Restriction map for
pF1KB4658
(Circular) (Six-base) MAPSORT of: pF1KB4658.seq Check: 6943 from: 1 to: 5531
pF1KB4658 5531 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1553 1553
Size: 5531
AatII G_ACGT'C
Cuts at: 1838 1838
Size: 5531
AccI GT'mk_AC
Cuts at: 2468 2569 2468
Size: 101 5430
Acc65I G'GTAC_C
Cuts at: 2547 2547
Size: 5531
AclI AA'CG_TT
Cuts at: 1955 5371 1955
Size: 3416 2115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 1819 2364 3356 3788 4141 4803 123
Size: 1696 545 992 432 353 662 851
Fragments arranged by size:
1696 992 851 662 545 432 353
AflIII A'CryG_T
Cuts at: 2883 4683 4964 2883
Size: 1800 281 3450
Fragments arranged by size:
3450 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 589 1403 589
Size: 814 4717
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1735 1767 1735
Size: 32 5499
AlwNI CAG_nnn'CTG
Cuts at: 411 435 711 739 1927 2130 2514 2836 4274
Size: 24 276 28 1188 203 384 322 1438
Cuts at: 4274 411
Size: 1668
Fragments arranged by size:
1668 1438 1188 384 322 276 203 28
24
ApaI G_GGCC'C
Cuts at: 859 1488 1784 859
Size: 629 296 4606
Fragments arranged by size:
4606 629 296
ApaLI G'TGCA_C
Cuts at: 4369 4369
Size: 5531
ApoI r'AATT_y
Cuts at: 354 2535 4884 5140 354
Size: 2181 2349 256 745
Fragments arranged by size:
2349 2181 745 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
AvaI C'yCGr_G
Cuts at: 207 1617 2551 207
Size: 1410 934 3187
Fragments arranged by size:
3187 1410 934
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 507 540 507
Size: 33 5498
BamHI G'GATC_C
Cuts at: 497 2316 2556 497
Size: 1819 240 3472
Fragments arranged by size:
3472 1819 240
BanI G'GyrC_C
Cuts at: 1657 2547 2961 3209 3244 1657
Size: 890 414 248 35 3944
Fragments arranged by size:
3944 890 414 248 35
BanII G_rGCy'C
Cuts at: 400 859 1387 1425 1488 1513 1762 1784 2545
Size: 459 528 38 63 25 249 22 761
Cuts at: 2545 3575 400
Size: 1030 2356
Fragments arranged by size:
2356 1030 761 528 459 249 63 38
25 22
BbeI G_GCGC'C
Cuts at: 2965 3213 2965
Size: 248 5283
BbsI GAAGACnn'nnnn_
Cuts at: 666 2223 666
Size: 1557 3974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1077 1111 90
Size: 34 953 34 4510
Fragments arranged by size:
4510 953 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3429 4485 5531 3429
Size: 1056 1046 3429
Fragments arranged by size:
3429 1056 1046
BclI T'GATC_A
Cuts at: 917 1202 3051 917
Size: 285 1849 3397
Fragments arranged by size:
3397 1849 285
BglI GCCn_nnn'nGGC
Cuts at: 481 863 481
Size: 382 5149
BglII A'GATC_T
Cuts at: 3046 5083 3046
Size: 2037 3494
BlpI GC'TnA_GC
Cuts at: 1424 1976 2643 1424
Size: 552 667 4312
Fragments arranged by size:
4312 667 552
Bme1580I G_kGCm'C
Cuts at: 859 1488 1660 1784 2346 3156 3249 4373 859
Size: 629 172 124 562 810 93 1124 2017
Fragments arranged by size:
2017 1124 810 629 562 172 124 93
BmrI ACTGGGnnnn_n'
Cuts at: 993 1656 2296 2743 2824 3159 5303 993
Size: 663 640 447 81 335 2144 1221
Fragments arranged by size:
2144 1221 663 640 447 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 419 1401 1632 3898 419
Size: 982 231 2266 2052
Fragments arranged by size:
2266 2052 982 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1688 1746 1807 1904 1955 349
Size: 1339 58 61 97 51 3925
Fragments arranged by size:
3925 1339 97 61 58 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2707 3586 4300 4598 2707
Size: 879 714 298 3640
Fragments arranged by size:
3640 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 661 2452 5235 661
Size: 1791 2783 957
Fragments arranged by size:
2783 1791 957
BsaAI yAC'GTr
Cuts at: 1074 2884 1074
Size: 1810 3721
BsaBI GATnn'nnATC
Cuts at: 6 919 3045 5082 6
Size: 913 2126 2037 455
Fragments arranged by size:
2126 2037 913 455
BsaHI Gr'CG_yC
Cuts at: 146 344 1835 2962 3210 3912 5408 146
Size: 198 1491 1127 248 702 1496 269
Fragments arranged by size:
1496 1491 1127 702 269 248 198
BsaWI w'CCGG_w
Cuts at: 1904 2720 3241 3919 4330 4477 4751 1904
Size: 816 521 678 411 147 274 2684
Fragments arranged by size:
2684 816 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 656 686 1217 1247 1553 1583 656
Size: 30 531 30 306 30 4604
Fragments arranged by size:
4604 531 306 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 571 1577 1580 1585 1804 2503 571
Size: 1006 3 5 219 699 3599
Fragments arranged by size:
3599 1006 699 219 5 3
BseYI C'CCAG_C
Cuts at: 374 460 795 2239 2393 2397 2956 3714 4379
Size: 86 335 1444 154 4 559 758 665
Cuts at: 4379 4787 374
Size: 408 1118
Fragments arranged by size:
1444 1118 758 665 559 408 335 154
86 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 549 559 643 763 1054 1153 1678 397
Size: 152 10 84 120 291 99 525 4250
Fragments arranged by size:
4250 525 291 152 120 99 84 10
BsiEI CG_ry'CG
Cuts at: 104 3073 3119 4349 104
Size: 2969 46 1230 1286
Fragments arranged by size:
2969 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 400 1154 1425 1820 2483 2545 3323 3513 4373
Size: 754 271 395 663 62 778 190 860
Cuts at: 4373 400
Size: 1558
Fragments arranged by size:
1558 860 778 754 663 395 271 190
62
Bsp1286I G_dGCh'C
Cuts at: 400 859 1154 1387 1425 1488 1513 1660 1762
Size: 459 295 233 38 63 25 147 102
Cuts at: 1762 1784 1820 2346 2483 2545 3156 3249 3323
Size: 22 36 526 137 62 611 93 74
Cuts at: 3323 3513 3575 4373 400
Size: 190 62 798 1558
Fragments arranged by size:
1558 798 611 526 459 295 233 190
147 137 102 93 74 63 62 62
38 36 25 22
BspEI T'CCGG_A
Cuts at: 1904 1904
Size: 5531
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 5531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1553 2581 2795 3097 3478 1553
Size: 1028 214 302 381 3606
Fragments arranged by size:
3606 1028 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3823 3877 5361 5526 50
Size: 3773 54 1484 165 55
Fragments arranged by size:
3773 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 840 3443 840
Size: 2603 2928
BsrFI r'CCGG_y
Cuts at: 1877 1884 3529 3710 1877
Size: 7 1645 181 3698
Fragments arranged by size:
3698 1645 181 7
BssSI C'ACGA_G
Cuts at: 3802 4510 3802
Size: 708 4823
BstAPI GCAn_nnn'nTGC
Cuts at: 2908 2908
Size: 5531
BstBI TT'CG_AA
Cuts at: 3892 3892
Size: 5531
BstXI CCAn_nnnn'nTGG
Cuts at: 2286 2286
Size: 5531
BstYI r'GATC_y
Cuts at: 1 497 605 941 1464 2108 2316 2556 3023
Size: 496 108 336 523 644 208 240 467
Cuts at: 3023 3046 3381 3627 3933 3945 4031 5083 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 644 523 496 467 449 336 335
306 246 240 208 108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 404 1963 404
Size: 1559 3972
BtgI C'CryG_G
Cuts at: 1052 1122 1143 1331 1362 1373 1706 1052
Size: 70 21 188 31 11 333 4877
Fragments arranged by size:
4877 333 188 70 31 21 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1584 3481 3662 3825 1584
Size: 1897 181 163 3290
Fragments arranged by size:
3290 1897 181 163
BtsI GCAGTG_nn'
Cuts at: 740 2774 740
Size: 2034 3497
DraI TTT'AAA
Cuts at: 2530 2530
Size: 5531
DraIII CAC_nnn'GTG
Cuts at: 307 307
Size: 5531
DrdI GACnn_nn'nnGTC
Cuts at: 2196 3237 4581 2196
Size: 1041 1344 3146
Fragments arranged by size:
3146 1344 1041
EaeI y'GGCC_r
Cuts at: 120 194 881 1175 1625 1685 2075 2258 3116
Size: 74 687 294 450 60 390 183 858
Cuts at: 3116 3290 3681 3708 5053 120
Size: 174 391 27 1345 598
Fragments arranged by size:
1345 858 687 598 450 391 390 294
183 174 74 60 27
EagI C'GGCC_G
Cuts at: 3116 3116
Size: 5531
EarI CTCTTCn'nnn_
Cuts at: 325 1906 3554 3764 325
Size: 1581 1648 210 2092
Fragments arranged by size:
2092 1648 1581 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3739 4481 4627 5341 3739
Size: 742 146 714 3929
Fragments arranged by size:
3929 742 714 146
EcoICRI GAG'CTC
Cuts at: 398 1423 2543 398
Size: 1025 1120 3386
Fragments arranged by size:
3386 1120 1025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 349 1688 1746 1807 1819 1904 1955 2364
Size: 226 1339 58 61 12 85 51 409
Cuts at: 2364 3356 3788 4141 4803 123
Size: 992 432 353 662 851
Fragments arranged by size:
1339 992 851 662 432 409 353 226
85 61 58 51 12
EcoNI CCTnn'n_nnAGG
Cuts at: 748 748
Size: 5531
EcoO109I rG'GnC_Cy
Cuts at: 408 423 856 1209 1368 1635 1766 1780 1781
Size: 15 433 353 159 267 131 14 1
Cuts at: 1781 2127 2198 2670 408
Size: 346 71 472 3269
Fragments arranged by size:
3269 472 433 353 346 267 159 131
71 15 14 1
EcoRI G'AATT_C
Cuts at: 2535 2535
Size: 5531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1329 3550 1329
Size: 2221 3310
FspI TGC'GCA
Cuts at: 1087 3312 1087
Size: 2225 3306
HaeII r_GCGC'y
Cuts at: 1396 2055 2965 3213 4443 5214 1396
Size: 659 910 248 1230 771 1713
Fragments arranged by size:
1713 1230 910 771 659 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 302 334 656 688 1302 1334 3373
Size: 32 80 32 322 32 614 32 2039
Cuts at: 3373 3403 3405 3435 190
Size: 30 2 30 2286
Fragments arranged by size:
2286 2039 614 322 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 288 1959 2445 2570 288
Size: 1671 486 125 3249
Fragments arranged by size:
3249 1671 486 125
HpaI GTT'AAC
Cuts at: 1959 1959
Size: 5531
KasI G'GCGC_C
Cuts at: 2961 3209 2961
Size: 248 5283
KpnI G_GTAC'C
Cuts at: 2551 2551
Size: 5531
MfeI C'AATT_G
Cuts at: 540 540
Size: 5531
MluI A'CGCG_T
Cuts at: 4964 4964
Size: 5531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 339 2064 4290 4474 314
Size: 25 1725 2226 184 1371
Fragments arranged by size:
2226 1725 1371 184 25
MscI TGG'CCA
Cuts at: 1177 1627 1687 2077 2260 3292 1177
Size: 450 60 390 183 1032 3416
Fragments arranged by size:
3416 1032 450 390 183 60
MslI CAynn'nnrTG
Cuts at: 640 925 1825 3520 3647 640
Size: 285 900 1695 127 2524
Fragments arranged by size:
2524 1695 900 285 127
MspA1I CmG'CkG
Cuts at: 237 327 378 464 533 777 795 1054 1145
Size: 90 51 86 69 244 18 259 91
Cuts at: 1145 1167 1375 1992 2018 2397 2511 2642 2956
Size: 22 208 617 26 379 114 131 314
Cuts at: 2956 3316 4100 4345 237
Size: 360 784 245 1423
Fragments arranged by size:
1423 784 617 379 360 314 259 245
244 208 131 114 91 90 86 69
51 26 22 18
NaeI GCC'GGC
Cuts at: 1886 3712 1886
Size: 1826 3705
NarI GG'CG_CC
Cuts at: 2962 3210 2962
Size: 248 5283
NcoI C'CATG_G
Cuts at: 1706 1706
Size: 5531
NgoMIV G'CCGG_C
Cuts at: 1884 3710 1884
Size: 1826 3705
NspI r_CATG'y
Cuts at: 1014 1719 1728 2584 3084 4687 1014
Size: 705 9 856 500 1603 1858
Fragments arranged by size:
1858 1603 856 705 500 9
PasI CC'CwG_GG
Cuts at: 2184 2249 2184
Size: 65 5466
PciI A'CATG_T
Cuts at: 4683 4683
Size: 5531
PfoI T'CCnGG_A
Cuts at: 1931 2057 1931
Size: 126 5405
PmeI GTTT'AAAC
Cuts at: 2530 2530
Size: 5531
PmlI CAC'GTG
Cuts at: 1074 1074
Size: 5531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1883 1915 3977 4009 1883
Size: 32 2062 32 3405
Fragments arranged by size:
3405 2062 32 32
PpuMI rG'GwC_Cy
Cuts at: 408 423 1368 1635 2127 2198 408
Size: 15 945 267 492 71 3741
Fragments arranged by size:
3741 945 492 267 71 15
PshAI GACnn'nnGTC
Cuts at: 1834 2464 1834
Size: 630 4901
PspOMI G'GGCC_C
Cuts at: 855 1484 1780 855
Size: 629 296 4606
Fragments arranged by size:
4606 629 296
PspXI vC'TCGA_Gb
Cuts at: 207 207
Size: 5531
PstI C_TGCA'G
Cuts at: 431 716 1392 2578 3263 431
Size: 285 676 1186 685 2699
Fragments arranged by size:
2699 1186 685 676 285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5531
PvuII CAG'CTG
Cuts at: 237 327 378 464 533 795 1992 2397 2511
Size: 90 51 86 69 262 1197 405 114
Cuts at: 2511 2956 3316 237
Size: 445 360 2452
Fragments arranged by size:
2452 1197 445 405 360 262 114 90
86 69 51
RsrII CG'GwC_CG
Cuts at: 3726 3726
Size: 5531
SacI G_AGCT'C
Cuts at: 400 1425 2545 400
Size: 1025 1120 3386
Fragments arranged by size:
3386 1120 1025
SacII CC_GC'GG
Cuts at: 1055 1146 1376 1055
Size: 91 230 5210
Fragments arranged by size:
5210 230 91
SalI G'TCGA_C
Cuts at: 2568 2568
Size: 5531
SanDI GG'GwC_CC
Cuts at: 1368 1368
Size: 5531
SapI GCTCTTCn'nnn_
Cuts at: 3554 3764 3554
Size: 210 5321
SbfI CC_TGCA'GG
Cuts at: 2578 2578
Size: 5531
ScaI AGT'ACT
Cuts at: 628 2377 5048 628
Size: 1749 2671 1111
Fragments arranged by size:
2671 1749 1111
SexAI A'CCwGG_T
Cuts at: 749 749
Size: 5531
SfcI C'TryA_G
Cuts at: 33 427 712 1388 1459 2574 3259 4227 4418
Size: 394 285 676 71 1115 685 968 191
Cuts at: 4418 33
Size: 1146
Fragments arranged by size:
1146 1115 968 685 676 394 285 191
71
SfoI GGC'GCC
Cuts at: 2963 3211 2963
Size: 248 5283
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
SmaI CCC'GGG
Cuts at: 2553 2553
Size: 5531
SmlI C'TyrA_G
Cuts at: 207 2686 3601 4315 4577 207
Size: 2479 915 714 262 1161
Fragments arranged by size:
2479 1161 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5531
SphI G_CATG'C
Cuts at: 1014 2584 3084 1014
Size: 1570 500 3461
Fragments arranged by size:
3461 1570 500
StuI AGG'CCT
Cuts at: 1577 1615 1968 1577
Size: 38 353 5140
Fragments arranged by size:
5140 353 38
StyI C'CwwG_G
Cuts at: 720 762 1631 1706 1847 2279 2665 3019 720
Size: 42 869 75 141 432 386 354 3232
Fragments arranged by size:
3232 869 432 386 354 141 75 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 230 454 1371 1638 2119 2476 3114 3247 3706
Size: 224 917 267 481 357 638 133 459
Cuts at: 3706 3915 230
Size: 209 1846
Fragments arranged by size:
1846 917 638 481 459 357 267 224
209 133
TatI w'GTAC_w
Cuts at: 626 1727 2117 2375 5046 626
Size: 1101 390 258 2671 1111
Fragments arranged by size:
2671 1111 1101 390 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1217 1249 1553 1585 1687 1719 5048 5080 1217
Size: 32 304 32 102 32 3329 32 1668
Fragments arranged by size:
3329 1668 304 102 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 277 1869 3328 277
Size: 1592 1459 2480
Fragments arranged by size:
2480 1592 1459
XbaI T'CTAG_A
Cuts at: 2562 2562
Size: 5531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 3026 468
Size: 2558 2973
XhoI C'TCGA_G
Cuts at: 207 207
Size: 5531
XmaI C'CCGG_G
Cuts at: 2551 2551
Size: 5531
ZraI GAC'GTC
Cuts at: 1836 1836
Size: 5531
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AscI AvrII BbvCI
BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII NdeI NheI NotI NruI NsiI PacI PflMI
PsiI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI