Restriction map for
pF1KB4567
(Circular) (Six-base) MAPSORT of: pF1KB4567.seq Check: 9 from: 1 to: 5393
pF1KB4567 5393 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1272 1323 1272
Size: 51 5342
AccI GT'mk_AC
Cuts at: 867 2243 2431 867
Size: 1376 188 3829
Fragments arranged by size:
3829 1376 188
Acc65I G'GTAC_C
Cuts at: 2409 2409
Size: 5393
AclI AA'CG_TT
Cuts at: 5233 5233
Size: 5393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 446 530 1805 1855 1882 2279 3218 3650
Size: 183 84 1275 50 27 397 939 432
Cuts at: 3650 4397 4665 263
Size: 747 268 991
Fragments arranged by size:
1275 991 939 747 432 397 268 183
84 50 27
AflII C'TTAA_G
Cuts at: 697 697
Size: 5393
AflIII A'CryG_T
Cuts at: 2745 3849 4826 2745
Size: 1104 977 3312
Fragments arranged by size:
3312 1104 977
AgeI A'CCGG_T
Cuts at: 3781 3781
Size: 5393
AlwNI CAG_nnn'CTG
Cuts at: 1045 2698 4265 1045
Size: 1653 1567 2173
Fragments arranged by size:
2173 1653 1567
ApaI G_GGCC'C
Cuts at: 750 750
Size: 5393
ApaLI G'TGCA_C
Cuts at: 4163 4163
Size: 5393
ApoI r'AATT_y
Cuts at: 1004 2263 2397 4746 5002 1004
Size: 1259 134 2349 256 1395
Fragments arranged by size:
2349 1395 1259 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 5393
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
AvaI C'yCGr_G
Cuts at: 2413 2413
Size: 5393
BamHI G'GATC_C
Cuts at: 2418 2418
Size: 5393
BanI G'GyrC_C
Cuts at: 1260 2409 2823 3071 3106 1260
Size: 1149 414 248 35 3547
Fragments arranged by size:
3547 1149 414 248 35
BanII G_rGCy'C
Cuts at: 750 1924 2330 2407 3437 750
Size: 1174 406 77 1030 2706
Fragments arranged by size:
2706 1174 1030 406 77
BbeI G_GCGC'C
Cuts at: 2827 3075 2827
Size: 248 5145
BbsI GAAGACnn'nnnn_
Cuts at: 1300 1673 1793 1300
Size: 373 120 4900
Fragments arranged by size:
4900 373 120
BbvCI CC'TCA_GC
Cuts at: 1693 1693
Size: 5393
BciVI GTATCCnnnnn_n'
Cuts at: 3291 4052 5393 3291
Size: 761 1341 3291
Fragments arranged by size:
3291 1341 761
BclI T'GATC_A
Cuts at: 2913 2913
Size: 5393
BfrBI ATG'CAT
Cuts at: 1399 1399
Size: 5393
BglI GCCn_nnn'nGGC
Cuts at: 1776 1776
Size: 5393
BglII A'GATC_T
Cuts at: 487 776 1830 2908 4945 487
Size: 289 1054 1078 2037 935
Fragments arranged by size:
2037 1078 1054 935 289
BlpI GC'TnA_GC
Cuts at: 2505 2505
Size: 5393
Bme1580I G_kGCm'C
Cuts at: 750 1037 1484 3018 3111 4167 750
Size: 287 447 1534 93 1056 1976
Fragments arranged by size:
1976 1534 1056 447 287 93
BmrI ACTGGGnnnn_n'
Cuts at: 2605 2686 3021 5165 2605
Size: 81 335 2144 2833
Fragments arranged by size:
2833 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 5393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 1197 1977 470
Size: 727 780 3886
Fragments arranged by size:
3886 780 727
Bpu10I CC'TnA_GC
Cuts at: 1607 1693 1607
Size: 86 5307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2569 3448 3940 4238 2569
Size: 879 492 298 3724
Fragments arranged by size:
3724 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1267 1533 1561 5097 1267
Size: 266 28 3536 1563
Fragments arranged by size:
3536 1563 266 28
BsaAI yAC'GTr
Cuts at: 2746 2746
Size: 5393
BsaBI GATnn'nnATC
Cuts at: 6 1823 1829 2907 4944 6
Size: 1817 6 1078 2037 455
Fragments arranged by size:
2037 1817 1078 455 6
BsaHI Gr'CG_yC
Cuts at: 2824 3072 3774 5270 2824
Size: 248 702 1496 2947
Fragments arranged by size:
2947 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2582 3103 3781 4055 4202 4613 2582
Size: 521 678 274 147 411 3362
Fragments arranged by size:
3362 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2303 2333 2303
Size: 30 5363
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1223 1638 1223
Size: 415 4978
BseYI C'CCAG_C
Cuts at: 290 348 1547 2818 3576 4153 4649 290
Size: 58 1199 1271 758 577 496 1034
Fragments arranged by size:
1271 1199 1034 758 577 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2180
Size: 5393
BsiEI CG_ry'CG
Cuts at: 104 2935 2981 4189 104
Size: 2831 46 1208 1308
Fragments arranged by size:
2831 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2330 2407 3185 3375 4167 2330
Size: 77 778 190 792 3556
Fragments arranged by size:
3556 792 778 190 77
BsmBI CGTCTCn'nnnn_
Cuts at: 1321 1321
Size: 5393
Bsp1286I G_dGCh'C
Cuts at: 750 1037 1484 1924 2330 2407 3018 3111 3185
Size: 287 447 440 406 77 611 93 74
Cuts at: 3185 3375 3437 4167 750
Size: 190 62 730 1976
Fragments arranged by size:
1976 730 611 447 440 406 287 190
93 77 74 62
BspEI T'CCGG_A
Cuts at: 4613 4613
Size: 5393
BspHI T'CATG_A
Cuts at: 4569 4569
Size: 5393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1272 1285 1323 2443 2657 2959 3340 1272
Size: 13 38 1120 214 302 381 3325
Fragments arranged by size:
3325 1120 381 302 214 38 13
BsrBI CCG'CTC
Cuts at: 50 3685 3739 5223 5388 50
Size: 3635 54 1484 165 55
Fragments arranged by size:
3635 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 142 1250 3305 142
Size: 1108 2055 2230
Fragments arranged by size:
2230 2055 1108
BsrFI r'CCGG_y
Cuts at: 3391 3572 3781 3391
Size: 181 209 5003
Fragments arranged by size:
5003 209 181
BssSI C'ACGA_G
Cuts at: 3664 4022 3664
Size: 358 5035
BstAPI GCAn_nnn'nTGC
Cuts at: 2770 2770
Size: 5393
BstBI TT'CG_AA
Cuts at: 3754 3754
Size: 5393
BstEII G'GTnAC_C
Cuts at: 500 500
Size: 5393
BstYI r'GATC_y
Cuts at: 1 487 555 776 1497 1830 2418 2885 2908
Size: 486 68 221 721 333 588 467 23
Cuts at: 2908 3243 3489 4501 4587 4599 4945 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 721 588 486 467 449 346 335
333 246 221 86 68 23 12
Bsu36I CC'TnA_GG
Cuts at: 1046 1814 1046
Size: 768 4625
BtgI C'CryG_G
Cuts at: 107 107
Size: 5393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3343 3524 3687 3343
Size: 181 163 5049
Fragments arranged by size:
5049 181 163
BtsI GCAGTG_nn'
Cuts at: 2636 2636
Size: 5393
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1704 1739 1704
Size: 35 5358
DraI TTT'AAA
Cuts at: 1449 2392 1449
Size: 943 4450
DrdI GACnn_nn'nnGTC
Cuts at: 3099 3957 3099
Size: 858 4535
EaeI y'GGCC_r
Cuts at: 920 1322 1705 2978 3152 3543 3570 4915 920
Size: 402 383 1273 174 391 27 1345 1398
Fragments arranged by size:
1398 1345 1273 402 391 383 174 27
EagI C'GGCC_G
Cuts at: 2978 2978
Size: 5393
EarI CTCTTCn'nnn_
Cuts at: 259 1520 1973 1989 2095 2378 3416 3626 259
Size: 1261 453 16 106 283 1038 210 2026
Fragments arranged by size:
2026 1261 1038 453 283 210 106 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3601 3911 4057 5203 3601
Size: 310 146 1146 3791
Fragments arranged by size:
3791 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2328 2405 2328
Size: 77 5316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 446 470 530 1197 1805 1855 1882 1977
Size: 183 24 60 667 608 50 27 95
Cuts at: 1977 2279 3218 3650 4397 4665 263
Size: 302 939 432 747 268 991
Fragments arranged by size:
991 939 747 667 608 432 302 268
183 95 60 50 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 520 520
Size: 5393
EcoO109I rG'GnC_Cy
Cuts at: 308 1042 2532 308
Size: 734 1490 3169
Fragments arranged by size:
3169 1490 734
EcoRI G'AATT_C
Cuts at: 2263 2397 2263
Size: 134 5259
EcoRV GAT'ATC
Cuts at: 532 532
Size: 5393
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1449 1868 3412 1449
Size: 419 1544 3430
Fragments arranged by size:
3430 1544 419
FspI TGC'GCA
Cuts at: 3174 3174
Size: 5393
HaeII r_GCGC'y
Cuts at: 2827 3075 4097 5076 2827
Size: 248 1022 979 3144
Fragments arranged by size:
3144 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 479 511 1287 1319 2303 2335 3235 3265 3267
Size: 32 776 32 984 32 900 30 2
Cuts at: 3267 3297 479
Size: 30 2575
Fragments arranged by size:
2575 984 900 776 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 186 2432 186
Size: 2246 3147
KasI G'GCGC_C
Cuts at: 2823 3071 2823
Size: 248 5145
KpnI G_GTAC'C
Cuts at: 2413 2413
Size: 5393
MluI A'CGCG_T
Cuts at: 4826 4826
Size: 5393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4064 4248 4064
Size: 184 5209
MscI TGG'CCA
Cuts at: 922 1324 1707 3154 922
Size: 402 383 1447 3161
Fragments arranged by size:
3161 1447 402 383
MslI CAynn'nnrTG
Cuts at: 3382 3509 3382
Size: 127 5266
MspA1I CmG'CkG
Cuts at: 241 687 937 1349 1678 2504 2818 3178 4191
Size: 446 250 412 329 826 314 360 1013
Cuts at: 4191 4436 241
Size: 245 1198
Fragments arranged by size:
1198 1013 826 446 412 360 329 314
250 245
NaeI GCC'GGC
Cuts at: 3574 3574
Size: 5393
NarI GG'CG_CC
Cuts at: 2824 3072 2824
Size: 248 5145
NcoI C'CATG_G
Cuts at: 107 107
Size: 5393
NdeI CA'TA_TG
Cuts at: 1395 1395
Size: 5393
NgoMIV G'CCGG_C
Cuts at: 3572 3572
Size: 5393
NsiI A_TGCA'T
Cuts at: 1401 1401
Size: 5393
NspI r_CATG'y
Cuts at: 1965 2446 2946 3853 1965
Size: 481 500 907 3505
Fragments arranged by size:
3505 907 500 481
PasI CC'CwG_GG
Cuts at: 343 1582 343
Size: 1239 4154
PciI A'CATG_T
Cuts at: 3849 3849
Size: 5393
PflMI CCAn_nnn'nTGG
Cuts at: 2295 2295
Size: 5393
PmeI GTTT'AAAC
Cuts at: 2392 2392
Size: 5393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4532 4564 4532
Size: 32 5361
PpuMI rG'GwC_Cy
Cuts at: 308 1042 308
Size: 734 4659
PshAI GACnn'nnGTC
Cuts at: 498 498
Size: 5393
PspOMI G'GGCC_C
Cuts at: 746 746
Size: 5393
PstI C_TGCA'G
Cuts at: 2440 3125 2440
Size: 685 4708
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5393
PvuII CAG'CTG
Cuts at: 241 937 1349 1678 2818 3178 241
Size: 696 412 329 1140 360 2456
Fragments arranged by size:
2456 1140 696 412 360 329
RsrII CG'GwC_CG
Cuts at: 3588 3588
Size: 5393
SacI G_AGCT'C
Cuts at: 2330 2407 2330
Size: 77 5316
SalI G'TCGA_C
Cuts at: 2430 2430
Size: 5393
SanDI GG'GwC_CC
Cuts at: 308 308
Size: 5393
SapI GCTCTTCn'nnn_
Cuts at: 3416 3626 3416
Size: 210 5183
SbfI CC_TGCA'GG
Cuts at: 2440 2440
Size: 5393
ScaI AGT'ACT
Cuts at: 4910 4910
Size: 5393
SfcI C'TryA_G
Cuts at: 33 1886 2436 3121 4114 4305 33
Size: 1853 550 685 993 191 1121
Fragments arranged by size:
1853 1121 993 685 550 191
SfoI GGC'GCC
Cuts at: 2825 3073 2825
Size: 248 5145
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5393
SmaI CCC'GGG
Cuts at: 2415 2415
Size: 5393
SmlI C'TyrA_G
Cuts at: 697 2548 3463 3955 4217 697
Size: 1851 915 492 262 1873
Fragments arranged by size:
1873 1851 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5393
SphI G_CATG'C
Cuts at: 2446 2946 2446
Size: 500 4893
SspI AAT'ATT
Cuts at: 218 218
Size: 5393
StuI AGG'CCT
Cuts at: 1645 1645
Size: 5393
StyI C'CwwG_G
Cuts at: 107 1640 1742 2027 2527 2881 107
Size: 1533 102 285 500 354 2619
Fragments arranged by size:
2619 1533 500 354 285 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1075 1603 1978 2976 3109 3568 3777 1075
Size: 528 375 998 133 459 209 2691
Fragments arranged by size:
2691 998 528 459 375 209 133
TatI w'GTAC_w
Cuts at: 4908 4908
Size: 5393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4910 4942 4910
Size: 32 5361
Tth111I GACn'n_nGTC
Cuts at: 3190 3190
Size: 5393
XbaI T'CTAG_A
Cuts at: 490 798 1827 2424 490
Size: 308 1029 597 3459
Fragments arranged by size:
3459 1029 597 308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 445 2888 445
Size: 2443 2950
XmaI C'CCGG_G
Cuts at: 2413 2413
Size: 5393
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I ClaI DraIII FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PfoI PmlI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI