Restriction map for
pF1KB4653
(Circular) (Six-base) MAPSORT of: pF1KB4653.seq Check: 3728 from: 1 to: 3713
pF1KB4653 3713 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 729 729
Size: 3713
AclI AA'CG_TT
Cuts at: 3553 3553
Size: 3713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 1538 1970 2717 2985 629
Size: 909 432 747 268 1357
Fragments arranged by size:
1357 909 747 432 268
AflIII A'CryG_T
Cuts at: 1065 2169 3146 1065
Size: 1104 977 1632
Fragments arranged by size:
1632 1104 977
AgeI A'CCGG_T
Cuts at: 2101 2101
Size: 3713
AlwNI CAG_nnn'CTG
Cuts at: 1018 2585 1018
Size: 1567 2146
ApaLI G'TGCA_C
Cuts at: 2483 2483
Size: 3713
ApoI r'AATT_y
Cuts at: 717 3066 3322 717
Size: 2349 256 1108
Fragments arranged by size:
2349 1108 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 733 733
Size: 3713
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 729 1143 1391 1426 729
Size: 414 248 35 3016
Fragments arranged by size:
3016 414 248 35
BanII G_rGCy'C
Cuts at: 644 727 1757 644
Size: 83 1030 2600
Fragments arranged by size:
2600 1030 83
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BbsI GAAGACnn'nnnn_
Cuts at: 124 178 124
Size: 54 3659
BciVI GTATCCnnnnn_n'
Cuts at: 291 1611 2372 3713 291
Size: 1320 761 1341 291
Fragments arranged by size:
1341 1320 761 291
BclI T'GATC_A
Cuts at: 1233 1233
Size: 3713
BglII A'GATC_T
Cuts at: 1228 3265 1228
Size: 2037 1676
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 1338 1431 2487 1338
Size: 93 1056 2564
Fragments arranged by size:
2564 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 925 1006 1341 3485 925
Size: 81 335 2144 1153
Fragments arranged by size:
2144 1153 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 3713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1768 2260 2558 889
Size: 879 492 298 2044
Fragments arranged by size:
2044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3417 3417
Size: 3713
BsaAI yAC'GTr
Cuts at: 1066 1066
Size: 3713
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 1144 1392 2094 3590 1144
Size: 248 702 1496 1267
Fragments arranged by size:
1496 1267 702 248
BsaWI w'CCGG_w
Cuts at: 160 902 1423 2101 2375 2522 2933 160
Size: 742 521 678 274 147 411 940
Fragments arranged by size:
940 742 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 665 695 665
Size: 30 3683
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 689 689
Size: 3713
BseYI C'CCAG_C
Cuts at: 1138 1896 2473 2969 1138
Size: 758 577 496 1882
Fragments arranged by size:
1882 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1255 1301 2509 104
Size: 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 46
BsiHKAI G_wGCw'C
Cuts at: 727 1505 1695 2487 727
Size: 778 190 792 1953
Fragments arranged by size:
1953 792 778 190
BsiWI C'GTAC_G
Cuts at: 122 122
Size: 3713
Bsp1286I G_dGCh'C
Cuts at: 644 727 1338 1431 1505 1695 1757 2487 644
Size: 83 611 93 74 190 62 730 1870
Fragments arranged by size:
1870 730 611 190 93 83 74 62
BspEI T'CCGG_A
Cuts at: 160 2933 160
Size: 2773 940
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 137 184 763 977 1279 1660 137
Size: 47 579 214 302 381 2190
Fragments arranged by size:
2190 579 381 302 214 47
BsrBI CCG'CTC
Cuts at: 50 2005 2059 3543 3708 50
Size: 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 359 1625 359
Size: 1266 2447
BsrFI r'CCGG_y
Cuts at: 1711 1892 2101 1711
Size: 181 209 3323
Fragments arranged by size:
3323 209 181
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 704 1090 704
Size: 386 3327
BstBI TT'CG_AA
Cuts at: 621 2074 621
Size: 1453 2260
BstYI r'GATC_y
Cuts at: 1 738 1205 1228 1563 1809 2821 2907 2919
Size: 737 467 23 335 246 1012 86 12
Cuts at: 2919 3265 1
Size: 346 449
Fragments arranged by size:
1012 737 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1663 1844 2007 1663
Size: 181 163 3369
Fragments arranged by size:
3369 181 163
BtsI GCAGTG_nn'
Cuts at: 675 956 675
Size: 281 3432
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 1419 2277 1419
Size: 858 2855
EaeI y'GGCC_r
Cuts at: 1298 1472 1863 1890 3235 1298
Size: 174 391 27 1345 1776
Fragments arranged by size:
1776 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1298 1298
Size: 3713
EarI CTCTTCn'nnn_
Cuts at: 688 1736 1946 688
Size: 1048 210 2455
Fragments arranged by size:
2455 1048 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 725
Size: 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 1538 1970 2717 2985 629
Size: 909 432 747 268 1357
Fragments arranged by size:
1357 909 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 852 852
Size: 3713
EcoRI G'AATT_C
Cuts at: 717 717
Size: 3713
EcoRV GAT'ATC
Cuts at: 615 663 615
Size: 48 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 634 1732 634
Size: 1098 2615
FspI TGC'GCA
Cuts at: 1494 1494
Size: 3713
HaeII r_GCGC'y
Cuts at: 1147 1395 2417 3396 1147
Size: 248 1022 979 1464
Fragments arranged by size:
1464 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1555 1585 1587 1617 1555
Size: 30 2 30 3651
Fragments arranged by size:
3651 30 30 2
HincII GTy'rAC
Cuts at: 752 752
Size: 3713
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 733 733
Size: 3713
MluI A'CGCG_T
Cuts at: 3146 3146
Size: 3713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2568 2384
Size: 184 3529
MscI TGG'CCA
Cuts at: 1474 1474
Size: 3713
MslI CAynn'nnrTG
Cuts at: 440 1702 1829 440
Size: 1262 127 2324
Fragments arranged by size:
2324 1262 127
MspA1I CmG'CkG
Cuts at: 824 1138 1498 2511 2756 824
Size: 314 360 1013 245 1781
Fragments arranged by size:
1781 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1894 1894
Size: 3713
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 107
Size: 3713
NgoMIV G'CCGG_C
Cuts at: 1892 1892
Size: 3713
NspI r_CATG'y
Cuts at: 439 766 1266 2173 439
Size: 327 500 907 1979
Fragments arranged by size:
1979 907 500 327
PciI A'CATG_T
Cuts at: 2169 2169
Size: 3713
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PshAI GACnn'nnGTC
Cuts at: 178 178
Size: 3713
PstI C_TGCA'G
Cuts at: 760 1445 760
Size: 685 3028
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 1138 1498 1138
Size: 360 3353
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 727 727
Size: 3713
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 688 1736 1946 688
Size: 1048 210 2455
Fragments arranged by size:
2455 1048 210
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 3230 3230
Size: 3713
SfcI C'TryA_G
Cuts at: 33 286 342 756 1441 2434 2625 33
Size: 253 56 414 685 993 191 1121
Fragments arranged by size:
1121 993 685 414 253 191 56
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 735 735
Size: 3713
SmlI C'TyrA_G
Cuts at: 868 1783 2275 2537 868
Size: 915 492 262 2044
Fragments arranged by size:
2044 915 492 262
SpeI A'CTAG_T
Cuts at: 67 559 67
Size: 492 3221
SphI G_CATG'C
Cuts at: 439 766 1266 439
Size: 327 500 2886
Fragments arranged by size:
2886 500 327
StyI C'CwwG_G
Cuts at: 107 847 1201 107
Size: 740 354 2619
Fragments arranged by size:
2619 740 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1296 1429 1888 2097 1296
Size: 133 459 209 2912
Fragments arranged by size:
2912 459 209 133
TatI w'GTAC_w
Cuts at: 3228 3228
Size: 3713
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 665 697 3230 3262 665
Size: 32 2533 32 1116
Fragments arranged by size:
2533 1116 32 32
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 1208 447
Size: 761 2952
XmaI C'CCGG_G
Cuts at: 733 733
Size: 3713
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsgI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI