Restriction map for
pF1KB4688
(Circular) (Six-base) MAPSORT of: pF1KB4688.seq Check: 5906 from: 1 to: 4844
pF1KB4688 4844 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1062 1062
Size: 4844
AccI GT'mk_AC
Cuts at: 252 1882 252
Size: 1630 3214
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 4684 4684
Size: 4844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1523 2669 3101 3454 4116 874
Size: 649 1146 432 353 662 1602
Fragments arranged by size:
1602 1146 662 649 432 353
AflIII A'CryG_T
Cuts at: 2196 3996 4277 2196
Size: 1800 281 2763
Fragments arranged by size:
2763 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1364 1364
Size: 4844
AlwNI CAG_nnn'CTG
Cuts at: 1344 2149 3587 1344
Size: 805 1438 2601
Fragments arranged by size:
2601 1438 805
ApaLI G'TGCA_C
Cuts at: 3682 3682
Size: 4844
ApoI r'AATT_y
Cuts at: 708 1355 1654 1848 4197 4453 708
Size: 647 299 194 2349 256 1099
Fragments arranged by size:
2349 1099 647 299 256 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 447 722 1372 1475 1864 447
Size: 275 650 103 389 3427
Fragments arranged by size:
3427 650 389 275 103
BamHI G'GATC_C
Cuts at: 581 1869 581
Size: 1288 3556
BanI G'GyrC_C
Cuts at: 1108 1368 1495 1860 2274 2522 2557 1108
Size: 260 127 365 414 248 35 3395
Fragments arranged by size:
3395 414 365 260 248 127 35
BanII G_rGCy'C
Cuts at: 599 1858 2888 599
Size: 1259 1030 2555
Fragments arranged by size:
2555 1259 1030
BbeI G_GCGC'C
Cuts at: 2278 2526 2278
Size: 248 4596
BbsI GAAGACnn'nnnn_
Cuts at: 1225 1225
Size: 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 547 581 547
Size: 34 4810
BciVI GTATCCnnnnn_n'
Cuts at: 2742 3798 4844 2742
Size: 1056 1046 2742
Fragments arranged by size:
2742 1056 1046
BclI T'GATC_A
Cuts at: 1694 2364 1694
Size: 670 4174
BglI GCCn_nnn'nGGC
Cuts at: 609 609
Size: 4844
BglII A'GATC_T
Cuts at: 2359 4396 2359
Size: 2037 2807
BlpI GC'TnA_GC
Cuts at: 1956 1956
Size: 4844
Bme1580I G_kGCm'C
Cuts at: 1111 1373 1498 2469 2562 3686 1111
Size: 262 125 971 93 1124 2269
Fragments arranged by size:
2269 1124 971 262 125 93
BmrI ACTGGGnnnn_n'
Cuts at: 2056 2137 2472 4616 2056
Size: 81 335 2144 2284
Fragments arranged by size:
2284 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 4844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1174 634
Size: 540 4304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 865 1189 2020 2899 3613 3911 98
Size: 767 324 831 879 714 298 1031
Fragments arranged by size:
1031 879 831 767 714 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 1507 1563 4548 1507
Size: 56 2985 1803
Fragments arranged by size:
2985 1803 56
BsaAI yAC'GTr
Cuts at: 1548 2197 1548
Size: 649 4195
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 2275 2523 3225 4721 2275
Size: 248 702 1496 2398
Fragments arranged by size:
2398 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2033 2554 3232 3643 3790 4064 2033
Size: 521 678 411 147 274 2813
Fragments arranged by size:
2813 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1003
Size: 30 4814
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 446 862 1018 1228 1668 446
Size: 416 156 210 440 3622
Fragments arranged by size:
3622 440 416 210 156
BseYI C'CCAG_C
Cuts at: 2269 3027 3692 4100 2269
Size: 758 665 408 3013
Fragments arranged by size:
3013 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 323
Size: 4844
BsiEI CG_ry'CG
Cuts at: 104 2386 2432 3662 104
Size: 2282 46 1230 1286
Fragments arranged by size:
2282 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1858 2636 2826 3686 1858
Size: 778 190 860 3016
Fragments arranged by size:
3016 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 599 1111 1373 1498 1858 2469 2562 2636 2826
Size: 512 262 125 360 611 93 74 190
Cuts at: 2826 2888 3686 599
Size: 62 798 1757
Fragments arranged by size:
1757 798 611 512 360 262 190 125
93 74 62
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 238 1062 1894 2108 2410 2791 238
Size: 824 832 214 302 381 2291
Fragments arranged by size:
2291 832 824 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3136 3190 4674 4839 50
Size: 3086 54 1484 165 55
Fragments arranged by size:
3086 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1140 2756 1140
Size: 1616 3228
BsrFI r'CCGG_y
Cuts at: 2842 3023 2842
Size: 181 4663
BssSI C'ACGA_G
Cuts at: 1566 3115 3823 1566
Size: 1549 708 2587
Fragments arranged by size:
2587 1549 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2221 2221
Size: 4844
BstBI TT'CG_AA
Cuts at: 3205 3205
Size: 4844
BstYI r'GATC_y
Cuts at: 1 581 986 1157 1869 2336 2359 2694 2940
Size: 580 405 171 712 467 23 335 246
Cuts at: 2940 3246 3258 3344 4396 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 712 580 467 449 405 335 306
246 171 86 23 12
BtgI C'CryG_G
Cuts at: 1364 1454 1554 1701 1364
Size: 90 100 147 4507
Fragments arranged by size:
4507 147 100 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2794 2975 3138 2794
Size: 181 163 4500
Fragments arranged by size:
4500 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 2087 1091
Size: 996 3848
DraI TTT'AAA
Cuts at: 1523 1843 1523
Size: 320 4524
DrdI GACnn_nn'nnGTC
Cuts at: 2550 3894 2550
Size: 1344 3500
EaeI y'GGCC_r
Cuts at: 951 1124 2429 2603 2994 3021 4366 951
Size: 173 1305 174 391 27 1345 1429
Fragments arranged by size:
1429 1345 1305 391 174 173 27
EagI C'GGCC_G
Cuts at: 2429 2429
Size: 4844
EarI CTCTTCn'nnn_
Cuts at: 191 621 1542 2867 3077 191
Size: 430 921 1325 210 1958
Fragments arranged by size:
1958 1325 921 430 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3052 3794 3940 4654 3052
Size: 742 146 714 3242
Fragments arranged by size:
3242 742 714 146
EcoICRI GAG'CTC
Cuts at: 1856 1856
Size: 4844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 874 1174 1523 2669 3101 3454 4116 634
Size: 240 300 349 1146 432 353 662 1362
Fragments arranged by size:
1362 1146 662 432 353 349 300 240
EcoO109I rG'GnC_Cy
Cuts at: 1983 1983
Size: 4844
EcoRI G'AATT_C
Cuts at: 1355 1848 1355
Size: 493 4351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 514 2863 514
Size: 2349 2495
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 2278 2526 3756 4527 2278
Size: 248 1230 771 2595
Fragments arranged by size:
2595 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 1311 1343 1708 1740 2686 2716 2718
Size: 32 900 32 365 32 946 30 2
Cuts at: 2718 2748 379
Size: 30 2475
Fragments arranged by size:
2475 946 900 365 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 387 1883 387
Size: 1496 3348
KasI G'GCGC_C
Cuts at: 2274 2522 2274
Size: 248 4596
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MfeI C'AATT_G
Cuts at: 1671 1671
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1754 3603 3787 1062
Size: 692 1849 184 2119
Fragments arranged by size:
2119 1849 692 184
MscI TGG'CCA
Cuts at: 953 1126 2605 953
Size: 173 1479 3192
Fragments arranged by size:
3192 1479 173
MslI CAynn'nnrTG
Cuts at: 2833 2960 2833
Size: 127 4717
MspA1I CmG'CkG
Cuts at: 307 1344 1955 2269 2629 3413 3658 307
Size: 1037 611 314 360 784 245 1493
Fragments arranged by size:
1493 1037 784 611 360 314 245
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 4844
NarI GG'CG_CC
Cuts at: 2275 2523 2275
Size: 248 4596
NcoI C'CATG_G
Cuts at: 1364 1554 1701 1364
Size: 190 147 4507
Fragments arranged by size:
4507 190 147
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 4844
NspI r_CATG'y
Cuts at: 1152 1897 2397 4000 1152
Size: 745 500 1603 1996
Fragments arranged by size:
1996 1603 745 500
PciI A'CATG_T
Cuts at: 3996 3996
Size: 4844
PflMI CCAn_nnn'nTGG
Cuts at: 949 1243 1737 949
Size: 294 494 4056
Fragments arranged by size:
4056 494 294
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1297 1329 3290 3322 1297
Size: 32 1961 32 2819
Fragments arranged by size:
2819 1961 32 32
PshAI GACnn'nnGTC
Cuts at: 1818 1818
Size: 4844
PstI C_TGCA'G
Cuts at: 312 590 879 1098 1891 2576 312
Size: 278 289 219 793 685 2580
Fragments arranged by size:
2580 793 685 289 278 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 307 1344 2269 2629 307
Size: 1037 925 360 2522
Fragments arranged by size:
2522 1037 925 360
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1858 1858
Size: 4844
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
SbfI CC_TGCA'GG
Cuts at: 1891 1891
Size: 4844
ScaI AGT'ACT
Cuts at: 4361 4361
Size: 4844
SfcI C'TryA_G
Cuts at: 33 308 329 586 875 1094 1388 1887 2572
Size: 275 21 257 289 219 294 499 685
Cuts at: 2572 3540 3731 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 499 294 289 275 257
219 191 21
SfoI GGC'GCC
Cuts at: 2276 2524 2276
Size: 248 4596
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 724 1866 724
Size: 1142 3702
SmlI C'TyrA_G
Cuts at: 113 844 1204 1999 2914 3628 3890 113
Size: 731 360 795 915 714 262 1067
Fragments arranged by size:
1067 915 795 731 714 360 262
SpeI A'CTAG_T
Cuts at: 67 1294 67
Size: 1227 3617
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
StuI AGG'CCT
Cuts at: 1043 1043
Size: 4844
StyI C'CwwG_G
Cuts at: 761 1161 1364 1448 1554 1701 1978 2332 761
Size: 400 203 84 106 147 277 354 3273
Fragments arranged by size:
3273 400 354 277 203 147 106 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1239 2427 2560 3019 3228 1239
Size: 1188 133 459 209 2855
Fragments arranged by size:
2855 1188 459 209 133
TatI w'GTAC_w
Cuts at: 1019 1786 4359 1019
Size: 767 2573 1504
Fragments arranged by size:
2573 1504 767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4361 4393 4361
Size: 32 4812
Tth111I GACn'n_nGTC
Cuts at: 2641 2641
Size: 4844
XbaI T'CTAG_A
Cuts at: 1875 1875
Size: 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1119 1552 2339 1119
Size: 433 787 3624
Fragments arranged by size:
3624 787 433
XmaI C'CCGG_G
Cuts at: 722 1864 722
Size: 1142 3702
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI