Restriction map for
pF1KB4575
(Circular) (Six-base) MAPSORT of: pF1KB4575.seq Check: 8703 from: 1 to: 5768
pF1KB4575 5768 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2806 2806
Size: 5768
Acc65I G'GTAC_C
Cuts at: 1416 2031 2784 1416
Size: 615 753 4400
Fragments arranged by size:
4400 753 615
AclI AA'CG_TT
Cuts at: 534 1176 1333 5608 534
Size: 642 157 4275 694
Fragments arranged by size:
4275 694 642 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 566 840 1283 1450 1771 3593 4025 4378
Size: 319 274 443 167 321 1822 432 353
Cuts at: 4378 5040 247
Size: 662 975
Fragments arranged by size:
1822 975 662 443 432 353 321 319
274 167
AfeI AGC'GCT
Cuts at: 2677 2677
Size: 5768
AflIII A'CryG_T
Cuts at: 648 3120 4920 5201 648
Size: 2472 1800 281 1215
Fragments arranged by size:
2472 1800 1215 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1517 1517
Size: 5768
AlwNI CAG_nnn'CTG
Cuts at: 1855 3073 4511 1855
Size: 1218 1438 3112
Fragments arranged by size:
3112 1438 1218
ApaLI G'TGCA_C
Cuts at: 2002 4606 2002
Size: 2604 3164
ApoI r'AATT_y
Cuts at: 1013 1215 1358 1566 2772 5121 5377 1013
Size: 202 143 208 1206 2349 256 1404
Fragments arranged by size:
2349 1404 1206 256 208 202 143
AseI AT'TA_AT
Cuts at: 20 20
Size: 5768
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
AvaI C'yCGr_G
Cuts at: 279 2788 279
Size: 2509 3259
BamHI G'GATC_C
Cuts at: 2793 2793
Size: 5768
BanI G'GyrC_C
Cuts at: 1416 2031 2784 3198 3446 3481 1416
Size: 615 753 414 248 35 3703
Fragments arranged by size:
3703 753 615 414 248 35
BanII G_rGCy'C
Cuts at: 436 1702 2782 3812 436
Size: 1266 1080 1030 2392
Fragments arranged by size:
2392 1266 1080 1030
BbeI G_GCGC'C
Cuts at: 3202 3450 3202
Size: 248 5520
BbsI GAAGACnn'nnnn_
Cuts at: 2651 2651
Size: 5768
BciVI GTATCCnnnnn_n'
Cuts at: 3666 4722 5768 3666
Size: 1056 1046 3666
Fragments arranged by size:
3666 1056 1046
BclI T'GATC_A
Cuts at: 3288 3288
Size: 5768
BglII A'GATC_T
Cuts at: 3283 5320 3283
Size: 2037 3731
BlpI GC'TnA_GC
Cuts at: 2672 2880 2672
Size: 208 5560
Bme1580I G_kGCm'C
Cuts at: 597 1093 2006 3393 3486 4610 597
Size: 496 913 1387 93 1124 1755
Fragments arranged by size:
1755 1387 1124 913 496 93
BmrI ACTGGGnnnn_n'
Cuts at: 2980 3061 3396 5540 2980
Size: 81 335 2144 3208
Fragments arranged by size:
3208 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1697 4135 1697
Size: 2438 3330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1025 1510 1982 2651 1025
Size: 485 472 669 4142
Fragments arranged by size:
4142 669 485 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 2700 2944 3823 4537 4835 420
Size: 2280 244 879 714 298 1353
Fragments arranged by size:
2280 1353 879 714 298 244
BsaI GGTCTCn'nnnn_
Cuts at: 5472 5472
Size: 5768
BsaAI yAC'GTr
Cuts at: 3121 3121
Size: 5768
BsaBI GATnn'nnATC
Cuts at: 6 717 2372 3282 5319 6
Size: 711 1655 910 2037 455
Fragments arranged by size:
2037 1655 910 711 455
BsaHI Gr'CG_yC
Cuts at: 2274 3199 3447 4149 5645 2274
Size: 925 248 702 1496 2397
Fragments arranged by size:
2397 1496 925 702 248
BsaWI w'CCGG_w
Cuts at: 2957 3478 4156 4567 4714 4988 2957
Size: 521 678 411 147 274 3737
Fragments arranged by size:
3737 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1894 1924 1894
Size: 30 5738
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 444 835 2586 2632 267
Size: 177 391 1751 46 3403
Fragments arranged by size:
3403 1751 391 177 46
BseYI C'CCAG_C
Cuts at: 900 3193 3951 4616 5024 900
Size: 2293 758 665 408 1644
Fragments arranged by size:
2293 1644 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1692 1874 1692
Size: 182 5586
BsiEI CG_ry'CG
Cuts at: 104 3310 3356 4586 104
Size: 3206 46 1230 1286
Fragments arranged by size:
3206 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 436 891 1683 1702 2006 2072 2115 2782 3560
Size: 455 792 19 304 66 43 667 778
Cuts at: 3560 3750 4610 436
Size: 190 860 1594
Fragments arranged by size:
1594 860 792 778 667 455 304 190
66 43 19
BsmI GAATG_Cn'
Cuts at: 1506 1506
Size: 5768
Bsp1286I G_dGCh'C
Cuts at: 436 597 891 1093 1683 1702 2006 2072 2115
Size: 161 294 202 590 19 304 66 43
Cuts at: 2115 2782 3393 3486 3560 3750 3812 4610 436
Size: 667 611 93 74 190 62 798 1594
Fragments arranged by size:
1594 798 667 611 590 304 294 202
190 161 93 74 66 62 43 19
BspHI T'CATG_A
Cuts at: 1881 2277 2493 4200 1881
Size: 396 216 1707 3449
Fragments arranged by size:
3449 1707 396 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2818 3032 3334 3715 2818
Size: 214 302 381 4871
Fragments arranged by size:
4871 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4060 4114 5598 5763 50
Size: 4010 54 1484 165 55
Fragments arranged by size:
4010 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1476 3680 1476
Size: 2204 3564
BsrFI r'CCGG_y
Cuts at: 3766 3947 3766
Size: 181 5587
BssSI C'ACGA_G
Cuts at: 4039 4747 4039
Size: 708 5060
BstAPI GCAn_nnn'nTGC
Cuts at: 3145 3145
Size: 5768
BstBI TT'CG_AA
Cuts at: 4129 4129
Size: 5768
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 5768
BstYI r'GATC_y
Cuts at: 1 2793 3260 3283 3618 3864 4170 4182 4268
Size: 2792 467 23 335 246 306 12 86
Cuts at: 4268 5320 1
Size: 1052 449
Fragments arranged by size:
2792 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2624 3718 3899 4062 2624
Size: 1094 181 163 4330
Fragments arranged by size:
4330 1094 181 163
BtsI GCAGTG_nn'
Cuts at: 1812 2520 3011 1812
Size: 708 491 4569
Fragments arranged by size:
4569 708 491
DraI TTT'AAA
Cuts at: 1104 1181 2767 1104
Size: 77 1586 4105
Fragments arranged by size:
4105 1586 77
DrdI GACnn_nn'nnGTC
Cuts at: 859 3474 4818 859
Size: 2615 1344 1809
Fragments arranged by size:
2615 1809 1344
EaeI y'GGCC_r
Cuts at: 3353 3527 3918 3945 5290 3353
Size: 174 391 27 1345 3831
Fragments arranged by size:
3831 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3353 3353
Size: 5768
EarI CTCTTCn'nnn_
Cuts at: 2191 2270 2331 2604 2609 2666 2727 3791 4001
Size: 79 61 273 5 57 61 1064 210
Cuts at: 4001 2191
Size: 3958
Fragments arranged by size:
3958 1064 273 210 79 61 61 57
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 315 3976 4718 4864 5578 315
Size: 3661 742 146 714 505
Fragments arranged by size:
3661 742 714 505 146
EcoICRI GAG'CTC
Cuts at: 434 1700 2780 434
Size: 1266 1080 3422
Fragments arranged by size:
3422 1266 1080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 566 840 1025 1283 1450 1510 1771 1982
Size: 319 274 185 258 167 60 261 211
Cuts at: 1982 2651 3593 4025 4378 5040 247
Size: 669 942 432 353 662 975
Fragments arranged by size:
975 942 669 662 432 353 319 274
261 258 211 185 167 60
EcoNI CCTnn'n_nnAGG
Cuts at: 497 1673 497
Size: 1176 4592
EcoO109I rG'GnC_Cy
Cuts at: 904 2047 2907 904
Size: 1143 860 3765
Fragments arranged by size:
3765 1143 860
EcoRI G'AATT_C
Cuts at: 1358 2772 1358
Size: 1414 4354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 3787 1978
Size: 1809 3959
FspI TGC'GCA
Cuts at: 3549 3549
Size: 5768
HaeII r_GCGC'y
Cuts at: 419 2679 3202 3450 4680 5451 419
Size: 2260 523 248 1230 771 736
Fragments arranged by size:
2260 1230 771 736 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 1213 1245 2578 2581 2610 2613 3610
Size: 32 940 32 1333 3 29 3 997
Cuts at: 3610 3640 3642 3672 241
Size: 30 2 30 2337
Fragments arranged by size:
2337 1333 997 940 32 32 30 30
29 3 3 2
HincII GTy'rAC
Cuts at: 956 1337 2807 956
Size: 381 1470 3917
Fragments arranged by size:
3917 1470 381
HindIII A'AGCT_T
Cuts at: 1771 2389 1771
Size: 618 5150
HpaI GTT'AAC
Cuts at: 1337 1337
Size: 5768
KasI G'GCGC_C
Cuts at: 3198 3446 3198
Size: 248 5520
KpnI G_GTAC'C
Cuts at: 1420 2035 2788 1420
Size: 615 753 4400
Fragments arranged by size:
4400 753 615
MluI A'CGCG_T
Cuts at: 5201 5201
Size: 5768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1397 2673 4527 4711 1397
Size: 1276 1854 184 2454
Fragments arranged by size:
2454 1854 1276 184
MscI TGG'CCA
Cuts at: 3529 3529
Size: 5768
MslI CAynn'nnrTG
Cuts at: 139 1919 1930 2498 3757 3884 139
Size: 1780 11 568 1259 127 2023
Fragments arranged by size:
2023 1780 1259 568 127 11
MspA1I CmG'CkG
Cuts at: 1625 1842 2879 3193 3553 4337 4582 1625
Size: 217 1037 314 360 784 245 2811
Fragments arranged by size:
2811 1037 784 360 314 245 217
NaeI GCC'GGC
Cuts at: 3949 3949
Size: 5768
NarI GG'CG_CC
Cuts at: 3199 3447 3199
Size: 248 5520
NgoMIV G'CCGG_C
Cuts at: 3947 3947
Size: 5768
NspI r_CATG'y
Cuts at: 652 831 977 1935 2821 3321 4924 652
Size: 179 146 958 886 500 1603 1496
Fragments arranged by size:
1603 1496 958 886 500 179 146
PciI A'CATG_T
Cuts at: 648 4920 648
Size: 4272 1496
PflMI CCAn_nnn'nTGG
Cuts at: 113 316 113
Size: 203 5565
PfoI T'CCnGG_A
Cuts at: 2013 2013
Size: 5768
PmeI GTTT'AAAC
Cuts at: 2767 2767
Size: 5768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 4214
Size: 32 5736
PpuMI rG'GwC_Cy
Cuts at: 904 2047 904
Size: 1143 4625
PshAI GACnn'nnGTC
Cuts at: 2561 2561
Size: 5768
PsiI TTA'TAA
Cuts at: 919 919
Size: 5768
PstI C_TGCA'G
Cuts at: 1847 1865 2515 2815 3500 1847
Size: 18 650 300 685 4115
Fragments arranged by size:
4115 685 650 300 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5768
PvuII CAG'CTG
Cuts at: 1842 3193 3553 1842
Size: 1351 360 4057
Fragments arranged by size:
4057 1351 360
RsrII CG'GwC_CG
Cuts at: 3963 3963
Size: 5768
SacI G_AGCT'C
Cuts at: 436 1702 2782 436
Size: 1266 1080 3422
Fragments arranged by size:
3422 1266 1080
SalI G'TCGA_C
Cuts at: 2805 2805
Size: 5768
SanDI GG'GwC_CC
Cuts at: 2047 2047
Size: 5768
SapI GCTCTTCn'nnn_
Cuts at: 3791 4001 3791
Size: 210 5558
SbfI CC_TGCA'GG
Cuts at: 2815 2815
Size: 5768
ScaI AGT'ACT
Cuts at: 1535 5285 1535
Size: 3750 2018
SfcI C'TryA_G
Cuts at: 33 1843 1861 2511 2559 2705 2811 3496 4464
Size: 1810 18 650 48 146 106 685 968
Cuts at: 4464 4655 33
Size: 191 1146
Fragments arranged by size:
1810 1146 968 685 650 191 146 106
48 18
SfoI GGC'GCC
Cuts at: 3200 3448 3200
Size: 248 5520
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
SmaI CCC'GGG
Cuts at: 2790 2790
Size: 5768
SmlI C'TyrA_G
Cuts at: 435 2679 2923 3838 4552 4814 435
Size: 2244 244 915 714 262 1389
Fragments arranged by size:
2244 1389 915 714 262 244
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5768
SphI G_CATG'C
Cuts at: 2821 3321 2821
Size: 500 5268
SspI AAT'ATT
Cuts at: 698 698
Size: 5768
StuI AGG'CCT
Cuts at: 492 1759 492
Size: 1267 4501
StyI C'CwwG_G
Cuts at: 329 2902 3256 329
Size: 2573 354 2841
Fragments arranged by size:
2841 2573 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3351 3484 3943 4152 3351
Size: 133 459 209 4967
Fragments arranged by size:
4967 459 209 133
TatI w'GTAC_w
Cuts at: 866 1533 2581 5283 866
Size: 667 1048 2702 1351
Fragments arranged by size:
2702 1351 1048 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 870 902 1894 1926 1949 1981 2213 2245 5285
Size: 32 992 32 23 32 232 32 3040
Cuts at: 5285 5317 870
Size: 32 1321
Fragments arranged by size:
3040 1321 992 232 32 32 32 32
32 23
Tth111I GACn'n_nGTC
Cuts at: 296 3565 296
Size: 3269 2499
XbaI T'CTAG_A
Cuts at: 2799 2799
Size: 5768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 135 2311 3263 114
Size: 21 2176 952 2619
Fragments arranged by size:
2619 2176 952 21
XmaI C'CCGG_G
Cuts at: 2788 2788
Size: 5768
XmnI GAAnn'nnTTC
Cuts at: 565 637 1296 565
Size: 72 659 5037
Fragments arranged by size:
5037 659 72
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI