Restriction map for
pF1KB4677
(Circular) (Six-base) MAPSORT of: pF1KB4677.seq Check: 7779 from: 1 to: 6026
pF1KB4677 6026 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 189 189
Size: 6026
AccI GT'mk_AC
Cuts at: 2544 2689 2914 3064 2544
Size: 145 225 150 5506
Fragments arranged by size:
5506 225 150 145
Acc65I G'GTAC_C
Cuts at: 2990 3042 2990
Size: 52 5974
AclI AA'CG_TT
Cuts at: 5866 5866
Size: 6026
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 1058 1790 2161 2425 2980 3851 4283 4636
Size: 780 732 371 264 555 871 432 353
Cuts at: 4636 5298 278
Size: 662 1006
Fragments arranged by size:
1006 871 780 732 662 555 432 371
353 264
AfeI AGC'GCT
Cuts at: 2972 2972
Size: 6026
AflIII A'CryG_T
Cuts at: 1958 3378 5178 5459 1958
Size: 1420 1800 281 2525
Fragments arranged by size:
2525 1800 1420 281
AhdI GACnn_n'nnGTC
Cuts at: 2142 2142
Size: 6026
AlwNI CAG_nnn'CTG
Cuts at: 2316 3331 4769 2316
Size: 1015 1438 3573
Fragments arranged by size:
3573 1438 1015
ApaLI G'TGCA_C
Cuts at: 397 4864 397
Size: 4467 1559
ApoI r'AATT_y
Cuts at: 199 356 425 595 793 961 1002 1165 1534
Size: 157 69 170 198 168 41 163 369
Cuts at: 1534 2022 2080 2285 2445 2594 2882 3030 5379
Size: 488 58 205 160 149 288 148 2349
Cuts at: 5379 5635 199
Size: 256 590
Fragments arranged by size:
2349 590 488 369 288 256 205 198
170 168 163 160 157 149 148 69
58 41
AseI AT'TA_AT
Cuts at: 20 545 1631 2489 20
Size: 525 1086 858 3557
Fragments arranged by size:
3557 1086 858 525
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6026
AvaI C'yCGr_G
Cuts at: 3046 3046
Size: 6026
BamHI G'GATC_C
Cuts at: 3051 3051
Size: 6026
BanI G'GyrC_C
Cuts at: 2990 3042 3456 3704 3739 2990
Size: 52 414 248 35 5277
Fragments arranged by size:
5277 414 248 52 35
BanII G_rGCy'C
Cuts at: 905 3040 4070 905
Size: 2135 1030 2861
Fragments arranged by size:
2861 2135 1030
BbeI G_GCGC'C
Cuts at: 3460 3708 3460
Size: 248 5778
BbsI GAAGACnn'nnnn_
Cuts at: 2138 2138
Size: 6026
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1268 1302 1268
Size: 34 5992
BciVI GTATCCnnnnn_n'
Cuts at: 3924 4980 6026 3924
Size: 1056 1046 3924
Fragments arranged by size:
3924 1056 1046
BclI T'GATC_A
Cuts at: 3546 3546
Size: 6026
BglII A'GATC_T
Cuts at: 2627 3541 5578 2627
Size: 914 2037 3075
Fragments arranged by size:
3075 2037 914
BlpI GC'TnA_GC
Cuts at: 409 3138 409
Size: 2729 3297
Bme1580I G_kGCm'C
Cuts at: 401 2517 3651 3744 4868 401
Size: 2116 1134 93 1124 1559
Fragments arranged by size:
2116 1559 1134 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3238 3319 3654 5798 3238
Size: 81 335 2144 3466
Fragments arranged by size:
3466 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4393 4393
Size: 6026
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 348 2959 277
Size: 71 2611 3344
Fragments arranged by size:
3344 2611 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3202 4081 4795 5093 3202
Size: 879 714 298 4135
Fragments arranged by size:
4135 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1080 5730 1080
Size: 4650 1376
BsaAI yAC'GTr
Cuts at: 3379 3379
Size: 6026
BsaBI GATnn'nnATC
Cuts at: 6 125 170 3540 5577 6
Size: 119 45 3370 2037 455
Fragments arranged by size:
3370 2037 455 119 45
BsaHI Gr'CG_yC
Cuts at: 186 3457 3705 4407 5903 186
Size: 3271 248 702 1496 309
Fragments arranged by size:
3271 1496 702 309 248
BsaWI w'CCGG_w
Cuts at: 3215 3736 4414 4825 4972 5246 3215
Size: 521 678 411 147 274 3995
Fragments arranged by size:
3995 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2023 2053 2023
Size: 30 5996
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1222 2044 1222
Size: 822 5204
BseYI C'CCAG_C
Cuts at: 2833 3451 4209 4874 5282 2833
Size: 618 758 665 408 3577
Fragments arranged by size:
3577 758 665 618 408
BsiEI CG_ry'CG
Cuts at: 104 372 3568 3614 4844 104
Size: 268 3196 46 1230 1286
Fragments arranged by size:
3196 1286 1230 268 46
BsiHKAI G_wGCw'C
Cuts at: 401 905 2378 2953 3040 3818 4008 4868 401
Size: 504 1473 575 87 778 190 860 1559
Fragments arranged by size:
1559 1473 860 778 575 504 190 87
BsmI GAATG_Cn'
Cuts at: 2236 2967 2236
Size: 731 5295
Bsp1286I G_dGCh'C
Cuts at: 401 905 2378 2517 2953 3040 3651 3744 3818
Size: 504 1473 139 436 87 611 93 74
Cuts at: 3818 4008 4070 4868 401
Size: 190 62 798 1559
Fragments arranged by size:
1559 1473 798 611 504 436 190 139
93 87 74 62
BspHI T'CATG_A
Cuts at: 591 1835 4458 591
Size: 1244 2623 2159
Fragments arranged by size:
2623 2159 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 983 3076 3290 3592 3973 983
Size: 2093 214 302 381 3036
Fragments arranged by size:
3036 2093 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4318 4372 5856 6021 50
Size: 4268 54 1484 165 55
Fragments arranged by size:
4268 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3938 3938
Size: 6026
BsrFI r'CCGG_y
Cuts at: 889 4024 4205 889
Size: 3135 181 2710
Fragments arranged by size:
3135 2710 181
BssSI C'ACGA_G
Cuts at: 4297 5005 4297
Size: 708 5318
BstAPI GCAn_nnn'nTGC
Cuts at: 405 709 940 1678 2272 3403 405
Size: 304 231 738 594 1131 3028
Fragments arranged by size:
3028 1131 738 594 304 231
BstBI TT'CG_AA
Cuts at: 4387 4387
Size: 6026
BstXI CCAn_nnnn'nTGG
Cuts at: 485 2189 485
Size: 1704 4322
BstYI r'GATC_y
Cuts at: 1 783 1234 2627 3051 3518 3541 3876 4122
Size: 782 451 1393 424 467 23 335 246
Cuts at: 4122 4428 4440 4526 5578 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1393 1052 782 467 451 449 424 335
306 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2545 2690 2545
Size: 145 5881
Bsu36I CC'TnA_GG
Cuts at: 721 721
Size: 6026
BtgI C'CryG_G
Cuts at: 107 107
Size: 6026
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 134 3976 4157 4320 134
Size: 3842 181 163 1840
Fragments arranged by size:
3842 1840 181 163
BtsI GCAGTG_nn'
Cuts at: 3013 3269 3013
Size: 256 5770
DraI TTT'AAA
Cuts at: 152 579 1514 3025 152
Size: 427 935 1511 3153
Fragments arranged by size:
3153 1511 935 427
DrdI GACnn_nn'nnGTC
Cuts at: 3732 5076 3732
Size: 1344 4682
EaeI y'GGCC_r
Cuts at: 377 3611 3785 4176 4203 5548 377
Size: 3234 174 391 27 1345 855
Fragments arranged by size:
3234 1345 855 391 174 27
EagI C'GGCC_G
Cuts at: 3611 3611
Size: 6026
EarI CTCTTCn'nnn_
Cuts at: 875 1809 4049 4259 875
Size: 934 2240 210 2642
Fragments arranged by size:
2642 2240 934 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4234 4976 5122 5836 4234
Size: 742 146 714 4424
Fragments arranged by size:
4424 742 714 146
EcoICRI GAG'CTC
Cuts at: 903 3038 903
Size: 2135 3891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 278 348 1058 1790 2161 2425 2959 2980
Size: 1 70 710 732 371 264 534 21
Cuts at: 2980 3851 4283 4636 5298 277
Size: 871 432 353 662 1005
Fragments arranged by size:
1005 871 732 710 662 534 432 371
353 264 70 21 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1310 1310
Size: 6026
EcoO109I rG'GnC_Cy
Cuts at: 1303 1708 3165 1303
Size: 405 1457 4164
Fragments arranged by size:
4164 1457 405
EcoRI G'AATT_C
Cuts at: 961 2445 3030 961
Size: 1484 585 3957
Fragments arranged by size:
3957 1484 585
EcoRV GAT'ATC
Cuts at: 2803 2803
Size: 6026
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1621 2009 3010 4045 1621
Size: 388 1001 1035 3602
Fragments arranged by size:
3602 1035 1001 388
FspI TGC'GCA
Cuts at: 3807 3807
Size: 6026
HaeII r_GCGC'y
Cuts at: 2974 3460 3708 4938 5709 2974
Size: 486 248 1230 771 3291
Fragments arranged by size:
3291 1230 771 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1501 1533 1620 1652 2128 2160 3868 3898 3900
Size: 32 87 32 476 32 1708 30 2
Cuts at: 3900 3930 1501
Size: 30 3597
Fragments arranged by size:
3597 1708 476 87 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1374 2397 3065 1374
Size: 1023 668 4335
Fragments arranged by size:
4335 1023 668
HindIII A'AGCT_T
Cuts at: 628 859 628
Size: 231 5795
KasI G'GCGC_C
Cuts at: 3456 3704 3456
Size: 248 5778
KpnI G_GTAC'C
Cuts at: 2994 3046 2994
Size: 52 5974
MluI A'CGCG_T
Cuts at: 5459 5459
Size: 6026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 4785 4969 207
Size: 4578 184 1264
Fragments arranged by size:
4578 1264 184
MscI TGG'CCA
Cuts at: 379 3787 379
Size: 3408 2618
MslI CAynn'nnrTG
Cuts at: 483 704 1388 2132 4015 4142 483
Size: 221 684 744 1883 127 2367
Fragments arranged by size:
2367 1883 744 684 221 127
MspA1I CmG'CkG
Cuts at: 193 1735 2278 3137 3451 3811 4595 4840 193
Size: 1542 543 859 314 360 784 245 1379
Fragments arranged by size:
1542 1379 859 784 543 360 314 245
NaeI GCC'GGC
Cuts at: 891 4207 891
Size: 3316 2710
NarI GG'CG_CC
Cuts at: 3457 3705 3457
Size: 248 5778
NcoI C'CATG_G
Cuts at: 107 107
Size: 6026
NgoMIV G'CCGG_C
Cuts at: 889 4205 889
Size: 3316 2710
NspI r_CATG'y
Cuts at: 1962 2382 3079 3579 5182 1962
Size: 420 697 500 1603 2806
Fragments arranged by size:
2806 1603 697 500 420
PciI A'CATG_T
Cuts at: 1958 5178 1958
Size: 3220 2806
PflMI CCAn_nnn'nTGG
Cuts at: 2699 2699
Size: 6026
PfoI T'CCnGG_A
Cuts at: 2925 2925
Size: 6026
PmeI GTTT'AAAC
Cuts at: 3025 3025
Size: 6026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 5994
PpuMI rG'GwC_Cy
Cuts at: 1303 1303
Size: 6026
PsiI TTA'TAA
Cuts at: 1097 1097
Size: 6026
PstI C_TGCA'G
Cuts at: 694 1349 1740 2277 3073 3758 694
Size: 655 391 537 796 685 2962
Fragments arranged by size:
2962 796 685 655 537 391
PvuI CG_AT'CG
Cuts at: 104 372 104
Size: 268 5758
PvuII CAG'CTG
Cuts at: 193 1735 2278 3451 3811 193
Size: 1542 543 1173 360 2408
Fragments arranged by size:
2408 1542 1173 543 360
RsrII CG'GwC_CG
Cuts at: 4221 4221
Size: 6026
SacI G_AGCT'C
Cuts at: 905 3040 905
Size: 2135 3891
SalI G'TCGA_C
Cuts at: 3063 3063
Size: 6026
SapI GCTCTTCn'nnn_
Cuts at: 1809 4049 4259 1809
Size: 2240 210 3576
Fragments arranged by size:
3576 2240 210
SbfI CC_TGCA'GG
Cuts at: 3073 3073
Size: 6026
ScaI AGT'ACT
Cuts at: 5543 5543
Size: 6026
SfcI C'TryA_G
Cuts at: 33 690 765 1345 1736 2273 2855 3069 3754
Size: 657 75 580 391 537 582 214 685
Cuts at: 3754 4722 4913 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 657 582 580 537 391
214 191 75
SfoI GGC'GCC
Cuts at: 3458 3706 3458
Size: 248 5778
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6026
SmaI CCC'GGG
Cuts at: 3048 3048
Size: 6026
SmlI C'TyrA_G
Cuts at: 3181 4096 4810 5072 3181
Size: 915 714 262 4135
Fragments arranged by size:
4135 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1008 67
Size: 941 5085
SphI G_CATG'C
Cuts at: 3079 3579 3079
Size: 500 5526
SspI AAT'ATT
Cuts at: 148 2208 2534 148
Size: 2060 326 3640
Fragments arranged by size:
3640 2060 326
StyI C'CwwG_G
Cuts at: 107 2343 2985 3160 3514 107
Size: 2236 642 175 354 2619
Fragments arranged by size:
2619 2236 642 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3609 3742 4201 4410 3609
Size: 133 459 209 5225
Fragments arranged by size:
5225 459 209 133
TatI w'GTAC_w
Cuts at: 1211 5541 1211
Size: 4330 1696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5543 5575 5543
Size: 32 5994
Tth111I GACn'n_nGTC
Cuts at: 3823 3823
Size: 6026
XbaI T'CTAG_A
Cuts at: 3057 3057
Size: 6026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 544 1373 2886 3521 544
Size: 829 1513 635 3049
Fragments arranged by size:
3049 1513 829 635
XmaI C'CCGG_G
Cuts at: 3046 3046
Size: 6026
XmnI GAAnn'nnTTC
Cuts at: 473 1845 2084 473
Size: 1372 239 4415
Fragments arranged by size:
4415 1372 239
ZraI GAC'GTC
Cuts at: 187 187
Size: 6026
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII ClaI
CspCI DraIII FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI