Restriction map for
pF1KB4538
(Circular) (Six-base) MAPSORT of: pF1KB4538.seq Check: 9550 from: 1 to: 4304
pF1KB4538 4304 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 123 123
Size: 4304
AccI GT'mk_AC
Cuts at: 1342 1342
Size: 4304
Acc65I G'GTAC_C
Cuts at: 1320 1320
Size: 4304
AclI AA'CG_TT
Cuts at: 4144 4144
Size: 4304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 988 1111 1149 2129 2561 3308 3576 793
Size: 195 123 38 980 432 747 268 1521
Fragments arranged by size:
1521 980 747 432 268 195 123 38
AflIII A'CryG_T
Cuts at: 1656 2760 3737 1656
Size: 1104 977 2223
Fragments arranged by size:
2223 1104 977
AgeI A'CCGG_T
Cuts at: 2692 2692
Size: 4304
AleI CACnn'nnGTG
Cuts at: 418 418
Size: 4304
AlwNI CAG_nnn'CTG
Cuts at: 820 1609 3176 820
Size: 789 1567 1948
Fragments arranged by size:
1948 1567 789
ApaLI G'TGCA_C
Cuts at: 3074 3074
Size: 4304
ApoI r'AATT_y
Cuts at: 703 1308 3657 3913 703
Size: 605 2349 256 1094
Fragments arranged by size:
2349 1094 605 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4304
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
AvaI C'yCGr_G
Cuts at: 271 1324 271
Size: 1053 3251
BamHI G'GATC_C
Cuts at: 1329 1329
Size: 4304
BanI G'GyrC_C
Cuts at: 1320 1734 1982 2017 1320
Size: 414 248 35 3607
Fragments arranged by size:
3607 414 248 35
BanII G_rGCy'C
Cuts at: 1318 2348 1318
Size: 1030 3274
BbeI G_GCGC'C
Cuts at: 1738 1986 1738
Size: 248 4056
BbsI GAAGACnn'nnnn_
Cuts at: 156 636 156
Size: 480 3824
BciVI GTATCCnnnnn_n'
Cuts at: 154 2202 2963 4304 154
Size: 2048 761 1341 154
Fragments arranged by size:
2048 1341 761 154
BclI T'GATC_A
Cuts at: 1824 1824
Size: 4304
BglII A'GATC_T
Cuts at: 912 1218 1819 3856 912
Size: 306 601 2037 1360
Fragments arranged by size:
2037 1360 601 306
BlpI GC'TnA_GC
Cuts at: 1416 1416
Size: 4304
Bme1580I G_kGCm'C
Cuts at: 1929 2022 3078 1929
Size: 93 1056 3155
Fragments arranged by size:
3155 1056 93
BmgBI CAC'GTC
Cuts at: 416 416
Size: 4304
BmrI ACTGGGnnnn_n'
Cuts at: 1516 1597 1932 4076 1516
Size: 81 335 2144 1744
Fragments arranged by size:
2144 1744 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4304
Bpu10I CC'TnA_GC
Cuts at: 328 328
Size: 4304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1232 1480 2359 2851 3149 1232
Size: 248 879 492 298 2387
Fragments arranged by size:
2387 879 492 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 1274 4008 1274
Size: 2734 1570
BsaAI yAC'GTr
Cuts at: 1657 1657
Size: 4304
BsaBI GATnn'nnATC
Cuts at: 6 1818 3855 6
Size: 1812 2037 455
Fragments arranged by size:
2037 1812 455
BsaHI Gr'CG_yC
Cuts at: 120 1735 1983 2685 4181 120
Size: 1615 248 702 1496 243
Fragments arranged by size:
1615 1496 702 248 243
BsaWI w'CCGG_w
Cuts at: 1493 2014 2692 2966 3113 3524 1493
Size: 521 678 274 147 411 2273
Fragments arranged by size:
2273 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1192 1222 1192
Size: 30 4274
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1198 1198
Size: 4304
BseYI C'CCAG_C
Cuts at: 651 1729 2487 3064 3560 651
Size: 1078 758 577 496 1395
Fragments arranged by size:
1395 1078 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1846 1892 3100 104
Size: 1742 46 1208 1308
Fragments arranged by size:
1742 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1287 1318 2096 2286 3078 1287
Size: 31 778 190 792 2513
Fragments arranged by size:
2513 792 778 190 31
Bsp1286I G_dGCh'C
Cuts at: 1287 1318 1929 2022 2096 2286 2348 3078 1287
Size: 31 611 93 74 190 62 730 2513
Fragments arranged by size:
2513 730 611 190 93 74 62 31
BspEI T'CCGG_A
Cuts at: 3524 3524
Size: 4304
BspHI T'CATG_A
Cuts at: 3480 3480
Size: 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1354 1568 1870 2251 1354
Size: 214 302 381 3407
Fragments arranged by size:
3407 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2596 2650 4134 4299 50
Size: 2546 54 1484 165 55
Fragments arranged by size:
2546 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2216 2216
Size: 4304
BsrFI r'CCGG_y
Cuts at: 308 2302 2483 2692 308
Size: 1994 181 209 1920
Fragments arranged by size:
1994 1920 209 181
BssSI C'ACGA_G
Cuts at: 2575 2933 2575
Size: 358 3946
BstAPI GCAn_nnn'nTGC
Cuts at: 1681 1681
Size: 4304
BstBI TT'CG_AA
Cuts at: 2665 2665
Size: 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 943 943
Size: 4304
BstYI r'GATC_y
Cuts at: 1 912 1218 1329 1796 1819 2154 2400 3412
Size: 911 306 111 467 23 335 246 1012
Cuts at: 3412 3498 3510 3856 1
Size: 86 12 346 449
Fragments arranged by size:
1012 911 467 449 346 335 306 246
111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 406 1057 406
Size: 651 3653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 854 2254 2435 2598 854
Size: 1400 181 163 2560
Fragments arranged by size:
2560 1400 181 163
BtsI GCAGTG_nn'
Cuts at: 493 1547 493
Size: 1054 3250
DraI TTT'AAA
Cuts at: 456 1292 1303 456
Size: 836 11 3457
Fragments arranged by size:
3457 836 11
DrdI GACnn_nn'nnGTC
Cuts at: 409 2010 2868 409
Size: 1601 858 1845
Fragments arranged by size:
1845 1601 858
EaeI y'GGCC_r
Cuts at: 1044 1889 2063 2454 2481 3826 1044
Size: 845 174 391 27 1345 1522
Fragments arranged by size:
1522 1345 845 391 174 27
EagI C'GGCC_G
Cuts at: 1889 1889
Size: 4304
EarI CTCTTCn'nnn_
Cuts at: 922 2327 2537 922
Size: 1405 210 2689
Fragments arranged by size:
2689 1405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2512 2822 2968 4114 2512
Size: 310 146 1146 2702
Fragments arranged by size:
2702 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1316 1316
Size: 4304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 988 1111 1149 2129 2561 3308 3576 793
Size: 195 123 38 980 432 747 268 1521
Fragments arranged by size:
1521 980 747 432 268 195 123 38
EcoO109I rG'GnC_Cy
Cuts at: 1443 1443
Size: 4304
EcoRI G'AATT_C
Cuts at: 1308 1308
Size: 4304
EcoRV GAT'ATC
Cuts at: 260 1179 260
Size: 919 3385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 748 2323 748
Size: 1575 2729
FspI TGC'GCA
Cuts at: 2085 2085
Size: 4304
HaeII r_GCGC'y
Cuts at: 1738 1986 3008 3987 1738
Size: 248 1022 979 2055
Fragments arranged by size:
2055 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1252 1284 2146 2176 2178 2208 1252
Size: 32 862 30 2 30 3348
Fragments arranged by size:
3348 862 32 30 30 2
HincII GTy'rAC
Cuts at: 1343 1343
Size: 4304
KasI G'GCGC_C
Cuts at: 1734 1982 1734
Size: 248 4056
KpnI G_GTAC'C
Cuts at: 1324 1324
Size: 4304
MluI A'CGCG_T
Cuts at: 3737 3737
Size: 4304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 96 2975 3159 96
Size: 2879 184 1241
Fragments arranged by size:
2879 1241 184
MscI TGG'CCA
Cuts at: 2065 2065
Size: 4304
MslI CAynn'nnrTG
Cuts at: 418 733 2293 2420 418
Size: 315 1560 127 2302
Fragments arranged by size:
2302 1560 315 127
MspA1I CmG'CkG
Cuts at: 1415 1729 2089 3102 3347 1415
Size: 314 360 1013 245 2372
Fragments arranged by size:
2372 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2485 2485
Size: 4304
NarI GG'CG_CC
Cuts at: 1735 1983 1735
Size: 248 4056
NgoMIV G'CCGG_C
Cuts at: 2483 2483
Size: 4304
NspI r_CATG'y
Cuts at: 336 1357 1857 2764 336
Size: 1021 500 907 1876
Fragments arranged by size:
1876 1021 907 500
PciI A'CATG_T
Cuts at: 2760 2760
Size: 4304
PflMI CCAn_nnn'nTGG
Cuts at: 633 633
Size: 4304
PfoI T'CCnGG_A
Cuts at: 590 590
Size: 4304
PmeI GTTT'AAAC
Cuts at: 1303 1303
Size: 4304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 4272
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 298 330 298
Size: 32 4272
PstI C_TGCA'G
Cuts at: 1351 2036 1351
Size: 685 3619
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4304
PvuII CAG'CTG
Cuts at: 1729 2089 1729
Size: 360 3944
RsrII CG'GwC_CG
Cuts at: 2499 2499
Size: 4304
SacI G_AGCT'C
Cuts at: 1318 1318
Size: 4304
SalI G'TCGA_C
Cuts at: 1341 1341
Size: 4304
SapI GCTCTTCn'nnn_
Cuts at: 2327 2537 2327
Size: 210 4094
SbfI CC_TGCA'GG
Cuts at: 1351 1351
Size: 4304
ScaI AGT'ACT
Cuts at: 804 973 3821 804
Size: 169 2848 1287
Fragments arranged by size:
2848 1287 169
SexAI A'CCwGG_T
Cuts at: 1079 1079
Size: 4304
SfcI C'TryA_G
Cuts at: 33 744 1347 2032 3025 3216 33
Size: 711 603 685 993 191 1121
Fragments arranged by size:
1121 993 711 685 603 191
SfoI GGC'GCC
Cuts at: 1736 1984 1736
Size: 248 4056
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
SmaI CCC'GGG
Cuts at: 1326 1326
Size: 4304
SmlI C'TyrA_G
Cuts at: 1247 1459 2374 2866 3128 1247
Size: 212 915 492 262 2423
Fragments arranged by size:
2423 915 492 262 212
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4304
SphI G_CATG'C
Cuts at: 336 1357 1857 336
Size: 1021 500 2783
Fragments arranged by size:
2783 1021 500
StyI C'CwwG_G
Cuts at: 1438 1792 1438
Size: 354 3950
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 877 1887 2020 2479 2688 877
Size: 1010 133 459 209 2493
Fragments arranged by size:
2493 1010 459 209 133
TatI w'GTAC_w
Cuts at: 314 802 971 1230 3819 314
Size: 488 169 259 2589 799
Fragments arranged by size:
2589 799 488 259 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4272
Tth111I GACn'n_nGTC
Cuts at: 1081 2101 1081
Size: 1020 3284
XbaI T'CTAG_A
Cuts at: 1335 1335
Size: 4304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 1799 211
Size: 1588 2716
XmaI C'CCGG_G
Cuts at: 1324 1324
Size: 4304
XmnI GAAnn'nnTTC
Cuts at: 532 532
Size: 4304
ZraI GAC'GTC
Cuts at: 121 121
Size: 4304
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BpmI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI