(Circular) (Six-base) MAPSORT of: pF1KB4690.seq Check: 4306 from: 1 to: 5798 pF1KB4690 5798 bp With 182 enzymes: SgfI * September 18, 2009 17:00 .. AccI GT'mk_AC Cuts at: 2836 2836 Size: 5798 Acc65I G'GTAC_C Cuts at: 2099 2814 2099 Size: 715 5083 AclI AA'CG_TT Cuts at: 5638 5638 Size: 5798 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 187 568 643 964 1247 1930 2618 2670 3623 Size: 381 75 321 283 683 688 52 953 Cuts at: 3623 4055 4802 5070 187 Size: 432 747 268 915 Fragments arranged by size: 953 915 747 688 683 432 381 321 283 268 75 52 AflIII A'CryG_T Cuts at: 2162 2528 3150 4254 5231 2162 Size: 366 622 1104 977 2729 Fragments arranged by size: 2729 1104 977 622 366 AgeI A'CCGG_T Cuts at: 4186 4186 Size: 5798 AhdI GACnn_n'nnGTC Cuts at: 1053 1053 Size: 5798 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 710 1654 710 Size: 944 4854 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 818 850 818 Size: 32 5766 AlwNI CAG_nnn'CTG Cuts at: 1039 1201 1818 2371 2488 3103 4670 1039 Size: 162 617 553 117 615 1567 2167 Fragments arranged by size: 2167 1567 617 615 553 162 117 ApaI G_GGCC'C Cuts at: 494 793 802 907 2557 494 Size: 299 9 105 1650 3735 Fragments arranged by size: 3735 1650 299 105 9 ApaLI G'TGCA_C Cuts at: 4568 4568 Size: 5798 ApoI r'AATT_y Cuts at: 374 483 969 1238 2185 2802 5151 5407 374 Size: 109 486 269 947 617 2349 256 765 Fragments arranged by size: 2349 947 765 617 486 269 256 109 AseI AT'TA_AT Cuts at: 20 20 Size: 5798 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5798 AvaI C'yCGr_G Cuts at: 124 770 2818 124 Size: 646 2048 3104 Fragments arranged by size: 3104 2048 646 BamHI G'GATC_C Cuts at: 2613 2823 2613 Size: 210 5588 BanI G'GyrC_C Cuts at: 181 706 2099 2592 2814 3228 3476 3511 181 Size: 525 1393 493 222 414 248 35 2468 Fragments arranged by size: 2468 1393 525 493 414 248 222 35 BanII G_rGCy'C Cuts at: 231 494 638 793 802 831 907 1147 1250 Size: 263 144 155 9 29 76 240 103 Cuts at: 1250 2557 2812 3842 231 Size: 1307 255 1030 2187 Fragments arranged by size: 2187 1307 1030 263 255 240 155 144 103 76 29 9 BbeI G_GCGC'C Cuts at: 185 2596 3232 3480 185 Size: 2411 636 248 2503 Fragments arranged by size: 2503 2411 636 248 BbsI GAAGACnn'nnnn_ Cuts at: 1753 2300 1753 Size: 547 5251 BbvCI CC'TCA_GC Cuts at: 2404 2450 2404 Size: 46 5752 BciVI GTATCCnnnnn_n' Cuts at: 2051 3696 4457 5798 2051 Size: 1645 761 1341 2051 Fragments arranged by size: 2051 1645 1341 761 BclI T'GATC_A Cuts at: 3318 3318 Size: 5798 BglI GCCn_nnn'nGGC Cuts at: 179 214 290 797 2552 2762 179 Size: 35 76 507 1755 210 3215 Fragments arranged by size: 3215 1755 507 210 76 35 BglII A'GATC_T Cuts at: 1929 3313 5350 1929 Size: 1384 2037 2377 Fragments arranged by size: 2377 2037 1384 BlpI GC'TnA_GC Cuts at: 557 2702 2741 2910 557 Size: 2145 39 169 3445 Fragments arranged by size: 3445 2145 169 39 Bme1580I G_kGCm'C Cuts at: 494 709 793 802 907 2557 3423 3516 4572 Size: 215 84 9 105 1650 866 93 1056 Cuts at: 4572 494 Size: 1720 Fragments arranged by size: 1720 1650 1056 866 215 105 93 84 9 BmgBI CAC'GTC Cuts at: 2163 2163 Size: 5798 BmrI ACTGGGnnnn_n' Cuts at: 706 787 2150 3010 3091 3426 5570 706 Size: 81 1363 860 81 335 2144 934 Fragments arranged by size: 2144 1363 934 860 335 81 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1182 4165 1182 Size: 2983 2815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 152 691 767 887 1366 2111 2372 152 Size: 539 76 120 479 745 261 3578 Fragments arranged by size: 3578 745 539 479 261 120 76 Bpu10I CC'TnA_GC Cuts at: 1562 1945 2404 2450 2564 1562 Size: 383 459 46 114 4796 Fragments arranged by size: 4796 459 383 114 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1486 1564 2651 2974 3853 4345 4643 1486 Size: 78 1087 323 879 492 298 2641 Fragments arranged by size: 2641 1087 879 492 323 298 78 BsaI GGTCTCn'nnnn_ Cuts at: 2377 5502 2377 Size: 3125 2673 BsaAI yAC'GTr Cuts at: 3151 3151 Size: 5798 BsaBI GATnn'nnATC Cuts at: 6 3312 5349 6 Size: 3306 2037 455 Fragments arranged by size: 3306 2037 455 BsaHI Gr'CG_yC Cuts at: 182 2593 3229 3477 4179 5675 182 Size: 2411 636 248 702 1496 305 Fragments arranged by size: 2411 1496 702 636 305 248 BsaWI w'CCGG_w Cuts at: 1079 2987 3508 4186 4460 4607 5018 1079 Size: 1908 521 678 274 147 411 1859 Fragments arranged by size: 1908 1859 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 818 848 2773 2803 818 Size: 30 1925 30 3813 Fragments arranged by size: 3813 1925 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 265 410 491 550 553 604 749 1057 1399 Size: 145 81 59 3 51 145 308 342 Cuts at: 1399 2437 2656 265 Size: 1038 219 3407 Fragments arranged by size: 3407 1038 342 308 219 145 145 81 59 51 3 BseYI C'CCAG_C Cuts at: 1115 1194 2114 3223 3981 4558 5054 1115 Size: 79 920 1109 758 577 496 1859 Fragments arranged by size: 1859 1109 920 758 577 496 79 BsiEI CG_ry'CG Cuts at: 104 310 3340 3386 4594 104 Size: 206 3030 46 1208 1308 Fragments arranged by size: 3030 1308 1208 206 46 BsiHKAI G_wGCw'C Cuts at: 231 638 1147 1391 2708 2812 3590 3780 4572 Size: 407 509 244 1317 104 778 190 792 Cuts at: 4572 231 Size: 1457 Fragments arranged by size: 1457 1317 792 778 509 407 244 190 104 Bsp1286I G_dGCh'C Cuts at: 231 494 638 709 793 802 831 907 1147 Size: 263 144 71 84 9 29 76 240 Cuts at: 1147 1250 1391 2557 2708 2812 3423 3516 3590 Size: 103 141 1166 151 104 611 93 74 Cuts at: 3590 3780 3842 4572 231 Size: 190 62 730 1457 Fragments arranged by size: 1457 1166 730 611 263 240 190 151 144 141 104 103 93 84 76 74 71 62 29 9 BspEI T'CCGG_A Cuts at: 5018 5018 Size: 5798 BspHI T'CATG_A Cuts at: 4974 4974 Size: 5798 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1646 2848 3062 3364 3745 1646 Size: 1202 214 302 381 3699 Fragments arranged by size: 3699 1202 381 302 214 BsrBI CCG'CTC Cuts at: 50 264 343 543 2151 4090 4144 5628 5793 Size: 214 79 200 1608 1939 54 1484 165 Cuts at: 5793 50 Size: 55 Fragments arranged by size: 1939 1608 1484 214 200 165 79 55 54 BsrDI GCAATG_nn' Cuts at: 571 3710 571 Size: 3139 2659 BsrFI r'CCGG_y Cuts at: 309 3796 3977 4186 309 Size: 3487 181 209 1921 Fragments arranged by size: 3487 1921 209 181 BssSI C'ACGA_G Cuts at: 845 4069 4427 845 Size: 3224 358 2216 Fragments arranged by size: 3224 2216 358 BstAPI GCAn_nnn'nTGC Cuts at: 3175 3175 Size: 5798 BstBI TT'CG_AA Cuts at: 4159 4159 Size: 5798 BstXI CCAn_nnnn'nTGG Cuts at: 1616 2110 1616 Size: 494 5304 BstYI r'GATC_y Cuts at: 1 911 932 1271 1929 2613 2823 3290 3313 Size: 910 21 339 658 684 210 467 23 Cuts at: 3313 3648 3894 4906 4992 5004 5350 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 910 684 658 467 449 346 339 335 246 210 86 23 21 12 BtgI C'CryG_G Cuts at: 107 821 1179 1866 1999 2534 2757 107 Size: 714 358 687 133 535 223 3148 Fragments arranged by size: 3148 714 687 535 358 223 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2443 3748 3929 4092 2443 Size: 1305 181 163 4149 Fragments arranged by size: 4149 1305 181 163 BtsI GCAGTG_nn' Cuts at: 1419 3041 1419 Size: 1622 4176 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 377 412 467 502 377 Size: 35 55 35 5673 Fragments arranged by size: 5673 55 35 35 DraI TTT'AAA Cuts at: 2797 2797 Size: 5798 DraIII CAC_nnn'GTG Cuts at: 1545 1545 Size: 5798 DrdI GACnn_nn'nnGTC Cuts at: 1755 3504 4362 1755 Size: 1749 858 3191 Fragments arranged by size: 3191 1749 858 EaeI y'GGCC_r Cuts at: 307 2621 3383 3557 3948 3975 5320 307 Size: 2314 762 174 391 27 1345 785 Fragments arranged by size: 2314 1345 785 762 391 174 27 EagI C'GGCC_G Cuts at: 307 3383 307 Size: 3076 2722 EarI CTCTTCn'nnn_ Cuts at: 956 980 1376 2204 2264 2270 2461 2470 2594 Size: 24 396 828 60 6 191 9 124 Cuts at: 2594 3821 4031 956 Size: 1227 210 2723 Fragments arranged by size: 2723 1227 828 396 210 191 124 60 24 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 413 4006 4316 4462 5608 413 Size: 3593 310 146 1146 603 Fragments arranged by size: 3593 1146 603 310 146 EcoICRI GAG'CTC Cuts at: 229 636 1145 2810 229 Size: 407 509 1665 3217 Fragments arranged by size: 3217 1665 509 407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 152 187 568 643 691 767 887 964 1247 Size: 35 381 75 48 76 120 77 283 Cuts at: 1247 1366 1930 2111 2372 2618 2670 3623 4055 Size: 119 564 181 261 246 52 953 432 Cuts at: 4055 4802 5070 152 Size: 747 268 880 Fragments arranged by size: 953 880 747 564 432 381 283 268 261 246 181 120 119 77 76 75 52 48 35 EcoNI CCTnn'n_nnAGG Cuts at: 443 443 Size: 5798 EcoO109I rG'GnC_Cy Cuts at: 490 491 766 799 904 1557 1722 1872 1989 Size: 1 275 33 105 653 165 150 117 Cuts at: 1989 2553 2937 490 Size: 564 384 3351 Fragments arranged by size: 3351 653 564 384 275 165 150 117 105 33 1 EcoRI G'AATT_C Cuts at: 374 969 2802 374 Size: 595 1833 3370 Fragments arranged by size: 3370 1833 595 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1290 1686 2700 3817 1290 Size: 396 1014 1117 3271 Fragments arranged by size: 3271 1117 1014 396 FspI TGC'GCA Cuts at: 754 3579 754 Size: 2825 2973 FspAI rTGC'GCAy Cuts at: 754 754 Size: 5798 HaeII r_GCGC'y Cuts at: 185 2590 2596 3232 3480 4502 5481 185 Size: 2405 6 636 248 1022 979 502 Fragments arranged by size: 2405 1022 979 636 502 248 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 105 137 213 218 245 250 880 912 957 Size: 32 76 5 27 5 630 32 45 Cuts at: 957 989 994 1026 1039 1071 2242 2274 3640 Size: 32 5 32 13 32 1171 32 1366 Cuts at: 3640 3670 3672 3702 105 Size: 30 2 30 2201 Fragments arranged by size: 2201 1366 1171 630 76 45 32 32 32 32 32 32 30 30 27 13 5 5 5 2 HincII GTy'rAC Cuts at: 2837 2837 Size: 5798 KasI G'GCGC_C Cuts at: 181 2592 3228 3476 181 Size: 2411 636 248 2503 Fragments arranged by size: 2503 2411 636 248 KpnI G_GTAC'C Cuts at: 2103 2818 2103 Size: 715 5083 MluI A'CGCG_T Cuts at: 5231 5231 Size: 5798 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2472 2585 4469 4653 2472 Size: 113 1884 184 3617 Fragments arranged by size: 3617 1884 184 113 MscI TGG'CCA Cuts at: 2623 3559 2623 Size: 936 4862 MslI CAynn'nnrTG Cuts at: 2108 2522 2539 3787 3914 2108 Size: 414 17 1248 127 3992 Fragments arranged by size: 3992 1248 414 127 17 MspA1I CmG'CkG Cuts at: 150 661 942 1119 1201 1509 2909 3223 3583 Size: 511 281 177 82 308 1400 314 360 Cuts at: 3583 4596 4841 150 Size: 1013 245 1107 Fragments arranged by size: 1400 1107 1013 511 360 314 308 281 245 177 82 NaeI GCC'GGC Cuts at: 3979 3979 Size: 5798 NarI GG'CG_CC Cuts at: 182 2593 3229 3477 182 Size: 2411 636 248 2503 Fragments arranged by size: 2503 2411 636 248 NcoI C'CATG_G Cuts at: 107 821 1866 1999 107 Size: 714 1045 133 3906 Fragments arranged by size: 3906 1045 714 133 NgoMIV G'CCGG_C Cuts at: 3977 3977 Size: 5798 NruI TCG'CGA Cuts at: 248 248 Size: 5798 NspI r_CATG'y Cuts at: 2532 2650 2851 3351 4258 2532 Size: 118 201 500 907 4072 Fragments arranged by size: 4072 907 500 201 118 PasI CC'CwG_GG Cuts at: 1017 2059 1017 Size: 1042 4756 PciI A'CATG_T Cuts at: 2528 4254 2528 Size: 1726 4072 PflMI CCAn_nnn'nTGG Cuts at: 451 924 451 Size: 473 5325 PfoI T'CCnGG_A Cuts at: 1706 2349 1706 Size: 643 5155 PmeI GTTT'AAAC Cuts at: 2797 2797 Size: 5798 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 620 652 818 850 1760 1792 4937 4969 620 Size: 32 166 32 910 32 3145 32 1449 Fragments arranged by size: 3145 1449 910 166 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 766 1557 1872 1989 766 Size: 791 315 117 4575 Fragments arranged by size: 4575 791 315 117 PspOMI G'GGCC_C Cuts at: 490 789 798 903 2553 490 Size: 299 9 105 1650 3735 Fragments arranged by size: 3735 1650 299 105 9 PspXI vC'TCGA_Gb Cuts at: 770 770 Size: 5798 PstI C_TGCA'G Cuts at: 272 416 941 1359 1643 1730 2331 2845 3530 Size: 144 525 418 284 87 601 514 685 Cuts at: 3530 272 Size: 2540 Fragments arranged by size: 2540 685 601 525 514 418 284 144 87 PvuI CG_AT'CG Cuts at: 104 104 Size: 5798 PvuII CAG'CTG Cuts at: 150 661 942 1201 1509 3223 3583 150 Size: 511 281 259 308 1714 360 2365 Fragments arranged by size: 2365 1714 511 360 308 281 259 RsrII CG'GwC_CG Cuts at: 3993 3993 Size: 5798 SacI G_AGCT'C Cuts at: 231 638 1147 2812 231 Size: 407 509 1665 3217 Fragments arranged by size: 3217 1665 509 407 SalI G'TCGA_C Cuts at: 2835 2835 Size: 5798 SapI GCTCTTCn'nnn_ Cuts at: 2461 3821 4031 2461 Size: 1360 210 4228 Fragments arranged by size: 4228 1360 210 SbfI CC_TGCA'GG Cuts at: 1643 1730 2845 1643 Size: 87 1115 4596 Fragments arranged by size: 4596 1115 87 ScaI AGT'ACT Cuts at: 5315 5315 Size: 5798 SfcI C'TryA_G Cuts at: 33 268 412 809 937 1355 1639 1726 2327 Size: 235 144 397 128 418 284 87 601 Cuts at: 2327 2841 3526 4519 4710 33 Size: 514 685 993 191 1121 Fragments arranged by size: 1121 993 685 601 514 418 397 284 235 191 144 128 87 SfiI GGCCn_nnn'nGGCC Cuts at: 797 797 Size: 5798 SfoI GGC'GCC Cuts at: 183 2594 3230 3478 183 Size: 2411 636 248 2503 Fragments arranged by size: 2503 2411 636 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5798 SmaI CCC'GGG Cuts at: 2820 2820 Size: 5798 SmlI C'TyrA_G Cuts at: 770 1501 1579 2666 2953 3868 4360 4622 770 Size: 731 78 1087 287 915 492 262 1946 Fragments arranged by size: 1946 1087 915 731 492 287 262 78 SpeI A'CTAG_T Cuts at: 67 67 Size: 5798 SphI G_CATG'C Cuts at: 2851 3351 2851 Size: 500 5298 StuI AGG'CCT Cuts at: 449 449 Size: 5798 StyI C'CwwG_G Cuts at: 107 450 456 821 1866 1955 1999 2227 2932 Size: 343 6 365 1045 89 44 228 705 Cuts at: 2932 3286 107 Size: 354 2619 Fragments arranged by size: 2619 1045 705 365 354 343 228 89 44 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 491 502 3381 3514 3973 4182 491 Size: 11 2879 133 459 209 2107 Fragments arranged by size: 2879 2107 459 209 133 11 TatI w'GTAC_w Cuts at: 1877 5313 1877 Size: 3436 2362 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5315 5347 5315 Size: 32 5766 Tth111I GACn'n_nGTC Cuts at: 3595 3595 Size: 5798 XbaI T'CTAG_A Cuts at: 2829 2829 Size: 5798 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2755 3293 2755 Size: 538 5260 XhoI C'TCGA_G Cuts at: 770 770 Size: 5798 XmaI C'CCGG_G Cuts at: 2818 2818 Size: 5798 XmnI GAAnn'nnTTC Cuts at: 1483 1894 1483 Size: 411 5387 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI AscI AvrII BaeI BcgI BfrBI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI ZraI