Restriction map for
pF1KB4606
(Circular) (Six-base) MAPSORT of: pF1KB4606.seq Check: 7526 from: 1 to: 3482
pF1KB4606 3482 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 149 149
Size: 3482
AccI GT'mk_AC
Cuts at: 112 520 112
Size: 408 3074
Acc65I G'GTAC_C
Cuts at: 498 498
Size: 3482
AclI AA'CG_TT
Cuts at: 3322 3322
Size: 3482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 1739 2486 2754 1307
Size: 432 747 268 2035
Fragments arranged by size:
2035 747 432 268
AflIII A'CryG_T
Cuts at: 834 1938 2915 834
Size: 1104 977 1401
Fragments arranged by size:
1401 1104 977
AgeI A'CCGG_T
Cuts at: 1870 1870
Size: 3482
AlwNI CAG_nnn'CTG
Cuts at: 138 787 2354 138
Size: 649 1567 1266
Fragments arranged by size:
1567 1266 649
ApaLI G'TGCA_C
Cuts at: 2252 2252
Size: 3482
ApoI r'AATT_y
Cuts at: 486 2835 3091 486
Size: 2349 256 877
Fragments arranged by size:
2349 877 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
AvaI C'yCGr_G
Cuts at: 502 502
Size: 3482
BamHI G'GATC_C
Cuts at: 507 507
Size: 3482
BanI G'GyrC_C
Cuts at: 185 498 912 1160 1195 185
Size: 313 414 248 35 2472
Fragments arranged by size:
2472 414 313 248 35
BanII G_rGCy'C
Cuts at: 496 1526 496
Size: 1030 2452
BbeI G_GCGC'C
Cuts at: 189 916 1164 189
Size: 727 248 2507
Fragments arranged by size:
2507 727 248
BbsI GAAGACnn'nnnn_
Cuts at: 141 149 141
Size: 8 3474
BciVI GTATCCnnnnn_n'
Cuts at: 1380 2141 3482 1380
Size: 761 1341 1380
Fragments arranged by size:
1380 1341 761
BclI T'GATC_A
Cuts at: 1002 1002
Size: 3482
BglII A'GATC_T
Cuts at: 997 3034 997
Size: 2037 1445
BlpI GC'TnA_GC
Cuts at: 203 594 203
Size: 391 3091
Bme1580I G_kGCm'C
Cuts at: 1107 1200 2256 1107
Size: 93 1056 2333
Fragments arranged by size:
2333 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 694 775 1110 3254 694
Size: 81 335 2144 922
Fragments arranged by size:
2144 922 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1537 2029 2327 658
Size: 879 492 298 1813
Fragments arranged by size:
1813 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3186 3186
Size: 3482
BsaAI yAC'GTr
Cuts at: 835 835
Size: 3482
BsaBI GATnn'nnATC
Cuts at: 6 996 3033 6
Size: 990 2037 455
Fragments arranged by size:
2037 990 455
BsaHI Gr'CG_yC
Cuts at: 146 186 913 1161 1863 3359 146
Size: 40 727 248 702 1496 269
Fragments arranged by size:
1496 727 702 269 248 40
BsaWI w'CCGG_w
Cuts at: 671 1192 1870 2144 2291 2702 671
Size: 521 678 274 147 411 1451
Fragments arranged by size:
1451 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 457 907 1665 2242 2738 457
Size: 450 758 577 496 1201
Fragments arranged by size:
1201 758 577 496 450
BsiEI CG_ry'CG
Cuts at: 104 1024 1070 2278 104
Size: 920 46 1208 1308
Fragments arranged by size:
1308 1208 920 46
BsiHKAI G_wGCw'C
Cuts at: 319 496 1274 1464 2256 319
Size: 177 778 190 792 1545
Fragments arranged by size:
1545 792 778 190 177
BsmBI CGTCTCn'nnnn_
Cuts at: 327 327
Size: 3482
Bsp1286I G_dGCh'C
Cuts at: 319 496 1107 1200 1274 1464 1526 2256 319
Size: 177 611 93 74 190 62 730 1545
Fragments arranged by size:
1545 730 611 190 177 93 74 62
BspEI T'CCGG_A
Cuts at: 2702 2702
Size: 3482
BspHI T'CATG_A
Cuts at: 2658 2658
Size: 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 532 746 1048 1429 532
Size: 214 302 381 2585
Fragments arranged by size:
2585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1774 1828 3312 3477 50
Size: 1724 54 1484 165 55
Fragments arranged by size:
1724 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 1394
Size: 3482
BsrFI r'CCGG_y
Cuts at: 1480 1661 1870 1480
Size: 181 209 3092
Fragments arranged by size:
3092 209 181
BssSI C'ACGA_G
Cuts at: 1753 2111 1753
Size: 358 3124
BstAPI GCAn_nnn'nTGC
Cuts at: 859 859
Size: 3482
BstBI TT'CG_AA
Cuts at: 1843 1843
Size: 3482
BstYI r'GATC_y
Cuts at: 1 507 974 997 1332 1578 2590 2676 2688
Size: 506 467 23 335 246 1012 86 12
Cuts at: 2688 3034 1
Size: 346 449
Fragments arranged by size:
1012 506 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1432 1613 1776 1432
Size: 181 163 3138
Fragments arranged by size:
3138 181 163
BtsI GCAGTG_nn'
Cuts at: 725 725
Size: 3482
DraI TTT'AAA
Cuts at: 481 481
Size: 3482
DrdI GACnn_nn'nnGTC
Cuts at: 1188 2046 1188
Size: 858 2624
EaeI y'GGCC_r
Cuts at: 302 1067 1241 1632 1659 3004 302
Size: 765 174 391 27 1345 780
Fragments arranged by size:
1345 780 765 391 174 27
EagI C'GGCC_G
Cuts at: 1067 1067
Size: 3482
EarI CTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1690 2000 2146 3292 1690
Size: 310 146 1146 1880
Fragments arranged by size:
1880 1146 310 146
EcoICRI GAG'CTC
Cuts at: 494 494
Size: 3482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1307 1739 2486 2754 1307
Size: 432 747 268 2035
Fragments arranged by size:
2035 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 213 621 213
Size: 408 3074
EcoRI G'AATT_C
Cuts at: 486 486
Size: 3482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1501 1501
Size: 3482
FspI TGC'GCA
Cuts at: 1263 1263
Size: 3482
HaeII r_GCGC'y
Cuts at: 189 916 1164 2186 3165 189
Size: 727 248 1022 979 506
Fragments arranged by size:
1022 979 727 506 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1324 1354 1356 1386 1324
Size: 30 2 30 3420
Fragments arranged by size:
3420 30 30 2
HincII GTy'rAC
Cuts at: 521 521
Size: 3482
KasI G'GCGC_C
Cuts at: 185 912 1160 185
Size: 727 248 2507
Fragments arranged by size:
2507 727 248
KpnI G_GTAC'C
Cuts at: 502 502
Size: 3482
MfeI C'AATT_G
Cuts at: 180 180
Size: 3482
MluI A'CGCG_T
Cuts at: 2915 2915
Size: 3482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2337 2153
Size: 184 3298
MscI TGG'CCA
Cuts at: 304 1243 304
Size: 939 2543
MslI CAynn'nnrTG
Cuts at: 271 298 310 347 1471 1598 271
Size: 27 12 37 1124 127 2155
Fragments arranged by size:
2155 1124 127 37 27 12
MspA1I CmG'CkG
Cuts at: 202 593 907 1267 2280 2525 202
Size: 391 314 360 1013 245 1159
Fragments arranged by size:
1159 1013 391 360 314 245
NaeI GCC'GGC
Cuts at: 1663 1663
Size: 3482
NarI GG'CG_CC
Cuts at: 186 913 1161 186
Size: 727 248 2507
Fragments arranged by size:
2507 727 248
NgoMIV G'CCGG_C
Cuts at: 1661 1661
Size: 3482
NspI r_CATG'y
Cuts at: 535 1035 1942 535
Size: 500 907 2075
Fragments arranged by size:
2075 907 500
PciI A'CATG_T
Cuts at: 1938 1938
Size: 3482
PmeI GTTT'AAAC
Cuts at: 481 481
Size: 3482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2621 2653 2621
Size: 32 3450
PstI C_TGCA'G
Cuts at: 529 1214 529
Size: 685 2797
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3482
PvuII CAG'CTG
Cuts at: 202 907 1267 202
Size: 705 360 2417
Fragments arranged by size:
2417 705 360
RsrII CG'GwC_CG
Cuts at: 1677 1677
Size: 3482
SacI G_AGCT'C
Cuts at: 496 496
Size: 3482
SalI G'TCGA_C
Cuts at: 519 519
Size: 3482
SapI GCTCTTCn'nnn_
Cuts at: 1505 1715 1505
Size: 210 3272
SbfI CC_TGCA'GG
Cuts at: 529 529
Size: 3482
ScaI AGT'ACT
Cuts at: 2999 2999
Size: 3482
SfcI C'TryA_G
Cuts at: 33 525 1210 2203 2394 33
Size: 492 685 993 191 1121
Fragments arranged by size:
1121 993 685 492 191
SfoI GGC'GCC
Cuts at: 187 914 1162 187
Size: 727 248 2507
Fragments arranged by size:
2507 727 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3482
SmaI CCC'GGG
Cuts at: 504 504
Size: 3482
SmlI C'TyrA_G
Cuts at: 637 1552 2044 2306 637
Size: 915 492 262 1813
Fragments arranged by size:
1813 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3482
SphI G_CATG'C
Cuts at: 535 1035 535
Size: 500 2982
StyI C'CwwG_G
Cuts at: 616 970 616
Size: 354 3128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1065 1198 1657 1866 1065
Size: 133 459 209 2681
Fragments arranged by size:
2681 459 209 133
TatI w'GTAC_w
Cuts at: 365 392 2997 365
Size: 27 2605 850
Fragments arranged by size:
2605 850 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3450
Tth111I GACn'n_nGTC
Cuts at: 1279 1279
Size: 3482
XbaI T'CTAG_A
Cuts at: 414 513 414
Size: 99 3383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 445 977 445
Size: 532 2950
XmaI C'CCGG_G
Cuts at: 502 502
Size: 3482
ZraI GAC'GTC
Cuts at: 147 147
Size: 3482
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI