Restriction map for
pF1KB4614
(Circular) (Six-base) MAPSORT of: pF1KB4614.seq Check: 3901 from: 1 to: 4901
pF1KB4614 4901 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1939 1939
Size: 4901
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4901
AclI AA'CG_TT
Cuts at: 4741 4741
Size: 4901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1022 2726 3158 3905 4173 932
Size: 90 1704 432 747 268 1660
Fragments arranged by size:
1704 1660 747 432 268 90
AfeI AGC'GCT
Cuts at: 693 844 1245 693
Size: 151 401 4349
Fragments arranged by size:
4349 401 151
AflIII A'CryG_T
Cuts at: 1754 2253 3357 4334 1754
Size: 499 1104 977 2321
Fragments arranged by size:
2321 1104 977 499
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AleI CACnn'nnGTG
Cuts at: 739 1318 739
Size: 579 4322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 668 668
Size: 4901
AlwNI CAG_nnn'CTG
Cuts at: 2206 3773 2206
Size: 1567 3334
ApaLI G'TGCA_C
Cuts at: 3671 3671
Size: 4901
ApoI r'AATT_y
Cuts at: 1435 1525 1732 1890 1905 4254 4510 1435
Size: 90 207 158 15 2349 256 1826
Fragments arranged by size:
2349 1826 256 207 158 90 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4901
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 1087 1921 1087
Size: 834 4067
BamHI G'GATC_C
Cuts at: 787 1165 1926 787
Size: 378 761 3762
Fragments arranged by size:
3762 761 378
BanI G'GyrC_C
Cuts at: 489 1266 1424 1917 2331 2579 2614 489
Size: 777 158 493 414 248 35 2776
Fragments arranged by size:
2776 777 493 414 248 158 35
BanII G_rGCy'C
Cuts at: 1500 1915 2945 1500
Size: 415 1030 3456
Fragments arranged by size:
3456 1030 415
BbeI G_GCGC'C
Cuts at: 2335 2583 2335
Size: 248 4653
BbsI GAAGACnn'nnnn_
Cuts at: 506 920 506
Size: 414 4487
BciVI GTATCCnnnnn_n'
Cuts at: 900 2799 3560 4901 900
Size: 1899 761 1341 900
Fragments arranged by size:
1899 1341 900 761
BclI T'GATC_A
Cuts at: 2421 2421
Size: 4901
BglII A'GATC_T
Cuts at: 2416 4453 2416
Size: 2037 2864
BlpI GC'TnA_GC
Cuts at: 1331 2013 1331
Size: 682 4219
Bme1580I G_kGCm'C
Cuts at: 431 2526 2619 3675 431
Size: 2095 93 1056 1657
Fragments arranged by size:
2095 1657 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 986 1420 2113 2194 2529 4673 986
Size: 434 693 81 335 2144 1214
Fragments arranged by size:
2144 1214 693 434 335 81
BmtI G_CTAG'C
Cuts at: 769 769
Size: 4901
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 4901
Bpu10I CC'TnA_GC
Cuts at: 377 377
Size: 4901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 2956 3448 3746 2077
Size: 879 492 298 3232
Fragments arranged by size:
3232 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4605 4605
Size: 4901
BsaAI yAC'GTr
Cuts at: 2254 2254
Size: 4901
BsaBI GATnn'nnATC
Cuts at: 6 2415 4452 6
Size: 2409 2037 455
Fragments arranged by size:
2409 2037 455
BsaHI Gr'CG_yC
Cuts at: 921 1175 2332 2580 3282 4778 921
Size: 254 1157 248 702 1496 1044
Fragments arranged by size:
1496 1157 1044 702 254 248
BsaWI w'CCGG_w
Cuts at: 2090 2611 3289 3563 3710 4121 2090
Size: 521 678 274 147 411 2870
Fragments arranged by size:
2870 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 608 1501 608
Size: 893 4008
BseYI C'CCAG_C
Cuts at: 138 561 1295 2326 3084 3661 4157 138
Size: 423 734 1031 758 577 496 882
Fragments arranged by size:
1031 882 758 734 577 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 668 350
Size: 318 4583
BsiEI CG_ry'CG
Cuts at: 104 2443 2489 3697 104
Size: 2339 46 1208 1308
Fragments arranged by size:
2339 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1337 1915 2693 2883 3675 1337
Size: 578 778 190 792 2563
Fragments arranged by size:
2563 792 778 578 190
Bsp1286I G_dGCh'C
Cuts at: 431 1337 1500 1915 2526 2619 2693 2883 2945
Size: 906 163 415 611 93 74 190 62
Cuts at: 2945 3675 431
Size: 730 1657
Fragments arranged by size:
1657 906 730 611 415 190 163 93
74 62
BspEI T'CCGG_A
Cuts at: 4121 4121
Size: 4901
BspHI T'CATG_A
Cuts at: 4077 4077
Size: 4901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1951 2165 2467 2848 1951
Size: 214 302 381 4004
Fragments arranged by size:
4004 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3193 3247 4731 4896 50
Size: 3143 54 1484 165 55
Fragments arranged by size:
3143 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2813 2813
Size: 4901
BsrFI r'CCGG_y
Cuts at: 381 2899 3080 3289 381
Size: 2518 181 209 1993
Fragments arranged by size:
2518 1993 209 181
BsrGI T'GTAC_A
Cuts at: 325 325
Size: 4901
BssSI C'ACGA_G
Cuts at: 3172 3530 3172
Size: 358 4543
BstAPI GCAn_nnn'nTGC
Cuts at: 364 2278 364
Size: 1914 2987
BstBI TT'CG_AA
Cuts at: 3262 3262
Size: 4901
BstEII G'GTnAC_C
Cuts at: 1125 1125
Size: 4901
BstXI CCAn_nnnn'nTGG
Cuts at: 146 976 146
Size: 830 4071
BstYI r'GATC_y
Cuts at: 1 787 1165 1562 1926 2393 2416 2751 2997
Size: 786 378 397 364 467 23 335 246
Cuts at: 2997 4009 4095 4107 4453 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 786 467 449 397 378 364 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 493 602 718 1722 493
Size: 109 116 1004 3672
Fragments arranged by size:
3672 1004 116 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 263 2851 3032 3195 263
Size: 2588 181 163 1969
Fragments arranged by size:
2588 1969 181 163
BtsI GCAGTG_nn'
Cuts at: 1449 2144 1449
Size: 695 4206
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DraIII CAC_nnn'GTG
Cuts at: 837 837
Size: 4901
DrdI GACnn_nn'nnGTC
Cuts at: 2607 3465 2607
Size: 858 4043
EaeI y'GGCC_r
Cuts at: 1201 2486 2660 3051 3078 4423 1201
Size: 1285 174 391 27 1345 1679
Fragments arranged by size:
1679 1345 1285 391 174 27
EagI C'GGCC_G
Cuts at: 2486 2486
Size: 4901
EarI CTCTTCn'nnn_
Cuts at: 777 886 2924 3134 777
Size: 109 2038 210 2544
Fragments arranged by size:
2544 2038 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3109 3419 3565 4711 3109
Size: 310 146 1146 3299
Fragments arranged by size:
3299 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1913 1913
Size: 4901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1022 2726 3158 3905 4173 932
Size: 90 1704 432 747 268 1660
Fragments arranged by size:
1704 1660 747 432 268 90
EcoO109I rG'GnC_Cy
Cuts at: 2040 2040
Size: 4901
EcoRI G'AATT_C
Cuts at: 1435 1905 1435
Size: 470 4431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4901
FspI TGC'GCA
Cuts at: 2682 2682
Size: 4901
HaeII r_GCGC'y
Cuts at: 695 846 1247 2335 2583 3605 4584 695
Size: 151 401 1088 248 1022 979 1012
Fragments arranged by size:
1088 1022 1012 979 401 248 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1562 1594 2743 2773 2775 2805 1562
Size: 32 1149 30 2 30 3658
Fragments arranged by size:
3658 1149 32 30 30 2
HincII GTy'rAC
Cuts at: 1940 1940
Size: 4901
KasI G'GCGC_C
Cuts at: 2331 2579 2331
Size: 248 4653
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4901
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3572 3756 3572
Size: 184 4717
MscI TGG'CCA
Cuts at: 2662 2662
Size: 4901
MslI CAynn'nnrTG
Cuts at: 650 739 878 1318 1727 2890 3017 650
Size: 89 139 440 409 1163 127 2534
Fragments arranged by size:
2534 1163 440 409 139 127 89
MspA1I CmG'CkG
Cuts at: 1147 2012 2326 2686 3699 3944 1147
Size: 865 314 360 1013 245 2104
Fragments arranged by size:
2104 1013 865 360 314 245
NaeI GCC'GGC
Cuts at: 3082 3082
Size: 4901
NarI GG'CG_CC
Cuts at: 2332 2580 2332
Size: 248 4653
NcoI C'CATG_G
Cuts at: 1722 1722
Size: 4901
NgoMIV G'CCGG_C
Cuts at: 3080 3080
Size: 4901
NheI G'CTAG_C
Cuts at: 765 765
Size: 4901
NspI r_CATG'y
Cuts at: 186 589 649 1086 1606 1758 1879 1954 2454
Size: 403 60 437 520 152 121 75 500
Cuts at: 2454 3361 186
Size: 907 1726
Fragments arranged by size:
1726 907 520 500 437 403 152 121
75 60
PasI CC'CwG_GG
Cuts at: 792 792
Size: 4901
PciI A'CATG_T
Cuts at: 1754 3357 1754
Size: 1603 3298
PflMI CCAn_nnn'nTGG
Cuts at: 931 1111 931
Size: 180 4721
PfoI T'CCnGG_A
Cuts at: 1760 1760
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4869
PshAI GACnn'nnGTC
Cuts at: 920 920
Size: 4901
PstI C_TGCA'G
Cuts at: 164 708 1146 1948 2633 164
Size: 544 438 802 685 2432
Fragments arranged by size:
2432 802 685 544 438
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 1147 2326 2686 1147
Size: 1179 360 3362
Fragments arranged by size:
3362 1179 360
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 1915 1915
Size: 4901
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4901
SapI GCTCTTCn'nnn_
Cuts at: 2924 3134 2924
Size: 210 4691
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4901
ScaI AGT'ACT
Cuts at: 4418 4418
Size: 4901
SexAI A'CCwGG_T
Cuts at: 1379 1379
Size: 4901
SfcI C'TryA_G
Cuts at: 33 117 160 704 1142 1160 1944 2629 3622
Size: 84 43 544 438 18 784 685 993
Cuts at: 3622 3813 33
Size: 191 1121
Fragments arranged by size:
1121 993 784 685 544 438 191 84
43 18
SfoI GGC'GCC
Cuts at: 2333 2581 2333
Size: 248 4653
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4901
SmlI C'TyrA_G
Cuts at: 2056 2971 3463 3725 2056
Size: 915 492 262 3232
Fragments arranged by size:
3232 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4901
SphI G_CATG'C
Cuts at: 649 1086 1954 2454 649
Size: 437 868 500 3096
Fragments arranged by size:
3096 868 500 437
StyI C'CwwG_G
Cuts at: 145 925 1209 1566 1722 2035 2389 145
Size: 780 284 357 156 313 354 2657
Fragments arranged by size:
2657 780 357 354 313 284 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 384 1442 2484 2617 3076 3285 384
Size: 1058 1042 133 459 209 2000
Fragments arranged by size:
2000 1058 1042 459 209 133
TatI w'GTAC_w
Cuts at: 325 4416 325
Size: 4091 810
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 4869
Tth111I GACn'n_nGTC
Cuts at: 2698 2698
Size: 4901
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 1281 2396 471
Size: 810 1115 2976
Fragments arranged by size:
2976 1115 810
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4901
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BpmI BsaXI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI