Restriction map for
pF1KB4523
(Circular) (Six-base) MAPSORT of: pF1KB4523.seq Check: 9257 from: 1 to: 4628
pF1KB4523 4628 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 319 1067 319
Size: 748 3880
AccI GT'mk_AC
Cuts at: 656 1666 656
Size: 1010 3618
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 2453 2885 3238 3900 1277
Size: 1176 432 353 662 2005
Fragments arranged by size:
2005 1176 662 432 353
AflIII A'CryG_T
Cuts at: 239 241 1980 3780 4061 239
Size: 2 1739 1800 281 806
Fragments arranged by size:
1800 1739 806 281 2
AgeI A'CCGG_T
Cuts at: 992 992
Size: 4628
AhdI GACnn_n'nnGTC
Cuts at: 1116 1116
Size: 4628
AleI CACnn'nnGTG
Cuts at: 939 939
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 201 591 679 1198 1461 1933 3371 201
Size: 390 88 519 263 472 1438 1458
Fragments arranged by size:
1458 1438 519 472 390 263 88
ApaI G_GGCC'C
Cuts at: 185 478 185
Size: 293 4335
ApaLI G'TGCA_C
Cuts at: 213 3466 213
Size: 3253 1375
ApoI r'AATT_y
Cuts at: 1632 3981 4237 1632
Size: 2349 256 2023
Fragments arranged by size:
2349 2023 256
AseI AT'TA_AT
Cuts at: 20 1277 20
Size: 1257 3371
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 185 1324 1648 185
Size: 1139 324 3165
Fragments arranged by size:
3165 1139 324
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 429 925 1078 1644 2058 2306 2341 429
Size: 496 153 566 414 248 35 2716
Fragments arranged by size:
2716 566 496 414 248 153 35
BanII G_rGCy'C
Cuts at: 185 367 478 1642 2672 185
Size: 182 111 1164 1030 2141
Fragments arranged by size:
2141 1164 1030 182 111
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 1588 1616 1588
Size: 28 4600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 399 433 399
Size: 34 4594
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3582 4628 2526
Size: 1056 1046 2526
Fragments arranged by size:
2526 1056 1046
BclI T'GATC_A
Cuts at: 1452 2148 1452
Size: 696 3932
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1597 1740 1597
Size: 143 4485
Bme1580I G_kGCm'C
Cuts at: 185 217 434 478 1081 1412 2253 2346 3470
Size: 32 217 44 603 331 841 93 1124
Cuts at: 3470 185
Size: 1343
Fragments arranged by size:
1343 1124 841 603 331 217 93 44
32
BmgBI CAC'GTC
Cuts at: 1614 1614
Size: 4628
BmrI ACTGGGnnnn_n'
Cuts at: 428 1840 1921 2256 4400 428
Size: 1412 81 335 2144 656
Fragments arranged by size:
2144 1412 656 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 628 804 1385 211
Size: 417 176 581 3454
Fragments arranged by size:
3454 581 417 176
Bpu10I CC'TnA_GC
Cuts at: 361 455 684 361
Size: 94 229 4305
Fragments arranged by size:
4305 229 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 444 1804 2683 3397 3695 430
Size: 14 1360 879 714 298 1363
Fragments arranged by size:
1363 1360 879 714 298 14
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 242 1981 242
Size: 1739 2889
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 992 1817 2338 3016 3427 3574 3848 992
Size: 825 521 678 411 147 274 1772
Fragments arranged by size:
1772 825 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 777 807 1279 1309 649
Size: 30 98 30 472 30 3968
Fragments arranged by size:
3968 472 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 1382 120
Size: 1262 3366
BseYI C'CCAG_C
Cuts at: 1592 2053 2811 3476 3884 1592
Size: 461 758 665 408 2336
Fragments arranged by size:
2336 758 665 461 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1624 1045
Size: 579 4049
BsiEI CG_ry'CG
Cuts at: 104 122 2170 2216 3446 104
Size: 18 2048 46 1230 1286
Fragments arranged by size:
2048 1286 1230 46 18
BsiHKAI G_wGCw'C
Cuts at: 217 299 367 461 968 1642 2420 2610 3470
Size: 82 68 94 507 674 778 190 860
Cuts at: 3470 217
Size: 1375
Fragments arranged by size:
1375 860 778 674 507 190 94 82
68
Bsp1286I G_dGCh'C
Cuts at: 185 217 299 367 434 461 478 968 1081
Size: 32 82 68 67 27 17 490 113
Cuts at: 1081 1412 1642 2253 2346 2420 2610 2672 3470
Size: 331 230 611 93 74 190 62 798
Cuts at: 3470 185
Size: 1343
Fragments arranged by size:
1343 798 611 490 331 230 190 113
93 82 74 68 67 62 32 27
17
BspHI T'CATG_A
Cuts at: 960 1185 3060 960
Size: 225 1875 2528
Fragments arranged by size:
2528 1875 225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 319 383 1067 1678 1892 2194 2575 319
Size: 64 684 611 214 302 381 2372
Fragments arranged by size:
2372 684 611 381 302 214 64
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1211 2540 1211
Size: 1329 3299
BsrFI r'CCGG_y
Cuts at: 163 502 992 2626 2807 163
Size: 339 490 1634 181 1984
Fragments arranged by size:
1984 1634 490 339 181
BssSI C'ACGA_G
Cuts at: 821 2899 3607 821
Size: 2078 708 1842
Fragments arranged by size:
2078 1842 708
BstAPI GCAn_nnn'nTGC
Cuts at: 221 303 2005 221
Size: 82 1702 2844
Fragments arranged by size:
2844 1702 82
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstEII G'GTnAC_C
Cuts at: 439 689 439
Size: 250 4378
BstYI r'GATC_y
Cuts at: 1 603 648 1653 2120 2143 2478 2724 3030
Size: 602 45 1005 467 23 335 246 306
Cuts at: 3030 3042 3128 4180 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1005 602 467 449 335 306 246
86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 672 672
Size: 4628
BtgI C'CryG_G
Cuts at: 107 107
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 483 2578 2759 2922 483
Size: 2095 181 163 2189
Fragments arranged by size:
2189 2095 181 163
BtsI GCAGTG_nn'
Cuts at: 387 1501 1871 387
Size: 1114 370 3144
Fragments arranged by size:
3144 1114 370
DraI TTT'AAA
Cuts at: 1302 1627 1302
Size: 325 4303
DraIII CAC_nnn'GTG
Cuts at: 303 303
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3678 2334
Size: 1344 3284
EaeI y'GGCC_r
Cuts at: 119 2213 2387 2778 2805 4150 119
Size: 2094 174 391 27 1345 597
Fragments arranged by size:
2094 1345 597 391 174 27
EagI C'GGCC_G
Cuts at: 119 2213 119
Size: 2094 2534
EarI CTCTTCn'nnn_
Cuts at: 822 2651 2861 822
Size: 1829 210 2589
Fragments arranged by size:
2589 1829 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2836 3578 3724 4438 127
Size: 2709 742 146 714 317
Fragments arranged by size:
2709 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 365 1640 365
Size: 1275 3353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 628 804 1277 1385 2453 2885 3238 3900
Size: 417 176 473 108 1068 432 353 662
Cuts at: 3900 211
Size: 939
Fragments arranged by size:
1068 939 662 473 432 417 353 176
108
EcoO109I rG'GnC_Cy
Cuts at: 182 257 372 474 971 1767 182
Size: 75 115 102 497 796 3043
Fragments arranged by size:
3043 796 497 115 102 75
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1047 2647 1047
Size: 1600 3028
FspI TGC'GCA
Cuts at: 307 379 1573 2409 307
Size: 72 1194 836 2526
Fragments arranged by size:
2526 1194 836 72
FspAI rTGC'GCAy
Cuts at: 307 307
Size: 4628
HaeII r_GCGC'y
Cuts at: 2062 2310 3540 4311 2062
Size: 248 1230 771 2379
Fragments arranged by size:
2379 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 955 987 1102 1134 2470 2500 2502
Size: 32 475 32 115 32 1336 30 2
Cuts at: 2502 2532 448
Size: 30 2544
Fragments arranged by size:
2544 1336 475 115 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MfeI C'AATT_G
Cuts at: 1007 1007
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3387 3571 3387
Size: 184 4444
MscI TGG'CCA
Cuts at: 2389 2389
Size: 4628
MslI CAynn'nnrTG
Cuts at: 566 939 1132 1279 2617 2744 566
Size: 373 193 147 1338 127 2450
Fragments arranged by size:
2450 1338 373 193 147 127
MspA1I CmG'CkG
Cuts at: 170 221 782 939 1389 1739 2053 2413 3197
Size: 51 561 157 450 350 314 360 784
Cuts at: 3197 3442 170
Size: 245 1356
Fragments arranged by size:
1356 784 561 450 360 350 314 245
157 51
NaeI GCC'GGC
Cuts at: 165 504 2809 165
Size: 339 2305 1984
Fragments arranged by size:
2305 1984 339
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 107 107
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 163 502 2807 163
Size: 339 2305 1984
Fragments arranged by size:
2305 1984 339
NspI r_CATG'y
Cuts at: 1152 1681 2181 3784 1152
Size: 529 500 1603 1996
Fragments arranged by size:
1996 1603 529 500
PciI A'CATG_T
Cuts at: 3780 3780
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 591 1484 591
Size: 893 3735
PfoI T'CCnGG_A
Cuts at: 644 644
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PmlI CAC'GTG
Cuts at: 242 242
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 372 971 372
Size: 599 4029
PspOMI G'GGCC_C
Cuts at: 181 474 181
Size: 293 4335
PstI C_TGCA'G
Cuts at: 1675 2360 1675
Size: 685 3943
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 221 939 1389 2053 2413 221
Size: 718 450 664 360 2436
Fragments arranged by size:
2436 718 664 450 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 367 1642 367
Size: 1275 3353
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SanDI GG'GwC_CC
Cuts at: 971 971
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 1262 4145 1262
Size: 2883 1745
SfcI C'TryA_G
Cuts at: 33 1671 2356 3324 3515 33
Size: 1638 685 968 191 1146
Fragments arranged by size:
1638 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 187 1650 187
Size: 1463 3165
SmlI C'TyrA_G
Cuts at: 423 445 1783 2698 3412 3674 423
Size: 22 1338 915 714 262 1377
Fragments arranged by size:
1377 1338 915 714 262 22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
SspI AAT'ATT
Cuts at: 812 812
Size: 4628
StyI C'CwwG_G
Cuts at: 107 891 1083 1762 2116 107
Size: 784 192 679 354 2619
Fragments arranged by size:
2619 784 679 354 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 400 1096 2211 2344 2803 3012 400
Size: 696 1115 133 459 209 2016
Fragments arranged by size:
2016 1115 696 459 209 133
TatI w'GTAC_w
Cuts at: 1260 4143 1260
Size: 2883 1745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 777 809 1279 1311 4145 4177 777
Size: 32 470 32 2834 32 1228
Fragments arranged by size:
2834 1228 470 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 279 327 1090 1164 1245 2123 279
Size: 48 763 74 81 878 2784
Fragments arranged by size:
2784 878 763 81 74 48
XmaI C'CCGG_G
Cuts at: 185 1648 185
Size: 1463 3165
XmnI GAAnn'nnTTC
Cuts at: 349 811 349
Size: 462 4166
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoNI EcoRV
FseI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI