Restriction map for
pF1KB4690
(Circular) (Six-base) MAPSORT of: pF1KB4690.seq Check: 4306 from: 1 to: 5798
pF1KB4690 5798 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 2836 2836
Size: 5798
Acc65I G'GTAC_C
Cuts at: 2099 2814 2099
Size: 715 5083
AclI AA'CG_TT
Cuts at: 5638 5638
Size: 5798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 568 643 964 1247 1930 2618 2670 3623
Size: 381 75 321 283 683 688 52 953
Cuts at: 3623 4055 4802 5070 187
Size: 432 747 268 915
Fragments arranged by size:
953 915 747 688 683 432 381 321
283 268 75 52
AflIII A'CryG_T
Cuts at: 2162 2528 3150 4254 5231 2162
Size: 366 622 1104 977 2729
Fragments arranged by size:
2729 1104 977 622 366
AgeI A'CCGG_T
Cuts at: 4186 4186
Size: 5798
AhdI GACnn_n'nnGTC
Cuts at: 1053 1053
Size: 5798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 710 1654 710
Size: 944 4854
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 818 850 818
Size: 32 5766
AlwNI CAG_nnn'CTG
Cuts at: 1039 1201 1818 2371 2488 3103 4670 1039
Size: 162 617 553 117 615 1567 2167
Fragments arranged by size:
2167 1567 617 615 553 162 117
ApaI G_GGCC'C
Cuts at: 494 793 802 907 2557 494
Size: 299 9 105 1650 3735
Fragments arranged by size:
3735 1650 299 105 9
ApaLI G'TGCA_C
Cuts at: 4568 4568
Size: 5798
ApoI r'AATT_y
Cuts at: 374 483 969 1238 2185 2802 5151 5407 374
Size: 109 486 269 947 617 2349 256 765
Fragments arranged by size:
2349 947 765 617 486 269 256 109
AseI AT'TA_AT
Cuts at: 20 20
Size: 5798
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
AvaI C'yCGr_G
Cuts at: 124 770 2818 124
Size: 646 2048 3104
Fragments arranged by size:
3104 2048 646
BamHI G'GATC_C
Cuts at: 2613 2823 2613
Size: 210 5588
BanI G'GyrC_C
Cuts at: 181 706 2099 2592 2814 3228 3476 3511 181
Size: 525 1393 493 222 414 248 35 2468
Fragments arranged by size:
2468 1393 525 493 414 248 222 35
BanII G_rGCy'C
Cuts at: 231 494 638 793 802 831 907 1147 1250
Size: 263 144 155 9 29 76 240 103
Cuts at: 1250 2557 2812 3842 231
Size: 1307 255 1030 2187
Fragments arranged by size:
2187 1307 1030 263 255 240 155 144
103 76 29 9
BbeI G_GCGC'C
Cuts at: 185 2596 3232 3480 185
Size: 2411 636 248 2503
Fragments arranged by size:
2503 2411 636 248
BbsI GAAGACnn'nnnn_
Cuts at: 1753 2300 1753
Size: 547 5251
BbvCI CC'TCA_GC
Cuts at: 2404 2450 2404
Size: 46 5752
BciVI GTATCCnnnnn_n'
Cuts at: 2051 3696 4457 5798 2051
Size: 1645 761 1341 2051
Fragments arranged by size:
2051 1645 1341 761
BclI T'GATC_A
Cuts at: 3318 3318
Size: 5798
BglI GCCn_nnn'nGGC
Cuts at: 179 214 290 797 2552 2762 179
Size: 35 76 507 1755 210 3215
Fragments arranged by size:
3215 1755 507 210 76 35
BglII A'GATC_T
Cuts at: 1929 3313 5350 1929
Size: 1384 2037 2377
Fragments arranged by size:
2377 2037 1384
BlpI GC'TnA_GC
Cuts at: 557 2702 2741 2910 557
Size: 2145 39 169 3445
Fragments arranged by size:
3445 2145 169 39
Bme1580I G_kGCm'C
Cuts at: 494 709 793 802 907 2557 3423 3516 4572
Size: 215 84 9 105 1650 866 93 1056
Cuts at: 4572 494
Size: 1720
Fragments arranged by size:
1720 1650 1056 866 215 105 93 84
9
BmgBI CAC'GTC
Cuts at: 2163 2163
Size: 5798
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 2150 3010 3091 3426 5570 706
Size: 81 1363 860 81 335 2144 934
Fragments arranged by size:
2144 1363 934 860 335 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1182 4165 1182
Size: 2983 2815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 691 767 887 1366 2111 2372 152
Size: 539 76 120 479 745 261 3578
Fragments arranged by size:
3578 745 539 479 261 120 76
Bpu10I CC'TnA_GC
Cuts at: 1562 1945 2404 2450 2564 1562
Size: 383 459 46 114 4796
Fragments arranged by size:
4796 459 383 114 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 1564 2651 2974 3853 4345 4643 1486
Size: 78 1087 323 879 492 298 2641
Fragments arranged by size:
2641 1087 879 492 323 298 78
BsaI GGTCTCn'nnnn_
Cuts at: 2377 5502 2377
Size: 3125 2673
BsaAI yAC'GTr
Cuts at: 3151 3151
Size: 5798
BsaBI GATnn'nnATC
Cuts at: 6 3312 5349 6
Size: 3306 2037 455
Fragments arranged by size:
3306 2037 455
BsaHI Gr'CG_yC
Cuts at: 182 2593 3229 3477 4179 5675 182
Size: 2411 636 248 702 1496 305
Fragments arranged by size:
2411 1496 702 636 305 248
BsaWI w'CCGG_w
Cuts at: 1079 2987 3508 4186 4460 4607 5018 1079
Size: 1908 521 678 274 147 411 1859
Fragments arranged by size:
1908 1859 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 818 848 2773 2803 818
Size: 30 1925 30 3813
Fragments arranged by size:
3813 1925 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 410 491 550 553 604 749 1057 1399
Size: 145 81 59 3 51 145 308 342
Cuts at: 1399 2437 2656 265
Size: 1038 219 3407
Fragments arranged by size:
3407 1038 342 308 219 145 145 81
59 51 3
BseYI C'CCAG_C
Cuts at: 1115 1194 2114 3223 3981 4558 5054 1115
Size: 79 920 1109 758 577 496 1859
Fragments arranged by size:
1859 1109 920 758 577 496 79
BsiEI CG_ry'CG
Cuts at: 104 310 3340 3386 4594 104
Size: 206 3030 46 1208 1308
Fragments arranged by size:
3030 1308 1208 206 46
BsiHKAI G_wGCw'C
Cuts at: 231 638 1147 1391 2708 2812 3590 3780 4572
Size: 407 509 244 1317 104 778 190 792
Cuts at: 4572 231
Size: 1457
Fragments arranged by size:
1457 1317 792 778 509 407 244 190
104
Bsp1286I G_dGCh'C
Cuts at: 231 494 638 709 793 802 831 907 1147
Size: 263 144 71 84 9 29 76 240
Cuts at: 1147 1250 1391 2557 2708 2812 3423 3516 3590
Size: 103 141 1166 151 104 611 93 74
Cuts at: 3590 3780 3842 4572 231
Size: 190 62 730 1457
Fragments arranged by size:
1457 1166 730 611 263 240 190 151
144 141 104 103 93 84 76 74
71 62 29 9
BspEI T'CCGG_A
Cuts at: 5018 5018
Size: 5798
BspHI T'CATG_A
Cuts at: 4974 4974
Size: 5798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1646 2848 3062 3364 3745 1646
Size: 1202 214 302 381 3699
Fragments arranged by size:
3699 1202 381 302 214
BsrBI CCG'CTC
Cuts at: 50 264 343 543 2151 4090 4144 5628 5793
Size: 214 79 200 1608 1939 54 1484 165
Cuts at: 5793 50
Size: 55
Fragments arranged by size:
1939 1608 1484 214 200 165 79 55
54
BsrDI GCAATG_nn'
Cuts at: 571 3710 571
Size: 3139 2659
BsrFI r'CCGG_y
Cuts at: 309 3796 3977 4186 309
Size: 3487 181 209 1921
Fragments arranged by size:
3487 1921 209 181
BssSI C'ACGA_G
Cuts at: 845 4069 4427 845
Size: 3224 358 2216
Fragments arranged by size:
3224 2216 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3175 3175
Size: 5798
BstBI TT'CG_AA
Cuts at: 4159 4159
Size: 5798
BstXI CCAn_nnnn'nTGG
Cuts at: 1616 2110 1616
Size: 494 5304
BstYI r'GATC_y
Cuts at: 1 911 932 1271 1929 2613 2823 3290 3313
Size: 910 21 339 658 684 210 467 23
Cuts at: 3313 3648 3894 4906 4992 5004 5350 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 910 684 658 467 449 346 339
335 246 210 86 23 21 12
BtgI C'CryG_G
Cuts at: 107 821 1179 1866 1999 2534 2757 107
Size: 714 358 687 133 535 223 3148
Fragments arranged by size:
3148 714 687 535 358 223 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2443 3748 3929 4092 2443
Size: 1305 181 163 4149
Fragments arranged by size:
4149 1305 181 163
BtsI GCAGTG_nn'
Cuts at: 1419 3041 1419
Size: 1622 4176
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 377 412 467 502 377
Size: 35 55 35 5673
Fragments arranged by size:
5673 55 35 35
DraI TTT'AAA
Cuts at: 2797 2797
Size: 5798
DraIII CAC_nnn'GTG
Cuts at: 1545 1545
Size: 5798
DrdI GACnn_nn'nnGTC
Cuts at: 1755 3504 4362 1755
Size: 1749 858 3191
Fragments arranged by size:
3191 1749 858
EaeI y'GGCC_r
Cuts at: 307 2621 3383 3557 3948 3975 5320 307
Size: 2314 762 174 391 27 1345 785
Fragments arranged by size:
2314 1345 785 762 391 174 27
EagI C'GGCC_G
Cuts at: 307 3383 307
Size: 3076 2722
EarI CTCTTCn'nnn_
Cuts at: 956 980 1376 2204 2264 2270 2461 2470 2594
Size: 24 396 828 60 6 191 9 124
Cuts at: 2594 3821 4031 956
Size: 1227 210 2723
Fragments arranged by size:
2723 1227 828 396 210 191 124 60
24 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 413 4006 4316 4462 5608 413
Size: 3593 310 146 1146 603
Fragments arranged by size:
3593 1146 603 310 146
EcoICRI GAG'CTC
Cuts at: 229 636 1145 2810 229
Size: 407 509 1665 3217
Fragments arranged by size:
3217 1665 509 407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 187 568 643 691 767 887 964 1247
Size: 35 381 75 48 76 120 77 283
Cuts at: 1247 1366 1930 2111 2372 2618 2670 3623 4055
Size: 119 564 181 261 246 52 953 432
Cuts at: 4055 4802 5070 152
Size: 747 268 880
Fragments arranged by size:
953 880 747 564 432 381 283 268
261 246 181 120 119 77 76 75
52 48 35
EcoNI CCTnn'n_nnAGG
Cuts at: 443 443
Size: 5798
EcoO109I rG'GnC_Cy
Cuts at: 490 491 766 799 904 1557 1722 1872 1989
Size: 1 275 33 105 653 165 150 117
Cuts at: 1989 2553 2937 490
Size: 564 384 3351
Fragments arranged by size:
3351 653 564 384 275 165 150 117
105 33 1
EcoRI G'AATT_C
Cuts at: 374 969 2802 374
Size: 595 1833 3370
Fragments arranged by size:
3370 1833 595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1290 1686 2700 3817 1290
Size: 396 1014 1117 3271
Fragments arranged by size:
3271 1117 1014 396
FspI TGC'GCA
Cuts at: 754 3579 754
Size: 2825 2973
FspAI rTGC'GCAy
Cuts at: 754 754
Size: 5798
HaeII r_GCGC'y
Cuts at: 185 2590 2596 3232 3480 4502 5481 185
Size: 2405 6 636 248 1022 979 502
Fragments arranged by size:
2405 1022 979 636 502 248 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 213 218 245 250 880 912 957
Size: 32 76 5 27 5 630 32 45
Cuts at: 957 989 994 1026 1039 1071 2242 2274 3640
Size: 32 5 32 13 32 1171 32 1366
Cuts at: 3640 3670 3672 3702 105
Size: 30 2 30 2201
Fragments arranged by size:
2201 1366 1171 630 76 45 32 32
32 32 32 32 30 30 27 13
5 5 5 2
HincII GTy'rAC
Cuts at: 2837 2837
Size: 5798
KasI G'GCGC_C
Cuts at: 181 2592 3228 3476 181
Size: 2411 636 248 2503
Fragments arranged by size:
2503 2411 636 248
KpnI G_GTAC'C
Cuts at: 2103 2818 2103
Size: 715 5083
MluI A'CGCG_T
Cuts at: 5231 5231
Size: 5798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2472 2585 4469 4653 2472
Size: 113 1884 184 3617
Fragments arranged by size:
3617 1884 184 113
MscI TGG'CCA
Cuts at: 2623 3559 2623
Size: 936 4862
MslI CAynn'nnrTG
Cuts at: 2108 2522 2539 3787 3914 2108
Size: 414 17 1248 127 3992
Fragments arranged by size:
3992 1248 414 127 17
MspA1I CmG'CkG
Cuts at: 150 661 942 1119 1201 1509 2909 3223 3583
Size: 511 281 177 82 308 1400 314 360
Cuts at: 3583 4596 4841 150
Size: 1013 245 1107
Fragments arranged by size:
1400 1107 1013 511 360 314 308 281
245 177 82
NaeI GCC'GGC
Cuts at: 3979 3979
Size: 5798
NarI GG'CG_CC
Cuts at: 182 2593 3229 3477 182
Size: 2411 636 248 2503
Fragments arranged by size:
2503 2411 636 248
NcoI C'CATG_G
Cuts at: 107 821 1866 1999 107
Size: 714 1045 133 3906
Fragments arranged by size:
3906 1045 714 133
NgoMIV G'CCGG_C
Cuts at: 3977 3977
Size: 5798
NruI TCG'CGA
Cuts at: 248 248
Size: 5798
NspI r_CATG'y
Cuts at: 2532 2650 2851 3351 4258 2532
Size: 118 201 500 907 4072
Fragments arranged by size:
4072 907 500 201 118
PasI CC'CwG_GG
Cuts at: 1017 2059 1017
Size: 1042 4756
PciI A'CATG_T
Cuts at: 2528 4254 2528
Size: 1726 4072
PflMI CCAn_nnn'nTGG
Cuts at: 451 924 451
Size: 473 5325
PfoI T'CCnGG_A
Cuts at: 1706 2349 1706
Size: 643 5155
PmeI GTTT'AAAC
Cuts at: 2797 2797
Size: 5798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 620 652 818 850 1760 1792 4937 4969 620
Size: 32 166 32 910 32 3145 32 1449
Fragments arranged by size:
3145 1449 910 166 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 766 1557 1872 1989 766
Size: 791 315 117 4575
Fragments arranged by size:
4575 791 315 117
PspOMI G'GGCC_C
Cuts at: 490 789 798 903 2553 490
Size: 299 9 105 1650 3735
Fragments arranged by size:
3735 1650 299 105 9
PspXI vC'TCGA_Gb
Cuts at: 770 770
Size: 5798
PstI C_TGCA'G
Cuts at: 272 416 941 1359 1643 1730 2331 2845 3530
Size: 144 525 418 284 87 601 514 685
Cuts at: 3530 272
Size: 2540
Fragments arranged by size:
2540 685 601 525 514 418 284 144
87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5798
PvuII CAG'CTG
Cuts at: 150 661 942 1201 1509 3223 3583 150
Size: 511 281 259 308 1714 360 2365
Fragments arranged by size:
2365 1714 511 360 308 281 259
RsrII CG'GwC_CG
Cuts at: 3993 3993
Size: 5798
SacI G_AGCT'C
Cuts at: 231 638 1147 2812 231
Size: 407 509 1665 3217
Fragments arranged by size:
3217 1665 509 407
SalI G'TCGA_C
Cuts at: 2835 2835
Size: 5798
SapI GCTCTTCn'nnn_
Cuts at: 2461 3821 4031 2461
Size: 1360 210 4228
Fragments arranged by size:
4228 1360 210
SbfI CC_TGCA'GG
Cuts at: 1643 1730 2845 1643
Size: 87 1115 4596
Fragments arranged by size:
4596 1115 87
ScaI AGT'ACT
Cuts at: 5315 5315
Size: 5798
SfcI C'TryA_G
Cuts at: 33 268 412 809 937 1355 1639 1726 2327
Size: 235 144 397 128 418 284 87 601
Cuts at: 2327 2841 3526 4519 4710 33
Size: 514 685 993 191 1121
Fragments arranged by size:
1121 993 685 601 514 418 397 284
235 191 144 128 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 797 797
Size: 5798
SfoI GGC'GCC
Cuts at: 183 2594 3230 3478 183
Size: 2411 636 248 2503
Fragments arranged by size:
2503 2411 636 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
SmaI CCC'GGG
Cuts at: 2820 2820
Size: 5798
SmlI C'TyrA_G
Cuts at: 770 1501 1579 2666 2953 3868 4360 4622 770
Size: 731 78 1087 287 915 492 262 1946
Fragments arranged by size:
1946 1087 915 731 492 287 262 78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5798
SphI G_CATG'C
Cuts at: 2851 3351 2851
Size: 500 5298
StuI AGG'CCT
Cuts at: 449 449
Size: 5798
StyI C'CwwG_G
Cuts at: 107 450 456 821 1866 1955 1999 2227 2932
Size: 343 6 365 1045 89 44 228 705
Cuts at: 2932 3286 107
Size: 354 2619
Fragments arranged by size:
2619 1045 705 365 354 343 228 89
44 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 491 502 3381 3514 3973 4182 491
Size: 11 2879 133 459 209 2107
Fragments arranged by size:
2879 2107 459 209 133 11
TatI w'GTAC_w
Cuts at: 1877 5313 1877
Size: 3436 2362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5315 5347 5315
Size: 32 5766
Tth111I GACn'n_nGTC
Cuts at: 3595 3595
Size: 5798
XbaI T'CTAG_A
Cuts at: 2829 2829
Size: 5798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2755 3293 2755
Size: 538 5260
XhoI C'TCGA_G
Cuts at: 770 770
Size: 5798
XmaI C'CCGG_G
Cuts at: 2818 2818
Size: 5798
XmnI GAAnn'nnTTC
Cuts at: 1483 1894 1483
Size: 411 5387
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BaeI
BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PmlI
PshAI PsiI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI ZraI