Restriction map for
pF1KB4590
(Circular) (Six-base) MAPSORT of: pF1KB4590.seq Check: 4254 from: 1 to: 5132
pF1KB4590 5132 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 282 2170 282
Size: 1888 3244
Acc65I G'GTAC_C
Cuts at: 2148 2148
Size: 5132
AclI AA'CG_TT
Cuts at: 4972 4972
Size: 5132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 155 515 1036 1043 2957 3389 3742 4404
Size: 18 360 521 7 1914 432 353 662
Cuts at: 4404 137
Size: 865
Fragments arranged by size:
1914 865 662 521 432 360 353 18
7
AflII C'TTAA_G
Cuts at: 352 352
Size: 5132
AflIII A'CryG_T
Cuts at: 2066 2484 4284 4565 2066
Size: 418 1800 281 2633
Fragments arranged by size:
2633 1800 418 281
AleI CACnn'nnGTG
Cuts at: 2080 2080
Size: 5132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1085 1085
Size: 5132
AlwNI CAG_nnn'CTG
Cuts at: 2025 2437 3875 2025
Size: 412 1438 3282
Fragments arranged by size:
3282 1438 412
ApaLI G'TGCA_C
Cuts at: 3970 3970
Size: 5132
ApoI r'AATT_y
Cuts at: 1186 1448 1648 1955 2136 4485 4741 1186
Size: 262 200 307 181 2349 256 1577
Fragments arranged by size:
2349 1577 307 262 256 200 181
AseI AT'TA_AT
Cuts at: 20 20
Size: 5132
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
AvaI C'yCGr_G
Cuts at: 2152 2152
Size: 5132
BamHI G'GATC_C
Cuts at: 1117 2157 1117
Size: 1040 4092
BanI G'GyrC_C
Cuts at: 787 2148 2562 2810 2845 787
Size: 1361 414 248 35 3074
Fragments arranged by size:
3074 1361 414 248 35
BanII G_rGCy'C
Cuts at: 1770 2146 3176 1770
Size: 376 1030 3726
Fragments arranged by size:
3726 1030 376
BbeI G_GCGC'C
Cuts at: 2566 2814 2566
Size: 248 4884
BbsI GAAGACnn'nnnn_
Cuts at: 212 659 754 212
Size: 447 95 4590
Fragments arranged by size:
4590 447 95
BbvCI CC'TCA_GC
Cuts at: 2111 2111
Size: 5132
BciVI GTATCCnnnnn_n'
Cuts at: 3030 4086 5132 3030
Size: 1056 1046 3030
Fragments arranged by size:
3030 1056 1046
BclI T'GATC_A
Cuts at: 1988 2010 2652 1988
Size: 22 642 4468
Fragments arranged by size:
4468 642 22
BglII A'GATC_T
Cuts at: 2647 4684 2647
Size: 2037 3095
BlpI GC'TnA_GC
Cuts at: 2244 2244
Size: 5132
Bme1580I G_kGCm'C
Cuts at: 2757 2850 3974 2757
Size: 93 1124 3915
Fragments arranged by size:
3915 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1278 2344 2425 2760 4904 1278
Size: 1066 81 335 2144 1506
Fragments arranged by size:
2144 1506 1066 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 5132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1589 1734 829
Size: 760 145 4227
Fragments arranged by size:
4227 760 145
Bpu10I CC'TnA_GC
Cuts at: 482 908 2030 2111 482
Size: 426 1122 81 3503
Fragments arranged by size:
3503 1122 426 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2308 3187 3901 4199 2308
Size: 879 714 298 3241
Fragments arranged by size:
3241 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 679 1752 4836 679
Size: 1073 3084 975
Fragments arranged by size:
3084 1073 975
BsaAI yAC'GTr
Cuts at: 2485 2485
Size: 5132
BsaBI GATnn'nnATC
Cuts at: 6 2009 2646 4683 6
Size: 2003 637 2037 455
Fragments arranged by size:
2037 2003 637 455
BsaHI Gr'CG_yC
Cuts at: 2563 2811 3513 5009 2563
Size: 248 702 1496 2686
Fragments arranged by size:
2686 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2321 2842 3520 3931 4078 4352 2321
Size: 521 678 411 147 274 3101
Fragments arranged by size:
3101 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 410 683 247
Size: 163 273 4696
Fragments arranged by size:
4696 273 163
BseYI C'CCAG_C
Cuts at: 248 1368 1819 2557 3315 3980 4388 248
Size: 1120 451 738 758 665 408 992
Fragments arranged by size:
1120 992 758 738 665 451 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1225 718
Size: 507 4625
BsiEI CG_ry'CG
Cuts at: 104 2674 2720 3950 104
Size: 2570 46 1230 1286
Fragments arranged by size:
2570 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1770 2146 2924 3114 3974 1770
Size: 376 778 190 860 2928
Fragments arranged by size:
2928 860 778 376 190
Bsp1286I G_dGCh'C
Cuts at: 1770 2146 2757 2850 2924 3114 3176 3974 1770
Size: 376 611 93 74 190 62 798 2928
Fragments arranged by size:
2928 798 611 376 190 93 74 62
BspHI T'CATG_A
Cuts at: 2013 3564 2013
Size: 1551 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2182 2396 2698 3079 2182
Size: 214 302 381 4235
Fragments arranged by size:
4235 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3424 3478 4962 5127 50
Size: 3374 54 1484 165 55
Fragments arranged by size:
3374 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1067 1856 3044 1067
Size: 789 1188 3155
Fragments arranged by size:
3155 1188 789
BsrFI r'CCGG_y
Cuts at: 1488 3130 3311 1488
Size: 1642 181 3309
Fragments arranged by size:
3309 1642 181
BssSI C'ACGA_G
Cuts at: 3403 4111 3403
Size: 708 4424
BstAPI GCAn_nnn'nTGC
Cuts at: 2509 2509
Size: 5132
BstBI TT'CG_AA
Cuts at: 3493 3493
Size: 5132
BstYI r'GATC_y
Cuts at: 1 927 1117 2157 2624 2647 2982 3228 3534
Size: 926 190 1040 467 23 335 246 306
Cuts at: 3534 3546 3632 4684 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1040 926 467 449 335 306 246
190 86 23 12
BtgI C'CryG_G
Cuts at: 346 967 1862 346
Size: 621 895 3616
Fragments arranged by size:
3616 895 621
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3082 3263 3426 3082
Size: 181 163 4788
Fragments arranged by size:
4788 181 163
BtsI GCAGTG_nn'
Cuts at: 2375 2375
Size: 5132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2066 2101 2066
Size: 35 5097
DraI TTT'AAA
Cuts at: 2131 2131
Size: 5132
DrdI GACnn_nn'nnGTC
Cuts at: 1641 2071 2838 4182 1641
Size: 430 767 1344 2591
Fragments arranged by size:
2591 1344 767 430
EaeI y'GGCC_r
Cuts at: 383 1077 1432 1662 2717 2891 3282 3309 4654
Size: 694 355 230 1055 174 391 27 1345
Cuts at: 4654 383
Size: 861
Fragments arranged by size:
1345 1055 861 694 391 355 230 174
27
EagI C'GGCC_G
Cuts at: 2717 2717
Size: 5132
EarI CTCTTCn'nnn_
Cuts at: 289 623 1217 3155 3365 289
Size: 334 594 1938 210 2056
Fragments arranged by size:
2056 1938 594 334 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3340 4082 4228 4942 3340
Size: 742 146 714 3530
Fragments arranged by size:
3530 742 714 146
EcoICRI GAG'CTC
Cuts at: 1768 2144 1768
Size: 376 4756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 155 515 829 1036 1043 1589 1734 2957
Size: 18 360 314 207 7 546 145 1223
Cuts at: 2957 3389 3742 4404 137
Size: 432 353 662 865
Fragments arranged by size:
1223 865 662 546 432 360 353 314
207 145 18 7
EcoO109I rG'GnC_Cy
Cuts at: 2271 2271
Size: 5132
EcoRI G'AATT_C
Cuts at: 1186 2136 1186
Size: 950 4182
EcoRV GAT'ATC
Cuts at: 1029 1029
Size: 5132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3151 3151
Size: 5132
FspI TGC'GCA
Cuts at: 2913 2913
Size: 5132
HaeII r_GCGC'y
Cuts at: 1817 2566 2814 4044 4815 1817
Size: 749 248 1230 771 2134
Fragments arranged by size:
2134 1230 771 749 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 1057 1089 1092 1124 1400 1432 2974
Size: 32 313 32 3 32 276 32 1542
Cuts at: 2974 3004 3006 3036 712
Size: 30 2 30 2808
Fragments arranged by size:
2808 1542 313 276 32 32 32 32
30 30 3 2
HincII GTy'rAC
Cuts at: 1326 2171 1326
Size: 845 4287
KasI G'GCGC_C
Cuts at: 2562 2810 2562
Size: 248 4884
KpnI G_GTAC'C
Cuts at: 2152 2152
Size: 5132
MfeI C'AATT_G
Cuts at: 1015 1015
Size: 5132
MluI A'CGCG_T
Cuts at: 4565 4565
Size: 5132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3891 4075 3891
Size: 184 4948
MscI TGG'CCA
Cuts at: 385 2893 385
Size: 2508 2624
MslI CAynn'nnrTG
Cuts at: 1861 2080 3121 3248 1861
Size: 219 1041 127 3745
Fragments arranged by size:
3745 1041 219 127
MspA1I CmG'CkG
Cuts at: 265 800 2243 2557 2917 3701 3946 265
Size: 535 1443 314 360 784 245 1451
Fragments arranged by size:
1451 1443 784 535 360 314 245
NaeI GCC'GGC
Cuts at: 3313 3313
Size: 5132
NarI GG'CG_CC
Cuts at: 2563 2811 2563
Size: 248 4884
NcoI C'CATG_G
Cuts at: 346 967 346
Size: 621 4511
NgoMIV G'CCGG_C
Cuts at: 3311 3311
Size: 5132
NspI r_CATG'y
Cuts at: 820 2070 2185 2685 4288 820
Size: 1250 115 500 1603 1664
Fragments arranged by size:
1664 1603 1250 500 115
PciI A'CATG_T
Cuts at: 2066 4284 2066
Size: 2218 2914
PflMI CCAn_nnn'nTGG
Cuts at: 2025 2025
Size: 5132
PfoI T'CCnGG_A
Cuts at: 1750 1750
Size: 5132
PmeI GTTT'AAAC
Cuts at: 2131 2131
Size: 5132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 5100
PshAI GACnn'nnGTC
Cuts at: 1790 1790
Size: 5132
PstI C_TGCA'G
Cuts at: 264 1133 2179 2864 264
Size: 869 1046 685 2532
Fragments arranged by size:
2532 1046 869 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5132
PvuII CAG'CTG
Cuts at: 265 800 2557 2917 265
Size: 535 1757 360 2480
Fragments arranged by size:
2480 1757 535 360
RsrII CG'GwC_CG
Cuts at: 3327 3327
Size: 5132
SacI G_AGCT'C
Cuts at: 1770 2146 1770
Size: 376 4756
SalI G'TCGA_C
Cuts at: 2169 2169
Size: 5132
SapI GCTCTTCn'nnn_
Cuts at: 3155 3365 3155
Size: 210 4922
SbfI CC_TGCA'GG
Cuts at: 1133 2179 1133
Size: 1046 4086
ScaI AGT'ACT
Cuts at: 4649 4649
Size: 5132
SfcI C'TryA_G
Cuts at: 33 260 1129 1590 1656 2175 2860 3828 4019
Size: 227 869 461 66 519 685 968 191
Cuts at: 4019 33
Size: 1146
Fragments arranged by size:
1146 968 869 685 519 461 227 191
66
SfoI GGC'GCC
Cuts at: 2564 2812 2564
Size: 248 4884
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
SmaI CCC'GGG
Cuts at: 2154 2154
Size: 5132
SmlI C'TyrA_G
Cuts at: 352 2287 3202 3916 4178 352
Size: 1935 915 714 262 1306
Fragments arranged by size:
1935 1306 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5132
SphI G_CATG'C
Cuts at: 2185 2685 2185
Size: 500 4632
StyI C'CwwG_G
Cuts at: 176 346 689 821 967 1340 1637 2266 2620
Size: 170 343 132 146 373 297 629 354
Cuts at: 2620 176
Size: 2688
Fragments arranged by size:
2688 629 373 354 343 297 170 146
132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1684 2061 2715 2848 3307 3516 1684
Size: 377 654 133 459 209 3300
Fragments arranged by size:
3300 654 459 377 209 133
TatI w'GTAC_w
Cuts at: 4647 4647
Size: 5132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4649 4681 4649
Size: 32 5100
Tth111I GACn'n_nGTC
Cuts at: 2929 2929
Size: 5132
XbaI T'CTAG_A
Cuts at: 1758 2163 1758
Size: 405 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2627 2627
Size: 5132
XmaI C'CCGG_G
Cuts at: 2152 2152
Size: 5132
XmnI GAAnn'nnTTC
Cuts at: 458 458
Size: 5132
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI