Restriction map for
pF1KB4613
(Circular) (Six-base) MAPSORT of: pF1KB4613.seq Check: 7273 from: 1 to: 3908
pF1KB4613 3908 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 946 946
Size: 3908
Acc65I G'GTAC_C
Cuts at: 924 924
Size: 3908
AclI AA'CG_TT
Cuts at: 3748 3748
Size: 3908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1733 2165 2912 3180 565
Size: 1168 432 747 268 1293
Fragments arranged by size:
1293 1168 747 432 268
AflIII A'CryG_T
Cuts at: 476 533 1260 2364 3341 476
Size: 57 727 1104 977 1043
Fragments arranged by size:
1104 1043 977 727 57
AgeI A'CCGG_T
Cuts at: 2296 2296
Size: 3908
AlwNI CAG_nnn'CTG
Cuts at: 225 1213 2780 225
Size: 988 1567 1353
Fragments arranged by size:
1567 1353 988
ApaLI G'TGCA_C
Cuts at: 163 2678 163
Size: 2515 1393
ApoI r'AATT_y
Cuts at: 912 3261 3517 912
Size: 2349 256 1303
Fragments arranged by size:
2349 1303 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
AvaI C'yCGr_G
Cuts at: 588 928 588
Size: 340 3568
BamHI G'GATC_C
Cuts at: 933 933
Size: 3908
BanI G'GyrC_C
Cuts at: 460 835 924 1338 1586 1621 460
Size: 375 89 414 248 35 2747
Fragments arranged by size:
2747 414 375 248 89 35
BanII G_rGCy'C
Cuts at: 678 922 1952 678
Size: 244 1030 2634
Fragments arranged by size:
2634 1030 244
BbeI G_GCGC'C
Cuts at: 839 1342 1590 839
Size: 503 248 3157
Fragments arranged by size:
3157 503 248
BciVI GTATCCnnnnn_n'
Cuts at: 1806 2567 3908 1806
Size: 761 1341 1806
Fragments arranged by size:
1806 1341 761
BclI T'GATC_A
Cuts at: 1428 1428
Size: 3908
BglI GCCn_nnn'nGGC
Cuts at: 329 329
Size: 3908
BglII A'GATC_T
Cuts at: 1423 3460 1423
Size: 2037 1871
BlpI GC'TnA_GC
Cuts at: 271 520 1020 271
Size: 249 500 3159
Fragments arranged by size:
3159 500 249
Bme1580I G_kGCm'C
Cuts at: 167 463 1533 1626 2682 167
Size: 296 1070 93 1056 1393
Fragments arranged by size:
1393 1070 1056 296 93
BmgBI CAC'GTC
Cuts at: 444 444
Size: 3908
BmrI ACTGGGnnnn_n'
Cuts at: 433 1120 1201 1536 3680 433
Size: 687 81 335 2144 661
Fragments arranged by size:
2144 687 661 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 597 744 2275 597
Size: 147 1531 2230
Fragments arranged by size:
2230 1531 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 403 700 772 132
Size: 271 297 72 3268
Fragments arranged by size:
3268 297 271 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1963 2455 2753 1084
Size: 879 492 298 2239
Fragments arranged by size:
2239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3612 3612
Size: 3908
BsaAI yAC'GTr
Cuts at: 1261 1261
Size: 3908
BsaBI GATnn'nnATC
Cuts at: 6 1422 3459 6
Size: 1416 2037 455
Fragments arranged by size:
2037 1416 455
BsaHI Gr'CG_yC
Cuts at: 836 892 1339 1587 2289 3785 836
Size: 56 447 248 702 1496 959
Fragments arranged by size:
1496 959 702 447 248 56
BsaWI w'CCGG_w
Cuts at: 1097 1618 2296 2570 2717 3128 1097
Size: 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 586
Size: 3908
BseYI C'CCAG_C
Cuts at: 1333 2091 2668 3164 1333
Size: 758 577 496 2077
Fragments arranged by size:
2077 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 907 250
Size: 657 3251
BsiEI CG_ry'CG
Cuts at: 104 116 1450 1496 2704 104
Size: 12 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 167 922 1700 1890 2682 167
Size: 755 778 190 792 1393
Fragments arranged by size:
1393 792 778 755 190
Bsp1286I G_dGCh'C
Cuts at: 167 463 678 922 1533 1626 1700 1890 1952
Size: 296 215 244 611 93 74 190 62
Cuts at: 1952 2682 167
Size: 730 1393
Fragments arranged by size:
1393 730 611 296 244 215 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3128 3128
Size: 3908
BspHI T'CATG_A
Cuts at: 3084 3084
Size: 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 958 1172 1474 1855 491
Size: 467 214 302 381 2544
Fragments arranged by size:
2544 467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 270 2200 2254 3738 3903 50
Size: 220 1930 54 1484 165 55
Fragments arranged by size:
1930 1484 220 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1820 1820
Size: 3908
BsrFI r'CCGG_y
Cuts at: 1906 2087 2296 1906
Size: 181 209 3518
Fragments arranged by size:
3518 209 181
BssHII G'CGCG_C
Cuts at: 332 332
Size: 3908
BssSI C'ACGA_G
Cuts at: 600 2179 2537 600
Size: 1579 358 1971
Fragments arranged by size:
1971 1579 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1285 1285
Size: 3908
BstBI TT'CG_AA
Cuts at: 2269 2269
Size: 3908
BstYI r'GATC_y
Cuts at: 1 933 1400 1423 1758 2004 3016 3102 3114
Size: 932 467 23 335 246 1012 86 12
Cuts at: 3114 3460 1
Size: 346 449
Fragments arranged by size:
1012 932 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 879 107
Size: 772 3136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1858 2039 2202 1858
Size: 181 163 3564
Fragments arranged by size:
3564 181 163
BtsI GCAGTG_nn'
Cuts at: 1151 1151
Size: 3908
DraI TTT'AAA
Cuts at: 907 907
Size: 3908
DraIII CAC_nnn'GTG
Cuts at: 885 885
Size: 3908
DrdI GACnn_nn'nnGTC
Cuts at: 120 1614 2472 120
Size: 1494 858 1556
Fragments arranged by size:
1556 1494 858
EaeI y'GGCC_r
Cuts at: 321 1493 1667 2058 2085 3430 321
Size: 1172 174 391 27 1345 799
Fragments arranged by size:
1345 1172 799 391 174 27
EagI C'GGCC_G
Cuts at: 1493 1493
Size: 3908
EarI CTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 305 429 592 2116 2426 2572 3718 305
Size: 124 163 1524 310 146 1146 495
Fragments arranged by size:
1524 1146 495 310 163 146 124
EcoICRI GAG'CTC
Cuts at: 920 920
Size: 3908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 403 565 700 772 1733 2165 2912 3180
Size: 271 162 135 72 961 432 747 268
Cuts at: 3180 132
Size: 860
Fragments arranged by size:
961 860 747 432 271 268 162 135
72
EcoNI CCTnn'n_nnAGG
Cuts at: 229 818 229
Size: 589 3319
EcoO109I rG'GnC_Cy
Cuts at: 510 1047 510
Size: 537 3371
EcoRI G'AATT_C
Cuts at: 912 912
Size: 3908
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1927 1927
Size: 3908
FspI TGC'GCA
Cuts at: 1689 1689
Size: 3908
HaeII r_GCGC'y
Cuts at: 155 248 839 1342 1590 2612 3591 155
Size: 93 591 503 248 1022 979 472
Fragments arranged by size:
1022 979 591 503 472 248 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 254 286 334 366 571 603 798 830 1750
Size: 32 48 32 205 32 195 32 920
Cuts at: 1750 1780 1782 1812 254
Size: 30 2 30 2350
Fragments arranged by size:
2350 920 205 195 48 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 947 947
Size: 3908
KasI G'GCGC_C
Cuts at: 835 1338 1586 835
Size: 503 248 3157
Fragments arranged by size:
3157 503 248
KpnI G_GTAC'C
Cuts at: 928 928
Size: 3908
MluI A'CGCG_T
Cuts at: 3341 3341
Size: 3908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 548 2579 2763 548
Size: 2031 184 1693
Fragments arranged by size:
2031 1693 184
MscI TGG'CCA
Cuts at: 323 1669 323
Size: 1346 2562
MslI CAynn'nnrTG
Cuts at: 658 1897 2024 658
Size: 1239 127 2542
Fragments arranged by size:
2542 1239 127
MspA1I CmG'CkG
Cuts at: 411 519 750 1019 1333 1693 2706 2951 411
Size: 108 231 269 314 360 1013 245 1368
Fragments arranged by size:
1368 1013 360 314 269 245 231 108
NaeI GCC'GGC
Cuts at: 2089 2089
Size: 3908
NarI GG'CG_CC
Cuts at: 836 1339 1587 836
Size: 503 248 3157
Fragments arranged by size:
3157 503 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3908
NgoMIV G'CCGG_C
Cuts at: 2087 2087
Size: 3908
NspI r_CATG'y
Cuts at: 189 537 961 1461 2368 189
Size: 348 424 500 907 1729
Fragments arranged by size:
1729 907 500 424 348
PciI A'CATG_T
Cuts at: 533 2364 533
Size: 1831 2077
PfoI T'CCnGG_A
Cuts at: 816 816
Size: 3908
PmeI GTTT'AAAC
Cuts at: 907 907
Size: 3908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 3876
PshAI GACnn'nnGTC
Cuts at: 895 895
Size: 3908
PstI C_TGCA'G
Cuts at: 254 502 518 955 1640 254
Size: 248 16 437 685 2522
Fragments arranged by size:
2522 685 437 248 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3908
PvuII CAG'CTG
Cuts at: 519 750 1333 1693 519
Size: 231 583 360 2734
Fragments arranged by size:
2734 583 360 231
RsrII CG'GwC_CG
Cuts at: 2103 2103
Size: 3908
SacI G_AGCT'C
Cuts at: 922 922
Size: 3908
SalI G'TCGA_C
Cuts at: 945 945
Size: 3908
SapI GCTCTTCn'nnn_
Cuts at: 1931 2141 1931
Size: 210 3698
SbfI CC_TGCA'GG
Cuts at: 955 955
Size: 3908
ScaI AGT'ACT
Cuts at: 3425 3425
Size: 3908
SfcI C'TryA_G
Cuts at: 33 132 250 498 514 951 1636 2629 2820
Size: 99 118 248 16 437 685 993 191
Cuts at: 2820 33
Size: 1121
Fragments arranged by size:
1121 993 685 437 248 191 118 99
16
SfoI GGC'GCC
Cuts at: 837 1340 1588 837
Size: 503 248 3157
Fragments arranged by size:
3157 503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3908
SmaI CCC'GGG
Cuts at: 930 930
Size: 3908
SmlI C'TyrA_G
Cuts at: 1063 1978 2470 2732 1063
Size: 915 492 262 2239
Fragments arranged by size:
2239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3908
SphI G_CATG'C
Cuts at: 189 961 1461 189
Size: 772 500 2636
Fragments arranged by size:
2636 772 500
StyI C'CwwG_G
Cuts at: 107 1042 1396 107
Size: 935 354 2619
Fragments arranged by size:
2619 935 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 341 1491 1624 2083 2292 341
Size: 1150 133 459 209 1957
Fragments arranged by size:
1957 1150 459 209 133
TatI w'GTAC_w
Cuts at: 3423 3423
Size: 3908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 3876
Tth111I GACn'n_nGTC
Cuts at: 1705 1705
Size: 3908
XbaI T'CTAG_A
Cuts at: 939 939
Size: 3908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1403 1403
Size: 3908
XmaI C'CCGG_G
Cuts at: 928 928
Size: 3908
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI