Restriction map for
pF1KB4698
(Circular) (Six-base) MAPSORT of: pF1KB4698.seq Check: 7517 from: 1 to: 5762
pF1KB4698 5762 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 767 991 2800 767
Size: 224 1809 3729
Fragments arranged by size:
3729 1809 224
Acc65I G'GTAC_C
Cuts at: 803 2778 803
Size: 1975 3787
AclI AA'CG_TT
Cuts at: 2543 5602 2543
Size: 3059 2703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 3587 4019 4372 5034 1052
Size: 2535 432 353 662 1780
Fragments arranged by size:
2535 1780 662 432 353
AfeI AGC'GCT
Cuts at: 1429 1429
Size: 5762
AflIII A'CryG_T
Cuts at: 792 3114 4914 5195 792
Size: 2322 1800 281 1359
Fragments arranged by size:
2322 1800 1359 281
AhdI GACnn_n'nnGTC
Cuts at: 390 390
Size: 5762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 650 650
Size: 5762
AlwNI CAG_nnn'CTG
Cuts at: 3067 4505 3067
Size: 1438 4324
ApaI G_GGCC'C
Cuts at: 1149 1256 1149
Size: 107 5655
ApaLI G'TGCA_C
Cuts at: 4600 4600
Size: 5762
ApoI r'AATT_y
Cuts at: 922 1544 1879 2691 2700 2766 5115 5371 922
Size: 622 335 812 9 66 2349 256 1313
Fragments arranged by size:
2349 1313 812 622 335 256 66 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 5762
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5762
AvaI C'yCGr_G
Cuts at: 1663 2782 1663
Size: 1119 4643
AvrII C'CTAG_G
Cuts at: 208 208
Size: 5762
BamHI G'GATC_C
Cuts at: 2386 2787 2386
Size: 401 5361
BanI G'GyrC_C
Cuts at: 214 803 1915 2778 3192 3440 3475 214
Size: 589 1112 863 414 248 35 2501
Fragments arranged by size:
2501 1112 863 589 414 248 35
BanII G_rGCy'C
Cuts at: 513 972 1149 1241 1256 1313 2207 2776 3806
Size: 459 177 92 15 57 894 569 1030
Cuts at: 3806 513
Size: 2469
Fragments arranged by size:
2469 1030 894 569 459 177 92 57
15
BbeI G_GCGC'C
Cuts at: 218 3196 3444 218
Size: 2978 248 2536
Fragments arranged by size:
2978 2536 248
BbvCI CC'TCA_GC
Cuts at: 2078 2078
Size: 5762
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5728
BciVI GTATCCnnnnn_n'
Cuts at: 3660 4716 5762 3660
Size: 1056 1046 3660
Fragments arranged by size:
3660 1056 1046
BclI T'GATC_A
Cuts at: 1679 2000 3282 1679
Size: 321 1282 4159
Fragments arranged by size:
4159 1282 321
BfrBI ATG'CAT
Cuts at: 123 123
Size: 5762
BglII A'GATC_T
Cuts at: 699 1742 2152 3277 5314 699
Size: 1043 410 1125 2037 1147
Fragments arranged by size:
2037 1147 1125 1043 410
BlpI GC'TnA_GC
Cuts at: 2874 2874
Size: 5762
Bme1580I G_kGCm'C
Cuts at: 1118 1149 1256 2529 3387 3480 4604 1118
Size: 31 107 1273 858 93 1124 2276
Fragments arranged by size:
2276 1273 1124 858 107 93 31
BmgBI CAC'GTC
Cuts at: 432 432
Size: 5762
BmrI ACTGGGnnnn_n'
Cuts at: 339 2398 2974 3055 3390 5534 339
Size: 2059 576 81 335 2144 567
Fragments arranged by size:
2144 2059 576 567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4129 4129
Size: 5762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 986 1293 2570 724
Size: 262 307 1277 3916
Fragments arranged by size:
3916 1277 307 262
Bpu10I CC'TnA_GC
Cuts at: 517 2078 517
Size: 1561 4201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2938 3817 4531 4829 1360
Size: 1578 879 714 298 2293
Fragments arranged by size:
2293 1578 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 486 836 1177 5466 486
Size: 350 341 4289 782
Fragments arranged by size:
4289 782 350 341
BsaAI yAC'GTr
Cuts at: 3115 3115
Size: 5762
BsaBI GATnn'nnATC
Cuts at: 6 1672 2005 3276 5313 6
Size: 1666 333 1271 2037 455
Fragments arranged by size:
2037 1666 1271 455 333
BsaHI Gr'CG_yC
Cuts at: 215 2441 3193 3441 4143 5639 215
Size: 2226 752 248 702 1496 338
Fragments arranged by size:
2226 1496 752 702 338 248
BsaWI w'CCGG_w
Cuts at: 2951 3472 4150 4561 4708 4982 2951
Size: 521 678 411 147 274 3731
Fragments arranged by size:
3731 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1556 1586 2113 2143 1556
Size: 30 527 30 5175
Fragments arranged by size:
5175 527 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 371 1846 2001 371
Size: 1475 155 4132
Fragments arranged by size:
4132 1475 155
BseYI C'CCAG_C
Cuts at: 448 1107 3187 3945 4610 5018 448
Size: 659 2080 758 665 408 1192
Fragments arranged by size:
2080 1192 758 665 659 408
BsiEI CG_ry'CG
Cuts at: 104 2231 3304 3350 4580 104
Size: 2127 1073 46 1230 1286
Fragments arranged by size:
2127 1286 1230 1073 46
BsiHKAI G_wGCw'C
Cuts at: 513 972 1241 1313 2776 3554 3744 4604 513
Size: 459 269 72 1463 778 190 860 1671
Fragments arranged by size:
1671 1463 860 778 459 269 190 72
BsmI GAATG_Cn'
Cuts at: 1089 2664 1089
Size: 1575 4187
BsmBI CGTCTCn'nnnn_
Cuts at: 438 438
Size: 5762
Bsp1286I G_dGCh'C
Cuts at: 513 972 1118 1149 1241 1256 1313 2207 2529
Size: 459 146 31 92 15 57 894 322
Cuts at: 2529 2776 3387 3480 3554 3744 3806 4604 513
Size: 247 611 93 74 190 62 798 1671
Fragments arranged by size:
1671 894 798 611 459 322 247 190
146 93 92 74 62 57 31 15
BspHI T'CATG_A
Cuts at: 1404 1655 1676 4194 1404
Size: 251 21 2518 2972
Fragments arranged by size:
2972 2518 251 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2812 3026 3328 3709 2812
Size: 214 302 381 4865
Fragments arranged by size:
4865 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4054 4108 5592 5757 50
Size: 4004 54 1484 165 55
Fragments arranged by size:
4004 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 815 1428 2594 3674 815
Size: 613 1166 1080 2903
Fragments arranged by size:
2903 1166 1080 613
BsrFI r'CCGG_y
Cuts at: 3760 3941 3760
Size: 181 5581
BssSI C'ACGA_G
Cuts at: 4033 4741 4033
Size: 708 5054
BstAPI GCAn_nnn'nTGC
Cuts at: 597 2659 3139 597
Size: 2062 480 3220
Fragments arranged by size:
3220 2062 480
BstBI TT'CG_AA
Cuts at: 4123 4123
Size: 5762
BstXI CCAn_nnnn'nTGG
Cuts at: 1371 1371
Size: 5762
BstYI r'GATC_y
Cuts at: 1 699 1742 1951 2082 2152 2386 2787 3254
Size: 698 1043 209 131 70 234 401 467
Cuts at: 3254 3277 3612 3858 4164 4176 4262 5314 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1043 698 467 449 401 335 306
246 234 209 131 86 70 23 12
BstZ17I GTA'TAC
Cuts at: 992 992
Size: 5762
Bsu36I CC'TnA_GG
Cuts at: 1048 1048
Size: 5762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2355 3712 3893 4056 2355
Size: 1357 181 163 4061
Fragments arranged by size:
4061 1357 181 163
BtsI GCAGTG_nn'
Cuts at: 586 1118 2171 3005 586
Size: 532 1053 834 3343
Fragments arranged by size:
3343 1053 834 532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1458 1493 2576 2611 1458
Size: 35 1083 35 4609
Fragments arranged by size:
4609 1083 35 35
DraI TTT'AAA
Cuts at: 2761 2761
Size: 5762
DrdI GACnn_nn'nnGTC
Cuts at: 3468 4812 3468
Size: 1344 4418
EaeI y'GGCC_r
Cuts at: 2228 3347 3521 3912 3939 5284 2228
Size: 1119 174 391 27 1345 2706
Fragments arranged by size:
2706 1345 1119 391 174 27
EagI C'GGCC_G
Cuts at: 2228 3347 2228
Size: 1119 4643
EarI CTCTTCn'nnn_
Cuts at: 180 1622 1842 3785 3995 180
Size: 1442 220 1943 210 1947
Fragments arranged by size:
1947 1943 1442 220 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3970 4712 4858 5572 3970
Size: 742 146 714 4160
Fragments arranged by size:
4160 742 714 146
EcoICRI GAG'CTC
Cuts at: 511 970 1239 1311 2774 511
Size: 459 269 72 1463 3499
Fragments arranged by size:
3499 1463 459 269 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 986 1052 1293 2570 3587 4019 4372 5034
Size: 262 66 241 1277 1017 432 353 662
Cuts at: 5034 724
Size: 1452
Fragments arranged by size:
1452 1277 1017 662 432 353 262 241
66
EcoNI CCTnn'n_nnAGG
Cuts at: 1153 1153
Size: 5762
EcoO109I rG'GnC_Cy
Cuts at: 1145 1146 1253 2574 2901 1145
Size: 1 107 1321 327 4006
Fragments arranged by size:
4006 1321 327 107 1
EcoRI G'AATT_C
Cuts at: 2691 2766 2691
Size: 75 5687
EcoRV GAT'ATC
Cuts at: 1670 1670
Size: 5762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2336 3781 2336
Size: 1445 4317
FspI TGC'GCA
Cuts at: 3543 3543
Size: 5762
HaeII r_GCGC'y
Cuts at: 218 1431 3196 3444 4674 5445 218
Size: 1213 1765 248 1230 771 535
Fragments arranged by size:
1765 1230 1213 771 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 485 517 2046 2078 2113 2145 3604
Size: 32 77 32 1529 32 35 32 1459
Cuts at: 3604 3634 3636 3666 376
Size: 30 2 30 2472
Fragments arranged by size:
2472 1529 1459 77 35 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 941 1449 1496 2801 941
Size: 508 47 1305 3902
Fragments arranged by size:
3902 1305 508 47
KasI G'GCGC_C
Cuts at: 214 3192 3440 214
Size: 2978 248 2536
Fragments arranged by size:
2978 2536 248
KpnI G_GTAC'C
Cuts at: 807 2782 807
Size: 1975 3787
MfeI C'AATT_G
Cuts at: 1499 1595 1499
Size: 96 5666
MluI A'CGCG_T
Cuts at: 5195 5195
Size: 5762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1542 1953 2261 4521 4705 1542
Size: 411 308 2260 184 2599
Fragments arranged by size:
2599 2260 411 308 184
MscI TGG'CCA
Cuts at: 3523 3523
Size: 5762
MslI CAynn'nnrTG
Cuts at: 487 3751 3878 487
Size: 3264 127 2371
Fragments arranged by size:
3264 2371 127
MspA1I CmG'CkG
Cuts at: 2873 3187 3547 4331 4576 2873
Size: 314 360 784 245 4059
Fragments arranged by size:
4059 784 360 314 245
NaeI GCC'GGC
Cuts at: 3943 3943
Size: 5762
NarI GG'CG_CC
Cuts at: 215 3193 3441 215
Size: 2978 248 2536
Fragments arranged by size:
2978 2536 248
NgoMIV G'CCGG_C
Cuts at: 3941 3941
Size: 5762
NsiI A_TGCA'T
Cuts at: 125 125
Size: 5762
NspI r_CATG'y
Cuts at: 2193 2815 3315 4918 2193
Size: 622 500 1603 3037
Fragments arranged by size:
3037 1603 622 500
PasI CC'CwG_GG
Cuts at: 1118 1118
Size: 5762
PciI A'CATG_T
Cuts at: 4914 4914
Size: 5762
PflMI CCAn_nnn'nTGG
Cuts at: 463 463
Size: 5762
PmeI GTTT'AAAC
Cuts at: 2761 2761
Size: 5762
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 5730
PsiI TTA'TAA
Cuts at: 2714 2714
Size: 5762
PspOMI G'GGCC_C
Cuts at: 1145 1252 1145
Size: 107 5655
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 236 268 236
Size: 32 5730
PstI C_TGCA'G
Cuts at: 1461 2809 3494 1461
Size: 1348 685 3729
Fragments arranged by size:
3729 1348 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5762
PvuII CAG'CTG
Cuts at: 3187 3547 3187
Size: 360 5402
RsrII CG'GwC_CG
Cuts at: 3957 3957
Size: 5762
SacI G_AGCT'C
Cuts at: 513 972 1241 1313 2776 513
Size: 459 269 72 1463 3499
Fragments arranged by size:
3499 1463 459 269 72
SalI G'TCGA_C
Cuts at: 2799 2799
Size: 5762
SapI GCTCTTCn'nnn_
Cuts at: 3785 3995 3785
Size: 210 5552
SbfI CC_TGCA'GG
Cuts at: 2809 2809
Size: 5762
ScaI AGT'ACT
Cuts at: 5279 5279
Size: 5762
SexAI A'CCwGG_T
Cuts at: 770 770
Size: 5762
SfcI C'TryA_G
Cuts at: 33 1457 2045 2805 3490 4458 4649 33
Size: 1424 588 760 685 968 191 1146
Fragments arranged by size:
1424 1146 968 760 685 588 191
SfoI GGC'GCC
Cuts at: 216 3194 3442 216
Size: 2978 248 2536
Fragments arranged by size:
2978 2536 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5762
SmaI CCC'GGG
Cuts at: 2784 2784
Size: 5762
SmlI C'TyrA_G
Cuts at: 1339 2917 3832 4546 4808 1339
Size: 1578 915 714 262 2293
Fragments arranged by size:
2293 1578 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5762
SphI G_CATG'C
Cuts at: 2815 3315 2815
Size: 500 5262
SspI AAT'ATT
Cuts at: 2670 2670
Size: 5762
StyI C'CwwG_G
Cuts at: 208 1910 2495 2577 2896 3250 208
Size: 1702 585 82 319 354 2720
Fragments arranged by size:
2720 1702 585 354 319 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1903 3345 3478 3937 4146 1903
Size: 1442 133 459 209 3519
Fragments arranged by size:
3519 1442 459 209 133
TatI w'GTAC_w
Cuts at: 1487 2300 5277 1487
Size: 813 2977 1972
Fragments arranged by size:
2977 1972 813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5279 5311 5279
Size: 32 5730
Tth111I GACn'n_nGTC
Cuts at: 3559 3559
Size: 5762
XbaI T'CTAG_A
Cuts at: 2793 2793
Size: 5762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1115 3257 1115
Size: 2142 3620
XmaI C'CCGG_G
Cuts at: 2782 2782
Size: 5762
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PpiI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AleI AloI AscI BaeI
BbsI BglI BmtI BsgI BsiWI BspEI BsrGI BssHII
BstEII BtgI ClaI DraIII FseI FspAI HindIII HpaI
NcoI NdeI NheI NotI NruI PacI PfoI PmlI
PpuMI PshAI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI