Restriction map for
pF1KB4674
(Circular) (Six-base) MAPSORT of: pF1KB4674.seq Check: 1514 from: 1 to: 4289
pF1KB4674 4289 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 246 246
Size: 4289
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 2114 2546 3293 3561 728
Size: 1386 432 747 268 1456
Fragments arranged by size:
1456 1386 747 432 268
AflIII A'CryG_T
Cuts at: 1641 2745 3722 1641
Size: 1104 977 2208
Fragments arranged by size:
2208 1104 977
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 163 1594 3161 163
Size: 1431 1567 1291
Fragments arranged by size:
1567 1431 1291
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 545 1293 3642 3898 545
Size: 748 2349 256 936
Fragments arranged by size:
2349 936 748 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 293 1309 293
Size: 1016 3273
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 1305 1719 1967 2002 1305
Size: 414 248 35 3592
Fragments arranged by size:
3592 414 248 35
BanII G_rGCy'C
Cuts at: 293 687 1303 2333 293
Size: 394 616 1030 2249
Fragments arranged by size:
2249 1030 616 394
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbvCI CC'TCA_GC
Cuts at: 164 164
Size: 4289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 194 160
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 579 1064 1274 1809 579
Size: 485 210 535 3059
Fragments arranged by size:
3059 535 485 210
BfrBI ATG'CAT
Cuts at: 791 791
Size: 4289
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 173 943 1914 2007 3063 173
Size: 770 971 93 1056 1399
Fragments arranged by size:
1399 1056 971 770 93
BmrI ACTGGGnnnn_n'
Cuts at: 1501 1582 1917 4061 1501
Size: 81 335 2144 1729
Fragments arranged by size:
2144 1729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 4289
Bpu10I CC'TnA_GC
Cuts at: 148 164 148
Size: 16 4273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2344 2836 3134 1465
Size: 879 492 298 2620
Fragments arranged by size:
2620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3993 3993
Size: 4289
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 803 1803 3840 6
Size: 797 1000 2037 455
Fragments arranged by size:
2037 1000 797 455
BsaHI Gr'CG_yC
Cuts at: 177 1720 1968 2670 4166 177
Size: 1543 248 702 1496 300
Fragments arranged by size:
1543 1496 702 300 248
BsaWI w'CCGG_w
Cuts at: 285 653 1478 1999 2677 2951 3098 3509 285
Size: 368 825 521 678 274 147 411 1065
Fragments arranged by size:
1065 825 678 521 411 368 274 147
BseYI C'CCAG_C
Cuts at: 1714 2472 3049 3545 1714
Size: 758 577 496 2458
Fragments arranged by size:
2458 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1303 2081 2271 3063 1303
Size: 778 190 792 2529
Fragments arranged by size:
2529 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 173 293 687 943 1303 1914 2007 2081 2271
Size: 120 394 256 360 611 93 74 190
Cuts at: 2271 2333 3063 173
Size: 62 730 1399
Fragments arranged by size:
1399 730 611 394 360 256 190 120
93 74 62
BspEI T'CCGG_A
Cuts at: 285 3509 285
Size: 3224 1065
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 246 1339 1553 1855 2236 246
Size: 1093 214 302 381 2299
Fragments arranged by size:
2299 1093 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 536 723 2201 536
Size: 187 1478 2624
Fragments arranged by size:
2624 1478 187
BsrFI r'CCGG_y
Cuts at: 130 2287 2468 2677 130
Size: 2157 181 209 1742
Fragments arranged by size:
2157 1742 209 181
BssSI C'ACGA_G
Cuts at: 1093 2560 2918 1093
Size: 1467 358 2464
Fragments arranged by size:
2464 1467 358
BstAPI GCAn_nnn'nTGC
Cuts at: 718 1666 718
Size: 948 3341
BstBI TT'CG_AA
Cuts at: 1266 2650 1266
Size: 1384 2905
BstXI CCAn_nnnn'nTGG
Cuts at: 1244 1244
Size: 4289
BstYI r'GATC_y
Cuts at: 1 282 369 624 1314 1781 1804 2139 2385
Size: 281 87 255 690 467 23 335 246
Cuts at: 2385 3397 3483 3495 3841 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 690 467 449 346 335 281 255
246 87 86 23 12
BtgI C'CryG_G
Cuts at: 107 974 107
Size: 867 3422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 1532 1532
Size: 4289
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 362 1874 2048 2439 2466 3811 362
Size: 1512 174 391 27 1345 840
Fragments arranged by size:
1512 1345 840 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2497 2807 2953 4099 2497
Size: 310 146 1146 2687
Fragments arranged by size:
2687 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 728 2114 2546 3293 3561 545
Size: 183 1386 432 747 268 1273
Fragments arranged by size:
1386 1273 747 432 268 183
EcoO109I rG'GnC_Cy
Cuts at: 1428 1428
Size: 4289
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 796 1006 2308 796
Size: 210 1302 2777
Fragments arranged by size:
2777 1302 210
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 1723 1971 2993 3972 1723
Size: 248 1022 979 2040
Fragments arranged by size:
2040 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1070 1102 2131 2161 2163 2193 491
Size: 32 547 32 1029 30 2 30 2587
Fragments arranged by size:
2587 1029 547 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
HindIII A'AGCT_T
Cuts at: 988 988
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 477 2960 3144 477
Size: 2483 184 1622
Fragments arranged by size:
2483 1622 184
MscI TGG'CCA
Cuts at: 364 2050 364
Size: 1686 2603
MslI CAynn'nnrTG
Cuts at: 2278 2405 2278
Size: 127 4162
MspA1I CmG'CkG
Cuts at: 850 1400 1714 2074 3087 3332 850
Size: 550 314 360 1013 245 1807
Fragments arranged by size:
1807 1013 550 360 314 245
NaeI GCC'GGC
Cuts at: 132 2470 132
Size: 2338 1951
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 107 974 107
Size: 867 3422
NgoMIV G'CCGG_C
Cuts at: 130 2468 130
Size: 2338 1951
NsiI A_TGCA'T
Cuts at: 793 793
Size: 4289
NspI r_CATG'y
Cuts at: 1342 1842 2749 1342
Size: 500 907 2882
Fragments arranged by size:
2882 907 500
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 815 847 815
Size: 32 4257
PstI C_TGCA'G
Cuts at: 381 1336 2021 381
Size: 955 685 2649
Fragments arranged by size:
2649 955 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 1714 2074 1714
Size: 360 3929
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 377 863 1136 1332 2017 3010 3201 33
Size: 344 486 273 196 685 993 191 1121
Fragments arranged by size:
1121 993 685 486 344 273 196 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 295 1311 295
Size: 1016 3273
SmlI C'TyrA_G
Cuts at: 1444 2359 2851 3113 1444
Size: 915 492 262 2620
Fragments arranged by size:
2620 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
SspI AAT'ATT
Cuts at: 386 386
Size: 4289
StyI C'CwwG_G
Cuts at: 107 347 661 974 1033 1423 1777 107
Size: 240 314 313 59 390 354 2619
Fragments arranged by size:
2619 390 354 314 313 240 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1872 2005 2464 2673 1872
Size: 133 459 209 3488
Fragments arranged by size:
3488 459 209 133
TatI w'GTAC_w
Cuts at: 3804 3804
Size: 4289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 725 757 1191 1223 3806 3838 725
Size: 32 434 32 2583 32 1176
Fragments arranged by size:
2583 1176 434 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1784 1784
Size: 4289
XmaI C'CCGG_G
Cuts at: 293 1309 293
Size: 1016 3273
XmnI GAAnn'nnTTC
Cuts at: 325 325
Size: 4289
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI