Restriction map for
pF1KB4666
(Circular) (Six-base) MAPSORT of: pF1KB4666.seq Check: 237 from: 1 to: 3317
pF1KB4666 3317 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 355 355
Size: 3317
Acc65I G'GTAC_C
Cuts at: 333 333
Size: 3317
AclI AA'CG_TT
Cuts at: 3157 3157
Size: 3317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1142 1574 2321 2589 1142
Size: 432 747 268 1870
Fragments arranged by size:
1870 747 432 268
AfeI AGC'GCT
Cuts at: 142 142
Size: 3317
AflIII A'CryG_T
Cuts at: 669 1773 2750 669
Size: 1104 977 1236
Fragments arranged by size:
1236 1104 977
AgeI A'CCGG_T
Cuts at: 1705 1705
Size: 3317
AlwNI CAG_nnn'CTG
Cuts at: 622 2189 622
Size: 1567 1750
ApaLI G'TGCA_C
Cuts at: 2087 2087
Size: 3317
ApoI r'AATT_y
Cuts at: 321 2670 2926 321
Size: 2349 256 712
Fragments arranged by size:
2349 712 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3317
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3317
AvaI C'yCGr_G
Cuts at: 337 337
Size: 3317
BamHI G'GATC_C
Cuts at: 342 342
Size: 3317
BanI G'GyrC_C
Cuts at: 333 747 995 1030 333
Size: 414 248 35 2620
Fragments arranged by size:
2620 414 248 35
BanII G_rGCy'C
Cuts at: 331 1361 331
Size: 1030 2287
BbeI G_GCGC'C
Cuts at: 751 999 751
Size: 248 3069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 117 151 117
Size: 34 3283
BciVI GTATCCnnnnn_n'
Cuts at: 1215 1976 3317 1215
Size: 761 1341 1215
Fragments arranged by size:
1341 1215 761
BclI T'GATC_A
Cuts at: 837 837
Size: 3317
BglII A'GATC_T
Cuts at: 832 2869 832
Size: 2037 1280
BlpI GC'TnA_GC
Cuts at: 429 429
Size: 3317
Bme1580I G_kGCm'C
Cuts at: 942 1035 2091 942
Size: 93 1056 2168
Fragments arranged by size:
2168 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 529 610 945 3089 529
Size: 81 335 2144 757
Fragments arranged by size:
2144 757 335 81
BmtI G_CTAG'C
Cuts at: 125 125
Size: 3317
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 493 1372 1864 2162 142
Size: 351 879 492 298 1297
Fragments arranged by size:
1297 879 492 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 195 3021 195
Size: 2826 491
BsaAI yAC'GTr
Cuts at: 670 670
Size: 3317
BsaBI GATnn'nnATC
Cuts at: 6 831 2868 6
Size: 825 2037 455
Fragments arranged by size:
2037 825 455
BsaHI Gr'CG_yC
Cuts at: 748 996 1698 3194 748
Size: 248 702 1496 871
Fragments arranged by size:
1496 871 702 248
BsaWI w'CCGG_w
Cuts at: 506 1027 1705 1979 2126 2537 506
Size: 521 678 274 147 411 1286
Fragments arranged by size:
1286 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 742 1500 2077 2573 742
Size: 758 577 496 1486
Fragments arranged by size:
1486 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 859 905 2113 104
Size: 755 46 1208 1308
Fragments arranged by size:
1308 1208 755 46
BsiHKAI G_wGCw'C
Cuts at: 331 1109 1299 2091 331
Size: 778 190 792 1557
Fragments arranged by size:
1557 792 778 190
BsmI GAATG_Cn'
Cuts at: 237 237
Size: 3317
Bsp1286I G_dGCh'C
Cuts at: 331 942 1035 1109 1299 1361 2091 331
Size: 611 93 74 190 62 730 1557
Fragments arranged by size:
1557 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2537 2537
Size: 3317
BspHI T'CATG_A
Cuts at: 2493 2493
Size: 3317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 367 581 883 1264 367
Size: 214 302 381 2420
Fragments arranged by size:
2420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1609 1663 3147 3312 50
Size: 1559 54 1484 165 55
Fragments arranged by size:
1559 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1229 1229
Size: 3317
BsrFI r'CCGG_y
Cuts at: 1315 1496 1705 1315
Size: 181 209 2927
Fragments arranged by size:
2927 209 181
BssSI C'ACGA_G
Cuts at: 1588 1946 1588
Size: 358 2959
BstAPI GCAn_nnn'nTGC
Cuts at: 694 694
Size: 3317
BstBI TT'CG_AA
Cuts at: 1678 1678
Size: 3317
BstYI r'GATC_y
Cuts at: 1 295 342 809 832 1167 1413 2425 2511
Size: 294 47 467 23 335 246 1012 86
Cuts at: 2511 2523 2869 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 294 246 86
47 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1267 1448 1611 1267
Size: 181 163 2973
Fragments arranged by size:
2973 181 163
BtsI GCAGTG_nn'
Cuts at: 560 560
Size: 3317
DraI TTT'AAA
Cuts at: 316 316
Size: 3317
DrdI GACnn_nn'nnGTC
Cuts at: 1023 1881 1023
Size: 858 2459
EaeI y'GGCC_r
Cuts at: 902 1076 1467 1494 2839 902
Size: 174 391 27 1345 1380
Fragments arranged by size:
1380 1345 391 174 27
EagI C'GGCC_G
Cuts at: 902 902
Size: 3317
EarI CTCTTCn'nnn_
Cuts at: 1340 1550 1340
Size: 210 3107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1525 1835 1981 3127 1525
Size: 310 146 1146 1715
Fragments arranged by size:
1715 1146 310 146
EcoICRI GAG'CTC
Cuts at: 329 329
Size: 3317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1142 1574 2321 2589 1142
Size: 432 747 268 1870
Fragments arranged by size:
1870 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 456 456
Size: 3317
EcoRI G'AATT_C
Cuts at: 321 321
Size: 3317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1336 1336
Size: 3317
FspI TGC'GCA
Cuts at: 1098 1098
Size: 3317
HaeII r_GCGC'y
Cuts at: 134 144 751 999 2021 3000 134
Size: 10 607 248 1022 979 451
Fragments arranged by size:
1022 979 607 451 248 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 1159 1189 1191 1221 280
Size: 32 847 30 2 30 2376
Fragments arranged by size:
2376 847 32 30 30 2
HincII GTy'rAC
Cuts at: 356 356
Size: 3317
KasI G'GCGC_C
Cuts at: 747 995 747
Size: 248 3069
KpnI G_GTAC'C
Cuts at: 337 337
Size: 3317
MluI A'CGCG_T
Cuts at: 2750 2750
Size: 3317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 1988 2172 143
Size: 1845 184 1288
Fragments arranged by size:
1845 1288 184
MscI TGG'CCA
Cuts at: 1078 1078
Size: 3317
MslI CAynn'nnrTG
Cuts at: 1306 1433 1306
Size: 127 3190
MspA1I CmG'CkG
Cuts at: 202 268 428 742 1102 2115 2360 202
Size: 66 160 314 360 1013 245 1159
Fragments arranged by size:
1159 1013 360 314 245 160 66
NaeI GCC'GGC
Cuts at: 1498 1498
Size: 3317
NarI GG'CG_CC
Cuts at: 748 996 748
Size: 248 3069
NgoMIV G'CCGG_C
Cuts at: 1496 1496
Size: 3317
NheI G'CTAG_C
Cuts at: 121 121
Size: 3317
NspI r_CATG'y
Cuts at: 370 870 1777 370
Size: 500 907 1910
Fragments arranged by size:
1910 907 500
PciI A'CATG_T
Cuts at: 1773 1773
Size: 3317
PfoI T'CCnGG_A
Cuts at: 284 284
Size: 3317
PmeI GTTT'AAAC
Cuts at: 316 316
Size: 3317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2456 2488 2456
Size: 32 3285
PstI C_TGCA'G
Cuts at: 140 207 364 1049 140
Size: 67 157 685 2408
Fragments arranged by size:
2408 685 157 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3317
PvuII CAG'CTG
Cuts at: 202 268 742 1102 202
Size: 66 474 360 2417
Fragments arranged by size:
2417 474 360 66
RsrII CG'GwC_CG
Cuts at: 1512 1512
Size: 3317
SacI G_AGCT'C
Cuts at: 331 331
Size: 3317
SalI G'TCGA_C
Cuts at: 354 354
Size: 3317
SapI GCTCTTCn'nnn_
Cuts at: 1340 1550 1340
Size: 210 3107
SbfI CC_TGCA'GG
Cuts at: 364 364
Size: 3317
ScaI AGT'ACT
Cuts at: 220 2834 220
Size: 2614 703
SfcI C'TryA_G
Cuts at: 33 136 203 360 1045 2038 2229 33
Size: 103 67 157 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 157 103 67
SfoI GGC'GCC
Cuts at: 749 997 749
Size: 248 3069
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3317
SmaI CCC'GGG
Cuts at: 339 339
Size: 3317
SmlI C'TyrA_G
Cuts at: 157 472 1387 1879 2141 157
Size: 315 915 492 262 1333
Fragments arranged by size:
1333 915 492 315 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3317
SphI G_CATG'C
Cuts at: 370 870 370
Size: 500 2817
StyI C'CwwG_G
Cuts at: 451 805 451
Size: 354 2963
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 247 900 1033 1492 1701 247
Size: 653 133 459 209 1863
Fragments arranged by size:
1863 653 459 209 133
TatI w'GTAC_w
Cuts at: 218 2832 218
Size: 2614 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3285
Tth111I GACn'n_nGTC
Cuts at: 1114 1114
Size: 3317
XbaI T'CTAG_A
Cuts at: 348 348
Size: 3317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 812 812
Size: 3317
XmaI C'CCGG_G
Cuts at: 337 337
Size: 3317
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI