Restriction map for
pF1KB4563
(Circular) (Six-base) MAPSORT of: pF1KB4563.seq Check: 2967 from: 1 to: 5843
pF1KB4563 5843 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2460 2460
Size: 5843
AccI GT'mk_AC
Cuts at: 640 2881 640
Size: 2241 3602
Acc65I G'GTAC_C
Cuts at: 931 2342 2440 2859 931
Size: 1411 98 419 3915
Fragments arranged by size:
3915 1411 419 98
AclI AA'CG_TT
Cuts at: 5683 5683
Size: 5843
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 994 1654 1695 1709 1821 2442 3668 4100
Size: 132 660 41 14 112 621 1226 432
Cuts at: 4100 4453 5115 862
Size: 353 662 1590
Fragments arranged by size:
1590 1226 662 660 621 432 353 132
112 41 14
AflIII A'CryG_T
Cuts at: 1301 2385 3195 4995 5276 1301
Size: 1084 810 1800 281 1868
Fragments arranged by size:
1868 1800 1084 810 281
AhdI GACnn_n'nnGTC
Cuts at: 151 151
Size: 5843
AleI CACnn'nnGTG
Cuts at: 2467 2606 2467
Size: 139 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1154 1600 2485 1154
Size: 446 885 4512
Fragments arranged by size:
4512 885 446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 2701
Size: 32 5811
AlwNI CAG_nnn'CTG
Cuts at: 1158 1405 1659 2586 3148 4586 1158
Size: 247 254 927 562 1438 2415
Fragments arranged by size:
2415 1438 927 562 254 247
ApaI G_GGCC'C
Cuts at: 738 2085 738
Size: 1347 4496
ApaLI G'TGCA_C
Cuts at: 4681 4681
Size: 5843
ApoI r'AATT_y
Cuts at: 853 1318 2323 2821 2847 5196 5452 853
Size: 465 1005 498 26 2349 256 1244
Fragments arranged by size:
2349 1244 1005 498 465 256 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5843
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
AvaI C'yCGr_G
Cuts at: 353 1022 1313 2351 2465 2863 353
Size: 669 291 1038 114 398 3333
Fragments arranged by size:
3333 1038 669 398 291 114
BamHI G'GATC_C
Cuts at: 2868 2868
Size: 5843
BanI G'GyrC_C
Cuts at: 931 1951 2218 2342 2440 2514 2859 3273 3521
Size: 1020 267 124 98 74 345 414 248
Cuts at: 3521 3556 931
Size: 35 3218
Fragments arranged by size:
3218 1020 414 345 267 248 124 98
74 35
BanII G_rGCy'C
Cuts at: 338 738 968 1800 1835 2085 2287 2857 3887
Size: 400 230 832 35 250 202 570 1030
Cuts at: 3887 338
Size: 2294
Fragments arranged by size:
2294 1030 832 570 400 250 230 202
35
BbeI G_GCGC'C
Cuts at: 3277 3525 3277
Size: 248 5595
BbsI GAAGACnn'nnnn_
Cuts at: 1701 2382 1701
Size: 681 5162
BbvCI CC'TCA_GC
Cuts at: 259 1039 1460 259
Size: 780 421 4642
Fragments arranged by size:
4642 780 421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1911 1945 2356 2390 1911
Size: 34 411 34 5364
Fragments arranged by size:
5364 411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 315 1629 3741 4797 5843 315
Size: 1314 2112 1056 1046 315
Fragments arranged by size:
2112 1314 1056 1046 315
BclI T'GATC_A
Cuts at: 1493 2729 3363 1493
Size: 1236 634 3973
Fragments arranged by size:
3973 1236 634
BglI GCCn_nnn'nGGC
Cuts at: 2050 2050
Size: 5843
BglII A'GATC_T
Cuts at: 3358 5395 3358
Size: 2037 3806
BlpI GC'TnA_GC
Cuts at: 501 2955 501
Size: 2454 3389
Bme1580I G_kGCm'C
Cuts at: 738 793 2085 2352 2519 3468 3561 4685 738
Size: 55 1292 267 167 949 93 1124 1896
Fragments arranged by size:
1896 1292 1124 949 267 167 93 55
BmrI ACTGGGnnnn_n'
Cuts at: 2217 3055 3136 3471 5615 2217
Size: 838 81 335 2144 2445
Fragments arranged by size:
2445 2144 838 335 81
BmtI G_CTAG'C
Cuts at: 2150 2150
Size: 5843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4210 4210
Size: 5843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 922 1261 2146 812
Size: 110 339 885 4509
Fragments arranged by size:
4509 885 339 110
Bpu10I CC'TnA_GC
Cuts at: 242 259 539 1039 1460 242
Size: 17 280 500 421 4625
Fragments arranged by size:
4625 500 421 280 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 2465 3019 3898 4612 4910 619
Size: 1846 554 879 714 298 1552
Fragments arranged by size:
1846 1552 879 714 554 298
BsaI GGTCTCn'nnnn_
Cuts at: 2206 5547 2206
Size: 3341 2502
BsaAI yAC'GTr
Cuts at: 2199 3196 2199
Size: 997 4846
BsaBI GATnn'nnATC
Cuts at: 6 3357 5394 6
Size: 3351 2037 455
Fragments arranged by size:
3351 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 2543 3274 3522 4224 5720 112
Size: 2431 731 248 702 1496 235
Fragments arranged by size:
2431 1496 731 702 248 235
BsaWI w'CCGG_w
Cuts at: 3032 3553 4231 4642 4789 5063 3032
Size: 521 678 411 147 274 3812
Fragments arranged by size:
3812 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1972 2002 2701 2731 1972
Size: 30 699 30 5084
Fragments arranged by size:
5084 699 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 227 733 958 1808 2062 2107 2122 2143
Size: 101 506 225 850 254 45 15 21
Cuts at: 2143 126
Size: 3826
Fragments arranged by size:
3826 850 506 254 225 101 45 21
15
BseYI C'CCAG_C
Cuts at: 1386 2664 3268 4026 4691 5099 1386
Size: 1278 604 758 665 408 2130
Fragments arranged by size:
2130 1278 758 665 604 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1840 1924 2173 2221 184
Size: 1656 84 249 48 3806
Fragments arranged by size:
3806 1656 249 84 48
BsiEI CG_ry'CG
Cuts at: 104 3385 3431 4661 104
Size: 3281 46 1230 1286
Fragments arranged by size:
3281 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 968 1982 2857 3635 3825 4685 968
Size: 1014 875 778 190 860 2126
Fragments arranged by size:
2126 1014 875 860 778 190
BsmI GAATG_Cn'
Cuts at: 1167 1789 2751 1167
Size: 622 962 4259
Fragments arranged by size:
4259 962 622
BsmBI CGTCTCn'nnnn_
Cuts at: 1729 1729
Size: 5843
Bsp1286I G_dGCh'C
Cuts at: 338 738 793 968 1800 1835 1982 2085 2287
Size: 400 55 175 832 35 147 103 202
Cuts at: 2287 2352 2519 2857 3468 3561 3635 3825 3887
Size: 65 167 338 611 93 74 190 62
Cuts at: 3887 4685 338
Size: 798 1496
Fragments arranged by size:
1496 832 798 611 400 338 202 190
175 167 147 103 93 74 65 62
55 35
BspHI T'CATG_A
Cuts at: 4275 4275
Size: 5843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1143 1669 2195 2460 2893 3107 3409 3790 1143
Size: 526 526 265 433 214 302 381 3196
Fragments arranged by size:
3196 526 526 433 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 128 246 4135 4189 5673 5838 50
Size: 78 118 3889 54 1484 165 55
Fragments arranged by size:
3889 1484 165 118 78 55 54
BsrDI GCAATG_nn'
Cuts at: 473 1454 2258 2259 2315 3755 473
Size: 981 804 1 56 1440 2561
Fragments arranged by size:
2561 1440 981 804 56 1
BsrFI r'CCGG_y
Cuts at: 548 1502 1918 2215 2611 3841 4022 548
Size: 954 416 297 396 1230 181 2369
Fragments arranged by size:
2369 1230 954 416 396 297 181
BssSI C'ACGA_G
Cuts at: 224 4114 4822 224
Size: 3890 708 1245
Fragments arranged by size:
3890 1245 708
BstAPI GCAn_nnn'nTGC
Cuts at: 788 1659 1784 3220 788
Size: 871 125 1436 3411
Fragments arranged by size:
3411 1436 871 125
BstBI TT'CG_AA
Cuts at: 366 1326 4204 366
Size: 960 2878 2005
Fragments arranged by size:
2878 2005 960
BstXI CCAn_nnnn'nTGG
Cuts at: 1869 2436 1869
Size: 567 5276
BstYI r'GATC_y
Cuts at: 1 630 2868 3335 3358 3693 3939 4245 4257
Size: 629 2238 467 23 335 246 306 12
Cuts at: 4257 4343 5395 1
Size: 86 1052 449
Fragments arranged by size:
2238 1052 629 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 641 641
Size: 5843
BtgI C'CryG_G
Cuts at: 107 149 2102 2486 2546 107
Size: 42 1953 384 60 3404
Fragments arranged by size:
3404 1953 384 60 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2669 3793 3974 4137 2669
Size: 1124 181 163 4375
Fragments arranged by size:
4375 1124 181 163
BtsI GCAGTG_nn'
Cuts at: 729 825 3086 729
Size: 96 2261 3486
Fragments arranged by size:
3486 2261 96
DraI TTT'AAA
Cuts at: 2842 2842
Size: 5843
DraIII CAC_nnn'GTG
Cuts at: 666 994 2582 666
Size: 328 1588 3927
Fragments arranged by size:
3927 1588 328
DrdI GACnn_nn'nnGTC
Cuts at: 3549 4893 3549
Size: 1344 4499
EaeI y'GGCC_r
Cuts at: 3428 3602 3993 4020 5365 3428
Size: 174 391 27 1345 3906
Fragments arranged by size:
3906 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3428 3428
Size: 5843
EarI CTCTTCn'nnn_
Cuts at: 3866 4076 3866
Size: 210 5633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4051 4793 4939 5653 4051
Size: 742 146 714 4241
Fragments arranged by size:
4241 742 714 146
EcoICRI GAG'CTC
Cuts at: 966 2855 966
Size: 1889 3954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 862 922 994 1261 1654 1695 1709 1821
Size: 50 60 72 267 393 41 14 112
Cuts at: 1821 2146 2442 3668 4100 4453 5115 812
Size: 325 296 1226 432 353 662 1540
Fragments arranged by size:
1540 1226 662 432 393 353 325 296
267 112 72 60 50 41 14
EcoNI CCTnn'n_nnAGG
Cuts at: 973 973
Size: 5843
EcoO109I rG'GnC_Cy
Cuts at: 145 734 735 2418 2982 145
Size: 589 1 1683 564 3006
Fragments arranged by size:
3006 1683 589 564 1
EcoRI G'AATT_C
Cuts at: 2847 2847
Size: 5843
EcoRV GAT'ATC
Cuts at: 2568 2568
Size: 5843
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1870 2016 3862 1870
Size: 146 1846 3851
Fragments arranged by size:
3851 1846 146
FspI TGC'GCA
Cuts at: 2251 2704 3624 2251
Size: 453 920 4470
Fragments arranged by size:
4470 920 453
HaeII r_GCGC'y
Cuts at: 2771 2790 3277 3525 4755 5526 2771
Size: 19 487 248 1230 771 3088
Fragments arranged by size:
3088 1230 771 487 248 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 1507 1539 3685 3715 3717 3747 137
Size: 32 1338 32 2146 30 2 30 2233
Fragments arranged by size:
2233 2146 1338 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2882 2882
Size: 5843
HindIII A'AGCT_T
Cuts at: 1333 1333
Size: 5843
KasI G'GCGC_C
Cuts at: 3273 3521 3273
Size: 248 5595
KpnI G_GTAC'C
Cuts at: 935 2346 2444 2863 935
Size: 1411 98 419 3915
Fragments arranged by size:
3915 1411 419 98
MluI A'CGCG_T
Cuts at: 5276 5276
Size: 5843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1251 2207 4602 4786 1251
Size: 956 2395 184 2308
Fragments arranged by size:
2395 2308 956 184
MscI TGG'CCA
Cuts at: 3604 3604
Size: 5843
MslI CAynn'nnrTG
Cuts at: 181 947 1177 1535 2467 2512 2606 3832 3959
Size: 766 230 358 932 45 94 1226 127
Cuts at: 3959 181
Size: 2065
Fragments arranged by size:
2065 1226 932 766 358 230 127 94
45
MspA1I CmG'CkG
Cuts at: 151 168 1674 2954 3268 3628 4412 4657 151
Size: 17 1506 1280 314 360 784 245 1337
Fragments arranged by size:
1506 1337 1280 784 360 314 245 17
NaeI GCC'GGC
Cuts at: 1920 2613 4024 1920
Size: 693 1411 3739
Fragments arranged by size:
3739 1411 693
NarI GG'CG_CC
Cuts at: 3274 3522 3274
Size: 248 5595
NcoI C'CATG_G
Cuts at: 107 107
Size: 5843
NgoMIV G'CCGG_C
Cuts at: 1918 2611 4022 1918
Size: 693 1411 3739
Fragments arranged by size:
3739 1411 693
NheI G'CTAG_C
Cuts at: 2146 2146
Size: 5843
NruI TCG'CGA
Cuts at: 870 870
Size: 5843
NspI r_CATG'y
Cuts at: 712 1305 2250 2389 2896 3396 4999 712
Size: 593 945 139 507 500 1603 1556
Fragments arranged by size:
1603 1556 945 593 507 500 139
PasI CC'CwG_GG
Cuts at: 338 420 338
Size: 82 5761
PciI A'CATG_T
Cuts at: 1301 2385 4995 1301
Size: 1084 2610 2149
Fragments arranged by size:
2610 2149 1084
PfoI T'CCnGG_A
Cuts at: 626 626
Size: 5843
PmeI GTTT'AAAC
Cuts at: 2842 2842
Size: 5843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2733 4289 4321 2701
Size: 32 1556 32 4223
Fragments arranged by size:
4223 1556 32 32
PpuMI rG'GwC_Cy
Cuts at: 145 2418 145
Size: 2273 3570
PspOMI G'GGCC_C
Cuts at: 734 2081 734
Size: 1347 4496
PstI C_TGCA'G
Cuts at: 846 1554 1880 2636 2890 3575 846
Size: 708 326 756 254 685 3114
Fragments arranged by size:
3114 756 708 685 326 254
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5843
PvuII CAG'CTG
Cuts at: 3268 3628 3268
Size: 360 5483
RsrII CG'GwC_CG
Cuts at: 4038 4038
Size: 5843
SacI G_AGCT'C
Cuts at: 968 2857 968
Size: 1889 3954
SacII CC_GC'GG
Cuts at: 152 152
Size: 5843
SalI G'TCGA_C
Cuts at: 2880 2880
Size: 5843
SanDI GG'GwC_CC
Cuts at: 145 145
Size: 5843
SapI GCTCTTCn'nnn_
Cuts at: 3866 4076 3866
Size: 210 5633
SbfI CC_TGCA'GG
Cuts at: 2890 2890
Size: 5843
ScaI AGT'ACT
Cuts at: 5360 5360
Size: 5843
SexAI A'CCwGG_T
Cuts at: 758 758
Size: 5843
SfcI C'TryA_G
Cuts at: 33 842 1550 1876 2276 2632 2829 2886 3571
Size: 809 708 326 400 356 197 57 685
Cuts at: 3571 4539 4730 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 809 708 685 400 356 326
197 191 57
SfoI GGC'GCC
Cuts at: 3275 3523 3275
Size: 248 5595
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5843
SmaI CCC'GGG
Cuts at: 1024 1315 2865 1024
Size: 291 1550 4002
Fragments arranged by size:
4002 1550 291
SmlI C'TyrA_G
Cuts at: 353 634 2480 2998 3913 4627 4889 353
Size: 281 1846 518 915 714 262 1307
Fragments arranged by size:
1846 1307 915 714 518 281 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5843
SphI G_CATG'C
Cuts at: 2896 3396 2896
Size: 500 5343
StyI C'CwwG_G
Cuts at: 107 447 655 1142 1862 1991 2648 2977 3331
Size: 340 208 487 720 129 657 329 354
Cuts at: 3331 107
Size: 2619
Fragments arranged by size:
2619 720 657 487 354 340 329 208
129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1270 2185 2236 3426 3559 4018 4227 1270
Size: 915 51 1190 133 459 209 2886
Fragments arranged by size:
2886 1190 915 459 209 133 51
TatI w'GTAC_w
Cuts at: 5358 5358
Size: 5843
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 608 617 640 1972 2004 5360 5392 585
Size: 23 9 23 1332 32 3356 32 1036
Fragments arranged by size:
3356 1332 1036 32 32 23 23 9
Tth111I GACn'n_nGTC
Cuts at: 3640 3640
Size: 5843
XbaI T'CTAG_A
Cuts at: 2874 2874
Size: 5843
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1110 2655 3338 1110
Size: 1545 683 3615
Fragments arranged by size:
3615 1545 683
XhoI C'TCGA_G
Cuts at: 353 353
Size: 5843
XmaI C'CCGG_G
Cuts at: 1022 1313 2863 1022
Size: 291 1550 4002
Fragments arranged by size:
4002 1550 291
XmnI GAAnn'nnTTC
Cuts at: 1322 2716 1322
Size: 1394 4449
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AscI AvrII BaeI BfrBI
BmgBI BsiWI BspEI BsrGI BssHII BstEII Bsu36I ClaI
CspCI FseI FspAI HpaI MfeI NdeI NotI NsiI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI