Restriction map for
pF1KB4680
(Circular) (Six-base) MAPSORT of: pF1KB4680.seq Check: 2316 from: 1 to: 4679
pF1KB4680 4679 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 909 909
Size: 4679
AccI GT'mk_AC
Cuts at: 129 1717 129
Size: 1588 3091
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2936 3289 3951 2504
Size: 432 353 662 3232
Fragments arranged by size:
3232 662 432 353
AflIII A'CryG_T
Cuts at: 422 653 2031 3831 4112 422
Size: 231 1378 1800 281 989
Fragments arranged by size:
1800 1378 989 281 231
AleI CACnn'nnGTG
Cuts at: 793 793
Size: 4679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 931 931
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 147 730 752 1637 1984 3422 147
Size: 583 22 885 347 1438 1404
Fragments arranged by size:
1438 1404 885 583 347 22
ApaLI G'TGCA_C
Cuts at: 1483 3517 1483
Size: 2034 2645
ApoI r'AATT_y
Cuts at: 346 1683 4032 4288 346
Size: 1337 2349 256 737
Fragments arranged by size:
2349 1337 737 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 1404 1699 1404
Size: 295 4384
BamHI G'GATC_C
Cuts at: 1704 1704
Size: 4679
BanI G'GyrC_C
Cuts at: 1695 2109 2357 2392 1695
Size: 414 248 35 3982
Fragments arranged by size:
3982 414 248 35
BanII G_rGCy'C
Cuts at: 1201 1693 2723 1201
Size: 492 1030 3157
Fragments arranged by size:
3157 1030 492
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BbsI GAAGACnn'nnnn_
Cuts at: 398 1233 1363 398
Size: 835 130 3714
Fragments arranged by size:
3714 835 130
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3633 4679 2577
Size: 1056 1046 2577
Fragments arranged by size:
2577 1056 1046
BclI T'GATC_A
Cuts at: 2199 2199
Size: 4679
BglI GCCn_nnn'nGGC
Cuts at: 1048 1205 1048
Size: 157 4522
BglII A'GATC_T
Cuts at: 1211 2194 4231 1211
Size: 983 2037 1659
Fragments arranged by size:
2037 1659 983
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 1225 1337 1487 2304 2397 3521 1225
Size: 112 150 817 93 1124 2383
Fragments arranged by size:
2383 1124 817 150 112 93
BmgBI CAC'GTC
Cuts at: 396 897 396
Size: 501 4178
BmrI ACTGGGnnnn_n'
Cuts at: 201 569 1425 1891 1972 2307 4451 201
Size: 368 856 466 81 335 2144 429
Fragments arranged by size:
2144 856 466 429 368 335 81
BmtI G_CTAG'C
Cuts at: 403 403
Size: 4679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 555 3046 555
Size: 2491 2188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 4679
Bpu10I CC'TnA_GC
Cuts at: 1195 1273 1465 1195
Size: 78 192 4409
Fragments arranged by size:
4409 192 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1366 1643 1855 2734 3448 3746 1095
Size: 271 277 212 879 714 298 2028
Fragments arranged by size:
2028 879 714 298 277 271 212
BsaI GGTCTCn'nnnn_
Cuts at: 4383 4383
Size: 4679
BsaAI yAC'GTr
Cuts at: 1340 2032 1340
Size: 692 3987
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 2110 2358 3060 4556 2110
Size: 248 702 1496 2233
Fragments arranged by size:
2233 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3478 3625 3899 1868
Size: 521 678 411 147 274 2648
Fragments arranged by size:
2648 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 619 1246 2104 2862 3527 3935 619
Size: 627 858 758 665 408 1363
Fragments arranged by size:
1363 858 758 665 627 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 937 168
Size: 769 3910
BsiEI CG_ry'CG
Cuts at: 104 641 2221 2267 3497 104
Size: 537 1580 46 1230 1286
Fragments arranged by size:
1580 1286 1230 537 46
BsiHKAI G_wGCw'C
Cuts at: 1015 1279 1345 1487 1693 2471 2661 3521 1015
Size: 264 66 142 206 778 190 860 2173
Fragments arranged by size:
2173 860 778 264 206 190 142 66
BsmBI CGTCTCn'nnnn_
Cuts at: 1188 1188
Size: 4679
Bsp1286I G_dGCh'C
Cuts at: 1015 1201 1225 1279 1337 1345 1487 1693 2304
Size: 186 24 54 58 8 142 206 611
Cuts at: 2304 2397 2471 2661 2723 3521 1015
Size: 93 74 190 62 798 2173
Fragments arranged by size:
2173 798 611 206 190 186 142 93
74 62 58 54 24 8
BspHI T'CATG_A
Cuts at: 3111 3111
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 909 1517 1729 1943 2245 2626 909
Size: 608 212 214 302 381 2962
Fragments arranged by size:
2962 608 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2591 2591
Size: 4679
BsrFI r'CCGG_y
Cuts at: 1038 2677 2858 1038
Size: 1639 181 2859
Fragments arranged by size:
2859 1639 181
BsrGI T'GTAC_A
Cuts at: 1373 1550 1373
Size: 177 4502
BssSI C'ACGA_G
Cuts at: 1390 2950 3658 1390
Size: 1560 708 2411
Fragments arranged by size:
2411 1560 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1433 1517 2056 1433
Size: 84 539 4056
Fragments arranged by size:
4056 539 84
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstYI r'GATC_y
Cuts at: 1 1211 1265 1704 2171 2194 2529 2775 3081
Size: 1210 54 439 467 23 335 246 306
Cuts at: 3081 3093 3179 4231 1
Size: 12 86 1052 449
Fragments arranged by size:
1210 1052 467 449 439 335 306 246
86 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 1057 1057
Size: 4679
BtgI C'CryG_G
Cuts at: 107 473 1202 1478 107
Size: 366 729 276 3308
Fragments arranged by size:
3308 729 366 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1922 1922
Size: 4679
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DraIII CAC_nnn'GTG
Cuts at: 1433 1433
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 835 2385 3729 835
Size: 1550 1344 1785
Fragments arranged by size:
1785 1550 1344
EaeI y'GGCC_r
Cuts at: 638 2264 2438 2829 2856 4201 638
Size: 1626 174 391 27 1345 1116
Fragments arranged by size:
1626 1345 1116 391 174 27
EagI C'GGCC_G
Cuts at: 638 2264 638
Size: 1626 3053
EarI CTCTTCn'nnn_
Cuts at: 891 2702 2912 891
Size: 1811 210 2658
Fragments arranged by size:
2658 1811 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 716 2887 3629 3775 4489 716
Size: 2171 742 146 714 906
Fragments arranged by size:
2171 906 742 714 146
EcoICRI GAG'CTC
Cuts at: 1691 1691
Size: 4679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 2504 2936 3289 3951 352
Size: 2152 432 353 662 1080
Fragments arranged by size:
2152 1080 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 795 916 1060 795
Size: 121 144 4414
Fragments arranged by size:
4414 144 121
EcoO109I rG'GnC_Cy
Cuts at: 1545 1818 1545
Size: 273 4406
EcoRI G'AATT_C
Cuts at: 346 1683 346
Size: 1337 3342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4679
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 1389 2113 2361 3591 4362 1389
Size: 724 248 1230 771 1706
Fragments arranged by size:
1706 1230 771 724 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 757 789 2521 2551 2553 2583 757
Size: 32 1732 30 2 30 2853
Fragments arranged by size:
2853 1732 32 30 30 2
HincII GTy'rAC
Cuts at: 816 1329 1718 816
Size: 513 389 3777
Fragments arranged by size:
3777 513 389
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1211 3438 3622 1211
Size: 2227 184 2268
Fragments arranged by size:
2268 2227 184
MscI TGG'CCA
Cuts at: 2440 2440
Size: 4679
MslI CAynn'nnrTG
Cuts at: 425 658 793 2668 2795 425
Size: 233 135 1875 127 2309
Fragments arranged by size:
2309 1875 233 135 127
MspA1I CmG'CkG
Cuts at: 276 406 730 1204 1790 2104 2464 3248 3493
Size: 130 324 474 586 314 360 784 245
Cuts at: 3493 276
Size: 1462
Fragments arranged by size:
1462 784 586 474 360 324 314 245
130
NaeI GCC'GGC
Cuts at: 1040 2860 1040
Size: 1820 2859
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NcoI C'CATG_G
Cuts at: 107 107
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 1038 2858 1038
Size: 1820 2859
NheI G'CTAG_C
Cuts at: 399 399
Size: 4679
NspI r_CATG'y
Cuts at: 249 426 657 1374 1427 1732 2232 3835 249
Size: 177 231 717 53 305 500 1603 1093
Fragments arranged by size:
1603 1093 717 500 305 231 177 53
PciI A'CATG_T
Cuts at: 422 653 3831 422
Size: 231 3178 1270
Fragments arranged by size:
3178 1270 231
PflMI CCAn_nnn'nTGG
Cuts at: 1479 1479
Size: 4679
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PmlI CAC'GTG
Cuts at: 1340 1340
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 278 310 1513 1545 3125 3157 278
Size: 32 1203 32 1580 32 1800
Fragments arranged by size:
1800 1580 1203 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1545 1545
Size: 4679
PstI C_TGCA'G
Cuts at: 757 1265 1726 2411 757
Size: 508 461 685 3025
Fragments arranged by size:
3025 685 508 461
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 276 406 730 2104 2464 276
Size: 130 324 1374 360 2491
Fragments arranged by size:
2491 1374 360 324 130
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1693 1693
Size: 4679
SacII CC_GC'GG
Cuts at: 1205 1205
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 2702 2912 2702
Size: 210 4469
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 1453 4196 1453
Size: 2743 1936
SexAI A'CCwGG_T
Cuts at: 911 911
Size: 4679
SfcI C'TryA_G
Cuts at: 33 139 753 1261 1722 2407 3375 3566 33
Size: 106 614 508 461 685 968 191 1146
Fragments arranged by size:
1146 968 685 614 508 461 191 106
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 1074 1381 1658 1834 2749 3463 3725 1074
Size: 307 277 176 915 714 262 2028
Fragments arranged by size:
2028 915 714 307 277 262 176
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StyI C'CwwG_G
Cuts at: 107 1813 2167 107
Size: 1706 354 2619
Fragments arranged by size:
2619 1706 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1059 1444 1587 2262 2395 2854 3063 1059
Size: 385 143 675 133 459 209 2675
Fragments arranged by size:
2675 675 459 385 209 143 133
TatI w'GTAC_w
Cuts at: 228 1373 1451 1550 4194 228
Size: 1145 78 99 2644 713
Fragments arranged by size:
2644 1145 713 99 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 4647
Tth111I GACn'n_nGTC
Cuts at: 433 526 1069 2476 433
Size: 93 543 1407 2636
Fragments arranged by size:
2636 1407 543 93
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
XmnI GAAnn'nnTTC
Cuts at: 1360 1360
Size: 4679
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BsaXI BseRI BsiWI
BsmI BspEI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspOMI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI