Restriction map for
pF1KB4640
(Circular) (Six-base) MAPSORT of: pF1KB4640.seq Check: 7744 from: 1 to: 4955
pF1KB4640 4955 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1993 1993
Size: 4955
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 1225 1423 1670 2780 3212 3565 4227 524
Size: 701 198 247 1110 432 353 662 1252
Fragments arranged by size:
1252 1110 701 662 432 353 247 198
AflII C'TTAA_G
Cuts at: 496 496
Size: 4955
AflIII A'CryG_T
Cuts at: 2307 4107 4388 2307
Size: 1800 281 2874
Fragments arranged by size:
2874 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1829 1861 1829
Size: 32 4923
AlwNI CAG_nnn'CTG
Cuts at: 836 2260 3698 836
Size: 1424 1438 2093
Fragments arranged by size:
2093 1438 1424
ApaI G_GGCC'C
Cuts at: 638 638
Size: 4955
ApaLI G'TGCA_C
Cuts at: 3793 3793
Size: 4955
ApoI r'AATT_y
Cuts at: 590 1959 4308 4564 590
Size: 1369 2349 256 981
Fragments arranged by size:
2349 1369 981 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 1975 1975
Size: 4955
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1131 1164 1131
Size: 33 4922
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 1971 2385 2633 2668 1971
Size: 414 248 35 4258
Fragments arranged by size:
4258 414 248 35
BanII G_rGCy'C
Cuts at: 638 1334 1969 2999 638
Size: 696 635 1030 2594
Fragments arranged by size:
2594 1030 696 635
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BbsI GAAGACnn'nnnn_
Cuts at: 1658 1781 1658
Size: 123 4832
BbvCI CC'TCA_GC
Cuts at: 1753 1753
Size: 4955
BciVI GTATCCnnnnn_n'
Cuts at: 2853 3909 4955 2853
Size: 1056 1046 2853
Fragments arranged by size:
2853 1056 1046
BclI T'GATC_A
Cuts at: 990 2475 990
Size: 1485 3470
BglII A'GATC_T
Cuts at: 444 2470 4507 444
Size: 2026 2037 892
Fragments arranged by size:
2037 2026 892
BlpI GC'TnA_GC
Cuts at: 374 2067 374
Size: 1693 3262
Bme1580I G_kGCm'C
Cuts at: 638 2580 2673 3797 638
Size: 1942 93 1124 1796
Fragments arranged by size:
1942 1796 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 490 1731 2167 2248 2583 4727 490
Size: 1241 436 81 335 2144 718
Fragments arranged by size:
2144 1241 718 436 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 4955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 445 493 664 1790 123
Size: 322 48 171 1126 3288
Fragments arranged by size:
3288 1126 322 171 48
Bpu10I CC'TnA_GC
Cuts at: 596 1451 1753 596
Size: 855 302 3798
Fragments arranged by size:
3798 855 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 2131 3010 3724 4022 877
Size: 1254 879 714 298 1810
Fragments arranged by size:
1810 1254 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 578 4659 578
Size: 4081 874
BsaAI yAC'GTr
Cuts at: 2308 2308
Size: 4955
BsaBI GATnn'nnATC
Cuts at: 6 2469 4506 6
Size: 2463 2037 455
Fragments arranged by size:
2463 2037 455
BsaHI Gr'CG_yC
Cuts at: 2386 2634 3336 4832 2386
Size: 248 702 1496 2509
Fragments arranged by size:
2509 1496 702 248
BsaWI w'CCGG_w
Cuts at: 688 2144 2665 3343 3754 3901 4175 688
Size: 1456 521 678 411 147 274 1468
Fragments arranged by size:
1468 1456 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 1372
Size: 30 4925
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 880 1324 1928 367
Size: 513 444 604 3394
Fragments arranged by size:
3394 604 513 444
BseYI C'CCAG_C
Cuts at: 766 1252 2380 3138 3803 4211 766
Size: 486 1128 758 665 408 1510
Fragments arranged by size:
1510 1128 758 665 486 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1320 1338 1320
Size: 18 4937
BsiEI CG_ry'CG
Cuts at: 104 2497 2543 3773 104
Size: 2393 46 1230 1286
Fragments arranged by size:
2393 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 602 1969 2747 2937 3797 602
Size: 1367 778 190 860 1760
Fragments arranged by size:
1760 1367 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 602 638 1334 1969 2580 2673 2747 2937 2999
Size: 36 696 635 611 93 74 190 62
Cuts at: 2999 3797 602
Size: 798 1760
Fragments arranged by size:
1760 798 696 635 611 190 93 74
62 36
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 744 2005 2219 2521 2902 744
Size: 1261 214 302 381 2797
Fragments arranged by size:
2797 1261 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3247 3301 4785 4950 50
Size: 3197 54 1484 165 55
Fragments arranged by size:
3197 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 2867 1541
Size: 1326 3629
BsrFI r'CCGG_y
Cuts at: 2953 3134 2953
Size: 181 4774
BssSI C'ACGA_G
Cuts at: 3226 3934 3226
Size: 708 4247
BstAPI GCAn_nnn'nTGC
Cuts at: 2332 2332
Size: 4955
BstBI TT'CG_AA
Cuts at: 1791 3316 1791
Size: 1525 3430
BstXI CCAn_nnnn'nTGG
Cuts at: 960 1179 960
Size: 219 4736
BstYI r'GATC_y
Cuts at: 1 444 1980 2447 2470 2805 3051 3357 3369
Size: 443 1536 467 23 335 246 306 12
Cuts at: 3369 3455 4507 1
Size: 86 1052 449
Fragments arranged by size:
1536 1052 467 449 443 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2905 3086 3249 2905
Size: 181 163 4611
Fragments arranged by size:
4611 181 163
BtsI GCAGTG_nn'
Cuts at: 2198 2198
Size: 4955
DraI TTT'AAA
Cuts at: 1677 1954 1677
Size: 277 4678
DrdI GACnn_nn'nnGTC
Cuts at: 2661 4005 2661
Size: 1344 3611
EaeI y'GGCC_r
Cuts at: 2540 2714 3105 3132 4477 2540
Size: 174 391 27 1345 3018
Fragments arranged by size:
3018 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2540 2540
Size: 4955
EarI CTCTTCn'nnn_
Cuts at: 643 2978 3188 643
Size: 2335 210 2410
Fragments arranged by size:
2410 2335 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3163 3905 4051 4765 3163
Size: 742 146 714 3353
Fragments arranged by size:
3353 742 714 146
EcoICRI GAG'CTC
Cuts at: 1967 1967
Size: 4955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 445 493 524 664 1225 1423 1670 1790
Size: 322 48 31 140 561 198 247 120
Cuts at: 1790 2780 3212 3565 4227 123
Size: 990 432 353 662 851
Fragments arranged by size:
990 851 662 561 432 353 322 247
198 140 120 48 31
EcoO109I rG'GnC_Cy
Cuts at: 635 1293 1607 1725 2094 635
Size: 658 314 118 369 3496
Fragments arranged by size:
3496 658 369 314 118
EcoRI G'AATT_C
Cuts at: 1959 1959
Size: 4955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 822 1831 2974 822
Size: 1009 1143 2803
Fragments arranged by size:
2803 1143 1009
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 2389 2637 3867 4638 2389
Size: 248 1230 771 2706
Fragments arranged by size:
2706 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 644 664 676 696 2797 2827 2829 2859 644
Size: 20 12 20 2101 30 2 30 2740
Fragments arranged by size:
2740 2101 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1994 1994
Size: 4955
HindIII A'AGCT_T
Cuts at: 1341 1341
Size: 4955
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MfeI C'AATT_G
Cuts at: 405 405
Size: 4955
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 951 1571 3714 3898 951
Size: 620 2143 184 2008
Fragments arranged by size:
2143 2008 620 184
MscI TGG'CCA
Cuts at: 2716 2716
Size: 4955
MslI CAynn'nnrTG
Cuts at: 958 2944 3071 958
Size: 1986 127 2842
Fragments arranged by size:
2842 1986 127
MspA1I CmG'CkG
Cuts at: 1717 2066 2380 2740 3524 3769 1717
Size: 349 314 360 784 245 2903
Fragments arranged by size:
2903 784 360 349 314 245
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 4955
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 4955
NspI r_CATG'y
Cuts at: 2008 2508 4111 2008
Size: 500 1603 2852
Fragments arranged by size:
2852 1603 500
PciI A'CATG_T
Cuts at: 4107 4107
Size: 4955
PfoI T'CCnGG_A
Cuts at: 641 641
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 4923
PpuMI rG'GwC_Cy
Cuts at: 1293 1725 1293
Size: 432 4523
PspOMI G'GGCC_C
Cuts at: 634 634
Size: 4955
PstI C_TGCA'G
Cuts at: 1875 2002 2687 1875
Size: 127 685 4143
Fragments arranged by size:
4143 685 127
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 1717 2380 2740 1717
Size: 663 360 3932
Fragments arranged by size:
3932 663 360
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1969 1969
Size: 4955
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 2978 3188 2978
Size: 210 4745
SbfI CC_TGCA'GG
Cuts at: 2002 2002
Size: 4955
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SfcI C'TryA_G
Cuts at: 33 1828 1871 1942 1998 2683 3651 3842 33
Size: 1795 43 71 56 685 968 191 1146
Fragments arranged by size:
1795 1146 968 685 191 71 56 43
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 1977 1977
Size: 4955
SmlI C'TyrA_G
Cuts at: 496 892 2110 3025 3739 4001 496
Size: 396 1218 915 714 262 1450
Fragments arranged by size:
1450 1218 915 714 396 262
SpeI A'CTAG_T
Cuts at: 67 940 67
Size: 873 4082
SphI G_CATG'C
Cuts at: 2008 2508 2008
Size: 500 4455
SspI AAT'ATT
Cuts at: 630 630
Size: 4955
StyI C'CwwG_G
Cuts at: 723 806 1631 2089 2443 723
Size: 83 825 458 354 3235
Fragments arranged by size:
3235 825 458 354 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2538 2671 3130 3339 2538
Size: 133 459 209 4154
Fragments arranged by size:
4154 459 209 133
TatI w'GTAC_w
Cuts at: 4470 4470
Size: 4955
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 4923
Tth111I GACn'n_nGTC
Cuts at: 1723 2752 1723
Size: 1029 3926
XbaI T'CTAG_A
Cuts at: 1546 1986 1546
Size: 440 4515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 127 1254 1765 2450 127
Size: 1127 511 685 2632
Fragments arranged by size:
2632 1127 685 511
XmaI C'CCGG_G
Cuts at: 1975 1975
Size: 4955
XmnI GAAnn'nnTTC
Cuts at: 115 115
Size: 4955
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AscI
AvrII BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI