Restriction map for
pF1KB4679
(Circular) (Six-base) MAPSORT of: pF1KB4679.seq Check: 3474 from: 1 to: 3716
pF1KB4679 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 529 529
Size: 3716
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 350 732 350
Size: 382 3334
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2326 2988 1541
Size: 432 353 662 2269
Fragments arranged by size:
2269 662 432 353
AflIII A'CryG_T
Cuts at: 209 670 1068 2868 3149 209
Size: 461 398 1800 281 776
Fragments arranged by size:
1800 776 461 398 281
AleI CACnn'nnGTG
Cuts at: 530 530
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 1021 2459 1021
Size: 1438 2278
ApaLI G'TGCA_C
Cuts at: 2554 2554
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 431 736 431
Size: 305 3411
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 691 724 691
Size: 33 3683
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 350 732 1146 1394 1429 350
Size: 382 414 248 35 2637
Fragments arranged by size:
2637 414 382 248 35
BanII G_rGCy'C
Cuts at: 429 730 1760 429
Size: 301 1030 2385
Fragments arranged by size:
2385 1030 301
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbsI GAAGACnn'nnnn_
Cuts at: 317 317
Size: 3716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 564 598 564
Size: 34 3682
BciVI GTATCCnnnnn_n'
Cuts at: 692 1614 2670 3716 692
Size: 922 1056 1046 692
Fragments arranged by size:
1056 1046 922 692
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglI GCCn_nnn'nGGC
Cuts at: 401 401
Size: 3716
BglII A'GATC_T
Cuts at: 649 1231 3268 649
Size: 582 2037 1097
Fragments arranged by size:
2037 1097 582
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 1341 1434 2558 1341
Size: 93 1124 2499
Fragments arranged by size:
2499 1124 93
BmgBI CAC'GTC
Cuts at: 210 210
Size: 3716
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 164
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 892 1771 2485 2783 232
Size: 660 879 714 298 1165
Fragments arranged by size:
1165 879 714 660 298
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 165 673 1069 165
Size: 508 396 2812
Fragments arranged by size:
2812 508 396
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2515 2662 2936 905
Size: 521 678 411 147 274 1685
Fragments arranged by size:
1685 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 107
Size: 30 3686
BseYI C'CCAG_C
Cuts at: 1141 1899 2564 2972 1141
Size: 758 665 408 1885
Fragments arranged by size:
1885 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 384 1258 1304 2534 104
Size: 280 874 46 1230 1286
Fragments arranged by size:
1286 1230 874 280 46
BsiHKAI G_wGCw'C
Cuts at: 429 730 1508 1698 2558 429
Size: 301 778 190 860 1587
Fragments arranged by size:
1587 860 778 301 190
BsmBI CGTCTCn'nnnn_
Cuts at: 296 296
Size: 3716
Bsp1286I G_dGCh'C
Cuts at: 429 730 1341 1434 1508 1698 1760 2558 429
Size: 301 611 93 74 190 62 798 1587
Fragments arranged by size:
1587 798 611 301 190 93 74 62
BspHI T'CATG_A
Cuts at: 654 2148 654
Size: 1494 2222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 529 766 980 1282 1663 529
Size: 237 214 302 381 2582
Fragments arranged by size:
2582 381 302 237 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 1714
Size: 181 3535
BssSI C'ACGA_G
Cuts at: 1987 2695 1987
Size: 708 3008
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 624 2077 624
Size: 1453 2263
BstYI r'GATC_y
Cuts at: 1 649 741 1208 1231 1566 1812 2118 2130
Size: 648 92 467 23 335 246 306 12
Cuts at: 2130 2216 3268 1
Size: 86 1052 449
Fragments arranged by size:
1052 648 467 449 335 306 246 92
86 23 12
BtgI C'CryG_G
Cuts at: 258 384 258
Size: 126 3590
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 518 715 518
Size: 197 3519
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2766 1422
Size: 1344 2372
EaeI y'GGCC_r
Cuts at: 381 1301 1475 1866 1893 3238 381
Size: 920 174 391 27 1345 859
Fragments arranged by size:
1345 920 859 391 174 27
EagI C'GGCC_G
Cuts at: 381 1301 381
Size: 920 2796
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 437 1924 2666 2812 3526 437
Size: 1487 742 146 714 627
Fragments arranged by size:
1487 742 714 627 146
EcoICRI GAG'CTC
Cuts at: 427 728 427
Size: 301 3415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 1541 1973 2326 2988 164
Size: 1377 432 353 662 892
Fragments arranged by size:
1377 892 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 294 855 294
Size: 561 3155
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 230 1150 1398 2628 3399 230
Size: 920 248 1230 771 547
Fragments arranged by size:
1230 920 771 547 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 464 496 1558 1588 1590 1620 464
Size: 32 1062 30 2 30 2560
Fragments arranged by size:
2560 1062 32 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 354 736 354
Size: 382 3334
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2475 2659 2475
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 163 530 1705 1832 163
Size: 367 1175 127 2047
Fragments arranged by size:
2047 1175 367 127
MspA1I CmG'CkG
Cuts at: 260 827 1141 1501 2285 2530 260
Size: 567 314 360 784 245 1446
Fragments arranged by size:
1446 784 567 360 314 245
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NruI TCG'CGA
Cuts at: 329 329
Size: 3716
NspI r_CATG'y
Cuts at: 531 769 1269 2872 531
Size: 238 500 1603 1375
Fragments arranged by size:
1603 1375 500 238
PciI A'CATG_T
Cuts at: 2868 2868
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 196 228 2162 2194 196
Size: 32 1934 32 1718
Fragments arranged by size:
1934 1718 32 32
PpuMI rG'GwC_Cy
Cuts at: 294 294
Size: 3716
PstI C_TGCA'G
Cuts at: 763 1448 763
Size: 685 3031
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 429 730 429
Size: 301 3415
SacII CC_GC'GG
Cuts at: 261 261
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 759 1444 2412 2603 33
Size: 726 685 968 191 1146
Fragments arranged by size:
1146 968 726 685 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 247 871 1786 2500 2762 247
Size: 624 915 714 262 1201
Fragments arranged by size:
1201 915 714 624 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 194 850 1204 194
Size: 656 354 2706
Fragments arranged by size:
2706 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 107 139 3233 3265 107
Size: 32 3094 32 558
Fragments arranged by size:
3094 558 32 32
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 503 1211 503
Size: 708 3008
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BbvCI BfrBI BmtI Bpu10I BseRI BsgI
BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI