Restriction map for
pF1KB4573
(Circular) (Six-base) MAPSORT of: pF1KB4573.seq Check: 813 from: 1 to: 5057
pF1KB4573 5057 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1412 1412
Size: 5057
AccI GT'mk_AC
Cuts at: 877 1247 1700 2095 877
Size: 370 453 395 3839
Fragments arranged by size:
3839 453 395 370
Acc65I G'GTAC_C
Cuts at: 2073 2073
Size: 5057
AclI AA'CG_TT
Cuts at: 4897 4897
Size: 5057
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 869 962 1260 2882 3314 3667 4329 568
Size: 301 93 298 1622 432 353 662 1296
Fragments arranged by size:
1622 1296 662 432 353 301 298 93
AfeI AGC'GCT
Cuts at: 1965 1965
Size: 5057
AflIII A'CryG_T
Cuts at: 2409 4209 4490 2409
Size: 1800 281 2976
Fragments arranged by size:
2976 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 749 749
Size: 5057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 931 931
Size: 5057
AlwNI CAG_nnn'CTG
Cuts at: 1072 1914 2362 3800 1072
Size: 842 448 1438 2329
Fragments arranged by size:
2329 1438 842 448
ApaLI G'TGCA_C
Cuts at: 3895 3895
Size: 5057
ApoI r'AATT_y
Cuts at: 577 619 699 2061 4410 4666 577
Size: 42 80 1362 2349 256 968
Fragments arranged by size:
2349 1362 968 256 80 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 5057
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
AvaI C'yCGr_G
Cuts at: 2034 2077 2034
Size: 43 5014
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 629 662 629
Size: 33 5024
BamHI G'GATC_C
Cuts at: 2082 2082
Size: 5057
BanI G'GyrC_C
Cuts at: 1062 2073 2487 2735 2770 1062
Size: 1011 414 248 35 3349
Fragments arranged by size:
3349 1011 414 248 35
BanII G_rGCy'C
Cuts at: 1012 2071 3101 1012
Size: 1059 1030 2968
Fragments arranged by size:
2968 1059 1030
BbeI G_GCGC'C
Cuts at: 2491 2739 2491
Size: 248 4809
BbsI GAAGACnn'nnnn_
Cuts at: 139 544 1910 2040 139
Size: 405 1366 130 3156
Fragments arranged by size:
3156 1366 405 130
BciVI GTATCCnnnnn_n'
Cuts at: 2955 4011 5057 2955
Size: 1056 1046 2955
Fragments arranged by size:
2955 1056 1046
BclI T'GATC_A
Cuts at: 2577 2577
Size: 5057
BglI GCCn_nnn'nGGC
Cuts at: 1016 1016
Size: 5057
BglII A'GATC_T
Cuts at: 1812 2572 4609 1812
Size: 760 2037 2260
Fragments arranged by size:
2260 2037 760
BlpI GC'TnA_GC
Cuts at: 2169 2169
Size: 5057
Bme1580I G_kGCm'C
Cuts at: 1065 2682 2775 3899 1065
Size: 1617 93 1124 2223
Fragments arranged by size:
2223 1617 1124 93
BmgBI CAC'GTC
Cuts at: 2030 2030
Size: 5057
BmrI ACTGGGnnnn_n'
Cuts at: 1365 2269 2350 2685 4829 1365
Size: 904 81 335 2144 1593
Fragments arranged by size:
2144 1593 904 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1619 3424 1619
Size: 1805 3252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 687 819 1614 165
Size: 522 132 795 3608
Fragments arranged by size:
3608 795 522 132
Bpu10I CC'TnA_GC
Cuts at: 1466 1466
Size: 5057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 2233 3112 3826 4124 349
Size: 1884 879 714 298 1282
Fragments arranged by size:
1884 1282 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1411 4761 1411
Size: 3350 1707
BsaAI yAC'GTr
Cuts at: 2410 2410
Size: 5057
BsaBI GATnn'nnATC
Cuts at: 6 2571 4608 6
Size: 2565 2037 455
Fragments arranged by size:
2565 2037 455
BsaHI Gr'CG_yC
Cuts at: 2488 2736 3438 4934 2488
Size: 248 702 1496 2611
Fragments arranged by size:
2611 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1958 2246 2767 3445 3856 4003 4277 1958
Size: 288 521 678 411 147 274 2738
Fragments arranged by size:
2738 678 521 411 288 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1408 1438 1408
Size: 30 5027
BseYI C'CCAG_C
Cuts at: 726 2482 3240 3905 4313 726
Size: 1756 758 665 408 1470
Fragments arranged by size:
1756 1470 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 416
Size: 5057
BsiEI CG_ry'CG
Cuts at: 104 2599 2645 3875 104
Size: 2495 46 1230 1286
Fragments arranged by size:
2495 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 116 632 2071 2849 3039 3899 116
Size: 516 1439 778 190 860 1274
Fragments arranged by size:
1439 1274 860 778 516 190
BsmI GAATG_Cn'
Cuts at: 1667 1667
Size: 5057
Bsp1286I G_dGCh'C
Cuts at: 116 632 1012 1065 2071 2682 2775 2849 3039
Size: 516 380 53 1006 611 93 74 190
Cuts at: 3039 3101 3899 116
Size: 62 798 1274
Fragments arranged by size:
1274 1006 798 611 516 380 190 93
74 62 53
BspHI T'CATG_A
Cuts at: 624 3489 624
Size: 2865 2192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1412 1599 2107 2321 2623 3004 1412
Size: 187 508 214 302 381 3465
Fragments arranged by size:
3465 508 381 302 214 187
BsrBI CCG'CTC
Cuts at: 50 1059 3349 3403 4887 5052 50
Size: 1009 2290 54 1484 165 55
Fragments arranged by size:
2290 1484 1009 165 55 54
BsrDI GCAATG_nn'
Cuts at: 150 1448 1853 2969 150
Size: 1298 405 1116 2238
Fragments arranged by size:
2238 1298 1116 405
BsrFI r'CCGG_y
Cuts at: 3055 3236 3055
Size: 181 4876
BssSI C'ACGA_G
Cuts at: 1103 3328 4036 1103
Size: 2225 708 2124
Fragments arranged by size:
2225 2124 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2434 2434
Size: 5057
BstBI TT'CG_AA
Cuts at: 951 3418 951
Size: 2467 2590
BstXI CCAn_nnnn'nTGG
Cuts at: 1019 1019
Size: 5057
BstYI r'GATC_y
Cuts at: 1 255 1232 1812 1910 2082 2549 2572 2907
Size: 254 977 580 98 172 467 23 335
Cuts at: 2907 3153 3459 3471 3557 4609 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 977 580 467 449 335 306 254
246 172 98 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5057
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3007 3188 3351 3007
Size: 181 163 4713
Fragments arranged by size:
4713 181 163
BtsI GCAGTG_nn'
Cuts at: 404 2300 404
Size: 1896 3161
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1152 1187 1152
Size: 35 5022
DraI TTT'AAA
Cuts at: 2056 2056
Size: 5057
DrdI GACnn_nn'nnGTC
Cuts at: 2763 4107 2763
Size: 1344 3713
EaeI y'GGCC_r
Cuts at: 2642 2816 3207 3234 4579 2642
Size: 174 391 27 1345 3120
Fragments arranged by size:
3120 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2642 2642
Size: 5057
EarI CTCTTCn'nnn_
Cuts at: 1846 1883 3080 3290 1846
Size: 37 1197 210 3613
Fragments arranged by size:
3613 1197 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3265 4007 4153 4867 3265
Size: 742 146 714 3455
Fragments arranged by size:
3455 742 714 146
EcoICRI GAG'CTC
Cuts at: 2069 2069
Size: 5057
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 568 687 819 869 962 1260 1614 2882
Size: 403 119 132 50 93 298 354 1268
Cuts at: 2882 3314 3667 4329 165
Size: 432 353 662 893
Fragments arranged by size:
1268 893 662 432 403 354 353 298
132 119 93 50
EcoO109I rG'GnC_Cy
Cuts at: 375 1076 2196 375
Size: 701 1120 3236
Fragments arranged by size:
3236 1120 701
EcoRI G'AATT_C
Cuts at: 2061 2061
Size: 5057
EcoRV GAT'ATC
Cuts at: 2007 2007
Size: 5057
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 573 3076 573
Size: 2503 2554
FspI TGC'GCA
Cuts at: 1300 2838 1300
Size: 1538 3519
HaeII r_GCGC'y
Cuts at: 228 1967 2491 2739 3969 4740 228
Size: 1739 524 248 1230 771 545
Fragments arranged by size:
1739 1230 771 545 524 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 735 767 1501 1533 1687 1719 1760
Size: 32 255 32 734 32 154 32 41
Cuts at: 1760 1792 2899 2929 2931 2961 448
Size: 32 1107 30 2 30 2544
Fragments arranged by size:
2544 1107 734 255 154 41 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 878 1248 2096 878
Size: 370 848 3839
Fragments arranged by size:
3839 848 370
HindIII A'AGCT_T
Cuts at: 550 550
Size: 5057
KasI G'GCGC_C
Cuts at: 2487 2735 2487
Size: 248 4809
KpnI G_GTAC'C
Cuts at: 2077 2077
Size: 5057
MfeI C'AATT_G
Cuts at: 1361 1361
Size: 5057
MluI A'CGCG_T
Cuts at: 4490 4490
Size: 5057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1673 1959 3816 4000 1544
Size: 129 286 1857 184 2601
Fragments arranged by size:
2601 1857 286 184 129
MscI TGG'CCA
Cuts at: 2818 2818
Size: 5057
MslI CAynn'nnrTG
Cuts at: 1217 1406 1783 3046 3173 1217
Size: 189 377 1263 127 3101
Fragments arranged by size:
3101 1263 377 189 127
MspA1I CmG'CkG
Cuts at: 185 2168 2482 2842 3626 3871 185
Size: 1983 314 360 784 245 1371
Fragments arranged by size:
1983 1371 784 360 314 245
NaeI GCC'GGC
Cuts at: 3238 3238
Size: 5057
NarI GG'CG_CC
Cuts at: 2488 2736 2488
Size: 248 4809
NcoI C'CATG_G
Cuts at: 107 107
Size: 5057
NgoMIV G'CCGG_C
Cuts at: 3236 3236
Size: 5057
NspI r_CATG'y
Cuts at: 1315 1411 1489 2110 2610 4213 1315
Size: 96 78 621 500 1603 2159
Fragments arranged by size:
2159 1603 621 500 96 78
PciI A'CATG_T
Cuts at: 4209 4209
Size: 5057
PflMI CCAn_nnn'nTGG
Cuts at: 1072 1072
Size: 5057
PfoI T'CCnGG_A
Cuts at: 497 772 1412 497
Size: 275 640 4142
Fragments arranged by size:
4142 640 275
PmeI GTTT'AAAC
Cuts at: 2056 2056
Size: 5057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 5025
PpuMI rG'GwC_Cy
Cuts at: 1076 1076
Size: 5057
PshAI GACnn'nnGTC
Cuts at: 569 569
Size: 5057
PsiI TTA'TAA
Cuts at: 1199 1199
Size: 5057
PstI C_TGCA'G
Cuts at: 2104 2789 2104
Size: 685 4372
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5057
PvuII CAG'CTG
Cuts at: 185 2482 2842 185
Size: 2297 360 2400
Fragments arranged by size:
2400 2297 360
RsrII CG'GwC_CG
Cuts at: 3252 3252
Size: 5057
SacI G_AGCT'C
Cuts at: 2071 2071
Size: 5057
SalI G'TCGA_C
Cuts at: 876 1246 2094 876
Size: 370 848 3839
Fragments arranged by size:
3839 848 370
SanDI GG'GwC_CC
Cuts at: 1076 1076
Size: 5057
SapI GCTCTTCn'nnn_
Cuts at: 3080 3290 3080
Size: 210 4847
SbfI CC_TGCA'GG
Cuts at: 2104 2104
Size: 5057
ScaI AGT'ACT
Cuts at: 4574 4574
Size: 5057
SfcI C'TryA_G
Cuts at: 33 633 2100 2785 3753 3944 33
Size: 600 1467 685 968 191 1146
Fragments arranged by size:
1467 1146 968 685 600 191
SfoI GGC'GCC
Cuts at: 2489 2737 2489
Size: 248 4809
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
SmaI CCC'GGG
Cuts at: 2079 2079
Size: 5057
SmlI C'TyrA_G
Cuts at: 364 2212 3127 3841 4103 364
Size: 1848 915 714 262 1318
Fragments arranged by size:
1848 1318 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5057
SphI G_CATG'C
Cuts at: 1489 2110 2610 1489
Size: 621 500 3936
Fragments arranged by size:
3936 621 500
StuI AGG'CCT
Cuts at: 1101 1273 1896 1101
Size: 172 623 4262
Fragments arranged by size:
4262 623 172
StyI C'CwwG_G
Cuts at: 107 2191 2545 107
Size: 2084 354 2619
Fragments arranged by size:
2619 2084 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2640 2773 3232 3441 2640
Size: 133 459 209 4256
Fragments arranged by size:
4256 459 209 133
TatI w'GTAC_w
Cuts at: 4572 4572
Size: 5057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 822 854 878 910 4574 4606 822
Size: 32 24 32 3664 32 1273
Fragments arranged by size:
3664 1273 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 2028 2854 2028
Size: 826 4231
XbaI T'CTAG_A
Cuts at: 2088 2088
Size: 5057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 918 2552 918
Size: 1634 3423
XmaI C'CCGG_G
Cuts at: 2077 2077
Size: 5057
XmnI GAAnn'nnTTC
Cuts at: 482 541 482
Size: 59 4998
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BmtI BseRI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI