Restriction map for
pF1KB4546
(Circular) (Six-base) MAPSORT of: pF1KB4546.seq Check: 7982 from: 1 to: 13493
pF1KB4546 13493 bp
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 521 561 3242 3614 4827 521
Size: 40 2681 372 1213 9187
Fragments arranged by size:
9187 2681 1213 372 40
AccI GT'mk_AC
Cuts at: 1772 1976 5297 6485 10531 1772
Size: 204 3321 1188 4046 4734
Fragments arranged by size:
4734 4046 3321 1188 204
Acc65I G'GTAC_C
Cuts at: 3266 6428 10509 3266
Size: 3162 4081 6250
Fragments arranged by size:
6250 4081 3162
AclI AA'CG_TT
Cuts at: 6185 13333 6185
Size: 7148 6345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2324 3417 3556 3618 4054 4640 5118 5582
Size: 640 1093 139 62 436 586 478 464
Cuts at: 5582 6098 6874 8112 8207 8378 8791 8803 9155
Size: 516 776 1238 95 171 413 12 352
Cuts at: 9155 11318 11750 12497 12765 1684
Size: 2163 432 747 268 2412
Fragments arranged by size:
2412 2163 1238 1093 776 747 640 586
516 478 464 436 432 413 352 268
171 139 95 62 12
AfeI AGC'GCT
Cuts at: 149 8158 149
Size: 8009 5484
AflII C'TTAA_G
Cuts at: 8706 8706
Size: 13493
AflIII A'CryG_T
Cuts at: 2090 3212 7556 8201 9779 10845 11949 12926 2090
Size: 1122 4344 645 1578 1066 1104 977 2657
Fragments arranged by size:
4344 2657 1578 1122 1104 1066 977 645
AgeI A'CCGG_T
Cuts at: 1513 9835 11881 1513
Size: 8322 2046 3125
Fragments arranged by size:
8322 3125 2046
AhdI GACnn_n'nnGTC
Cuts at: 4761 4761
Size: 13493
AleI CACnn'nnGTG
Cuts at: 6404 6404
Size: 13493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 836 7153 836
Size: 6317 7176
AlwNI CAG_nnn'CTG
Cuts at: 122 892 2754 3295 3673 4352 5885 7022 8095
Size: 770 1862 541 378 679 1533 1137 1073
Cuts at: 8095 10798 12365 122
Size: 2703 1567 1250
Fragments arranged by size:
2703 1862 1567 1533 1250 1137 1073 770
679 541 378
ApaI G_GGCC'C
Cuts at: 129 6002 8668 129
Size: 5873 2666 4954
Fragments arranged by size:
5873 4954 2666
ApaLI G'TGCA_C
Cuts at: 225 670 2157 6382 12263 225
Size: 445 1487 4225 5881 1455
Fragments arranged by size:
5881 4225 1487 1455 445
ApoI r'AATT_y
Cuts at: 573 1375 1553 1569 1717 2134 2615 3034 4933
Size: 802 178 16 148 417 481 419 1899
Cuts at: 4933 5538 5797 6232 8426 8864 10419 10497 12846
Size: 605 259 435 2194 438 1555 78 2349
Cuts at: 12846 13102 573
Size: 256 964
Fragments arranged by size:
2349 2194 1899 1555 964 802 605 481
438 435 419 417 259 256 178 148
78 16
AseI AT'TA_AT
Cuts at: 20 6658 20
Size: 6638 6855
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 13493
AvaI C'yCGr_G
Cuts at: 3977 7636 9434 10082 10513 3977
Size: 3659 1798 648 431 6957
Fragments arranged by size:
6957 3659 1798 648 431
BamHI G'GATC_C
Cuts at: 4271 6216 7391 8899 9731 10518 4271
Size: 1945 1175 1508 832 787 7246
Fragments arranged by size:
7246 1945 1508 1175 832 787
BanI G'GyrC_C
Cuts at: 2987 3266 3602 4507 5408 5783 6072 6260 6428
Size: 279 336 905 901 375 289 188 168
Cuts at: 6428 7129 7328 7853 9838 10509 10923 11171 11206
Size: 701 199 525 1985 671 414 248 35
Cuts at: 11206 2987
Size: 5274
Fragments arranged by size:
5274 1985 905 901 701 671 525 414
375 336 289 279 248 199 188 168
35
BanII G_rGCy'C
Cuts at: 129 654 1014 2469 2570 4178 6002 7683 8668
Size: 525 360 1455 101 1608 1824 1681 985
Cuts at: 8668 10442 10507 11537 129
Size: 1774 65 1030 2085
Fragments arranged by size:
2085 1824 1774 1681 1608 1455 1030 985
525 360 101 65
BbeI G_GCGC'C
Cuts at: 10927 11175 10927
Size: 248 13245
BbsI GAAGACnn'nnnn_
Cuts at: 1455 4819 6014 6877 7164 8219 8779 10467 1455
Size: 3364 1195 863 287 1055 560 1688 4481
Fragments arranged by size:
4481 3364 1688 1195 1055 863 560 287
BbvCI CC'TCA_GC
Cuts at: 169 169
Size: 13493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4726 4760 8863 8897 4726
Size: 34 4103 34 9322
Fragments arranged by size:
9322 4103 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1629 3468 4721 5007 5976 8728 9451 9817 11391
Size: 1839 1253 286 969 2752 723 366 1574
Cuts at: 11391 12152 13493 1629
Size: 761 1341 1629
Fragments arranged by size:
2752 1839 1629 1574 1341 1253 969 761
723 366 286
BclI T'GATC_A
Cuts at: 1154 2472 9185 10353 11013 1154
Size: 1318 6713 1168 660 3634
Fragments arranged by size:
6713 3634 1318 1168 660
BfrBI ATG'CAT
Cuts at: 813 7580 813
Size: 6767 6726
BglI GCCn_nnn'nGGC
Cuts at: 1135 4873 1135
Size: 3738 9755
BglII A'GATC_T
Cuts at: 1335 1940 5664 5734 7311 8381 11008 13045 1335
Size: 605 3724 70 1577 1070 2627 2037 1783
Fragments arranged by size:
3724 2627 2037 1783 1577 1070 605 70
BlpI GC'TnA_GC
Cuts at: 4480 10605 4480
Size: 6125 7368
Bme1580I G_kGCm'C
Cuts at: 129 229 562 674 2161 3208 4512 5413 6002
Size: 100 333 112 1487 1047 1304 901 589
Cuts at: 6002 6075 6386 7010 8668 9234 9821 11118 11211
Size: 73 311 624 1658 566 587 1297 93
Cuts at: 11211 12267 129
Size: 1056 1355
Fragments arranged by size:
1658 1487 1355 1304 1297 1056 1047 901
624 589 587 566 333 311 112 100
93 73
BmgBI CAC'GTC
Cuts at: 6300 6300
Size: 13493
BmrI ACTGGGnnnn_n'
Cuts at: 1198 2443 3787 4340 4863 6492 6811 8073 8134
Size: 1245 1344 553 523 1629 319 1262 61
Cuts at: 8134 8973 9573 9899 9963 10480 10705 10786 11121
Size: 839 600 326 64 517 225 81 335
Cuts at: 11121 13265 1198
Size: 2144 1426
Fragments arranged by size:
2144 1629 1426 1344 1262 1245 839 600
553 523 517 335 326 319 225 81
64 61
BmtI G_CTAG'C
Cuts at: 3613 3613
Size: 13493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4302 11860 4302
Size: 7558 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 2082 2401 2557 2977 3159 3679 4043 4143
Size: 1273 319 156 420 182 520 364 100
Cuts at: 4143 5143 6387 6961 9345 10309 809
Size: 1000 1244 574 2384 964 3993
Fragments arranged by size:
3993 2384 1273 1244 1000 964 574 520
420 364 319 182 156 100
Bpu10I CC'TnA_GC
Cuts at: 169 5424 169
Size: 5255 8238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2820 3146 9648 10669 11548 12040 12338 2820
Size: 326 6502 1021 879 492 298 3975
Fragments arranged by size:
6502 3975 1021 879 492 326 298
BsaI GGTCTCn'nnnn_
Cuts at: 4244 5056 6749 7633 9210 9952 13197 4244
Size: 812 1693 884 1577 742 3245 4540
Fragments arranged by size:
4540 3245 1693 1577 884 812 742
BsaAI yAC'GTr
Cuts at: 504 2459 7200 9780 10846 504
Size: 1955 4741 2580 1066 3151
Fragments arranged by size:
4741 3151 2580 1955 1066
BsaBI GATnn'nnATC
Cuts at: 6 1334 3959 8933 11007 13044 6
Size: 1328 2625 4974 2074 2037 455
Fragments arranged by size:
4974 2625 2074 2037 1328 455
BsaHI Gr'CG_yC
Cuts at: 1061 2370 3563 7747 10924 11172 11874 13370 1061
Size: 1309 1193 4184 3177 248 702 1496 1184
Fragments arranged by size:
4184 3177 1496 1309 1193 1184 702 248
BsaWI w'CCGG_w
Cuts at: 1513 8598 9835 10682 11203 11881 12155 12302 12713
Size: 7085 1237 847 521 678 274 147 411
Cuts at: 12713 1513
Size: 2293
Fragments arranged by size:
7085 2293 1237 847 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 712 742 1229 1259 1943 1973 3365 3395 4973
Size: 30 487 30 684 30 1392 30 1578
Cuts at: 4973 5003 5304 5334 6830 6860 8143 8173 9688
Size: 30 301 30 1496 30 1283 30 1515
Cuts at: 9688 9718 712
Size: 30 4487
Fragments arranged by size:
4487 1578 1515 1496 1392 1283 684 487
301 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1971 2259 3096 3571 6384 6520 9420 9696 1971
Size: 288 837 475 2813 136 2900 276 5768
Fragments arranged by size:
5768 2900 2813 837 475 288 276 136
BseYI C'CCAG_C
Cuts at: 122 156 629 946 1133 2199 3341 3442 6331
Size: 34 473 317 187 1066 1142 101 2889
Cuts at: 6331 8147 9271 9764 10232 10918 11676 12253 12749
Size: 1816 1124 493 468 686 758 577 496
Cuts at: 12749 122
Size: 866
Fragments arranged by size:
2889 1816 1142 1124 1066 866 758 686
577 496 493 473 468 317 187 101
34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 2481 3237 4397 5594 6382 6805 7581 8455
Size: 1977 756 1160 1197 788 423 776 874
Cuts at: 8455 8867 9813 504
Size: 412 946 4184
Fragments arranged by size:
4184 1977 1197 1160 946 874 788 776
756 423 412
BsiEI CG_ry'CG
Cuts at: 104 9836 11035 11081 12289 104
Size: 9732 1199 46 1208 1308
Fragments arranged by size:
9732 1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 229 275 654 674 2161 2458 5425 6326 6386
Size: 46 379 20 1487 297 2967 901 60
Cuts at: 6386 6848 7683 8237 9225 10507 11285 11475 12267
Size: 462 835 554 988 1282 778 190 792
Cuts at: 12267 229
Size: 1455
Fragments arranged by size:
2967 1487 1455 1282 988 901 835 792
778 554 462 379 297 190 60 46
20
BsmI GAATG_Cn'
Cuts at: 1933 3010 3286 4860 6123 7372 1933
Size: 1077 276 1574 1263 1249 8054
Fragments arranged by size:
8054 1574 1263 1249 1077 276
Bsp1286I G_dGCh'C
Cuts at: 129 229 275 562 654 674 1014 2161 2458
Size: 100 46 287 92 20 340 1147 297
Cuts at: 2458 2469 2570 3208 4178 4512 5413 5425 6002
Size: 11 101 638 970 334 901 12 577
Cuts at: 6002 6075 6326 6386 6848 7010 7683 8237 8668
Size: 73 251 60 462 162 673 554 431
Cuts at: 8668 9225 9234 9821 10442 10507 11118 11211 11285
Size: 557 9 587 621 65 611 93 74
Cuts at: 11285 11475 11537 12267 129
Size: 190 62 730 1355
Fragments arranged by size:
1355 1147 970 901 730 673 638 621
611 587 577 557 554 462 431 340
334 297 287 251 190 162 101 100
93 92 74 73 65 62 60 46
20 12 11 9
BspEI T'CCGG_A
Cuts at: 12713 12713
Size: 13493
BspHI T'CATG_A
Cuts at: 1575 2562 2687 4080 6794 8310 10350 12669 1575
Size: 987 125 1393 2714 1516 2040 2319 2399
Fragments arranged by size:
2714 2399 2319 2040 1516 1393 987 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 561 3242 3614 4675 4805 4827 6917 7937
Size: 40 2681 372 1061 130 22 2090 1020
Cuts at: 7937 10543 10757 11059 11440 521
Size: 2606 214 302 381 2574
Fragments arranged by size:
2681 2606 2574 2090 1061 1020 381 372
302 214 130 40 22
BsrBI CCG'CTC
Cuts at: 50 11785 11839 13323 13488 50
Size: 11735 54 1484 165 55
Fragments arranged by size:
11735 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 825 1369 3941 6924 6932 9198 11405 825
Size: 544 2572 2983 8 2266 2207 2913
Fragments arranged by size:
2983 2913 2572 2266 2207 544 8
BsrFI r'CCGG_y
Cuts at: 1513 6448 8173 9835 11491 11672 11881 1513
Size: 4935 1725 1662 1656 181 209 3125
Fragments arranged by size:
4935 3125 1725 1662 1656 209 181
BsrGI T'GTAC_A
Cuts at: 4325 5151 5674 8257 9782 4325
Size: 826 523 2583 1525 8036
Fragments arranged by size:
8036 2583 1525 826 523
BssHII G'CGCG_C
Cuts at: 175 177 175
Size: 2 13491
BssSI C'ACGA_G
Cuts at: 851 6752 6876 8152 11764 12122 851
Size: 5901 124 1276 3612 358 2222
Fragments arranged by size:
5901 3612 2222 1276 358 124
BstAPI GCAn_nnn'nTGC
Cuts at: 3281 4145 4667 6267 9611 10870 3281
Size: 864 522 1600 3344 1259 5904
Fragments arranged by size:
5904 3344 1600 1259 864 522
BstBI TT'CG_AA
Cuts at: 6626 11854 6626
Size: 5228 8265
BstEII G'GTnAC_C
Cuts at: 469 9508 469
Size: 9039 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 466 761 1839 2736 6467 6921 7771 9279 10071
Size: 295 1078 897 3731 454 850 1508 792
Cuts at: 10071 466
Size: 3888
Fragments arranged by size:
3888 3731 1508 1078 897 850 792 454
295
BstYI r'GATC_y
Cuts at: 1 1335 1940 4271 5664 5734 6216 6409 7311
Size: 1334 605 2331 1393 70 482 193 902
Cuts at: 7311 7391 7803 8381 8899 8934 9731 10074 10518
Size: 80 412 578 518 35 797 343 444
Cuts at: 10518 10985 11008 11343 11589 12601 12687 12699 13045
Size: 467 23 335 246 1012 86 12 346
Cuts at: 13045 1
Size: 449
Fragments arranged by size:
2331 1393 1334 1012 902 797 605 578
518 482 467 449 444 412 346 343
335 246 193 86 80 70 35 23
12
Bsu36I CC'TnA_GG
Cuts at: 1055 1528 5403 1055
Size: 473 3875 9145
Fragments arranged by size:
9145 3875 473
BtgI C'CryG_G
Cuts at: 107 234 1287 1502 1896 2295 2973 4362 6212
Size: 127 1053 215 394 399 678 1389 1850
Cuts at: 6212 7569 7742 8694 10014 107
Size: 1357 173 952 1320 3586
Fragments arranged by size:
3586 1850 1389 1357 1320 1053 952 678
399 394 215 173 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 10212 11443 11624 11787 10212
Size: 1231 181 163 11918
Fragments arranged by size:
11918 1231 181 163
BtsI GCAGTG_nn'
Cuts at: 123 492 3208 3225 4362 5852 7569 8339 8644
Size: 369 2716 17 1137 1490 1717 770 305
Cuts at: 8644 8879 10187 10736 123
Size: 235 1308 549 2880
Fragments arranged by size:
2880 2716 1717 1490 1308 1137 770 549
369 305 235 17
ClaI AT'CG_AT
Cuts at: 3963 5945 7031 8648 3963
Size: 1982 1086 1617 8808
Fragments arranged by size:
8808 1982 1617 1086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1069 1104 3151 3186 1069
Size: 35 2047 35 11376
Fragments arranged by size:
11376 2047 35 35
DraI TTT'AAA
Cuts at: 5631 9474 10492 5631
Size: 3843 1018 8632
Fragments arranged by size:
8632 3843 1018
DraIII CAC_nnn'GTG
Cuts at: 8858 10170 8858
Size: 1312 12181
DrdI GACnn_nn'nnGTC
Cuts at: 11199 12057 11199
Size: 858 12635
EaeI y'GGCC_r
Cuts at: 2330 2790 2916 4359 7566 9391 11078 11252 11643
Size: 460 126 1443 3207 1825 1687 174 391
Cuts at: 11643 11670 13015 2330
Size: 27 1345 2808
Fragments arranged by size:
3207 2808 1825 1687 1443 1345 460 391
174 126 27
EagI C'GGCC_G
Cuts at: 11078 11078
Size: 13493
EarI CTCTTCn'nnn_
Cuts at: 3417 5198 7652 9600 11516 11726 3417
Size: 1781 2454 1948 1916 210 5184
Fragments arranged by size:
5184 2454 1948 1916 1781 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3533 11701 12011 12157 13303 3533
Size: 8168 310 146 1146 3723
Fragments arranged by size:
8168 3723 1146 310 146
EcoICRI GAG'CTC
Cuts at: 652 7681 10505 652
Size: 7029 2824 3640
Fragments arranged by size:
7029 3640 2824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 1684 2082 2324 2401 2557 2977 3159 3417
Size: 875 398 242 77 156 420 182 258
Cuts at: 3417 3556 3618 3679 4043 4054 4143 4640 5118
Size: 139 62 61 364 11 89 497 478
Cuts at: 5118 5143 5582 6098 6387 6874 6961 8112 8207
Size: 25 439 516 289 487 87 1151 95
Cuts at: 8207 8378 8791 8803 9155 9345 10309 11318 11750
Size: 171 413 12 352 190 964 1009 432
Cuts at: 11750 12497 12765 809
Size: 747 268 1537
Fragments arranged by size:
1537 1151 1009 964 875 747 516 497
487 478 439 432 420 413 398 364
352 289 268 258 242 190 182 171
156 139 95 89 87 77 62 61
25 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 1054 2483 4801 1054
Size: 1429 2318 9746
Fragments arranged by size:
9746 2318 1429
EcoO109I rG'GnC_Cy
Cuts at: 2343 3468 5327 7457 8665 10632 2343
Size: 1125 1859 2130 1208 1967 5204
Fragments arranged by size:
5204 2130 1967 1859 1208 1125
EcoRI G'AATT_C
Cuts at: 2615 8864 10497 2615
Size: 6249 1633 5611
Fragments arranged by size:
6249 5611 1633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3025 3316 4670 8808 11512 3025
Size: 291 1354 4138 2704 5006
Fragments arranged by size:
5006 4138 2704 1354 291
FspI TGC'GCA
Cuts at: 6739 11274 6739
Size: 4535 8958
HaeII r_GCGC'y
Cuts at: 151 1905 8138 8160 10927 11175 12197 13176 151
Size: 1754 6233 22 2767 248 1022 979 468
Fragments arranged by size:
6233 2767 1754 1022 979 468 248 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 431 463 712 744 1258 1290 1351 1383 1388
Size: 32 249 32 514 32 61 32 5
Cuts at: 1388 1420 1943 1975 2200 2232 4534 4566 4581
Size: 32 523 32 225 32 2302 32 15
Cuts at: 4581 4613 4747 4779 4990 5022 5571 5603 5867
Size: 32 134 32 211 32 549 32 264
Cuts at: 5867 5899 6193 6225 7778 7810 7998 8030 8751
Size: 32 294 32 1553 32 188 32 721
Cuts at: 8751 8783 9248 9280 9535 9567 9688 9720 9865
Size: 32 465 32 255 32 121 32 145
Cuts at: 9865 9897 11335 11365 11367 11397 431
Size: 32 1438 30 2 30 2527
Fragments arranged by size:
2527 2302 1553 1438 721 549 523 514
465 294 264 255 249 225 211 188
145 134 121 61 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 15 5 2
HincII GTy'rAC
Cuts at: 6838 9657 10203 10532 6838
Size: 2819 546 329 9799
Fragments arranged by size:
9799 2819 546 329
HindIII A'AGCT_T
Cuts at: 2247 8583 2247
Size: 6336 7157
KasI G'GCGC_C
Cuts at: 10923 11171 10923
Size: 248 13245
KpnI G_GTAC'C
Cuts at: 3270 6432 10513 3270
Size: 3162 4081 6250
Fragments arranged by size:
6250 4081 3162
MfeI C'AATT_G
Cuts at: 2099 6012 9277 9770 2099
Size: 3913 3265 493 5822
Fragments arranged by size:
5822 3913 3265 493
MluI A'CGCG_T
Cuts at: 12926 12926
Size: 13493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 679 3829 4497 4733 5242 6601 7675 8370 8443
Size: 3150 668 236 509 1359 1074 695 73
Cuts at: 8443 8566 9017 12164 12348 679
Size: 123 451 3147 184 1824
Fragments arranged by size:
3150 3147 1824 1359 1074 695 668 509
451 236 184 123 73
MscI TGG'CCA
Cuts at: 2332 2792 2918 4361 7568 9393 11254 2332
Size: 460 126 1443 3207 1825 1861 4571
Fragments arranged by size:
4571 3207 1861 1825 1443 460 126
MslI CAynn'nnrTG
Cuts at: 818 955 1292 1580 3062 3313 4833 6404 6745
Size: 137 337 288 1482 251 1520 1571 341
Cuts at: 6745 7135 7555 7769 8206 11482 11609 818
Size: 390 420 214 437 3276 127 2702
Fragments arranged by size:
3276 2702 1571 1520 1482 437 420 390
341 337 288 251 214 137 127
MspA1I CmG'CkG
Cuts at: 3137 3456 3670 3701 6214 8024 9690 9728 9815
Size: 319 214 31 2513 1810 1666 38 87
Cuts at: 9815 9999 10139 10604 10918 11278 12291 12536 3137
Size: 184 140 465 314 360 1013 245 4094
Fragments arranged by size:
4094 2513 1810 1666 1013 465 360 319
314 245 214 184 140 87 38 31
NaeI GCC'GGC
Cuts at: 6450 8175 11674 6450
Size: 1725 3499 8269
Fragments arranged by size:
8269 3499 1725
NarI GG'CG_CC
Cuts at: 10924 11172 10924
Size: 248 13245
NcoI C'CATG_G
Cuts at: 107 1287 1502 1896 4362 7569 8694 10014 107
Size: 1180 215 394 2466 3207 1125 1320 3586
Fragments arranged by size:
3586 3207 2466 1320 1180 1125 394 215
NdeI CA'TA_TG
Cuts at: 843 3247 843
Size: 2404 11089
NgoMIV G'CCGG_C
Cuts at: 6448 8173 11672 6448
Size: 1725 3499 8269
Fragments arranged by size:
8269 3499 1725
NheI G'CTAG_C
Cuts at: 3609 3609
Size: 13493
NsiI A_TGCA'T
Cuts at: 815 7582 815
Size: 6767 6726
NspI r_CATG'y
Cuts at: 2094 3216 6744 6775 7560 8205 8637 10125 10225
Size: 1122 3528 31 785 645 432 1488 100
Cuts at: 10225 10546 11046 11953 2094
Size: 321 500 907 3634
Fragments arranged by size:
3634 3528 1488 1122 907 785 645 500
432 321 100 31
PasI CC'CwG_GG
Cuts at: 4570 7022 4570
Size: 2452 11041
PciI A'CATG_T
Cuts at: 2090 3212 7556 8201 11949 2090
Size: 1122 4344 645 3748 3634
Fragments arranged by size:
4344 3748 3634 1122 645
PflMI CCAn_nnn'nTGG
Cuts at: 2083 2788 5224 9084 9282 10170 2083
Size: 705 2436 3860 198 888 5406
Fragments arranged by size:
5406 3860 2436 888 705 198
PfoI T'CCnGG_A
Cuts at: 315 2534 4274 4769 8730 315
Size: 2219 1740 495 3961 5078
Fragments arranged by size:
5078 3961 2219 1740 495
PmeI GTTT'AAAC
Cuts at: 10492 10492
Size: 13493
PmlI CAC'GTG
Cuts at: 504 2459 9780 504
Size: 1955 7321 4217
Fragments arranged by size:
7321 4217 1955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12632 12664 12632
Size: 32 13461
PpuMI rG'GwC_Cy
Cuts at: 3468 5327 7457 3468
Size: 1859 2130 9504
Fragments arranged by size:
9504 2130 1859
PshAI GACnn'nnGTC
Cuts at: 10467 10467
Size: 13493
PsiI TTA'TAA
Cuts at: 5897 5897
Size: 13493
PspOMI G'GGCC_C
Cuts at: 125 5998 8664 125
Size: 5873 2666 4954
Fragments arranged by size:
5873 4954 2666
PspXI vC'TCGA_Gb
Cuts at: 10082 10082
Size: 13493
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 9431 9463 9431
Size: 32 13461
PstI C_TGCA'G
Cuts at: 4137 4357 8169 8689 9814 10540 11225 4137
Size: 220 3812 520 1125 726 685 6405
Fragments arranged by size:
6405 3812 1125 726 685 520 220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 13493
PvuII CAG'CTG
Cuts at: 3456 3670 9690 9999 10139 10918 11278 3456
Size: 214 6020 309 140 779 360 5671
Fragments arranged by size:
6020 5671 779 360 309 214 140
RsrII CG'GwC_CG
Cuts at: 11688 11688
Size: 13493
SacI G_AGCT'C
Cuts at: 654 7683 10507 654
Size: 7029 2824 3640
Fragments arranged by size:
7029 3640 2824
SacII CC_GC'GG
Cuts at: 6215 6215
Size: 13493
SalI G'TCGA_C
Cuts at: 10530 10530
Size: 13493
SapI GCTCTTCn'nnn_
Cuts at: 11516 11726 11516
Size: 210 13283
SbfI CC_TGCA'GG
Cuts at: 10540 10540
Size: 13493
ScaI AGT'ACT
Cuts at: 2962 4053 4075 8626 8847 13010 2962
Size: 1091 22 4551 221 4163 3445
Fragments arranged by size:
4551 4163 3445 1091 221 22
SexAI A'CCwGG_T
Cuts at: 2592 2945 3691 7453 2592
Size: 353 746 3762 8632
Fragments arranged by size:
8632 3762 746 353
SfcI C'TryA_G
Cuts at: 33 584 1241 1391 1769 1992 4009 4133 4353
Size: 551 657 150 378 223 2017 124 220
Cuts at: 4353 4946 5121 6850 7014 7240 8165 8685 9810
Size: 593 175 1729 164 226 925 520 1125
Cuts at: 9810 10536 11221 12214 12405 33
Size: 726 685 993 191 1121
Fragments arranged by size:
2017 1729 1125 1121 993 925 726 685
657 593 551 520 378 226 223 220
191 175 164 150 124
SfoI GGC'GCC
Cuts at: 10925 11173 10925
Size: 248 13245
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 13493
SmaI CCC'GGG
Cuts at: 10515 10515
Size: 13493
SmlI C'TyrA_G
Cuts at: 2835 3161 3977 8706 9663 10082 10648 11563 12055
Size: 326 816 4729 957 419 566 915 492
Cuts at: 12055 12317 2835
Size: 262 4011
Fragments arranged by size:
4729 4011 957 915 816 566 492 419
326 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 13493
SphI G_CATG'C
Cuts at: 10546 11046 10546
Size: 500 12993
SspI AAT'ATT
Cuts at: 7056 7900 7056
Size: 844 12649
StuI AGG'CCT
Cuts at: 872 872
Size: 13493
StyI C'CwwG_G
Cuts at: 107 1287 1502 1896 2082 2766 3270 4234 4362
Size: 1180 215 394 186 684 504 964 128
Cuts at: 4362 4977 5413 7569 7881 8694 9867 10014 10627
Size: 615 436 2156 312 813 1173 147 613
Cuts at: 10627 10981 107
Size: 354 2619
Fragments arranged by size:
2619 2156 1180 1173 964 813 684 615
613 504 436 394 354 312 215 186
147 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 244 689 1988 3302 3474 6031 6481 7136 7346
Size: 445 1299 1314 172 2557 450 655 210
Cuts at: 7346 7841 11076 11209 11668 11877 244
Size: 495 3235 133 459 209 1860
Fragments arranged by size:
3235 2557 1860 1314 1299 655 495 459
450 445 210 209 172 133
TatI w'GTAC_w
Cuts at: 2960 3428 3575 3620 4051 4073 4325 5151 5394
Size: 468 147 45 431 22 252 826 243
Cuts at: 5394 5674 7622 7726 7955 8257 8624 8845 9782
Size: 280 1948 104 229 302 367 221 937
Cuts at: 9782 10028 13008 2960
Size: 246 2980 3445
Fragments arranged by size:
3445 2980 1948 937 826 468 431 367
302 280 252 246 243 229 221 147
104 45 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4412 4444 5304 5336 5441 5473 6585 6617 8143
Size: 32 860 32 105 32 1112 32 1526
Cuts at: 8143 8175 8309 8341 8384 8416 8891 8923 13010
Size: 32 134 32 43 32 475 32 4087
Cuts at: 13010 13042 4412
Size: 32 4863
Fragments arranged by size:
4863 4087 1526 1112 860 475 134 105
43 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3493 8077 9700 11290 3493
Size: 4584 1623 1590 5696
Fragments arranged by size:
5696 4584 1623 1590
XbaI T'CTAG_A
Cuts at: 5667 10524 5667
Size: 4857 8636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 907 4519 5358 8549 9279 9784 10988 907
Size: 3612 839 3191 730 505 1204 3412
Fragments arranged by size:
3612 3412 3191 1204 839 730 505
XhoI C'TCGA_G
Cuts at: 3977 10082 3977
Size: 6105 7388
XmaI C'CCGG_G
Cuts at: 10513 10513
Size: 13493
XmnI GAAnn'nnTTC
Cuts at: 1103 1352 3008 6696 7056 8517 8776 1103
Size: 249 1656 3688 360 1461 259 5820
Fragments arranged by size:
5820 3688 1656 1461 360 259 249
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI HpaI NotI NruI PacI SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI