Restriction map for
pF1KB4637
(Circular) (Six-base) MAPSORT of: pF1KB4637.seq Check: 1481 from: 1 to: 4013
pF1KB4637 4013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 392 714 392
Size: 322 3691
AccI GT'mk_AC
Cuts at: 1051 1051
Size: 4013
Acc65I G'GTAC_C
Cuts at: 295 1029 295
Size: 734 3279
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 547 710 1838 2270 3017 3285 475
Size: 72 163 1128 432 747 268 1203
Fragments arranged by size:
1203 1128 747 432 268 163 72
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AhdI GACnn_n'nnGTC
Cuts at: 339 339
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 364 1318 2885 364
Size: 954 1567 1492
Fragments arranged by size:
1567 1492 954
ApaLI G'TGCA_C
Cuts at: 2783 2783
Size: 4013
ApoI r'AATT_y
Cuts at: 196 1017 3366 3622 196
Size: 821 2349 256 587
Fragments arranged by size:
2349 821 587 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 612 636 1033 612
Size: 24 397 3592
Fragments arranged by size:
3592 397 24
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4013
BanI G'GyrC_C
Cuts at: 295 1029 1443 1691 1726 295
Size: 734 414 248 35 2582
Fragments arranged by size:
2582 734 414 248 35
BanII G_rGCy'C
Cuts at: 988 1027 2057 988
Size: 39 1030 2944
Fragments arranged by size:
2944 1030 39
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BbsI GAAGACnn'nnnn_
Cuts at: 191 191
Size: 4013
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 666 1533 666
Size: 867 3146
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 353 1638 1731 2787 353
Size: 1285 93 1056 1579
Fragments arranged by size:
1579 1285 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 152 410 1225 1306 1641 3785 152
Size: 258 815 81 335 2144 380
Fragments arranged by size:
2144 815 380 335 258 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 2380 168
Size: 2212 1801
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 550 3717 550
Size: 3167 846
BsaAI yAC'GTr
Cuts at: 552 1366 552
Size: 814 3199
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 896 1202 1723 2401 2675 2822 3233 896
Size: 306 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 306 274 147
BseYI C'CCAG_C
Cuts at: 271 314 598 617 1438 2196 2773 3269 271
Size: 43 284 19 821 758 577 496 1015
Fragments arranged by size:
1015 821 758 577 496 284 43 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 186 186
Size: 4013
BsiEI CG_ry'CG
Cuts at: 104 837 1555 1601 2809 104
Size: 733 718 46 1208 1308
Fragments arranged by size:
1308 1208 733 718 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2787 1027
Size: 778 190 792 2253
Fragments arranged by size:
2253 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 353 988 1027 1638 1731 1805 1995 2057 2787
Size: 635 39 611 93 74 190 62 730
Cuts at: 2787 353
Size: 1579
Fragments arranged by size:
1579 730 635 611 190 93 74 62
39
BspEI T'CCGG_A
Cuts at: 896 3233 896
Size: 2337 1676
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 714 817 1063 1277 1579 1960 392
Size: 322 103 246 214 302 381 2445
Fragments arranged by size:
2445 381 322 302 246 214 103
BsrBI CCG'CTC
Cuts at: 50 974 2305 2359 3843 4008 50
Size: 924 1331 54 1484 165 55
Fragments arranged by size:
1484 1331 924 165 55 54
BsrDI GCAATG_nn'
Cuts at: 762 1925 762
Size: 1163 2850
BsrFI r'CCGG_y
Cuts at: 832 2011 2192 2401 832
Size: 1179 181 209 2444
Fragments arranged by size:
2444 1179 209 181
BssSI C'ACGA_G
Cuts at: 564 802 2284 2642 564
Size: 238 1482 358 1935
Fragments arranged by size:
1935 1482 358 238
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 2374 2374
Size: 4013
BstYI r'GATC_y
Cuts at: 1 643 1038 1505 1528 1863 2109 3121 3207
Size: 642 395 467 23 335 246 1012 86
Cuts at: 3207 3219 3565 1
Size: 12 346 449
Fragments arranged by size:
1012 642 467 449 395 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 179 107
Size: 72 3941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 321 1963 2144 2307 321
Size: 1642 181 163 2027
Fragments arranged by size:
2027 1642 181 163
BtsI GCAGTG_nn'
Cuts at: 287 1256 287
Size: 969 3044
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 834 1598 1772 2163 2190 3535 834
Size: 764 174 391 27 1345 1312
Fragments arranged by size:
1345 1312 764 391 174 27
EagI C'GGCC_G
Cuts at: 834 1598 834
Size: 764 3249
EarI CTCTTCn'nnn_
Cuts at: 463 2036 2246 463
Size: 1573 210 2230
Fragments arranged by size:
2230 1573 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 949 2221 2531 2677 3823 949
Size: 1272 310 146 1146 1139
Fragments arranged by size:
1272 1146 1139 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 547 710 1838 2270 3017 3285 475
Size: 72 163 1128 432 747 268 1203
Fragments arranged by size:
1203 1128 747 432 268 163 72
EcoO109I rG'GnC_Cy
Cuts at: 798 1152 798
Size: 354 3659
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
EcoRV GAT'ATC
Cuts at: 585 585
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 1447 1695 2717 3696 1447
Size: 248 1022 979 1764
Fragments arranged by size:
1764 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 325 357 595 619 627 651 1855
Size: 32 190 32 238 24 8 24 1204
Cuts at: 1855 1885 1887 1917 103
Size: 30 2 30 2199
Fragments arranged by size:
2199 1204 238 190 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 299 1033 299
Size: 734 3279
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 2684 2868 992
Size: 1692 184 2137
Fragments arranged by size:
2137 1692 184
MscI TGG'CCA
Cuts at: 1774 1774
Size: 4013
MslI CAynn'nnrTG
Cuts at: 772 2002 2129 772
Size: 1230 127 2656
Fragments arranged by size:
2656 1230 127
MspA1I CmG'CkG
Cuts at: 371 541 1124 1438 1798 2811 3056 371
Size: 170 583 314 360 1013 245 1328
Fragments arranged by size:
1328 1013 583 360 314 245 170
NaeI GCC'GGC
Cuts at: 834 2194 834
Size: 1360 2653
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NcoI C'CATG_G
Cuts at: 107 107
Size: 4013
NgoMIV G'CCGG_C
Cuts at: 832 2192 832
Size: 1360 2653
NspI r_CATG'y
Cuts at: 1066 1566 2473 1066
Size: 500 907 2606
Fragments arranged by size:
2606 907 500
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PfoI T'CCnGG_A
Cuts at: 988 1001 988
Size: 13 4000
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 4013
PshAI GACnn'nnGTC
Cuts at: 706 706
Size: 4013
PspXI vC'TCGA_Gb
Cuts at: 636 636
Size: 4013
PstI C_TGCA'G
Cuts at: 389 1060 1745 389
Size: 671 685 2657
Fragments arranged by size:
2657 685 671
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 385 1056 1741 2734 2925 33
Size: 352 671 685 993 191 1121
Fragments arranged by size:
1121 993 685 671 352 191
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4013
SmlI C'TyrA_G
Cuts at: 636 1168 2083 2575 2837 636
Size: 532 915 492 262 1812
Fragments arranged by size:
1812 915 532 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 107 1147 1501 107
Size: 1040 354 2619
Fragments arranged by size:
2619 1040 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 1729 2188 2397 1596
Size: 133 459 209 3212
Fragments arranged by size:
3212 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 710 742 3530 3562 710
Size: 32 2788 32 1161
Fragments arranged by size:
2788 1161 32 32
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 1508
Size: 4013
XhoI C'TCGA_G
Cuts at: 636 636
Size: 4013
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4013
XmnI GAAnn'nnTTC
Cuts at: 997 997
Size: 4013
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PshAI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI