Restriction map for
pF1KB4659
(Circular) (Six-base) MAPSORT of: pF1KB4659.seq Check: 5936 from: 1 to: 4553
pF1KB4659 4553 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 943 943
Size: 4553
AccI GT'mk_AC
Cuts at: 335 1591 335
Size: 1256 3297
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 2378 2810 3557 3825 673
Size: 1705 432 747 268 1401
Fragments arranged by size:
1705 1401 747 432 268
AflIII A'CryG_T
Cuts at: 1905 3009 3986 1905
Size: 1104 977 2472
Fragments arranged by size:
2472 1104 977
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AleI CACnn'nnGTG
Cuts at: 1253 1267 1253
Size: 14 4539
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1189 1221 1189
Size: 32 4521
AlwNI CAG_nnn'CTG
Cuts at: 300 386 437 699 909 1194 1356 1858 3425
Size: 86 51 262 210 285 162 502 1567
Cuts at: 3425 300
Size: 1428
Fragments arranged by size:
1567 1428 502 285 262 210 162 86
51
ApaI G_GGCC'C
Cuts at: 190 239 564 1125 190
Size: 49 325 561 3618
Fragments arranged by size:
3618 561 325 49
ApaLI G'TGCA_C
Cuts at: 3323 3323
Size: 4553
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 165 183 230 289 565 1573 165
Size: 18 47 59 276 1008 3145
Fragments arranged by size:
3145 1008 276 59 47 18
BamHI G'GATC_C
Cuts at: 1578 1578
Size: 4553
BanI G'GyrC_C
Cuts at: 389 440 795 1104 1569 1983 2231 2266 389
Size: 51 355 309 465 414 248 35 2676
Fragments arranged by size:
2676 465 414 355 309 248 51 35
BanII G_rGCy'C
Cuts at: 143 190 219 239 564 689 1025 1101 1125
Size: 47 29 20 325 125 336 76 24
Cuts at: 1125 1567 2597 143
Size: 442 1030 2099
Fragments arranged by size:
2099 1030 442 336 325 125 76 47
29 24 20
BbeI G_GCGC'C
Cuts at: 393 444 1987 2235 393
Size: 51 1543 248 2711
Fragments arranged by size:
2711 1543 248 51
BbsI GAAGACnn'nnnn_
Cuts at: 661 1064 1148 1414 661
Size: 403 84 266 3800
Fragments arranged by size:
3800 403 266 84
BbvCI CC'TCA_GC
Cuts at: 1005 1005
Size: 4553
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglI GCCn_nnn'nGGC
Cuts at: 460 793 460
Size: 333 4220
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 190 239 564 1125 2178 2271 3327 190
Size: 49 325 561 1053 93 1056 1416
Fragments arranged by size:
1416 1056 1053 561 325 93 49
BmgBI CAC'GTC
Cuts at: 879 879
Size: 4553
BmrI ACTGGGnnnn_n'
Cuts at: 1765 1846 2181 4325 1765
Size: 81 335 2144 1993
Fragments arranged by size:
2144 1993 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 233 1115 2920 233
Size: 882 1805 1866
Fragments arranged by size:
1866 1805 882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 994 1039 1378 1458 1507 328
Size: 666 45 339 80 49 3374
Fragments arranged by size:
3374 666 339 80 49 45
Bpu10I CC'TnA_GC
Cuts at: 1005 1100 1005
Size: 95 4458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 1354 1729 2608 3100 3398 127
Size: 1227 375 879 492 298 1282
Fragments arranged by size:
1282 1227 879 492 375 298
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 1470 1906 1470
Size: 436 4117
BsaBI GATnn'nnATC
Cuts at: 6 2067 4104 6
Size: 2061 2037 455
Fragments arranged by size:
2061 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 390 441 940 1984 2232 2934 4430 173
Size: 217 51 499 1044 248 702 1496 296
Fragments arranged by size:
1496 1044 702 499 296 248 217 51
BsaWI w'CCGG_w
Cuts at: 1742 2263 2941 3215 3362 3773 1742
Size: 521 678 274 147 411 2522
Fragments arranged by size:
2522 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 712 742 712
Size: 30 4523
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 274 720 964 211
Size: 63 446 244 3800
Fragments arranged by size:
3800 446 244 63
BseYI C'CCAG_C
Cuts at: 238 546 1978 2736 3313 3809 238
Size: 308 1432 758 577 496 982
Fragments arranged by size:
1432 982 758 577 496 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 379 394 346
Size: 33 15 4505
Fragments arranged by size:
4505 33 15
BsiEI CG_ry'CG
Cuts at: 104 2095 2141 3349 104
Size: 1991 46 1208 1308
Fragments arranged by size:
1991 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 143 668 689 1274 1567 2345 2535 3327 143
Size: 525 21 585 293 778 190 792 1369
Fragments arranged by size:
1369 792 778 585 525 293 190 21
Bsp1286I G_dGCh'C
Cuts at: 143 190 219 239 564 668 689 1025 1101
Size: 47 29 20 325 104 21 336 76
Cuts at: 1101 1125 1274 1567 2178 2271 2345 2535 2597
Size: 24 149 293 611 93 74 190 62
Cuts at: 2597 3327 143
Size: 730 1369
Fragments arranged by size:
1369 730 611 336 325 293 190 149
104 93 76 74 62 47 29 24
21 20
BspEI T'CCGG_A
Cuts at: 3773 3773
Size: 4553
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 410 1603 1817 2119 2500 161
Size: 249 1193 214 302 381 2214
Fragments arranged by size:
2214 1193 381 302 249 214
BsrBI CCG'CTC
Cuts at: 50 1362 1507 2845 2899 4383 4548 50
Size: 1312 145 1338 54 1484 165 55
Fragments arranged by size:
1484 1338 1312 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 449 2465 449
Size: 2016 2537
BsrFI r'CCGG_y
Cuts at: 658 2551 2732 2941 658
Size: 1893 181 209 2270
Fragments arranged by size:
2270 1893 209 181
BsrGI T'GTAC_A
Cuts at: 1424 1424
Size: 4553
BssHII G'CGCG_C
Cuts at: 1492 1492
Size: 4553
BssSI C'ACGA_G
Cuts at: 2824 3182 2824
Size: 358 4195
BstAPI GCAn_nnn'nTGC
Cuts at: 402 909 1930 402
Size: 507 1021 3025
Fragments arranged by size:
3025 1021 507
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstEII G'GTnAC_C
Cuts at: 529 996 529
Size: 467 4086
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4553
BstYI r'GATC_y
Cuts at: 1 1578 2045 2068 2403 2649 3661 3747 3759
Size: 1577 467 23 335 246 1012 86 12
Cuts at: 3759 4105 1
Size: 346 449
Fragments arranged by size:
1577 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 761 1178 761
Size: 417 4136
BtgI C'CryG_G
Cuts at: 254 616 1146 254
Size: 362 530 3661
Fragments arranged by size:
3661 530 362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1224 2503 2684 2847 1224
Size: 1279 181 163 2930
Fragments arranged by size:
2930 1279 181 163
BtsI GCAGTG_nn'
Cuts at: 353 714 1299 1796 353
Size: 361 585 497 3110
Fragments arranged by size:
3110 585 497 361
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 428 506 613 680 2138 2312 2703 2730 4075
Size: 78 107 67 1458 174 391 27 1345
Cuts at: 4075 428
Size: 906
Fragments arranged by size:
1458 1345 906 391 174 107 78 67
27
EagI C'GGCC_G
Cuts at: 2138 2138
Size: 4553
EarI CTCTTCn'nnn_
Cuts at: 1438 2576 2786 1438
Size: 1138 210 3205
Fragments arranged by size:
3205 1138 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 1438 2761 3071 3217 4363 133
Size: 1305 1323 310 146 1146 323
Fragments arranged by size:
1323 1305 1146 323 310 146
EcoICRI GAG'CTC
Cuts at: 141 687 1565 141
Size: 546 878 3129
Fragments arranged by size:
3129 878 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 673 994 1039 1378 1458 1507 2378 2810
Size: 345 321 45 339 80 49 871 432
Cuts at: 2810 3557 3825 328
Size: 747 268 1056
Fragments arranged by size:
1056 871 747 432 345 339 321 268
80 49 45
EcoNI CCTnn'n_nnAGG
Cuts at: 271 760 271
Size: 489 4064
EcoO109I rG'GnC_Cy
Cuts at: 187 226 235 264 561 837 1692 187
Size: 39 9 29 297 276 855 3048
Fragments arranged by size:
3048 855 297 276 39 29 9
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 577 2334 577
Size: 1757 2796
HaeII r_GCGC'y
Cuts at: 245 393 444 889 1987 2235 3257 4236 245
Size: 148 51 445 1098 248 1022 979 562
Fragments arranged by size:
1098 1022 979 562 445 248 148 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 2395 2425 2427 2457 925
Size: 32 1438 30 2 30 3021
Fragments arranged by size:
3021 1438 32 30 30 2
HincII GTy'rAC
Cuts at: 1592 1592
Size: 4553
KasI G'GCGC_C
Cuts at: 389 440 1983 2231 389
Size: 51 1543 248 2711
Fragments arranged by size:
2711 1543 248 51
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3224 3408 3224
Size: 184 4369
MscI TGG'CCA
Cuts at: 430 2314 430
Size: 1884 2669
MslI CAynn'nnrTG
Cuts at: 112 253 416 1243 1253 1267 2542 2669 112
Size: 141 163 827 10 14 1275 127 1996
Fragments arranged by size:
1996 1275 827 163 141 127 14 10
MspA1I CmG'CkG
Cuts at: 909 1009 1167 1191 1664 1978 2338 3351 3596
Size: 100 158 24 473 314 360 1013 245
Cuts at: 3596 909
Size: 1866
Fragments arranged by size:
1866 1013 473 360 314 245 158 100
24
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 390 441 1984 2232 390
Size: 51 1543 248 2711
Fragments arranged by size:
2711 1543 248 51
NcoI C'CATG_G
Cuts at: 616 1146 616
Size: 530 4023
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NspI r_CATG'y
Cuts at: 903 1044 1500 1606 2106 3013 903
Size: 141 456 106 500 907 2443
Fragments arranged by size:
2443 907 500 456 141 106
PciI A'CATG_T
Cuts at: 3009 3009
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 386 437 1356 386
Size: 51 919 3583
Fragments arranged by size:
3583 919 51
PfoI T'CCnGG_A
Cuts at: 1016 1355 1484 1016
Size: 339 129 4085
Fragments arranged by size:
4085 339 129
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PmlI CAC'GTG
Cuts at: 1470 1470
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PpuMI rG'GwC_Cy
Cuts at: 264 837 264
Size: 573 3980
PspOMI G'GGCC_C
Cuts at: 186 235 560 1121 186
Size: 49 325 561 3618
Fragments arranged by size:
3618 561 325 49
PspXI vC'TCGA_Gb
Cuts at: 230 289 230
Size: 59 4494
PstI C_TGCA'G
Cuts at: 521 914 1600 2285 521
Size: 393 686 685 2789
Fragments arranged by size:
2789 686 685 393
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 909 1009 1191 1978 2338 909
Size: 100 182 787 360 3124
Fragments arranged by size:
3124 787 360 182 100
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 143 689 1567 143
Size: 546 878 3129
Fragments arranged by size:
3129 878 546
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SanDI GG'GwC_CC
Cuts at: 837 837
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SexAI A'CCwGG_T
Cuts at: 488 488
Size: 4553
SfcI C'TryA_G
Cuts at: 33 517 910 1218 1596 2281 3274 3465 33
Size: 484 393 308 378 685 993 191 1121
Fragments arranged by size:
1121 993 685 484 393 378 308 191
SfoI GGC'GCC
Cuts at: 391 442 1985 2233 391
Size: 51 1543 248 2711
Fragments arranged by size:
2711 1543 248 51
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 167 1575 167
Size: 1408 3145
SmlI C'TyrA_G
Cuts at: 142 230 289 1369 1708 2623 3115 3377 142
Size: 88 59 1080 339 915 492 262 1318
Fragments arranged by size:
1318 1080 915 492 339 262 88 59
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 903 1500 1606 2106 903
Size: 597 106 500 3350
Fragments arranged by size:
3350 597 500 106
StuI AGG'CCT
Cuts at: 646 646
Size: 4553
StyI C'CwwG_G
Cuts at: 425 616 629 1146 1687 2041 425
Size: 191 13 517 541 354 2937
Fragments arranged by size:
2937 541 517 354 191 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 727 1049 2136 2269 2728 2937 727
Size: 322 1087 133 459 209 2343
Fragments arranged by size:
2343 1087 459 322 209 133
TatI w'GTAC_w
Cuts at: 1424 4068 1424
Size: 2644 1909
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 112 144 405 437 658 690 712 744 4070
Size: 32 261 32 221 32 22 32 3326
Cuts at: 4070 4102 112
Size: 32 563
Fragments arranged by size:
3326 563 261 221 32 32 32 32
32 22
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1306 2048 1306
Size: 742 3811
XhoI C'TCGA_G
Cuts at: 230 289 230
Size: 59 4494
XmaI C'CCGG_G
Cuts at: 165 1573 165
Size: 1408 3145
ZraI GAC'GTC
Cuts at: 941 941
Size: 4553
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI