Restriction map for
pF1KB4592
(Circular) (Six-base) MAPSORT of: pF1KB4592.seq Check: 2833 from: 1 to: 4916
pF1KB4592 4916 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1201 1508 1954 1201
Size: 307 446 4163
Fragments arranged by size:
4163 446 307
Acc65I G'GTAC_C
Cuts at: 1932 1932
Size: 4916
AclI AA'CG_TT
Cuts at: 4756 4756
Size: 4916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 1052 2741 3173 3526 4188 767
Size: 285 1689 432 353 662 1495
Fragments arranged by size:
1689 1495 662 432 353 285
AflIII A'CryG_T
Cuts at: 1161 2268 4068 4349 1161
Size: 1107 1800 281 1728
Fragments arranged by size:
1800 1728 1107 281
AlwNI CAG_nnn'CTG
Cuts at: 1048 2221 3659 1048
Size: 1173 1438 2305
Fragments arranged by size:
2305 1438 1173
ApaI G_GGCC'C
Cuts at: 1578 1578
Size: 4916
ApaLI G'TGCA_C
Cuts at: 3754 3754
Size: 4916
ApoI r'AATT_y
Cuts at: 366 715 1118 1453 1473 1920 4269 4525 366
Size: 349 403 335 20 447 2349 256 757
Fragments arranged by size:
2349 757 447 403 349 335 256 20
AseI AT'TA_AT
Cuts at: 20 1658 20
Size: 1638 3278
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
AvaI C'yCGr_G
Cuts at: 127 1936 127
Size: 1809 3107
BamHI G'GATC_C
Cuts at: 1941 1941
Size: 4916
BanI G'GyrC_C
Cuts at: 1932 2346 2594 2629 1932
Size: 414 248 35 4219
Fragments arranged by size:
4219 414 248 35
BanII G_rGCy'C
Cuts at: 128 203 953 1578 1930 2960 128
Size: 75 750 625 352 1030 2084
Fragments arranged by size:
2084 1030 750 625 352 75
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4668
BbsI GAAGACnn'nnnn_
Cuts at: 218 1862 218
Size: 1644 3272
BciVI GTATCCnnnnn_n'
Cuts at: 1155 2814 3870 4916 1155
Size: 1659 1056 1046 1155
Fragments arranged by size:
1659 1155 1056 1046
BclI T'GATC_A
Cuts at: 2436 2436
Size: 4916
BglII A'GATC_T
Cuts at: 485 2431 4468 485
Size: 1946 2037 933
Fragments arranged by size:
2037 1946 933
BlpI GC'TnA_GC
Cuts at: 353 546 2028 353
Size: 193 1482 3241
Fragments arranged by size:
3241 1482 193
Bme1580I G_kGCm'C
Cuts at: 1578 2541 2634 3758 1578
Size: 963 93 1124 2736
Fragments arranged by size:
2736 1124 963 93
BmgBI CAC'GTC
Cuts at: 216 691 216
Size: 475 4441
BmrI ACTGGGnnnn_n'
Cuts at: 834 2128 2209 2544 4688 834
Size: 1294 81 335 2144 1062
Fragments arranged by size:
2144 1294 1062 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 4916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 937
Size: 4916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 2092 2971 3685 3983 883
Size: 1209 879 714 298 1816
Fragments arranged by size:
1816 1209 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1093 1727 4620 1093
Size: 634 2893 1389
Fragments arranged by size:
2893 1389 634
BsaAI yAC'GTr
Cuts at: 2269 2269
Size: 4916
BsaBI GATnn'nnATC
Cuts at: 6 1154 1330 2430 4467 6
Size: 1148 176 1100 2037 455
Fragments arranged by size:
2037 1148 1100 455 176
BsaHI Gr'CG_yC
Cuts at: 2347 2595 3297 4793 2347
Size: 248 702 1496 2470
Fragments arranged by size:
2470 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2105 2626 3304 3715 3862 4136 2105
Size: 521 678 411 147 274 2885
Fragments arranged by size:
2885 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1724 1754 1724
Size: 30 4886
BseYI C'CCAG_C
Cuts at: 360 1443 2341 3099 3764 4172 360
Size: 1083 898 758 665 408 1104
Fragments arranged by size:
1104 1083 898 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 4916
BsiEI CG_ry'CG
Cuts at: 104 182 2458 2504 3734 104
Size: 78 2276 46 1230 1286
Fragments arranged by size:
2276 1286 1230 78 46
BsiHKAI G_wGCw'C
Cuts at: 128 203 667 953 1930 2708 2898 3758 128
Size: 75 464 286 977 778 190 860 1286
Fragments arranged by size:
1286 977 860 778 464 286 190 75
Bsp1286I G_dGCh'C
Cuts at: 128 203 667 953 1578 1930 2541 2634 2708
Size: 75 464 286 625 352 611 93 74
Cuts at: 2708 2898 2960 3758 128
Size: 190 62 798 1286
Fragments arranged by size:
1286 798 625 611 464 352 286 190
93 75 74 62
BspHI T'CATG_A
Cuts at: 1541 3348 1541
Size: 1807 3109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1966 2180 2482 2863 1966
Size: 214 302 381 4019
Fragments arranged by size:
4019 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3208 3262 4746 4911 50
Size: 3158 54 1484 165 55
Fragments arranged by size:
3158 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 213 2828 213
Size: 2615 2301
BsrFI r'CCGG_y
Cuts at: 133 2914 3095 133
Size: 2781 181 1954
Fragments arranged by size:
2781 1954 181
BssSI C'ACGA_G
Cuts at: 1495 3187 3895 1495
Size: 1692 708 2516
Fragments arranged by size:
2516 1692 708
BstAPI GCAn_nnn'nTGC
Cuts at: 622 1048 1384 1707 2293 622
Size: 426 336 323 586 3245
Fragments arranged by size:
3245 586 426 336 323
BstBI TT'CG_AA
Cuts at: 3277 3277
Size: 4916
BstYI r'GATC_y
Cuts at: 1 485 1941 2408 2431 2766 3012 3318 3330
Size: 484 1456 467 23 335 246 306 12
Cuts at: 3330 3416 4468 1
Size: 86 1052 449
Fragments arranged by size:
1456 1052 484 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1509 1509
Size: 4916
BtgI C'CryG_G
Cuts at: 107 962 107
Size: 855 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4572
Fragments arranged by size:
4572 181 163
BtsI GCAGTG_nn'
Cuts at: 2159 2159
Size: 4916
DraI TTT'AAA
Cuts at: 1915 1915
Size: 4916
DrdI GACnn_nn'nnGTC
Cuts at: 1864 2622 3966 1864
Size: 758 1344 2814
Fragments arranged by size:
2814 1344 758
EaeI y'GGCC_r
Cuts at: 1406 1763 2501 2675 3066 3093 4438 1406
Size: 357 738 174 391 27 1345 1884
Fragments arranged by size:
1884 1345 738 391 357 174 27
EagI C'GGCC_G
Cuts at: 2501 2501
Size: 4916
EarI CTCTTCn'nnn_
Cuts at: 437 2939 3149 437
Size: 2502 210 2204
Fragments arranged by size:
2502 2204 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1390 3124 3866 4012 4726 1390
Size: 1734 742 146 714 1580
Fragments arranged by size:
1734 1580 742 714 146
EcoICRI GAG'CTC
Cuts at: 126 201 951 1928 126
Size: 75 750 977 3114
Fragments arranged by size:
3114 977 750 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 937 1052 2741 3173 3526 4188 767
Size: 170 115 1689 432 353 662 1495
Fragments arranged by size:
1689 1495 662 432 353 170 115
EcoO109I rG'GnC_Cy
Cuts at: 281 388 967 2055 281
Size: 107 579 1088 3142
Fragments arranged by size:
3142 1088 579 107
EcoRI G'AATT_C
Cuts at: 1453 1473 1920 1453
Size: 20 447 4449
Fragments arranged by size:
4449 447 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4916
FspI TGC'GCA
Cuts at: 2697 2697
Size: 4916
HaeII r_GCGC'y
Cuts at: 2350 2598 3828 4599 2350
Size: 248 1230 771 2667
Fragments arranged by size:
2667 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2758 2788 2790 2820 2758
Size: 30 2 30 4854
Fragments arranged by size:
4854 30 30 2
HincII GTy'rAC
Cuts at: 1955 1955
Size: 4916
HindIII A'AGCT_T
Cuts at: 518 821 518
Size: 303 4613
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4668
KpnI G_GTAC'C
Cuts at: 1936 1936
Size: 4916
MfeI C'AATT_G
Cuts at: 254 254
Size: 4916
MluI A'CGCG_T
Cuts at: 4349 4349
Size: 4916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 249 3675 3859 249
Size: 3426 184 1306
Fragments arranged by size:
3426 1306 184
MscI TGG'CCA
Cuts at: 1408 1765 2677 1408
Size: 357 912 3647
Fragments arranged by size:
3647 912 357
MslI CAynn'nnrTG
Cuts at: 582 679 1108 2905 3032 582
Size: 97 429 1797 127 2466
Fragments arranged by size:
2466 1797 429 127 97
MspA1I CmG'CkG
Cuts at: 148 360 1045 1428 1443 2027 2341 2701 3485
Size: 212 685 383 15 584 314 360 784
Cuts at: 3485 3730 148
Size: 245 1334
Fragments arranged by size:
1334 784 685 584 383 360 314 245
212 15
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 4916
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4668
NcoI C'CATG_G
Cuts at: 107 962 107
Size: 855 4061
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 4916
NspI r_CATG'y
Cuts at: 587 1165 1969 2469 4072 587
Size: 578 804 500 1603 1431
Fragments arranged by size:
1603 1431 804 578 500
PasI CC'CwG_GG
Cuts at: 611 611
Size: 4916
PciI A'CATG_T
Cuts at: 1161 4068 1161
Size: 2907 2009
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4884
PpuMI rG'GwC_Cy
Cuts at: 281 388 967 281
Size: 107 579 4230
Fragments arranged by size:
4230 579 107
PspOMI G'GGCC_C
Cuts at: 1574 1574
Size: 4916
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1223 1255 1733 1765 1223
Size: 32 478 32 4374
Fragments arranged by size:
4374 478 32 32
PstI C_TGCA'G
Cuts at: 1963 2648 1963
Size: 685 4231
PvuI CG_AT'CG
Cuts at: 104 182 104
Size: 78 4838
PvuII CAG'CTG
Cuts at: 360 1045 1443 2341 2701 360
Size: 685 398 898 360 2575
Fragments arranged by size:
2575 898 685 398 360
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4916
SacI G_AGCT'C
Cuts at: 128 203 953 1930 128
Size: 75 750 977 3114
Fragments arranged by size:
3114 977 750 75
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4916
SapI GCTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4706
SbfI CC_TGCA'GG
Cuts at: 1963 1963
Size: 4916
ScaI AGT'ACT
Cuts at: 1832 4433 1832
Size: 2601 2315
SfcI C'TryA_G
Cuts at: 33 1834 1959 2644 3612 3803 33
Size: 1801 125 685 968 191 1146
Fragments arranged by size:
1801 1146 968 685 191 125
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
SmaI CCC'GGG
Cuts at: 1938 1938
Size: 4916
SmlI C'TyrA_G
Cuts at: 862 2071 2986 3700 3962 862
Size: 1209 915 714 262 1816
Fragments arranged by size:
1816 1209 915 714 262
SpeI A'CTAG_T
Cuts at: 67 772 1548 67
Size: 705 776 3435
Fragments arranged by size:
3435 776 705
SphI G_CATG'C
Cuts at: 1969 2469 1969
Size: 500 4416
StuI AGG'CCT
Cuts at: 1675 1675
Size: 4916
StyI C'CwwG_G
Cuts at: 107 962 2050 2404 107
Size: 855 1088 354 2619
Fragments arranged by size:
2619 1088 855 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 700 1435 2499 2632 3091 3300 700
Size: 735 1064 133 459 209 2316
Fragments arranged by size:
2316 1064 735 459 209 133
TatI w'GTAC_w
Cuts at: 1830 4431 1830
Size: 2601 2315
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 273 305 4433 4465 273
Size: 32 4128 32 724
Fragments arranged by size:
4128 724 32 32
Tth111I GACn'n_nGTC
Cuts at: 2713 2713
Size: 4916
XbaI T'CTAG_A
Cuts at: 1794 1947 1794
Size: 153 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1371 2411 1371
Size: 1040 3876
XmaI C'CCGG_G
Cuts at: 1936 1936
Size: 4916
XmnI GAAnn'nnTTC
Cuts at: 758 1235 758
Size: 477 4439
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI