: 4917 5871 6632 7973 4917
Size: 954 761 1341 4917
Fragments arranged by size:
4917 1341 954 761
BclI T'GATC_A
Cuts at: 2621 3614 5493 2621
Size: 993 1879 5101
Fragments arranged by size:
5101 1879 993
BglI GCCn_nnn'nGGC
Cuts at: 1975 2656 3241 4347 1975
Size: 681 585 1106 5601
Fragments arranged by size:
5601 1106 681 585
BglII A'GATC_T
Cuts at: 761 2279 5488 7525 761
Size: 1518 3209 2037 1209
Fragments arranged by size:
3209 2037 1518 1209
BlpI GC'TnA_GC
Cuts at: 3346 5085 3346
Size: 1739 6234
Bme1580I G_kGCm'C
Cuts at: 290 578 1163 1494 2186 2263 2618 4967 5598
Size: 288 585 331 692 77 355 2349 631
Cuts at: 5598 5691 6747 290
Size: 93 1056 1516
Fragments arranged by size:
2349 1516 1056 692 631 585 355 331
288 93 77
BmrI ACTGGGnnnn_n'
Cuts at: 2453 3001 4757 5185 5266 5601 7745 2453
Size: 548 1756 428 81 335 2144 2681
Fragments arranged by size:
2681 2144 1756 548 428 335 81
BmtI G_CTAG'C
Cuts at: 382 382
Size: 7973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1017 1249 2715 6340 1017
Size: 232 1466 3625 2650
Fragments arranged by size:
3625 2650 1466 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 589 919 949 1232 1258 1314 1438 2422
Size: 33 330 30 283 26 56 124 984
Cuts at: 2422 2710 3715 4144 4732 4925 556
Size: 288 1005 429 588 193 3604
Fragments arranged by size:
3604 1005 984 588 429 330 288 283
193 124 56 33 30 26
Bpu10I CC'TnA_GC
Cuts at: 403 1083 2236 3152 403
Size: 680 1153 916 5224
Fragments arranged by size:
5224 1153 916 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1806 1896 3703 5149 6028 6520 6818 1806
Size: 90 1807 1446 879 492 298 2961
Fragments arranged by size:
2961 1807 1446 879 492 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 435 3707 3730 4389 4494 7677 435
Size: 3272 23 659 105 3183 731
Fragments arranged by size:
3272 3183 731 659 105 23
BsaAI yAC'GTr
Cuts at: 2586 3792 4533 5326 2586
Size: 1206 741 793 5233
Fragments arranged by size:
5233 1206 793 741
BsaBI GATnn'nnATC
Cuts at: 6 5487 7524 6
Size: 5481 2037 455
Fragments arranged by size:
5481 2037 455
BsaHI Gr'CG_yC
Cuts at: 421 691 978 1290 2450 3161 5404 5652 6354
Size: 270 287 312 1160 711 2243 248 702
Cuts at: 6354 7850 421
Size: 1496 544
Fragments arranged by size:
2243 1496 1160 711 702 544 312 287
270 248
BsaWI w'CCGG_w
Cuts at: 2728 2809 3514 4184 5162 5683 6361 6635 6782
Size: 81 705 670 978 521 678 274 147
Cuts at: 6782 7193 2728
Size: 411 3508
Fragments arranged by size:
3508 978 705 678 670 521 411 274
147 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1116 1146 1975 2005 2212 2242 2597 2627 1116
Size: 30 829 30 207 30 355 30 6462
Fragments arranged by size:
6462 829 355 207 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 327 562 1009 1137 1592 1897 2576 2579 2691
Size: 235 447 128 455 305 679 3 112
Cuts at: 2691 2883 2918 3405 3753 4364 4420 4423 4440
Size: 192 35 487 348 611 56 3 17
Cuts at: 4440 327
Size: 3860
Fragments arranged by size:
3860 679 611 487 455 447 348 305
235 192 128 112 56 35 17 3
3
BseYI C'CCAG_C
Cuts at: 201 411 540 740 1196 2385 3009 4099 4419
Size: 210 129 200 456 1189 624 1090 320
Cuts at: 4419 5398 6156 6733 7229 201
Size: 979 758 577 496 945
Fragments arranged by size:
1189 1090 979 945 758 624 577 496
456 320 210 200 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 924 1125 1712 3772 4248 924
Size: 201 587 2060 476 4649
Fragments arranged by size:
4649 2060 587 476 201
BsiEI CG_ry'CG
Cuts at: 104 4517 5515 5561 6769 104
Size: 4413 998 46 1208 1308
Fragments arranged by size:
4413 1308 1208 998 46
BsiHKAI G_wGCw'C
Cuts at: 1139 1163 1274 1426 1744 1757 2893 3577 3701
Size: 24 111 152 318 13 1136 684 124
Cuts at: 3701 4987 5765 5955 6747 1139
Size: 1286 778 190 792 2365
Fragments arranged by size:
2365 1286 1136 792 778 684 318 190
152 124 111 24 13
BsiWI C'GTAC_G
Cuts at: 4625 4625
Size: 7973
BsmI GAATG_Cn'
Cuts at: 3524 4543 3524
Size: 1019 6954
BsmBI CGTCTCn'nnnn_
Cuts at: 1158 3168 1158
Size: 2010 5963
Bsp1286I G_dGCh'C
Cuts at: 125 169 199 290 578 1139 1163 1274 1426
Size: 44 30 91 288 561 24 111 152
Cuts at: 1426 1494 1744 1757 2186 2263 2618 2893 3334
Size: 68 250 13 429 77 355 275 441
Cuts at: 3334 3577 3701 4784 4967 4987 5598 5691 5765
Size: 243 124 1083 183 20 611 93 74
Cuts at: 5765 5955 6017 6747 125
Size: 190 62 730 1351
Fragments arranged by size:
1351 1083 730 611 561 441 429 355
288 275 250 243 190 183 152 124
111 93 91 77 74 68 62 44
30 24 20 13
BspEI T'CCGG_A
Cuts at: 2728 3514 7193 2728
Size: 786 3679 3508
Fragments arranged by size:
3679 3508 786
BspHI T'CATG_A
Cuts at: 3272 3371 7149 3272
Size: 99 3778 4096
Fragments arranged by size:
4096 3778 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1178 1602 2728 3206 5023 5237 5539 5920 1178
Size: 424 1126 478 1817 214 302 381 3231
Fragments arranged by size:
3231 1817 1126 478 424 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6265 6319 7803 7968 50
Size: 6215 54 1484 165 55
Fragments arranged by size:
6215 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1190 2624 2934 3803 3854 5885 1190
Size: 1434 310 869 51 2031 3278
Fragments arranged by size:
3278 2031 1434 869 310 51
BsrFI r'CCGG_y
Cuts at: 181 417 442 2610 4512 5971 6152 6361 181
Size: 236 25 2168 1902 1459 181 209 1793
Fragments arranged by size:
2168 1902 1793 1459 236 209 181 25
BsrGI T'GTAC_A
Cuts at: 2420 3002 2420
Size: 582 7391
BssHII G'CGCG_C
Cuts at: 189 189
Size: 7973
BssSI C'ACGA_G
Cuts at: 3238 4690 6244 6602 3238
Size: 1452 1554 358 4609
Fragments arranged by size:
4609 1554 1452 358
BstAPI GCAn_nnn'nTGC
Cuts at: 531 3312 3675 5350 531
Size: 2781 363 1675 3154
Fragments arranged by size:
3154 2781 1675 363
BstBI TT'CG_AA
Cuts at: 6334 6334
Size: 7973
BstEII G'GTnAC_C
Cuts at: 3225 4699 3225
Size: 1474 6499
BstXI CCAn_nnnn'nTGG
Cuts at: 528 2001 3318 3989 4245 528
Size: 1473 1317 671 256 4256
Fragments arranged by size:
4256 1473 1317 671 256
BstYI r'GATC_y
Cuts at: 1 665 761 1229 1960 2036 2279 4588 4998
Size: 664 96 468 731 76 243 2309 410
Cuts at: 4998 5465 5488 5823 6069 7081 7167 7179 7525
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7525 1
Size: 449
Fragments arranged by size:
2309 1012 731 664 468 467 449 410
346 335 246 243 96 86 76 23
12
BstZ17I GTA'TAC
Cuts at: 4182 4182
Size: 7973
Bsu36I CC'TnA_GG
Cuts at: 955 2895 4361 955
Size: 1940 1466 4567
Fragments arranged by size:
4567 1940 1466
BtgI C'CryG_G
Cuts at: 107 152 369 722 1114 2187 2370 3033 3135
Size: 45 217 353 392 1073 183 663 102
Cuts at: 3135 3442 3477 4517 4584 107
Size: 307 35 1040 67 3496
Fragments arranged by size:
3496 1073 1040 663 392 353 307 217
183 102 67 45 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 865 5923 6104 6267 865
Size: 5058 181 163 2571
Fragments arranged by size:
5058 2571 181 163
BtsI GCAGTG_nn'
Cuts at: 943 1137 1361 1389 1894 4768 5216 943
Size: 194 224 28 505 2874 448 3700
Fragments arranged by size:
3700 2874 505 448 224 194 28
DraI TTT'AAA
Cuts at: 3017 4972 3017
Size: 1955 6018
DraIII CAC_nnn'GTG
Cuts at: 2376 2376
Size: 7973
DrdI GACnn_nn'nnGTC
Cuts at: 5679 6537 5679
Size: 858 7115
EaeI y'GGCC_r
Cuts at: 183 1148 3242 3770 3902 4514 5558 5732 6123
Size: 965 2094 528 132 612 1044 174 391
Cuts at: 6123 6150 7495 183
Size: 27 1345 661
Fragments arranged by size:
2094 1345 1044 965 661 612 528 391
174 132 27
EagI C'GGCC_G
Cuts at: 4514 5558 4514
Size: 1044 6929
EarI CTCTTCn'nnn_
Cuts at: 256 301 760 3891 4319 4325 4791 5996 6206
Size: 45 459 3131 428 6 466 1205 210
Cuts at: 6206 256
Size: 2023
Fragments arranged by size:
3131 2023 1205 466 459 428 210 45
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 658 2573 2924 6181 6491 6637 7783 658
Size: 1915 351 3257 310 146 1146 848
Fragments arranged by size:
3257 1915 1146 848 351 310 146
EcoICRI GAG'CTC
Cuts at: 1424 1742 1755 3699 4985 1424
Size: 318 13 1944 1286 4412
Fragments arranged by size:
4412 1944 1286 318 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 508 556 589 919 949 1232 1258 1314
Size: 141 48 33 330 30 283 26 56
Cuts at: 1314 1393 1438 2422 2710 3451 3587 3715 4144
Size: 79 45 984 288 741 136 128 429
Cuts at: 4144 4238 4732 4925 5798 6230 6977 7245 367
Size: 94 494 193 873 432 747 268 1095
Fragments arranged by size:
1095 984 873 747 741 494 432 429
330 288 283 268 193 141 136 128
94 79 56 48 45 33 30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 1182 2404 3433 1182
Size: 1222 1029 5722
Fragments arranged by size:
5722 1222 1029
EcoO109I rG'GnC_Cy
Cuts at: 315 575 1619 2183 2259 2260 2648 3889 4272
Size: 260 1044 564 76 1 388 1241 383
Cuts at: 4272 4356 4763 5112 315
Size: 84 407 349 3176
Fragments arranged by size:
3176 1241 1044 564 407 388 383 349
260 84 76 1
EcoRI G'AATT_C
Cuts at: 4977 4977
Size: 7973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5992 5992
Size: 7973
FseI GG_CCGG'CC
Cuts at: 4516 4516
Size: 7973
FspI TGC'GCA
Cuts at: 505 5754 505
Size: 5249 2724
HaeII r_GCGC'y
Cuts at: 424 981 5407 5655 6677 7656 424
Size: 557 4426 248 1022 979 741
Fragments arranged by size:
4426 1022 979 741 557 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 249 281 650 682 1230 1262 1975 2007 3148
Size: 32 369 32 548 32 713 32 1141
Cuts at: 3148 3180 3706 3729 3738 3761 4030 4062 4179
Size: 32 526 23 9 23 269 32 117
Cuts at: 4179 4211 5815 5845 5847 5877 249
Size: 32 1604 30 2 30 2345
Fragments arranged by size:
2345 1604 1141 713 548 526 369 269
117 32 32 32 32 32 32 32
30 30 23 23 9 2
HincII GTy'rAC
Cuts at: 276 5012 276
Size: 4736 3237
KasI G'GCGC_C
Cuts at: 420 977 5403 5651 420
Size: 557 4426 248 2742
Fragments arranged by size:
4426 2742 557 248
KpnI G_GTAC'C
Cuts at: 4816 4993 4816
Size: 177 7796
MluI A'CGCG_T
Cuts at: 7406 7406
Size: 7973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 674 1243 1417 2255 4580 6644 6828 674
Size: 569 174 838 2325 2064 184 1819
Fragments arranged by size:
2325 2064 1819 838 569 184 174
MscI TGG'CCA
Cuts at: 3772 3904 5734 3772
Size: 132 1830 6011
Fragments arranged by size:
6011 1830 132
MslI CAynn'nnrTG
Cuts at: 2359 3277 3316 3658 5962 6089 2359
Size: 918 39 342 2304 127 4243
Fragments arranged by size:
4243 2304 918 342 127 39
MspA1I CmG'CkG
Cuts at: 141 160 205 219 371 399 724 1087 1200
Size: 19 45 14 152 28 325 363 113
Cuts at: 1200 2180 3444 4423 4480 5084 5398 5758 6771
Size: 980 1264 979 57 604 314 360 1013
Cuts at: 6771 7016 141
Size: 245 1098
Fragments arranged by size:
1264 1098 1013 980 979 604 363 360
325 314 245 152 113 57 45 28
19 14
NaeI GCC'GGC
Cuts at: 419 4514 6154 419
Size: 4095 1640 2238
Fragments arranged by size:
4095 2238 1640
NarI GG'CG_CC
Cuts at: 421 978 5404 5652 421
Size: 557 4426 248 2742
Fragments arranged by size:
4426 2742 557 248
NcoI C'CATG_G
Cuts at: 107 2187 3477 107
Size: 2080 1290 4603
Fragments arranged by size:
4603 2080 1290
NgoMIV G'CCGG_C
Cuts at: 417 4512 6152 417
Size: 4095 1640 2238
Fragments arranged by size:
4095 2238 1640
NheI G'CTAG_C
Cuts at: 378 378
Size: 7973
NspI r_CATG'y
Cuts at: 807 1858 2171 2418 5026 5526 6433 807
Size: 1051 313 247 2608 500 907 2347
Fragments arranged by size:
2608 2347 1051 907 500 313 247
PasI CC'CwG_GG
Cuts at: 1494 4171 1494
Size: 2677 5296
PciI A'CATG_T
Cuts at: 2167 6429 2167
Size: 4262 3711
PflMI CCAn_nnn'nTGG
Cuts at: 3892 4613 3892
Size: 721 7252
PfoI T'CCnGG_A
Cuts at: 169 433 1640 169
Size: 264 1207 6502
Fragments arranged by size:
6502 1207 264
PmeI GTTT'AAAC
Cuts at: 4972 4972
Size: 7973
PmlI CAC'GTG
Cuts at: 3792 4533 3792
Size: 741 7232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7112 7144 7112
Size: 32 7941
PpuMI rG'GwC_Cy
Cuts at: 315 1619 4272 4356 315
Size: 1304 2653 84 3932
Fragments arranged by size:
3932 2653 1304 84
PshAI GACnn'nnGTC
Cuts at: 3089 3089
Size: 7973
PspOMI G'GGCC_C
Cuts at: 574 2182 2259 4963 574
Size: 1608 77 2704 3584
Fragments arranged by size:
3584 2704 1608 77
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4609 4641 4609
Size: 32 7941
PstI C_TGCA'G
Cuts at: 224 257 1175 1356 1384 1421 1937 2045 3070
Size: 33 918 181 28 37 516 108 1025
Cuts at: 3070 3329 4485 5020 5705 224
Size: 259 1156 535 685 2492
Fragments arranged by size:
2492 1156 1025 918 685 535 516 259
181 108 37 33 28
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7973
PvuII CAG'CTG
Cuts at: 160 205 1087 1200 4423 4480 5398 5758 160
Size: 45 882 113 3223 57 918 360 2375
Fragments arranged by size:
3223 2375 918 882 360 113 57 45
RsrII CG'GwC_CG
Cuts at: 6168 6168
Size: 7973
SacI G_AGCT'C
Cuts at: 1426 1744 1757 3701 4987 1426
Size: 318 13 1944 1286 4412
Fragments arranged by size:
4412 1944 1286 318 13
SacII CC_GC'GG
Cuts at: 372 725 3445 372
Size: 353 2720 4900
Fragments arranged by size:
4900 2720 353
SalI G'TCGA_C
Cuts at: 5010 5010
Size: 7973
SapI GCTCTTCn'nnn_
Cuts at: 301 5996 6206 301
Size: 5695 210 2068
Fragments arranged by size:
5695 2068 210
SbfI CC_TGCA'GG
Cuts at: 5020 5020
Size: 7973
ScaI AGT'ACT
Cuts at: 7490 7490
Size: 7973
SfcI C'TryA_G
Cuts at: 33 220 253 1171 1352 1380 1417 1703 1933
Size: 187 33 918 181 28 37 286 230
Cuts at: 1933 2041 2144 2307 3066 3325 3939 3954 4481
Size: 108 103 163 759 259 614 15 527
Cuts at: 4481 4670 5016 5701 6694 6885 33
Size: 189 346 685 993 191 1121
Fragments arranged by size:
1121 993 918 759 685 614 527 346
286 259 230 191 189 187 181 163
108 103 37 33 28 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2656 2656
Size: 7973
SfoI GGC'GCC
Cuts at: 422 979 5405 5653 422
Size: 557 4426 248 2742
Fragments arranged by size:
4426 2742 557 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7973
SmaI CCC'GGG
Cuts at: 229 313 582 2106 4995 229
Size: 84 269 1524 2889 3207
Fragments arranged by size:
3207 2889 1524 269 84
SmlI C'TyrA_G
Cuts at: 1785 1875 3718 5128 6043 6535 6797 1785
Size: 90 1843 1410 915 492 262 2961
Fragments arranged by size:
2961 1843 1410 915 492 262 90
SnaBI TAC'GTA
Cuts at: 2586 2586
Size: 7973
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7973
SphI G_CATG'C
Cuts at: 5026 5526 5026
Size: 500 7473
SspI AAT'ATT
Cuts at: 3048 4022 3048
Size: 974 6999
StuI AGG'CCT
Cuts at: 1000 1468 4006 1000
Size: 468 2538 4967
Fragments arranged by size:
4967 2538 468
StyI C'CwwG_G
Cuts at: 107 645 785 1578 1802 2187 3477 4352 5107
Size: 538 140 793 224 385 1290 875 755
Cuts at: 5107 5461 107
Size: 354 2619
Fragments arranged by size:
2619 1290 875 793 755 538 385 354
224 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2070 2212 2693 3195 3391 4173 4282 5556 5689
Size: 142 481 502 196 782 109 1274 133
Cuts at: 5689 6148 6357 2070
Size: 459 209 3686
Fragments arranged by size:
3686 1274 782 502 481 459 209 196
142 133 109
TatI w'GTAC_w
Cuts at: 2420 2461 3002 3338 4723 7488 2420
Size: 41 541 336 1385 2765 2905
Fragments arranged by size:
2905 2765 1385 541 336 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1116 1148 2597 2629 2981 3013 7490 7522 1116
Size: 32 1449 32 352 32 4477 32 1567
Fragments arranged by size:
4477 1567 1449 352 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 474 5770 474
Size: 5296 2677
XbaI T'CTAG_A
Cuts at: 5004 5004
Size: 7973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 159 1971 2238 3484 5468 159
Size: 1812 267 1246 1984 2664
Fragments arranged by size:
2664 1984 1812 1246 267
XmaI C'CCGG_G
Cuts at: 227 311 580 2104 4993 227
Size: 84 269 1524 2889 3207
Fragments arranged by size:
3207 2889 1524 269 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BfrBI BmgBI
ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PsiI PspXI SanDI SexAI
SgrAI SrfI SwaI XhoI XmnI ZraI