Restriction map for
pF1KB4571
(Circular) (Six-base) MAPSORT of: pF1KB4571.seq Check: 590 from: 1 to: 4958
pF1KB4571 4958 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 372 890 1996 372
Size: 518 1106 3334
Fragments arranged by size:
3334 1106 518
Acc65I G'GTAC_C
Cuts at: 1974 1974
Size: 4958
AclI AA'CG_TT
Cuts at: 4798 4798
Size: 4958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1072 2783 3215 3568 4230 493
Size: 579 1711 432 353 662 1221
Fragments arranged by size:
1711 1221 662 579 432 353
AflIII A'CryG_T
Cuts at: 1070 2310 4110 4391 1070
Size: 1240 1800 281 1637
Fragments arranged by size:
1800 1637 1240 281
AlwNI CAG_nnn'CTG
Cuts at: 2263 3701 2263
Size: 1438 3520
ApaLI G'TGCA_C
Cuts at: 3796 3796
Size: 4958
ApoI r'AATT_y
Cuts at: 121 185 562 831 1620 1962 4311 4567 121
Size: 64 377 269 789 342 2349 256 512
Fragments arranged by size:
2349 789 512 377 342 269 256 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4958
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
AvaI C'yCGr_G
Cuts at: 1978 1978
Size: 4958
BamHI G'GATC_C
Cuts at: 1983 1983
Size: 4958
BanI G'GyrC_C
Cuts at: 1974 2388 2636 2671 1974
Size: 414 248 35 4261
Fragments arranged by size:
4261 414 248 35
BanII G_rGCy'C
Cuts at: 1972 3002 1972
Size: 1030 3928
BbeI G_GCGC'C
Cuts at: 2392 2640 2392
Size: 248 4710
BbsI GAAGACnn'nnnn_
Cuts at: 817 984 817
Size: 167 4791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4924
BciVI GTATCCnnnnn_n'
Cuts at: 2856 3912 4958 2856
Size: 1056 1046 2856
Fragments arranged by size:
2856 1056 1046
BclI T'GATC_A
Cuts at: 2478 2478
Size: 4958
BfrBI ATG'CAT
Cuts at: 1636 1636
Size: 4958
BglII A'GATC_T
Cuts at: 2473 4510 2473
Size: 2037 2921
BlpI GC'TnA_GC
Cuts at: 2070 2070
Size: 4958
Bme1580I G_kGCm'C
Cuts at: 390 1928 2583 2676 3800 390
Size: 1538 655 93 1124 1548
Fragments arranged by size:
1548 1538 1124 655 93
BmrI ACTGGGnnnn_n'
Cuts at: 815 1646 2170 2251 2586 4730 815
Size: 831 524 81 335 2144 1043
Fragments arranged by size:
2144 1043 831 524 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 4958
Bpu10I CC'TnA_GC
Cuts at: 515 515
Size: 4958
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1146 2134 3013 3727 4025 967
Size: 179 988 879 714 298 1900
Fragments arranged by size:
1900 988 879 714 298 179
BsaI GGTCTCn'nnnn_
Cuts at: 4662 4662
Size: 4958
BsaAI yAC'GTr
Cuts at: 1071 2311 1071
Size: 1240 3718
BsaBI GATnn'nnATC
Cuts at: 6 2472 4509 6
Size: 2466 2037 455
Fragments arranged by size:
2466 2037 455
BsaHI Gr'CG_yC
Cuts at: 330 2389 2637 3339 4835 330
Size: 2059 248 702 1496 453
Fragments arranged by size:
2059 1496 702 453 248
BsaWI w'CCGG_w
Cuts at: 1640 2147 2668 3346 3757 3904 4178 1640
Size: 507 521 678 411 147 274 2420
Fragments arranged by size:
2420 678 521 507 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 1475 1505 190
Size: 30 1255 30 3643
Fragments arranged by size:
3643 1255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 752 830 752
Size: 78 4880
BseYI C'CCAG_C
Cuts at: 209 2383 3141 3806 4214 209
Size: 2174 758 665 408 953
Fragments arranged by size:
2174 953 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 372 2500 2546 3776 104
Size: 268 2128 46 1230 1286
Fragments arranged by size:
2128 1286 1230 268 46
BsiHKAI G_wGCw'C
Cuts at: 1972 2750 2940 3800 1972
Size: 778 190 860 3130
Fragments arranged by size:
3130 860 778 190
BsmI GAATG_Cn'
Cuts at: 1636 1636
Size: 4958
Bsp1286I G_dGCh'C
Cuts at: 390 1928 1972 2583 2676 2750 2940 3002 3800
Size: 1538 44 611 93 74 190 62 798
Cuts at: 3800 390
Size: 1548
Fragments arranged by size:
1548 1538 798 611 190 93 74 62
44
BspEI T'CCGG_A
Cuts at: 1640 1640
Size: 4958
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2008 2222 2524 2905 2008
Size: 214 302 381 4061
Fragments arranged by size:
4061 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3250 3304 4788 4953 50
Size: 3200 54 1484 165 55
Fragments arranged by size:
3200 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2870 2870
Size: 4958
BsrFI r'CCGG_y
Cuts at: 2956 3137 2956
Size: 181 4777
BsrGI T'GTAC_A
Cuts at: 1073 1073
Size: 4958
BssSI C'ACGA_G
Cuts at: 1862 3229 3937 1862
Size: 1367 708 2883
Fragments arranged by size:
2883 1367 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2335 2335
Size: 4958
BstBI TT'CG_AA
Cuts at: 3319 3319
Size: 4958
BstXI CCAn_nnnn'nTGG
Cuts at: 1752 1752
Size: 4958
BstYI r'GATC_y
Cuts at: 1 816 1983 2450 2473 2808 3054 3360 3372
Size: 815 1167 467 23 335 246 306 12
Cuts at: 3372 3458 4510 1
Size: 86 1052 449
Fragments arranged by size:
1167 1052 815 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1439 1439
Size: 4958
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2908 3089 3252 2908
Size: 181 163 4614
Fragments arranged by size:
4614 181 163
BtsI GCAGTG_nn'
Cuts at: 692 2201 692
Size: 1509 3449
DraI TTT'AAA
Cuts at: 1676 1957 1676
Size: 281 4677
DrdI GACnn_nn'nnGTC
Cuts at: 2664 4008 2664
Size: 1344 3614
EaeI y'GGCC_r
Cuts at: 1103 1218 1361 1558 2543 2717 3108 3135 4480
Size: 115 143 197 985 174 391 27 1345
Cuts at: 4480 1103
Size: 1581
Fragments arranged by size:
1581 1345 985 391 197 174 143 115
27
EagI C'GGCC_G
Cuts at: 2543 2543
Size: 4958
EarI CTCTTCn'nnn_
Cuts at: 615 790 1051 2981 3191 615
Size: 175 261 1930 210 2382
Fragments arranged by size:
2382 1930 261 210 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3166 3908 4054 4768 3166
Size: 742 146 714 3356
Fragments arranged by size:
3356 742 714 146
EcoICRI GAG'CTC
Cuts at: 1970 1970
Size: 4958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 1072 2783 3215 3568 4230 493
Size: 579 1711 432 353 662 1221
Fragments arranged by size:
1711 1221 662 579 432 353
EcoO109I rG'GnC_Cy
Cuts at: 2097 2097
Size: 4958
EcoRI G'AATT_C
Cuts at: 1962 1962
Size: 4958
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4958
FspI TGC'GCA
Cuts at: 2739 2739
Size: 4958
HaeII r_GCGC'y
Cuts at: 1162 2392 2640 3870 4641 1162
Size: 1230 248 1230 771 1479
Fragments arranged by size:
1479 1230 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 614 646 1119 1151 2800 2830 2832
Size: 32 392 32 473 32 1649 30 2
Cuts at: 2832 2862 190
Size: 30 2286
Fragments arranged by size:
2286 1649 473 392 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 373 1433 1997 373
Size: 1060 564 3334
Fragments arranged by size:
3334 1060 564
HindIII A'AGCT_T
Cuts at: 1663 1663
Size: 4958
KasI G'GCGC_C
Cuts at: 2388 2636 2388
Size: 248 4710
KpnI G_GTAC'C
Cuts at: 1978 1978
Size: 4958
MfeI C'AATT_G
Cuts at: 935 935
Size: 4958
MluI A'CGCG_T
Cuts at: 4391 4391
Size: 4958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3717 3901 3717
Size: 184 4774
MscI TGG'CCA
Cuts at: 1363 1560 2719 1363
Size: 197 1159 3602
Fragments arranged by size:
3602 1159 197
MslI CAynn'nnrTG
Cuts at: 1544 2947 3074 1544
Size: 1403 127 3428
Fragments arranged by size:
3428 1403 127
MspA1I CmG'CkG
Cuts at: 465 2069 2383 2743 3527 3772 465
Size: 1604 314 360 784 245 1651
Fragments arranged by size:
1651 1604 784 360 314 245
NaeI GCC'GGC
Cuts at: 3139 3139
Size: 4958
NarI GG'CG_CC
Cuts at: 2389 2637 2389
Size: 248 4710
NcoI C'CATG_G
Cuts at: 1439 1439
Size: 4958
NgoMIV G'CCGG_C
Cuts at: 3137 3137
Size: 4958
NsiI A_TGCA'T
Cuts at: 1638 1638
Size: 4958
NspI r_CATG'y
Cuts at: 1146 2011 2511 4114 1146
Size: 865 500 1603 1990
Fragments arranged by size:
1990 1603 865 500
PciI A'CATG_T
Cuts at: 4110 4110
Size: 4958
PmeI GTTT'AAAC
Cuts at: 1957 1957
Size: 4958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4926
PstI C_TGCA'G
Cuts at: 1029 2005 2690 1029
Size: 976 685 3297
Fragments arranged by size:
3297 976 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4958
PvuII CAG'CTG
Cuts at: 2383 2743 2383
Size: 360 4598
RsrII CG'GwC_CG
Cuts at: 3153 3153
Size: 4958
SacI G_AGCT'C
Cuts at: 1972 1972
Size: 4958
SalI G'TCGA_C
Cuts at: 371 1995 371
Size: 1624 3334
SapI GCTCTTCn'nnn_
Cuts at: 2981 3191 2981
Size: 210 4748
SbfI CC_TGCA'GG
Cuts at: 2005 2005
Size: 4958
ScaI AGT'ACT
Cuts at: 1912 4475 1912
Size: 2563 2395
SexAI A'CCwGG_T
Cuts at: 744 744
Size: 4958
SfcI C'TryA_G
Cuts at: 33 1025 2001 2686 3654 3845 33
Size: 992 976 685 968 191 1146
Fragments arranged by size:
1146 992 976 968 685 191
SfoI GGC'GCC
Cuts at: 2390 2638 2390
Size: 248 4710
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4958
SmaI CCC'GGG
Cuts at: 1980 1980
Size: 4958
SmlI C'TyrA_G
Cuts at: 982 1125 2113 3028 3742 4004 982
Size: 143 988 915 714 262 1936
Fragments arranged by size:
1936 988 915 714 262 143
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4958
SphI G_CATG'C
Cuts at: 2011 2511 2011
Size: 500 4458
StyI C'CwwG_G
Cuts at: 1358 1439 1772 2092 2446 1358
Size: 81 333 320 354 3870
Fragments arranged by size:
3870 354 333 320 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 1744 2541 2674 3133 3342 1284
Size: 460 797 133 459 209 2900
Fragments arranged by size:
2900 797 460 459 209 133
TatI w'GTAC_w
Cuts at: 1073 1910 4473 1073
Size: 837 2563 1558
Fragments arranged by size:
2563 1558 837
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1475 1507 4475 4507 1475
Size: 32 2968 32 1926
Fragments arranged by size:
2968 1926 32 32
Tth111I GACn'n_nGTC
Cuts at: 2755 2755
Size: 4958
XbaI T'CTAG_A
Cuts at: 1989 1989
Size: 4958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1459 2453 1459
Size: 994 3964
XmaI C'CCGG_G
Cuts at: 1978 1978
Size: 4958
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BglI BmgBI
BmtI BpmI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI